{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8H1E7","secondaryAccessions":["Q93ZS0","Q9FZ64"],"uniProtkbId":"NOF1_ARATH","entryAudit":{"firstPublicDate":"2022-02-23","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":137,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20877469"}],"value":"Protein NUCLEOLAR FACTOR 1"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"20877469"}],"value":"NOF1"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AEE29622.1"}],"value":"At1g17690"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAF99813.1"}],"value":"F11A6.3"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P40498"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20877469"}],"value":"DEAD-box RNA helicase-like protein required for pre-18S rRNA processing, specifically at sites A0, A1, and A2 (By similarity). Involved in the control of rRNA expression (PubMed:20877469). Required for embryo development and female gametogenesis (PubMed:20877469)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P40498"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27792779"}],"value":"Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3 (By similarity). Interacts with THAL in the nucleus (PubMed:27792779)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20877469"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27792779"}],"value":"Nucleus, nucleolus","id":"SL-0188"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20877469"}],"value":"Preferentially expressed in differentiating cells in young tissues such as floral buds, ovules, embryos, secondary roots, pollen, young seedlings and vascular bundles (PubMed:20877469). Observed ubiquitously (PubMed:20877469)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20877469"}],"value":"Abnormal female gametogenesis and altered embryo development with a disturbed cellular division pattern (PubMed:20877469). Increased rRNA expression and hypomethylation of rRNA promoters (PubMed:20877469)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UTP25 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"AAF99813.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":754,"modifier":"EXACT"}},"description":"Protein NUCLEOLAR FACTOR 1","featureId":"PRO_0000454728"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":56,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":69,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":390,"modifier":"EXACT"},"end":{"value":413,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":93,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":138,"modifier":"EXACT"},"end":{"value":161,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":402,"modifier":"EXACT"},"end":{"value":413,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Sequence conflict","location":{"start":{"value":732,"modifier":"EXACT"},"end":{"value":732,"modifier":"EXACT"}},"description":"in Ref. 4; AAL07149","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["G"]}}],"keywords":[{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0687","category":"Molecular function","name":"Ribonucleoprotein"},{"id":"KW-0690","category":"Biological process","name":"Ribosome biogenesis"},{"id":"KW-0698","category":"Biological process","name":"rRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"20877469","citationType":"journal article","authors":["Harscoet E.","Dubreucq B.","Palauqui J.-C.","Lepiniec L."],"citationCrossReferences":[{"database":"PubMed","id":"20877469"},{"database":"DOI","id":"10.1371/journal.pone.0012829"}],"title":"NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.","publicationDate":"2010","journal":"PLoS ONE","firstPage":"e12829","lastPage":"e12829","volume":"5"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","TISSUE SPECIFICITY","SUBCELLULAR LOCATION"],"referenceComments":[{"value":"cv. Wassilewskija","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"27792779","citationType":"journal article","authors":["Chen Y.-J.C.","Wang H.-J.","Jauh G.-Y."],"citationCrossReferences":[{"database":"PubMed","id":"27792779"},{"database":"DOI","id":"10.1371/journal.pgen.1006408"}],"title":"Dual role of a SAS10/C1D family protein in ribosomal RNA gene expression and processing is essential for reproduction in Arabidopsis thaliana.","publicationDate":"2016","journal":"PLoS Genet.","firstPage":"e1006408","lastPage":"e1006408","volume":"12"},"referencePositions":["SUBCELLULAR LOCATION","INTERACTION WITH THAL"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC034257","properties":[{"key":"ProteinId","value":"AAF99813.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE29622.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7027740.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AY056300","properties":[{"key":"ProteinId","value":"AAL07149.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY150441","properties":[{"key":"ProteinId","value":"AAN12983.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"G86311","properties":[{"key":"EntryName","value":"G86311"}]},{"database":"RefSeq","id":"NP_564032.1","properties":[{"key":"NucleotideSequenceId","value":"NM_101631.2"}]},{"database":"AlphaFoldDB","id":"Q8H1E7","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q8H1E7","properties":[{"key":"Number of interactors","value":"4484"}]},{"database":"IntAct","id":"Q8H1E7","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"3702.Q8H1E7","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q8H1E7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q8H1E7","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"193307","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"838345","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G17690","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G17690","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G17690","properties":[{"key":"GeneName","value":"NOF1"}]},{"database":"eggNOG","id":"KOG2340","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_018705_2_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8H1E7","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"QDRGDTF","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10264378at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8H1E7","properties":[{"key":"Description","value":"-"}]},{"database":"CD-CODE","id":"4299E36E","properties":[{"key":"EntryName","value":"Nucleolus"}]},{"database":"PRO","id":"PR:Q8H1E7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"Q8H1E7","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005730","properties":[{"key":"GoTerm","value":"C:nucleolus"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"20877469"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"27792779"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0032040","properties":[{"key":"GoTerm","value":"C:small-subunit processome"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0019843","properties":[{"key":"GoTerm","value":"F:rRNA binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0034511","properties":[{"key":"GoTerm","value":"F:U3 snoRNA binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0009793","properties":[{"key":"GoTerm","value":"P:embryo development ending in seed dormancy"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20877469"}]},{"database":"GO","id":"GO:0009553","properties":[{"key":"GoTerm","value":"P:embryo sac development"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20877469"}]},{"database":"GO","id":"GO:0000462","properties":[{"key":"GoTerm","value":"P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0009303","properties":[{"key":"GoTerm","value":"P:rRNA transcription"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"20877469"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR010678","properties":[{"key":"EntryName","value":"UTP25"}]},{"database":"InterPro","id":"IPR053939","properties":[{"key":"EntryName","value":"UTP25_C"}]},{"database":"InterPro","id":"IPR053940","properties":[{"key":"EntryName","value":"UTP25_NTPase-like"}]},{"database":"PANTHER","id":"PTHR12933","properties":[{"key":"EntryName","value":"ORF PROTEIN-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12933:SF0","properties":[{"key":"EntryName","value":"U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 25 HOMOLOG"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06862","properties":[{"key":"EntryName","value":"Utp25_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22916","properties":[{"key":"EntryName","value":"UTP25_NTPase-like"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAPNATGVLKRHRSHEKFDKKRDTKKHKHVEKTIVSNPSTDSPEESSIEAESEAMVYREPTQYQNLLVSLGSSNKVVADMNKRRQREEEGKSDTEEDEDDEDEDEEENSGSDDLSSTDGEDDKSQGDDQETLGGLTDDTQEDNDNQSEEEDPDDYETDEEVHELSTNGQSFVDASSSISAFSEHLSHKLSSEEVETLPKGKWKFKWESPAFDMPNCKWKGTSENFLDGIQSDAPYGLKPKLYNHWLQLYKKCGGKDLDSSKRRKFFSICNSYLDILHSNKKPFYHCGSDEDSSAMDAYLMHSLNHIFKTRDLVKKNESKIAKHRETSEEEILSDDGFLDQGFTRPKVLILLPLRSIAFRVVKRLIQLTPESQRVNVEHLDRFNDEFGCEEDTDDCDGEKTTSKNGNSIKQKSSKPSDWQALFGANNNDDEFMLGIKHTRKSIRLYGDFYSSDIIVASPLKLHMAIGAAEENKERDVDYLSSIEVLVIDHADIISMQNWSFLATVVDYLNRLPTKQHGTNVMRIRPLYLDGHARFYRQSIILSSYLTPEMNSLFGRHCLNYKGKMKMACEYKGVLEKVLLPVRQIYERFDAASITQVDDARLEYFTKKIFPKIKDSVQGGVMIFIHSYFEFVRLRNFLNTQNTSFCLLGDYAKNADISRAREQFFVGSRKIMLYTERAYFYKRYKIRGIKNLILYSLPERKEFYPEIMNMLEEGSHDMMSTALFSRFDMLQLERIVGSTSAKRMITSEKNMFAFC","length":754,"molWeight":86920,"crc64":"93B7397E56468E9F","md5":"0CBCCE8F720BE7D1969CA12BEAC445E6"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Region":3,"Compositional bias":5,"Sequence conflict":1},"uniParcId":"UPI00000ADA92"}}