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Upon complete assembly of the IZUMO1 complex, its ER retention is released, facilitating IZUMO1 complex export to the acrosome (PubMed:35960805, PubMed:36050562). Through the interaction with SPPL2C, inhibits its intramembrane protease activity directly accessing the catalytic center of an I-CLiP (PubMed:35960805)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35960805"}],"value":"Interacts with SPPL2C (via active sites); the interaction stabilizes FREY1 protein and inhibits SPPL2C proteolytic activity (PubMed:35960805). Interacts with IZUMO1; the interaction retains IZUMO1 at the endoplasmic reticulum membrane and coordinates IZUMO1 complex assembly (PubMed:35960805)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35960805"}],"value":"Endoplasmic reticulum membrane","id":"SL-0097"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35960805"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36050562"}],"value":"Single-pass type II membrane protein","id":"SL-9906"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35960805"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36050562"}],"value":"Expressed in round spermatids (at protein level)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35960805"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36050562"}],"value":"Expressed in round spermatids belonging to stages V to VII/VIII with no visible expression in earlier or later stages"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35960805"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"36050562"}],"value":"Mutant males are normozoospermic infertile (PubMed:35960805, PubMed:36050562). 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