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Associates with the telomeric region, the subtelomeric TARE6 repeat sequence and the var gene promoters (PubMed:22006844)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"a divalent metal cation","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:60240"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22006844"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}],"value":"Mild acetylation lowers protein interaction with DNA and high acetylation abolishes DNA-binding activity (PubMed:22006844). DNA binding and endonuclease activity is modulated via deacetylation of Lys-23 by Sir2A (PubMed:22006844, PubMed:36947546). Inhibited in the presence of EDTA and EGTA (PubMed:36947546)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":9.35E-5,"unit":"uM","substrate":"supercoiled pBR322 DNA","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}],"value":"kcat is 0.00007085 h(-1) for supercoiled pBR322 DNA."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}],"value":"Optimum pH is 7.5-9.0."}]},"temperatureDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}],"value":"Active from 4 to 70 degrees Celsius."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22006844"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22167473"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}],"value":"Homodimer (PubMed:22167473). 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Deacetylated by Sir2A (PubMed:22006844, PubMed:36947546)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the histone-like Alba family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"Endonuclease ALBA3","featureId":"PRO_0000459063"},{"type":"Modified residue","location":{"start":{"value":23,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}]},{"type":"Modified residue","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36947546"}]},{"type":"Mutagenesis","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":23,"modifier":"EXACT"}},"description":"Results in 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