{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8I5I0","uniProtkbId":"VIT_PLAF7","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":111,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Plasmodium falciparum (isolate 3D7)","taxonId":36329,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001450"}],"lineage":["Eukaryota","Sar","Alveolata","Apicomplexa","Aconoidasida","Haemosporida","Plasmodiidae","Plasmodium","Laverania"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26786069"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28198449"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"39575308"}],"value":"Vacuolar iron transporter"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26786069"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28198449"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34193175"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"39575308"}],"value":"PfVIT"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"VIT"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CZT99396.1"}],"value":"PF3D7_1223700"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26786069"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28198449"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"value":"Vacuolar iron transporter involved in the transfer of iron ions from the cytosol to the vacuole for intracellular iron storage (PubMed:26786069, PubMed:34193175). Involved in detoxification of excess iron (PubMed:26786069, PubMed:28198449, PubMed:34193175). The transport mechanism is not well defined and the role of protons is not clear (PubMed:26786069, PubMed:28198449)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Fe(2+)(in) = Fe(2+)(out)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:28486"},{"database":"ChEBI","id":"CHEBI:29033"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26786069"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:28487"},"evidences":[{"evidenceCode":"ECO:0000305"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28198449"}],"value":"Monomer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26786069"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"value":"Vacuole membrane","id":"SL-0271"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A509AT60"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39575308"}],"value":"Cytoplasmic vesicle membrane","id":"SL-0089"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39575308"}],"value":"Expressed in blood stages (at protein level)"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39575308"}],"value":"Expression is modulated by the levels of iron ions in the environment"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"39575308"}],"value":"Targeted gene disruption has no significant effect on blood stage parasite growth under standard conditions (PubMed:39575308). Reduced growth rates in the presence of hepcidin, a specific ferroportin inhibitor causing an increase in labile iron levels in erythrocytes (PubMed:39575308)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26786069"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28198449"}],"value":"Iron transport is insensitive to the proton pump inhibitor bafilomycin A1 or to the proton ionophore CCCP in a yeast heterologous expression system (PubMed:26786069). Functions as a proton-driven antiport in a bacterial heterologous expression system (PubMed:28198449)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CCC1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":273,"modifier":"EXACT"}},"description":"Vacuolar iron transporter","featureId":"PRO_0000459389"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":47,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"Vacuolar","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":97,"modifier":"EXACT"},"end":{"value":181,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":182,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":212,"modifier":"EXACT"}},"description":"Vacuolar","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":213,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":234,"modifier":"EXACT"},"end":{"value":246,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":247,"modifier":"EXACT"},"end":{"value":267,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":268,"modifier":"EXACT"},"end":{"value":273,"modifier":"EXACT"}},"description":"Vacuolar","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Binding site","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"1"}},{"type":"Binding site","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"2"}},{"type":"Binding site","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"1"}},{"type":"Binding site","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"3"}},{"type":"Binding site","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"1"}},{"type":"Binding site","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"2"}},{"type":"Binding site","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"3"}},{"type":"Binding site","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"1"}},{"type":"Binding site","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"2"}},{"type":"Binding site","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"3"}},{"type":"Binding site","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"2"}},{"type":"Binding site","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0DO17"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"1"}},{"type":"Mutagenesis","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"No effect on iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Reduces iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"No effect on iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":116,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"Reduces iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":124,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"No effect on iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A","Q"]}},{"type":"Mutagenesis","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"Slightly increases iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"Reduces iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":161,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"Reduces iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["A","L","C","E"]}},{"type":"Mutagenesis","location":{"start":{"value":162,"modifier":"EXACT"},"end":{"value":162,"modifier":"EXACT"}},"description":"No effect on iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"M","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"Reduces iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":165,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"No effect on iron tolerance in a yeast heterologous expression system.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34193175"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}}],"keywords":[{"id":"KW-0968","category":"Cellular component","name":"Cytoplasmic vesicle"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0410","category":"Biological process","name":"Iron transport"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0926","category":"Cellular component","name":"Vacuole"}],"references":[{"referenceNumber":1,"citation":{"id":"12368864","citationType":"journal article","authors":["Gardner M.J.","Hall N.","Fung E.","White O.","Berriman M.","Hyman R.W.","Carlton J.M.","Pain A.","Nelson K.E.","Bowman S.","Paulsen I.T.","James K.D.","Eisen J.A.","Rutherford K.M.","Salzberg S.L.","Craig A.","Kyes S.","Chan M.-S.","Nene V.","Shallom S.J.","Suh B.","Peterson J.","Angiuoli S.","Pertea M.","Allen J.","Selengut J.","Haft D.","Mather M.W.","Vaidya A.B.","Martin D.M.A.","Fairlamb A.H.","Fraunholz M.J.","Roos D.S.","Ralph S.A.","McFadden G.I.","Cummings L.M.","Subramanian G.M.","Mungall C.","Venter J.C.","Carucci D.J.","Hoffman S.L.","Newbold C.","Davis R.W.","Fraser C.M.","Barrell B.G."],"citationCrossReferences":[{"database":"PubMed","id":"12368864"},{"database":"DOI","id":"10.1038/nature01097"}],"title":"Genome sequence of the human malaria parasite Plasmodium falciparum.","publicationDate":"2002","journal":"Nature","firstPage":"498","lastPage":"511","volume":"419"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001450"}],"value":"3D7","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001450"}]},{"referenceNumber":2,"citation":{"id":"26786069","citationType":"journal article","authors":["Slavic K.","Krishna S.","Lahree A.","Bouyer G.","Hanson K.K.","Vera I.","Pittman J.K.","Staines H.M.","Mota M.M."],"citationCrossReferences":[{"database":"PubMed","id":"26786069"},{"database":"DOI","id":"10.1038/ncomms10403"}],"title":"A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium.","publicationDate":"2016","journal":"Nat. Commun.","firstPage":"10403","lastPage":"10403","volume":"7"},"referencePositions":["FUNCTION","TRANSPORTER ACTIVITY","SUBCELLULAR LOCATION"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":3,"citation":{"id":"28198449","citationType":"journal article","authors":["Labarbuta P.","Duckett K.","Botting C.H.","Chahrour O.","Malone J.","Dalton J.P.","Law C.J."],"citationCrossReferences":[{"database":"PubMed","id":"28198449"},{"database":"DOI","id":"10.1038/srep42850"}],"title":"Recombinant vacuolar iron transporter family homologue PfVIT from human malaria-causing Plasmodium falciparum is a Fe2+/H+exchanger.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"42850","lastPage":"42850","volume":"7"},"referencePositions":["FUNCTION","SUBUNIT"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":4,"citation":{"id":"34193175","citationType":"journal article","authors":["Sharma P.","Toth V.","Hyland E.M.","Law C.J."],"citationCrossReferences":[{"database":"PubMed","id":"34193175"},{"database":"DOI","id":"10.1186/s12936-021-03827-7"}],"title":"Characterization of the substrate binding site of an iron detoxifying membrane transporter from Plasmodium falciparum.","publicationDate":"2021","journal":"Malar. J.","firstPage":"295","lastPage":"295","volume":"20"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","MUTAGENESIS OF GLU-113; GLU-116; GLU-124; GLU-127; MET-161; MET-162 AND GLU-165"],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":5,"citation":{"id":"39575308","citationType":"journal article","authors":["Wunderlich J.","Kotov V.","Votborg-Novel L.","Ntalla C.","Geffken M.","Peine S.","Portugal S.","Strauss J."],"citationCrossReferences":[{"database":"PubMed","id":"39575308"},{"database":"DOI","id":"10.3389/fcimb.2024.1480076"}],"title":"Iron transport pathways in the human malaria parasite Plasmodium falciparum revealed by RNA-sequencing.","publicationDate":"2024","journal":"Front. Cell. Infect. Microbiol.","firstPage":"1480076","lastPage":"1480076","volume":"14"},"referencePositions":["SUBCELLULAR LOCATION","DEVELOPMENTAL STAGE","INDUCTION","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"LN999947","properties":[{"key":"ProteinId","value":"CZT99396.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"XP_001350634.1","properties":[{"key":"NucleotideSequenceId","value":"XM_001350598.1"}]},{"database":"AlphaFoldDB","id":"Q8I5I0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8I5I0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"36329.Q8I5I0","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"2.A.89.1.13","properties":[{"key":"FamilyName","value":"the vacuolar iron transporter (vit) family"}]},{"database":"PaxDb","id":"36329-Q8I5I0","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblProtists","id":"PF3D7_1223700.1","properties":[{"key":"ProteinId","value":"PF3D7_1223700.1-p1"},{"key":"GeneId","value":"PF3D7_1223700"}]},{"database":"GeneID","id":"811280","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"pfa:PF3D7_1223700","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"PlasmoDB:PF3D7_1223700","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_038957_2_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8I5I0","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"SRIGWLR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"73465at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8I5I0","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001450","properties":[{"key":"Component","value":"Chromosome 12"}]},{"database":"GO","id":"GO:0030659","properties":[{"key":"GoTerm","value":"C:cytoplasmic vesicle membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005789","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005774","properties":[{"key":"GoTerm","value":"C:vacuolar membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005384","properties":[{"key":"GoTerm","value":"F:manganese ion transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0030026","properties":[{"key":"GoTerm","value":"P:intracellular manganese ion homeostasis"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd02434","properties":[{"key":"EntryName","value":"Nodulin-21_like_3"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR008217","properties":[{"key":"EntryName","value":"Ccc1_fam"}]},{"database":"PANTHER","id":"PTHR31851","properties":[{"key":"EntryName","value":"FE(2+)/MN(2+) TRANSPORTER PCL1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01988","properties":[{"key":"EntryName","value":"VIT1"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MVSKKTIEARKAYYNEDVVLSKEAHDFYHNLDKHGENHNLDKDNLKTIIFGSLDGIITIFAIVSGCVGAKITPTQVIIIGIGNLFANAISMGFSEYTSSTAQRDFMLAEKKREEWEIENCPSEEKQEMIDIYMNKYKFDSEDARNLVEITFRNKNFFLEHMMSEELGLIVTNEDKNECLKKGIIMFLSFAVFGIIPLSAYVAYTVFFGYTDYTTSFLVVFISTLTTLFILGLFKSQFTNQKPITCALYMVLNGMIAGMVPFLLGVVLKNNISE","length":273,"molWeight":30984,"crc64":"6B349FA23FC60728","md5":"DFD8CA3A915F4E0B15B5325542376D5F"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DEVELOPMENTAL STAGE":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":6,"Transmembrane":5,"Binding site":12,"Mutagenesis":11},"uniParcId":"UPI0000085F29"}}