{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8DQ15","uniProtkbId":"ROCS_STRR6","entryAudit":{"firstPublicDate":"2022-12-14","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":104,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Streptococcus pneumoniae (strain ATCC BAA-255 / R6)","taxonId":171101,"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAK99699.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000000586"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Lactobacillales","Streptococcaceae","Streptococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31182798"}],"value":"Regulator of chromosome segregation"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31182798"}],"value":"rocS"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAK99699.1"}],"value":"spr0895"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Required for cell division and chromosome segregation. Binds to DNA and is involved in segregating the origin of replication (oriC) region to new daughter cells"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0H2ZKY7"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Interacts with ParB (By similarity). Interacts with FtsZ in vitro (PubMed:31182798)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Localizes at mid-cell at first and then at the future site of division as the cell elongates. Forms one or two bright foci per cell mostly around mid-cell of small cells. Localizes toward the center of the daughter cell as the cell elongates. The bright foci mostly co-localize with origin of replication (oriC). During the cell cycle, very faint, but highly dynamic foci are homogeneously distributed all around the cell periphery"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Cytoplasm, nucleoid","id":"SL-0187"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Cell membrane","id":"SL-0039"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"The N-terminal DNA-binding helix-turn-helix (HTH) domain and the C-terminal membrane-binding amphipathic helix (AH) are both required for the function in chromosome segregation and for its proper subcellular location"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"value":"Nucleoid defects. 15.7% of the cells are anucleate. Unaffected localization of the divisome. Chromosome-partitioning defects. Newly replicated chromosomes are not segregated (in 7% of the cells) or partially segregated and eventually truncated by the newly forming septum (in 21.8% of the cells). Segregation defects result in small cells, which constitute the majority of the anucleate cells (86.3%). No defects in chromosome replication. Chromosome-pinching takes place as nucleoid is asymmetrically distributed in elongated and constricting cells. Origin of replication (oriC) normally localizes as a single focus located around mid-cell of a nascent cell, but in cells lacking this gene, localization of oriC is strongly affected throughout the cell cycle and after duplication of the focus, most of the two foci remain near mid-cell and do not segregate to the daugher cells as in wild-type. The proportion of cells with only single foci is also significantly higher than in wild-type cells"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"Regulator of chromosome segregation","featureId":"PRO_0000456761"},{"type":"Mutagenesis","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":15,"modifier":"EXACT"}},"description":"Nearly complete loss of DNA-binding.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31182798"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["P"]}}],"keywords":[{"id":"KW-0131","category":"Biological process","name":"Cell cycle"},{"id":"KW-0132","category":"Biological process","name":"Cell division"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0159","category":"Biological process","name":"Chromosome partition"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"11544234","citationType":"journal article","authors":["Hoskins J.","Alborn W.E. 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Bacteriol.","firstPage":"5709","lastPage":"5717","volume":"183"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000000586"}],"value":"ATCC BAA-255 / R6","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAK99699.1"},{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000000586"}]},{"referenceNumber":2,"citation":{"id":"31182798","citationType":"journal article","authors":["Mercy C.","Ducret A.","Slager J.","Lavergne J.P.","Freton C.","Nagarajan S.N.","Garcia P.S.","Noirot-Gros M.F.","Dubarry N.","Nourikyan J.","Veening J.W.","Grangeasse C."],"citationCrossReferences":[{"database":"PubMed","id":"31182798"},{"database":"DOI","id":"10.1038/s41564-019-0472-z"}],"title":"RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae.","publicationDate":"2019","journal":"Nat. Microbiol.","firstPage":"1661","lastPage":"1670","volume":"4"},"referencePositions":["FUNCTION","INTERACTION WITH FTSZ","SUBCELLULAR LOCATION","DOMAIN","DISRUPTION PHENOTYPE","MUTAGENESIS OF GLY-15"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31182798"}],"value":"R6 / R800","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE007317","properties":[{"key":"ProteinId","value":"AAK99699.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"F95114","properties":[{"key":"EntryName","value":"F95114"}]},{"database":"PIR","id":"G97983","properties":[{"key":"EntryName","value":"G97983"}]},{"database":"RefSeq","id":"WP_000021242.1","properties":[{"key":"NucleotideSequenceId","value":"NC_003098.1"}]},{"database":"AlphaFoldDB","id":"Q8DQ15","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8DQ15","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"171101.spr0895","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"171101-spr0895","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"45218287","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"93739471","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"spr:spr0895","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|171101.6.peg.982","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"ENOG5033BIW","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_1561956_0_0_9","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000586","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0043590","properties":[{"key":"GoTerm","value":"C:bacterial nucleoid"},{"key":"GoEvidenceType","value":"IEA:GOC"}]},{"database":"GO","id":"GO:0032153","properties":[{"key":"GoTerm","value":"C:cell division site"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31182798"}]},{"database":"GO","id":"GO:0009295","properties":[{"key":"GoTerm","value":"C:nucleoid"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31182798"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"31182798"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31182798"}]},{"database":"GO","id":"GO:0036386","properties":[{"key":"GoTerm","value":"P:bacterial nucleoid DNA packaging"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31182798"}]},{"database":"GO","id":"GO:2000245","properties":[{"key":"GoTerm","value":"P:negative regulation of FtsZ-dependent cytokinesis"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"31182798"}]},{"database":"InterPro","id":"IPR007489","properties":[{"key":"EntryName","value":"RocS-like_C"}]},{"database":"Pfam","id":"PF04394","properties":[{"key":"EntryName","value":"DUF536"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13412","properties":[{"key":"EntryName","value":"HTH_24"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVTRSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSEKDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARLFGG","length":163,"molWeight":18971,"crc64":"ACE8EFEEF8D2114D","md5":"E11E7EEFD22131AF9E150F06FA6B1D36"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Mutagenesis":1},"uniParcId":"UPI0000051689"}}