{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8CJM2","uniProtkbId":"PGLX_STRCO","entryAudit":{"firstPublicDate":"2021-04-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":102,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)","taxonId":100226,"lineage":["Bacteria","Bacillati","Actinomycetota","Actinomycetes","Kitasatosporales","Streptomycetaceae","Streptomyces","Streptomyces albidoflavus group"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"25592393"}],"value":"Adenine-specific methyltransferase PglX"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25592393"}],"value":"2.1.1.72"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11972785"}],"value":"Bacteriophage (PhiC31) resistance gene PglX"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12654995"}],"value":"Putative type II restriction enzyme and methyltransferase RM.ScoA3ORF6627P"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12654995"}],"value":"RM.ScoA3ORF6627P"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11972785"}],"value":"pglX"},"synonyms":[{"value":"SC1F2.24"}],"orderedLocusNames":[{"value":"SCO6627"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11972785"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12867465"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25592393"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"8446035"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"11972785"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25452498"}],"value":"BREX systems (bacteriophage exclusion) provide immunity against bacteriophage. Part of a type 2 BREX system (Probable). Probably a DNA methyltransferase, it methylates phage DNA in vitro in an S-adenosyl-L-methionine-dependent manner (PubMed:25592393). Previously called the phage growth limitation (Pgl) system, it confers protection against bacteriophage phiC31. The bacteria allows one cycle of phage infection, but subsequent cycles are impaired, protecting the original bacterial colony (Probable). The system undergoes high rates (10(-3) to 10(-4)) of phase reversion, i.e. loss and regain of phiC31 resistance (PubMed:12867465, PubMed:8446035). When the pglW-pglX-pglY-pglZ genes are transformed into a susceptible S.lividans (strain 1326) they confer resistance to infection by phage phiC31 and phiBT1; all 4 genes are necessary (PubMed:11972785)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25592393"}],"value":"Probably a toxic component of a type II toxin-antitoxin (TA) system. The toxic activity is inhibited by its cognate antitoxin PglZ"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12654995"}],"value":"May be a subtypes G and alpha restriction enzyme that recognizes and cleaves an unknown sequence. Methylates an adenine residue in the same sequence"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:15197"},{"database":"Rhea","id":"RHEA-COMP:12418"},{"database":"Rhea","id":"RHEA-COMP:12419"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:90615"},{"database":"ChEBI","id":"CHEBI:90616"}],"ecNumber":"2.1.1.72","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25592393"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11972785"}],"value":"Constitutively expressed, probably part of a pglW-pglX operon"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11972785"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25592393"}],"value":"Strain is no longer resistant to plaque formation by bacteriophage phiC31 (a Pgl- phenotype)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"12867465"}],"value":"Phase reversion of this Pgl system is due in part to the expansion and contraction of an 8-base long guanine tract in this protein (corresponding to residues 259-263). The tract has been observed to be 7, 8 and 9 nucleotides long in different derivatives; a 7-G tract yields a 282 residue and the 9-G tract a 336 residue missense protein"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the methyltransferase superfamily. PglX adenine methyltransferase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1210,"modifier":"EXACT"}},"description":"Adenine-specific methyltransferase PglX","featureId":"PRO_0000452165"},{"type":"Region","location":{"start":{"value":1181,"modifier":"EXACT"},"end":{"value":1210,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1181,"modifier":"EXACT"},"end":{"value":1194,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":1201,"modifier":"EXACT"},"end":{"value":1210,"modifier":"EXACT"}},"description":"Basic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Mutagenesis","location":{"start":{"value":381,"modifier":"EXACT"},"end":{"value":381,"modifier":"EXACT"}},"description":"Does not restore phage resistance to a deletion mutant, very little DNA methylation occurs in vitro.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25592393"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0051","category":"Biological process","name":"Antiviral defense"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"id":"KW-1277","category":"Biological process","name":"Toxin-antitoxin system"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"12000953","citationType":"journal article","authors":["Bentley S.D.","Chater K.F.","Cerdeno-Tarraga A.-M.","Challis G.L.","Thomson N.R.","James K.D.","Harris D.E.","Quail M.A.","Kieser H.","Harper D.","Bateman A.","Brown S.","Chandra G.","Chen C.W.","Collins M.","Cronin A.","Fraser A.","Goble A.","Hidalgo J.","Hornsby T.","Howarth S.","Huang C.-H.","Kieser T.","Larke L.","Murphy L.D.","Oliver K.","O'Neil S.","Rabbinowitsch E.","Rajandream M.A.","Rutherford K.M.","Rutter S.","Seeger K.","Saunders D.","Sharp S.","Squares R.","Squares S.","Taylor K.","Warren T.","Wietzorrek A.","Woodward J.R.","Barrell B.G.","Parkhill J.","Hopwood D.A."],"citationCrossReferences":[{"database":"PubMed","id":"12000953"},{"database":"DOI","id":"10.1038/417141a"}],"title":"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2).","publicationDate":"2002","journal":"Nature","firstPage":"141","lastPage":"147","volume":"417"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC BAA-471 / A3(2) / M145","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"8446035","citationType":"journal article","authors":["Laity C.","Chater K.F.","Lewis C.G.","Buttner M.J."],"citationCrossReferences":[{"database":"PubMed","id":"8446035"},{"database":"DOI","id":"10.1111/j.1365-2958.1993.tb01124.x"}],"title":"Genetic analysis of the phi C31-specific phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2).","publicationDate":"1993","journal":"Mol. Microbiol.","firstPage":"329","lastPage":"336","volume":"7"},"referencePositions":["GENETIC ANALYSIS","PHASE REVERSION"],"referenceComments":[{"value":"ATCC BAA-471 / A3(2) / M145","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"11972785","citationType":"journal article","authors":["Sumby P.","Smith M.C."],"citationCrossReferences":[{"database":"PubMed","id":"11972785"},{"database":"DOI","id":"10.1046/j.1365-2958.2002.02896.x"}],"title":"Genetics of the phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2).","publicationDate":"2002","journal":"Mol. Microbiol.","firstPage":"489","lastPage":"500","volume":"44"},"referencePositions":["FUNCTION IN ANTIVIRAL DEFENSE","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"ATCC BAA-471 / A3(2) / M145","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"12867465","citationType":"journal article","authors":["Sumby P.","Smith M.C."],"citationCrossReferences":[{"database":"PubMed","id":"12867465"},{"database":"DOI","id":"10.1128/jb.185.15.4558-4563.2003"}],"title":"Phase variation in the phage growth limitation system of Streptomyces coelicolor A3(2).","publicationDate":"2003","journal":"J. Bacteriol.","firstPage":"4558","lastPage":"4563","volume":"185"},"referencePositions":["INVOLVED IN PHASE VARIATION"],"referenceComments":[{"value":"ATCC BAA-471 / A3(2) / M145","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"12654995","citationType":"journal article","authors":["Roberts R.J.","Belfort M.","Bestor T.","Bhagwat A.S.","Bickle T.A.","Bitinaite J.","Blumenthal R.M.","Degtyarev S.K.","Dryden D.T.","Dybvig K.","Firman K.","Gromova E.S.","Gumport R.I.","Halford S.E.","Hattman S.","Heitman J.","Hornby D.P.","Janulaitis A.","Jeltsch A.","Josephsen J.","Kiss A.","Klaenhammer T.R.","Kobayashi I.","Kong H.","Krueger D.H.","Lacks S.","Marinus M.G.","Miyahara M.","Morgan R.D.","Murray N.E.","Nagaraja V.","Piekarowicz A.","Pingoud A.","Raleigh E.","Rao D.N.","Reich N.","Repin V.E.","Selker E.U.","Shaw P.C.","Stein D.C.","Stoddard B.L.","Szybalski W.","Trautner T.A.","Van Etten J.L.","Vitor J.M.","Wilson G.G.","Xu S.Y."],"citationCrossReferences":[{"database":"PubMed","id":"12654995"},{"database":"DOI","id":"10.1093/nar/gkg274"}],"title":"A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.","publicationDate":"2003","journal":"Nucleic Acids Res.","firstPage":"1805","lastPage":"1812","volume":"31"},"referencePositions":["NOMENCLATURE","SUBTYPE"]},{"referenceNumber":6,"citation":{"id":"25452498","citationType":"journal article","authors":["Goldfarb T.","Sberro H.","Weinstock E.","Cohen O.","Doron S.","Charpak-Amikam Y.","Afik S.","Ofir G.","Sorek R."],"citationCrossReferences":[{"database":"PubMed","id":"25452498"},{"database":"DOI","id":"10.15252/embj.201489455"}],"title":"BREX is a novel phage resistance system widespread in microbial genomes.","publicationDate":"2015","journal":"EMBO J.","firstPage":"169","lastPage":"183","volume":"34"},"referencePositions":["CLASSIFICATION AND NOMENCLATURE"]},{"referenceNumber":7,"citation":{"id":"25592393","citationType":"journal article","authors":["Hoskisson P.A.","Sumby P.","Smith M.C.M."],"citationCrossReferences":[{"database":"PubMed","id":"25592393"},{"database":"DOI","id":"10.1016/j.virol.2014.12.036"}],"title":"The phage growth limitation system in Streptomyces coelicolor A(3)2 is a toxin/antitoxin system, comprising enzymes with DNA methyltransferase, protein kinase and ATPase activity.","publicationDate":"2015","journal":"Virology","firstPage":"100","lastPage":"109","volume":"477"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE","MUTAGENESIS OF TYR-381"],"referenceComments":[{"value":"ATCC BAA-471 / A3(2) / M145","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AL939128","properties":[{"key":"ProteinId","value":"CAD55384.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"T29133","properties":[{"key":"EntryName","value":"T29133"}]},{"database":"PIR","id":"T35036","properties":[{"key":"EntryName","value":"T35036"}]},{"database":"RefSeq","id":"WP_011031053.1","properties":[{"key":"NucleotideSequenceId","value":"NC_003888.3"}]},{"database":"AlphaFoldDB","id":"Q8CJM2","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"100226.gene:17764285","properties":[{"key":"Description","value":"-"}]},{"database":"REBASE","id":"17310","properties":[{"key":"EntryName","value":"ScoA3ORF6627P"}]},{"database":"PaxDb","id":"100226-gene:17764285","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sco:SCO6627","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|100226.15.peg.6735","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG1002","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_269467_0_0_11","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8CJM2","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"4280289at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8CJM2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001973","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"Q8CJM2","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0003676","properties":[{"key":"GoTerm","value":"F:nucleic acid binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0009007","properties":[{"key":"GoTerm","value":"F:site-specific DNA-methyltransferase (adenine-specific) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-EC"}]},{"database":"GO","id":"GO:0006304","properties":[{"key":"GoTerm","value":"P:DNA modification"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"3.40.50.150","properties":[{"key":"EntryName","value":"Vaccinia Virus protein VP39"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002052","properties":[{"key":"EntryName","value":"DNA_methylase_N6_adenine_CS"}]},{"database":"InterPro","id":"IPR054277","properties":[{"key":"EntryName","value":"DUF7008"}]},{"database":"InterPro","id":"IPR011639","properties":[{"key":"EntryName","value":"MethylTrfase_TaqI-like_dom"}]},{"database":"InterPro","id":"IPR050953","properties":[{"key":"EntryName","value":"N4_N6_ade-DNA_methylase"}]},{"database":"InterPro","id":"IPR029063","properties":[{"key":"EntryName","value":"SAM-dependent_MTases_sf"}]},{"database":"NCBIfam","id":"NF033451","properties":[{"key":"EntryName","value":"BREX_2_MTaseX"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33841:SF1","properties":[{"key":"EntryName","value":"DNA METHYLTRANSFERASE A"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33841","properties":[{"key":"EntryName","value":"DNA METHYLTRANSFERASE YEEA-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22654","properties":[{"key":"EntryName","value":"DUF7008"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07669","properties":[{"key":"EntryName","value":"Eco57I"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53335","properties":[{"key":"EntryName","value":"S-adenosyl-L-methionine-dependent methyltransferases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00092","properties":[{"key":"EntryName","value":"N6_MTASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIDRKALLNDLKQQVKAVEADLGKQVKALDEVGARLRAEYDQARKLGRTAATWNSWLDERVTQVAVAWVLGTVFVRFCEDNRLIPEPYVTGPDNYRRDLAETRYDVYVEADDDPTYRGWLRRAFAELGDGQAGRLLFDSDHNPLYQIPLSHDGARELVEFWRQRDEEGALVHDFTDPLSADGTEGWGTRFLGDLYQDLSEAARKTYALLQTPEFVEEFILDRTMNPAVREFGYEELKMIDPTCGSGHFVLGAFRRLVRLGGENQPGKDVHQRVRAALDSVHGVDINPFAVAIARFRLLVAAMAASGVRTLDEASKYEWPVHLAVGDSLIKSGSQQGSLFGESDDDLTDELAEFKYATEDVGEHPEMLRPGRYHVVVGNPPYITVKDKSLNALYRELYPACAGKYALSVPFAQRFFELAKREDAEGSGYGMVGQITANSFMKREFGTKLIEGYFGHAVELTEVIDTSGAYIPGHGTPTVILVGTRRGGDGRSPVIRTVRSVQGEPVAPENAEEGLVWRAIVEQIDKPGSVSQWVSVDDLDREKYFSKQPWVLADGGQEMLEQINAASHAILKRDLHRIGFYGIMGADDAMSAVPRTFRRNNAESEYVRRLVVGDEVRDFRIADGDDAFHPYGSQRDLVGPDAFPNLAAWLWPYRTELGGRATFSGGTYFADGRPWWEWHQLPKDVGAHAWSLNFAFVATHNHVVLDRSGCAFTRTAPVIKLREGASEEEHLRLLGLLNSSTAGFWLKMVSYPKGGDPVGDEGARVSVHPWSDRYEFTGTKLQEFPLPSEYPTGLGTALDALAQRLAAASPAAVAAEAVPIAGLLREARTRWEAIRSRMIALQEEMDWQVYSLYKLHSEDLRVSEDPDDTNIPELTLGGRAFEIVLARRVAAGEASDEWFKRHNSTPITEIPAHWPAPYREIVQKRIDAIESNRAIGMVERPEYKRRWATEGWDALQEKALRSWLLDRMENRDLWCDENGQPTILTLARLTDALSRDEDFASVAKLYAPRKELAKVVAELITDEHVPFLSALRYKPSGLKKRADWEEVWDLQRKEDAAPDEPAKRKIRDSIPVPPKYTSADFLRPSYWKARGKLDVPKERFVSYGQTNAATPELYGWAGWDHREQAQALATYFTNTALSTKEITPFLAGLLELQPWLSQWHNEFDMLYSGSPADFFAGYRQQKQGEHGLTDDDLRGWRPPAATRRRRAAAKQ","length":1210,"molWeight":136254,"crc64":"9CBEEE2FFD5F6F90","md5":"B1D8D3B3D904E97AEA739CF9562C53E0"},"extraAttributes":{"countByCommentType":{"FUNCTION":3,"CATALYTIC ACTIVITY":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Compositional bias":2,"Mutagenesis":1},"uniParcId":"UPI00000E5837"}}