{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8LAJ9","secondaryAccessions":["Q67XY2"],"uniProtkbId":"MIC10_ARATH","entryAudit":{"firstPublicDate":"2022-12-14","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2002-10-01","entryVersion":140,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26898467"}],"value":"MICOS complex subunit MIC10"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26898467"}],"value":"AtMic10"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26898467"}],"value":"MIC10"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT1G22520"}],"value":"At1g22520"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AC006551"}],"value":"F12K8.27"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q5TGZ0"}],"value":"Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q5TGZ0"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26898467"}],"value":"Component of the mitochondrial contact site and cristae organizing system (MICOS) complex (By similarity). The MICOS complex associates with mitochondrial outer membrane proteins (By similarity). Present in a large lipid-enriched complex called mitochondrial transmembrane lipoprotein (MTL) complex made of proteins located in the two mitochondrial membranes, including the TOM complex and the core components of the MICOS complex and containing at least digalactosyldiacylglycerol (DGDG) (PubMed:26898467)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q5TGZ0"}],"value":"Mitochondrion inner membrane","id":"SL-0168"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the MICOS complex subunit Mic10 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"MICOS complex subunit MIC10","featureId":"PRO_0000456902"},{"type":"Transmembrane","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":66,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Sequence conflict","location":{"start":{"value":31,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"in Ref. 4; BAD44449","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["F"]}},{"type":"Sequence conflict","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":69,"modifier":"EXACT"}},"description":"in Ref. 4; BAD44449","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["F"]}},{"type":"Sequence conflict","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"in Ref. 4; BAD44449","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["F"]}}],"keywords":[{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"id":"KW-0999","category":"Cellular component","name":"Mitochondrion inner membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"CI-43D7862RKG1IA","citationType":"submission","authors":["Totoki Y.","Seki M.","Ishida J.","Nakajima M.","Enju A.","Kamiya A.","Narusaka M.","Shin-i T.","Nakagawa M.","Sakamoto N.","Oishi K.","Kohara Y.","Kobayashi M.","Toyoda A.","Sakaki Y.","Sakurai T.","Iida K.","Akiyama K.","Satou M.","Toyoda T.","Konagaya A.","Carninci P.","Kawai J.","Hayashizaki Y.","Shinozaki K."],"title":"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.","publicationDate":"SEP-2004","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-BMBM5MH4O1F9H","citationType":"submission","authors":["Quinitio C.","Chen H.","Kim C.J.","Shinn P.","Ecker J.R."],"title":"Arabidopsis ORF clones.","publicationDate":"MAY-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"CI-3TH3AFLAVIRO9","citationType":"submission","authors":["Brover V.V.","Troukhan M.E.","Alexandrov N.A.","Lu Y.-P.","Flavell R.B.","Feldmann K.A."],"title":"Full-length cDNA from Arabidopsis thaliana.","publicationDate":"MAR-2002","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"]},{"referenceNumber":7,"citation":{"id":"26898467","citationType":"journal article","authors":["Michaud M.","Gros V.","Tardif M.","Brugiere S.","Ferro M.","Prinz W.A.","Toulmay A.","Mathur J.","Wozny M.","Falconet D.","Marechal E.","Block M.A.","Jouhet J."],"citationCrossReferences":[{"database":"PubMed","id":"26898467"},{"database":"DOI","id":"10.1016/j.cub.2016.01.011"}],"title":"AtMic60 is involved in plant mitochondria lipid trafficking and is part of a large complex.","publicationDate":"2016","journal":"Curr. Biol.","firstPage":"627","lastPage":"639","volume":"26"},"referencePositions":["SUBUNIT","IDENTIFICATION BY MASS SPECTROMETRY"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC006551","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE30251.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7028315.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AK176686","properties":[{"key":"ProteinId","value":"BAD44449.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BT025577","properties":[{"key":"ProteinId","value":"ABF58995.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY087767","properties":[{"key":"ProteinId","value":"AAM65303.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_564173.1","properties":[{"key":"NucleotideSequenceId","value":"NM_102101.4"}]},{"database":"AlphaFoldDB","id":"Q8LAJ9","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q8LAJ9","properties":[{"key":"Number of interactors","value":"715"}]},{"database":"SwissPalm","id":"Q8LAJ9","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"179551","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"838858","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G22520","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G22520","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G22520","properties":[{"key":"GeneName","value":"-"}]},{"database":"HOGENOM","id":"CLU_171428_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AYSECSQ","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"1916310at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q8LAJ9","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"Q8LAJ9","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0098800","properties":[{"key":"GoTerm","value":"C:inner mitochondrial membrane protein complex"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"26898467"}]},{"database":"GO","id":"GO:0061617","properties":[{"key":"GoTerm","value":"C:MICOS complex"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0005743","properties":[{"key":"GoTerm","value":"C:mitochondrial inner membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"HDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14671022"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"InterPro","id":"IPR007512","properties":[{"key":"EntryName","value":"Mic10"}]},{"database":"PANTHER","id":"PTHR21304","properties":[{"key":"EntryName","value":"MICOS COMPLEX SUBUNIT MIC10"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR21304:SF13","properties":[{"key":"EntryName","value":"MICOS COMPLEX SUBUNIT MIC10"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04418","properties":[{"key":"EntryName","value":"DUF543"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"METTKSNSSESDVNAKWDACLDLTARRFVYSSLGGAFAGLLFFRSPVTRWASIAFGAGIGIGSAYTDCSRVFDASSSTSATLLAAPKSTETSVSQAAEE","length":99,"molWeight":10357,"crc64":"C282419776C5AA8C","md5":"D1F1F50276CD54AF9EBFC27586085C71"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":2,"Sequence conflict":3},"uniParcId":"UPI00000A5E62"}}