{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8C6I2","secondaryAccessions":["Q3TVE5","Q6PAS5","Q80ZJ9","Q8K2W8","Q9CYQ2"],"uniProtkbId":"SDHF2_MOUSE","entryAudit":{"firstPublicDate":"2007-07-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":138,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"Succinate dehydrogenase assembly factor 2, mitochondrial"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"SDH assembly factor 2"},{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"SDHAF2"}]},"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"Sdhaf2"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"Pgl2"},{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"Sdh5"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SDHA of the SDH catalytic dimer"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"Interacts with SDHA within the SDH catalytic dimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"Mitochondrion matrix","id":"SL-0170"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q8C6I2-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q8C6I2-2"],"sequenceIds":["VSP_026634"],"isoformSequenceStatus":"Described"},{"name":{"value":"3"},"isoformIds":["Q8C6I2-3"],"sequenceIds":["VSP_026633"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_03057"}],"value":"Belongs to the SDHAF2 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Mitochondrion","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":28,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"Succinate dehydrogenase assembly factor 2, mitochondrial","featureId":"PRO_0000294358"},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":94,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_026633","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":122,"modifier":"EXACT"},"end":{"value":164,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_026634","alternativeSequence":{"originalSequence":"EAKPAPEIFENEVMELLREFAKNKNKEQRLRAPDLEYLFEKPH","alternativeSequences":["GMPYAELHLGIIR"]}},{"type":"Sequence conflict","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"in Ref. 1; BAB28863","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["K"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0143","category":"Molecular function","name":"Chaperone"},{"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Head","type":"TISSUE"},{"value":"Kidney","type":"TISSUE"},{"value":"Stomach","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"FVB/N","type":"STRAIN"},{"value":"Brain","type":"TISSUE"},{"value":"Embryo","type":"TISSUE"},{"value":"Eye","type":"TISSUE"},{"value":"Mammary tumor","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Brown adipose tissue","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK160175","properties":[{"key":"ProteinId","value":"BAE35674.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK013454","properties":[{"key":"ProteinId","value":"BAB28863.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK075594","properties":[{"key":"ProteinId","value":"BAC35843.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK160957","properties":[{"key":"ProteinId","value":"BAE36113.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK167228","properties":[{"key":"ProteinId","value":"BAE39352.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC029630","properties":[{"key":"ProteinId","value":"AAH29630.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC038924","properties":[{"key":"ProteinId","value":"AAH38924.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC048913","properties":[{"key":"ProteinId","value":"AAH48913.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC060090","properties":[{"key":"ProteinId","value":"AAH60090.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC085277","properties":[{"key":"ProteinId","value":"AAH85277.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS29578.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8C6I2-1"},{"database":"RefSeq","id":"NP_079609.2","properties":[{"key":"NucleotideSequenceId","value":"NM_025333.4"}],"isoformId":"Q8C6I2-1"},{"database":"AlphaFoldDB","id":"Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"211194","properties":[{"key":"Interactions","value":"5"}]},{"database":"FunCoup","id":"Q8C6I2","properties":[{"key":"Number of interactors","value":"3121"}]},{"database":"STRING","id":"10090.ENSMUSP00000025570","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000025570","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"257108","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8C6I2-1"},{"database":"ProteomicsDB","id":"257109","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8C6I2-2"},{"database":"ProteomicsDB","id":"257110","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8C6I2-3"},{"database":"Pumba","id":"Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"51652","properties":[{"key":"antibodies","value":"57 antibodies from 24 providers"}]},{"database":"Ensembl","id":"ENSMUST00000025570.8","properties":[{"key":"ProteinId","value":"ENSMUSP00000025570.7"},{"key":"GeneId","value":"ENSMUSG00000024668.9"}],"isoformId":"Q8C6I2-1"},{"database":"Ensembl","id":"ENSMUST00000236455.2","properties":[{"key":"ProteinId","value":"ENSMUSP00000158166.2"},{"key":"GeneId","value":"ENSMUSG00000024668.9"}],"isoformId":"Q8C6I2-2"},{"database":"GeneID","id":"66072","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:66072","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc008gpt.2","properties":[{"key":"OrganismName","value":"mouse"}],"isoformId":"Q8C6I2-1"},{"database":"UCSC","id":"uc008gpv.1","properties":[{"key":"OrganismName","value":"mouse"}],"isoformId":"Q8C6I2-2"},{"database":"AGR","id":"MGI:1913322","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"54949","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1913322","properties":[{"key":"GeneName","value":"Sdhaf2"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000024668","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3326","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000001149","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_103054_0_2_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"YGKPQNP","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"14169at9989","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-MMU-9854311","properties":[{"key":"PathwayName","value":"Maturation of TCA enzymes and regulation of TCA cycle"}]},{"database":"BioGRID-ORCS","id":"66072","properties":[{"key":"hits","value":"17 hits in 79 CRISPR screens"}]},{"database":"PRO","id":"PR:Q8C6I2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 19"}]},{"database":"RNAct","id":"Q8C6I2","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000024668","properties":[{"key":"ExpressionPatterns","value":"Expressed in otic placode and 261 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q8C6I2","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005759","properties":[{"key":"GoTerm","value":"C:mitochondrial matrix"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"HDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"18614015"}]},{"database":"GO","id":"GO:0005730","properties":[{"key":"GoTerm","value":"C:nucleolus"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0006121","properties":[{"key":"GoTerm","value":"P:mitochondrial electron transport, succinate to ubiquinone"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0034553","properties":[{"key":"GoTerm","value":"P:mitochondrial respiratory chain complex II assembly"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0090090","properties":[{"key":"GoTerm","value":"P:negative regulation of canonical Wnt signaling pathway"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23983127"}]},{"database":"GO","id":"GO:0010719","properties":[{"key":"GoTerm","value":"P:negative regulation of epithelial to mesenchymal transition"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23983127"}]},{"database":"GO","id":"GO:0006470","properties":[{"key":"GoTerm","value":"P:protein dephosphorylation"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23983127"}]},{"database":"GO","id":"GO:0018293","properties":[{"key":"GoTerm","value":"P:protein-FAD linkage"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0006099","properties":[{"key":"GoTerm","value":"P:tricarboxylic acid cycle"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"FunFam","id":"1.10.150.250:FF:000002","properties":[{"key":"EntryName","value":"Succinate dehydrogenase assembly factor 2, mitochondrial"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.150.250","properties":[{"key":"EntryName","value":"Flavinator of succinate dehydrogenase"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_03057","properties":[{"key":"EntryName","value":"SDHAF2"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR005631","properties":[{"key":"EntryName","value":"SDH"}]},{"database":"InterPro","id":"IPR036714","properties":[{"key":"EntryName","value":"SDH_sf"}]},{"database":"InterPro","id":"IPR028882","properties":[{"key":"EntryName","value":"SDHAF2"}]},{"database":"PANTHER","id":"PTHR12469","properties":[{"key":"EntryName","value":"PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12469:SF2","properties":[{"key":"EntryName","value":"SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03937","properties":[{"key":"EntryName","value":"Sdh5"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF109910","properties":[{"key":"EntryName","value":"YgfY-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAVVTLIPTLARALSKHSLLSPLPSVTSFRRFYRGDSPTDSQKDMIEIPLPPWQERTDESIETKRARLLYESRKRGMLENCILLSLFAKEYLHNMTEKQLNLYDRLINEPSNDWDIYYWATEAKPAPEIFENEVMELLREFAKNKNKEQRLRAPDLEYLFEKPH","length":164,"molWeight":19431,"crc64":"CDC12D451D532BA4","md5":"CE0FABFB6076D1AE4D08198E60583174"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":3,"SIMILARITY":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Alternative sequence":2,"Sequence conflict":1},"uniParcId":"UPI00000223F0"}}