{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8GWQ6","secondaryAccessions":["A8MRN7","Q944I0","Q9FMV8"],"uniProtkbId":"Y6344_ARATH","entryAudit":{"firstPublicDate":"2014-07-09","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":131,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"UPF0235 protein At5g63440"}}},"genes":[{"orderedLocusNames":[{"value":"At5g63440"}],"orfNames":[{"value":"MLE2.7"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"May play a role during early embryonic development. Probably involved in pre-mRNA splicing (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23454656"}],"value":"Interacts with CTN"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q8GWQ6","intActId":"EBI-8454619"},"interactantTwo":{"uniProtKBAccession":"F4I2J8","geneName":"CTN","intActId":"EBI-8454629"},"numberOfExperiments":4,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23454656"}],"value":"Nucleus speckle","id":"SL-0186"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q8GWQ6-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q8GWQ6-2"],"sequenceIds":["VSP_054999","VSP_055000"],"isoformSequenceStatus":"Described"},{"name":{"value":"3"},"isoformIds":["Q8GWQ6-3"],"sequenceIds":["VSP_054998"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23454656"}],"value":"No visible phenotype"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the UPF0235 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"BAB08809.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"UPF0235 protein At5g63440","featureId":"PRO_0000429605"},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":41,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000305"}],"featureId":"VSP_054998","alternativeSequence":{"originalSequence":"MPKRTTHTYSSEDAAPDGPDSDLFVYYCKHCGSHVLITDTQ","alternativeSequences":["MDPTLISSYTTANTVVLMSLSL"]}},{"type":"Alternative sequence","location":{"start":{"value":192,"modifier":"EXACT"},"end":{"value":205,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14593172"}],"featureId":"VSP_054999","alternativeSequence":{"originalSequence":"LGLRLSQMTLQRGW","alternativeSequences":["PNQSVINHFLRFIL"]}},{"type":"Alternative sequence","location":{"start":{"value":206,"modifier":"EXACT"},"end":{"value":232,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"14593172"}],"featureId":"VSP_055000","alternativeSequence":{}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0507","category":"Biological process","name":"mRNA processing"},{"id":"KW-0508","category":"Biological process","name":"mRNA splicing"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0747","category":"Cellular component","name":"Spliceosome"}],"references":[{"referenceNumber":1,"citation":{"id":"9501997","citationType":"journal article","authors":["Nakamura Y.","Sato S.","Kaneko T.","Kotani H.","Asamizu E.","Miyajima N.","Tabata S."],"citationCrossReferences":[{"database":"PubMed","id":"9501997"},{"database":"DOI","id":"10.1093/dnares/4.6.401"}],"title":"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones.","publicationDate":"1997","journal":"DNA Res.","firstPage":"401","lastPage":"414","volume":"4"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"11910074","citationType":"journal article","authors":["Seki M.","Narusaka M.","Kamiya A.","Ishida J.","Satou M.","Sakurai T.","Nakajima M.","Enju A.","Akiyama K.","Oono Y.","Muramatsu M.","Hayashizaki Y.","Kawai J.","Carninci P.","Itoh M.","Ishii Y.","Arakawa T.","Shibata K.","Shinagawa A.","Shinozaki K."],"citationCrossReferences":[{"database":"PubMed","id":"11910074"},{"database":"DOI","id":"10.1126/science.1071006"}],"title":"Functional annotation of a full-length Arabidopsis cDNA collection.","publicationDate":"2002","journal":"Science","firstPage":"141","lastPage":"145","volume":"296"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"23454656","citationType":"journal article","authors":["Baldwin K.L.","Dinh E.M.","Hart B.M.","Masson P.H."],"citationCrossReferences":[{"database":"PubMed","id":"23454656"},{"database":"DOI","id":"10.1016/j.febslet.2013.02.041"}],"title":"CACTIN is an essential nuclear protein in Arabidopsis and may be associated with the eukaryotic spliceosome.","publicationDate":"2013","journal":"FEBS Lett.","firstPage":"873","lastPage":"879","volume":"587"},"referencePositions":["SUBCELLULAR LOCATION","INTERACTION WITH CTN","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB007649","properties":[{"key":"ProteinId","value":"BAB08809.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002688","properties":[{"key":"ProteinId","value":"AED97747.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002688","properties":[{"key":"ProteinId","value":"AED97748.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002688","properties":[{"key":"ProteinId","value":"AED97749.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408687","properties":[{"key":"ProteinId","value":"CAO7057610.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408687","properties":[{"key":"ProteinId","value":"CAO7057611.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AK118693","properties":[{"key":"ProteinId","value":"BAC43287.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AF428410","properties":[{"key":"ProteinId","value":"AAL16178.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY133627","properties":[{"key":"ProteinId","value":"AAM91457.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001078789.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001085320.1"}],"isoformId":"Q8GWQ6-3"},{"database":"RefSeq","id":"NP_568972.2","properties":[{"key":"NucleotideSequenceId","value":"NM_125739.4"}],"isoformId":"Q8GWQ6-1"},{"database":"RefSeq","id":"NP_851256.1","properties":[{"key":"NucleotideSequenceId","value":"NM_180925.2"}],"isoformId":"Q8GWQ6-2"},{"database":"AlphaFoldDB","id":"Q8GWQ6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8GWQ6","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"21705","properties":[{"key":"Interactions","value":"1"}]},{"database":"FunCoup","id":"Q8GWQ6","properties":[{"key":"Number of interactors","value":"3821"}]},{"database":"IntAct","id":"Q8GWQ6","properties":[{"key":"Interactions","value":"1"}]},{"database":"MINT","id":"Q8GWQ6","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"3702.Q8GWQ6","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q8GWQ6","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"243172","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8GWQ6-1"},{"database":"GeneID","id":"836463","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT5G63440","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT5G63440","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT5G63440","properties":[{"key":"GeneName","value":"CSU4"}]},{"database":"eggNOG","id":"KOG3276","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"eggNOG","id":"KOG4397","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_075663_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8GWQ6","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"CSGCELF","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"418131at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8GWQ6","properties":[{"key":"Description","value":"-"}]},{"database":"CD-CODE","id":"4299E36E","properties":[{"key":"EntryName","value":"Nucleolus"}]},{"database":"PRO","id":"PR:Q8GWQ6","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 5"}]},{"database":"ExpressionAtlas","id":"Q8GWQ6","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0016607","properties":[{"key":"GoTerm","value":"C:nuclear speck"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23454656"}]},{"database":"GO","id":"GO:0097167","properties":[{"key":"GoTerm","value":"P:circadian regulation of translation"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"30352855"}]},{"database":"GO","id":"GO:0009640","properties":[{"key":"GoTerm","value":"P:photomorphogenesis"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"30352855"}]},{"database":"GO","id":"GO:2000306","properties":[{"key":"GoTerm","value":"P:positive regulation of photomorphogenesis"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"30352855"}]},{"database":"FunFam","id":"3.30.1200.10:FF:000001","properties":[{"key":"EntryName","value":"UPF0235 protein isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1200.10","properties":[{"key":"EntryName","value":"YggU-like"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00634","properties":[{"key":"EntryName","value":"UPF0235"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR003746","properties":[{"key":"EntryName","value":"DUF167"}]},{"database":"InterPro","id":"IPR057965","properties":[{"key":"EntryName","value":"STEEP1_dom"}]},{"database":"InterPro","id":"IPR053323","properties":[{"key":"EntryName","value":"UPF0235"}]},{"database":"InterPro","id":"IPR036591","properties":[{"key":"EntryName","value":"YggU-like_sf"}]},{"database":"NCBIfam","id":"TIGR00251","properties":[{"key":"EntryName","value":"DUF167 family protein"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47525","properties":[{"key":"EntryName","value":"OS07G0295200 PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR47525:SF1","properties":[{"key":"EntryName","value":"OS07G0295200 PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02594","properties":[{"key":"EntryName","value":"PF1765-like"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF25809","properties":[{"key":"EntryName","value":"STEEP1"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01152","properties":[{"key":"EntryName","value":"DUF167"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF69786","properties":[{"key":"EntryName","value":"YggU-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPKRTTHTYSSEDAAPDGPDSDLFVYYCKHCGSHVLITDTQLQKMPKRKTDRSNVLDKKTHLARLNVSEGGKVLLKRGEGKMERQFRMNCIGCELFVCYRAEENLETASFIYIVDGALSAVAAETNPQDAPVPPCISQLDGGLVQVAIEVEDRAQRSAITRVNADDVRVTVAAPAARGEANNELLEFMGRVLGLRLSQMTLQRGWNSKSKLLVVEDLSARQVYEKLLEAVVP","length":232,"molWeight":25693,"crc64":"8A6574783E9BDC26","md5":"FB7D3524ECE2FA7730AD2B06E78C8CFE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":3,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Alternative sequence":3},"uniParcId":"UPI00000ADF77"}}