{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8GWX2","secondaryAccessions":["Q9SKQ1"],"uniProtkbId":"NIPA6_ARATH","entryAudit":{"firstPublicDate":"2014-10-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":122,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Probable magnesium transporter NIPA6"}}},"genes":[{"orderedLocusNames":[{"value":"At2g21120"}],"orfNames":[{"value":"F26H11.12"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Fe(2+), Sr(2+), Ba(2+), Mn(2+) and Co(2+) but to a much less extent than Mg(2+) (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Recruited to the cell membrane in response to low extracellular magnesium"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Multi-pass membrane protein","id":"SL-9909"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Early endosome","id":"SL-0094"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the NIPA (TC 2.A.7) family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"AAD29804.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"Probable magnesium transporter NIPA6","featureId":"PRO_0000430294"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"Helical; Name=1","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"Helical; Name=2","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":76,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":97,"modifier":"EXACT"}},"description":"Helical; Name=3","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":98,"modifier":"EXACT"},"end":{"value":104,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":105,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"Helical; Name=4","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":143,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Helical; Name=5","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":164,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":176,"modifier":"EXACT"},"end":{"value":196,"modifier":"EXACT"}},"description":"Helical; Name=6","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":197,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":210,"modifier":"EXACT"},"end":{"value":230,"modifier":"EXACT"}},"description":"Helical; Name=7","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":231,"modifier":"EXACT"},"end":{"value":240,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"Helical; Name=8","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":262,"modifier":"EXACT"},"end":{"value":269,"modifier":"EXACT"}},"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":270,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Helical; Name=9","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":291,"modifier":"EXACT"},"end":{"value":328,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0406","category":"Biological process","name":"Ion transport"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"10617197","citationType":"journal article","authors":["Lin X.","Kaul S.","Rounsley S.D.","Shea T.P.","Benito M.-I.","Town C.D.","Fujii C.Y.","Mason T.M.","Bowman C.L.","Barnstead M.E.","Feldblyum T.V.","Buell C.R.","Ketchum K.A.","Lee J.J.","Ronning C.M.","Koo H.L.","Moffat K.S.","Cronin L.A.","Shen M.","Pai G.","Van Aken S.","Umayam L.","Tallon L.J.","Gill J.E.","Adams M.D.","Carrera A.J.","Creasy T.H.","Goodman H.M.","Somerville C.R.","Copenhaver G.P.","Preuss D.","Nierman W.C.","White O.","Eisen J.A.","Salzberg S.L.","Fraser C.M.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"10617197"},{"database":"DOI","id":"10.1038/45471"}],"title":"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.","publicationDate":"1999","journal":"Nature","firstPage":"761","lastPage":"768","volume":"402"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"11910074","citationType":"journal article","authors":["Seki M.","Narusaka M.","Kamiya A.","Ishida J.","Satou M.","Sakurai T.","Nakajima M.","Enju A.","Akiyama K.","Oono Y.","Muramatsu M.","Hayashizaki Y.","Kawai J.","Carninci P.","Itoh M.","Ishii Y.","Arakawa T.","Shibata K.","Shinagawa A.","Shinozaki K."],"citationCrossReferences":[{"database":"PubMed","id":"11910074"},{"database":"DOI","id":"10.1126/science.1071006"}],"title":"Functional annotation of a full-length Arabidopsis cDNA collection.","publicationDate":"2002","journal":"Science","firstPage":"141","lastPage":"145","volume":"296"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-8S3SL9B1A2RMU","citationType":"submission","authors":["Kim C.J.","Chen H.","Quinitio C.","Shinn P.","Ecker J.R."],"title":"Arabidopsis ORF clones.","publicationDate":"JUL-2006","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC006264","properties":[{"key":"ProteinId","value":"AAD29804.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002685","properties":[{"key":"ProteinId","value":"AEC07125.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408684","properties":[{"key":"ProteinId","value":"CAO7035771.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AK118581","properties":[{"key":"ProteinId","value":"BAC43181.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BT026119","properties":[{"key":"ProteinId","value":"ABG48475.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"D84597","properties":[{"key":"EntryName","value":"D84597"}]},{"database":"RefSeq","id":"NP_179708.2","properties":[{"key":"NucleotideSequenceId","value":"NM_127682.4"}]},{"database":"AlphaFoldDB","id":"Q8GWX2","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q8GWX2","properties":[{"key":"Number of interactors","value":"4089"}]},{"database":"STRING","id":"3702.Q8GWX2","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q8GWX2","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q8GWX2","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"250534","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"816647","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT2G21120","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT2G21120","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT2G21120","properties":[{"key":"GeneName","value":"AVI2"}]},{"database":"eggNOG","id":"KOG2922","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_012349_1_1_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8GWX2","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"IYVAITM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"6428174at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8GWX2","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q8GWX2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 2"}]},{"database":"ExpressionAtlas","id":"Q8GWX2","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0005769","properties":[{"key":"GoTerm","value":"C:early endosome"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"17166836"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"29184028"}]},{"database":"GO","id":"GO:0016020","properties":[{"key":"GoTerm","value":"C:membrane"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"17166836"}]},{"database":"GO","id":"GO:0015095","properties":[{"key":"GoTerm","value":"F:magnesium ion transmembrane transporter activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0051607","properties":[{"key":"GoTerm","value":"P:defense response to virus"},{"key":"GoEvidenceType","value":"IGI:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000316","source":"PubMed","id":"29184028"},{"evidenceCode":"ECO:0000316","source":"PubMed","id":"29396140"}]},{"database":"GO","id":"GO:0015693","properties":[{"key":"GoTerm","value":"P:magnesium ion transport"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"17166836"}]},{"database":"InterPro","id":"IPR037185","properties":[{"key":"EntryName","value":"EmrE-like"}]},{"database":"InterPro","id":"IPR008521","properties":[{"key":"EntryName","value":"Mg_trans_NIPA"}]},{"database":"PANTHER","id":"PTHR12570","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12570:SF11","properties":[{"key":"EntryName","value":"MAGNESIUM TRANSPORTER NIPA6-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05653","properties":[{"key":"EntryName","value":"Mg_trans_NIPA"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103481","properties":[{"key":"EntryName","value":"Multidrug resistance efflux transporter EmrE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"METDNGKGLILAVASSVFIGSSFILKKKGLKRAGAIGTRAGYGGYTYLLEPLWWAGMVTMIVGEAANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKEKLKKMGVLGCVSCIVGSVVIVIHAPKEQTPNSVEEIWNLATQPAFLIYVAITMSIVLALILHFEPLCGQTNILVYIGICSLMGALTVMSIKAIGIAIKLTMEGVSQIGYPQTWLFVMVAVTCVVTQLIYLNKALDTFNAAIVSPVYYVMFTTLTIVASAIMFKDWSGQDAASVASELCGFITVLTGTMILHGTREEEQQQASSEHVRWYDSRKSMNEEHLVSLYSPEY","length":328,"molWeight":35580,"crc64":"EBDF986F88E3BDB3","md5":"3F3AAC725BB03EEBCE23D9EF1EFC5E39"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Topological domain":10,"Transmembrane":9},"uniParcId":"UPI00000ADF5B"}}