{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8C624","uniProtkbId":"SPT33_MOUSE","entryAudit":{"firstPublicDate":"2007-04-03","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2003-03-01","entryVersion":111,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Spermatogenesis-associated protein 33"}}},"genes":[{"geneName":{"value":"Spata33"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33087875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34446558"}],"value":"Plays an important role in sperm motility and male fertility (PubMed:34446558). Required for sperm midpiece flexibility and for the localization of sperm calcineurin to the mitochondria (PubMed:34446558). Promotes mitophagy as well as acts as an autophagy mediator in male germline cells (PubMed:33087875). Links damaged mitochondria to autophagosomes via its binding to the outer mitochondrial membrane protein VDAC2, as well as to key autophagy machinery component ATG16L1 (PubMed:33087875)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q96N06"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33087875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34446558"}],"value":"Interacts (via PQIIIT motif) with PPP3R2 and PPP3CC (PubMed:34446558). Interacts with VDAC2 (PubMed:33087875, PubMed:34446558). Interacts with ATG16L1 (via WD repeats) (PubMed:33087875). Interacts with PPP3R1, PPP3CA and PPP3CB (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23844118"}],"value":"Cytoplasm, cytosol","id":"SL-0091"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23844118"}],"value":"Nucleus","id":"SL-0191"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33087875"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33087875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34446558"}],"value":"Mitochondrion","id":"SL-0173"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23844118"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33087875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34446558"}],"value":"Predominantly expressed in the testis (at protein level) (PubMed:23844118, PubMed:33087875, PubMed:34446558). Expressed in the sperm midpiece (at protein level) (PubMed:33087875, PubMed:34446558)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23844118"}],"value":"Mainly expressed in the postpartum and adult testis. Predominantly expressed in the spermatocytes, as well as spermatogonia and round spermatids (at protein level). Expression increases during the first wave of the spermatogenesis"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33087875"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34446558"}],"value":"Mice exhibit reduced sperm motility because of an inflexible midpiece, leading to impaired male fertility (PubMed:34446558). Spata33 knockout in Sertoli cells and spermatogenic cells suppresses mitophagy (PubMed:33087875)"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"Spermatogenesis-associated protein 33","featureId":"PRO_0000282410"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"Interaction with ATG16L1","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33087875"}]},{"type":"Region","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"Interaction with VDAC2","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33087875"}]},{"type":"Region","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":79,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"PQIIIT","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"34446558"}]},{"type":"Compositional bias","location":{"start":{"value":24,"modifier":"EXACT"},"end":{"value":42,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]}],"keywords":[{"id":"KW-0072","category":"Biological process","name":"Autophagy"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"19468303","citationType":"journal article","authors":["Church D.M.","Goodstadt L.","Hillier L.W.","Zody M.C.","Goldstein S.","She X.","Bult C.J.","Agarwala R.","Cherry J.L.","DiCuccio M.","Hlavina W.","Kapustin Y.","Meric P.","Maglott D.","Birtle Z.","Marques A.C.","Graves T.","Zhou S.","Teague B.","Potamousis K.","Churas C.","Place M.","Herschleb J.","Runnheim R.","Forrest D.","Amos-Landgraf J.","Schwartz D.C.","Cheng Z.","Lindblad-Toh K.","Eichler E.E.","Ponting C.P."],"citationCrossReferences":[{"database":"PubMed","id":"19468303"},{"database":"DOI","id":"10.1371/journal.pbio.1000112"}],"title":"Lineage-specific biology revealed by a finished genome assembly of the mouse.","publicationDate":"2009","journal":"PLoS Biol.","firstPage":"E1000112","lastPage":"E1000112","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"23844118","citationType":"journal article","authors":["Chen H.","Yi M.","Sheng Y.","Cheng H.","Zhou R."],"citationCrossReferences":[{"database":"PubMed","id":"23844118"},{"database":"DOI","id":"10.1371/journal.pone.0067882"}],"title":"A novel testis-enriched gene Spata33 is expressed during spermatogenesis.","publicationDate":"2013","journal":"PLoS ONE","firstPage":"E67882","lastPage":"E67882","volume":"8"},"referencePositions":["SUBCELLULAR LOCATION","TISSUE SPECIFICITY","DEVELOPMENTAL STAGE"]},{"referenceNumber":5,"citation":{"id":"33087875","citationType":"journal article","authors":["Zhang Y.","Xu X.","Hu M.","Wang X.","Cheng H.","Zhou R."],"citationCrossReferences":[{"database":"PubMed","id":"33087875"},{"database":"DOI","id":"10.1038/s41418-020-00638-2"}],"title":"SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy.","publicationDate":"2021","journal":"Cell Death Differ.","firstPage":"1076","lastPage":"1090","volume":"28"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE","TISSUE SPECIFICITY","INTERACTION WITH ATG16L1 AND VDAC2"]},{"referenceNumber":6,"citation":{"id":"34446558","citationType":"journal article","authors":["Miyata H.","Oura S.","Morohoshi A.","Shimada K.","Mashiko D.","Oyama Y.","Kaneda Y.","Matsumura T.","Abbasi F.","Ikawa M."],"citationCrossReferences":[{"database":"PubMed","id":"34446558"},{"database":"DOI","id":"10.1073/pnas.2106673118"}],"title":"SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.","publicationDate":"2021","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"0","lastPage":"0","volume":"118"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE","TISSUE SPECIFICITY","INTERACTION WITH PPP3R2; PPP3CC AND VDAC2"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK076656","properties":[{"key":"ProteinId","value":"BAC36438.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"GL456146","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"CCDS","id":"CCDS40510.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_796253.2","properties":[{"key":"NucleotideSequenceId","value":"NM_177279.4"}]},{"database":"AlphaFoldDB","id":"Q8C624","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q8C624","properties":[{"key":"Number of interactors","value":"148"}]},{"database":"STRING","id":"10090.ENSMUSP00000058002","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q8C624","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q8C624","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000058002","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"263335","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"320869","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000060133.8","properties":[{"key":"ProteinId","value":"ENSMUSP00000058002.7"},{"key":"GeneId","value":"ENSMUSG00000048478.8"}]},{"database":"GeneID","id":"320869","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:320869","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc009nul.1","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:2444920","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"124045","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:2444920","properties":[{"key":"GeneName","value":"Spata33"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000048478","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502TF19","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT01140000286784","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_162385_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8C624","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"GPFYRHR","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10386166at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8C624","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"320869","properties":[{"key":"hits","value":"3 hits in 78 CRISPR screens"}]},{"database":"PRO","id":"PR:Q8C624","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 8"}]},{"database":"RNAct","id":"Q8C624","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000048478","properties":[{"key":"ExpressionPatterns","value":"Expressed in seminiferous tubule of testis and 93 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q8C624","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"33087875"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"34446558"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"23844118"}]},{"database":"GO","id":"GO:0097225","properties":[{"key":"GoTerm","value":"C:sperm midpiece"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"33087875"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"34446558"}]},{"database":"GO","id":"GO:0097226","properties":[{"key":"GoTerm","value":"C:sperm mitochondrial sheath"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"33087875"}]},{"database":"GO","id":"GO:0044877","properties":[{"key":"GoTerm","value":"F:protein-containing complex binding"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"34446558"}]},{"database":"GO","id":"GO:0009566","properties":[{"key":"GoTerm","value":"P:fertilization"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"34446558"}]},{"database":"GO","id":"GO:0030317","properties":[{"key":"GoTerm","value":"P:flagellated sperm motility"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"34446558"}]},{"database":"GO","id":"GO:0008104","properties":[{"key":"GoTerm","value":"P:intracellular protein localization"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"34446558"}]},{"database":"GO","id":"GO:0000423","properties":[{"key":"GoTerm","value":"P:mitophagy"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"33087875"}]},{"database":"InterPro","id":"IPR027930","properties":[{"key":"EntryName","value":"DUF4609"}]},{"database":"PANTHER","id":"PTHR38649","properties":[{"key":"EntryName","value":"SPERMATOGENESIS-ASSOCIATED PROTEIN 33"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR38649:SF1","properties":[{"key":"EntryName","value":"SPERMATOGENESIS-ASSOCIATED PROTEIN 33"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF15382","properties":[{"key":"EntryName","value":"DUF4609"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGQSKSKPREKKEEEKSTTTLVTKSKEKVMEKEAKQSDKESQPAESLLFATSKHSRPSSSSEDKPETKQRSSKKRSVIPQIIITRASNETLISYGIPDNDEQRTIREHADWGPYHRHRSPSTIAAYDVHNTE","length":132,"molWeight":15038,"crc64":"E37394D6D6ED4E2C","md5":"C3E03E2EBEA2B532E097320CE8715B3E"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DEVELOPMENTAL STAGE":1,"DISRUPTION PHENOTYPE":1},"countByFeatureType":{"Chain":1,"Region":4,"Motif":1,"Compositional bias":1,"Modified residue":1},"uniParcId":"UPI00000E9BC2"}}