{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8BVA5","secondaryAccessions":["A0A0F6AIX5","A0A1W2P7C8","Q3TRE7","Q5RL52","Q6PG15","Q8BVV0"],"uniProtkbId":"LDAH_MOUSE","entryAudit":{"firstPublicDate":"2007-09-11","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":134,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000000589"}],"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24357060"}],"value":"Lipid droplet-associated hydrolase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24357060"}],"value":"3.1.1.13"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24357060"}],"value":"Lipid droplet-associated serine hydrolase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24357060"}],"value":"mLDAH"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24357060"}],"value":"Ldah"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23525007"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24357060"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27836991"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"value":"Probable serine lipid hydrolase associated with lipid droplets (PubMed:24357060, PubMed:28578400). Has low cholesterol esterase activity (PubMed:24357060, PubMed:27836991). Appears to lack triglyceride lipase activity (PubMed:23525007, PubMed:24357060, PubMed:27836991). Involved in cholesterol and triglyceride homeostasis; stimulates cellular triglyceride accumulation and cellular cholesterol release (PubMed:24357060, PubMed:28578400). Acts antagonistically with PNPLA2/ATGL in regulation of cellular lipid stores (PubMed:28578400). May regulate triglyceride accumulation indirectly through stimulation of PNPLA2/ATGL ubiquitination and proteasomal degradation (PubMed:28578400). Promotes microtubule-dependent lipid droplet fusion (PubMed:28578400). Highly expressed in macrophage-rich areas in atherosclerotic lesions, suggesting that it could promote cholesterol ester turnover in macrophages (PubMed:24357060)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"value":"Stimulates cellular triglyceride accumulation and lipid droplet fusion"}],"commentType":"FUNCTION","molecule":"Isoform 1"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"value":"Associates with lipid droplets but does not stimulate cellular triglyceride accumulation, lipid droplet fusion or ATGL proteasomal degradation"}],"commentType":"FUNCTION","molecule":"Isoform 2"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"value":"Stimulates cellular triglyceride accumulation and lipid droplet fusion"}],"commentType":"FUNCTION","molecule":"Isoform 3"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a cholesterol ester + H2O = cholesterol + a fatty acid + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:36403"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:16113"},{"database":"ChEBI","id":"CHEBI:17002"},{"database":"ChEBI","id":"CHEBI:28868"}],"ecNumber":"3.1.1.13","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24357060"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:36404"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24357060"}]}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23525007"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"value":"Localizes to the endoplasmic reticulum in absence of lipid droplets and translocates to lipid droplets upon lipid storage induction (PubMed:23525007). Lipid droplet localization does not require hydrolase activity (PubMed:28578400)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23525007"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24357060"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"value":"Lipid droplet","id":"SL-0154"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23525007"}],"value":"Endoplasmic reticulum","id":"SL-0095"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28578400"}],"value":"1"},"isoformIds":["Q8BVA5-1"],"isoformSequenceStatus":"Displayed"},{"name":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28578400"}],"value":"2"},"isoformIds":["Q8BVA5-2"],"sequenceIds":["VSP_027797"],"isoformSequenceStatus":"Described"},{"name":{"value":"5"},"isoformIds":["Q8BVA5-3"],"sequenceIds":["VSP_027796"],"isoformSequenceStatus":"Described"},{"name":{"value":"4"},"isoformIds":["Q8BVA5-4"],"sequenceIds":["VSP_027796","VSP_027798","VSP_027799"],"isoformSequenceStatus":"Described"},{"name":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"28578400"}],"value":"3"},"isoformIds":["Q8BVA5-5"],"sequenceIds":["VSP_062355"],"isoformSequenceStatus":"Described"}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"value":"mRNAs for isoforms 1, 2 and 3 are present in differentiating adipocytes (PubMed:28578400). Isoform 1 is expressed at high levels throughout differentiation while isoforms 2 and 3 expression increases during differentiation but remain lower than isoform 1 levels (PubMed:28578400)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24357060"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27836991"}],"value":"Expressed in liver, adrenal gland, prostate, spleen, kidney, brown and white adipose tissue, testis and to a lesser extent in brain (at protein level) (PubMed:24357060, PubMed:27836991). Expressed in peritoneal macrophages and bone marrow-derived macrophages (at protein level) (PubMed:24357060, PubMed:27836991). Highly expressed in macrophage and foam cell-rich areas in atherosclerotic lesions (at protein level) (PubMed:24357060). mRNA, but no protein, expressed in heart and muscle (PubMed:24357060, PubMed:27836991)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27836991"}],"value":"Knockout mice have no obvious defects (PubMed:27836991). Size of adipocytes is normal (PubMed:27836991). Lipid metabolism is normal (PubMed:27836991). Cholesterol ester turnover or hydrolysis in bone marrow macrophages, brown adipose tissue or liver is normal (PubMed:27836991). No defect in body composition, energy expenditure, locomotor activity, water or food intake, respiratory exchange ratio, oxygen consumption, carbon dioxide production or glucose homeostasis (PubMed:27836991)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the AB hydrolase superfamily. LDAH family"}],"commentType":"SIMILARITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23525007"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24357060"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27836991"},{"evidenceCode":"ECO:0000305"}],"value":"The catalytic activity is unsure despite catalytic sites being conserved (PubMed:23525007, PubMed:27836991). One report shows that LDAH has low cholesterol esterase activity when overexpressed in RAW 264.7 macrophages or in HeLa cells (PubMed:24357060). However, in another study, LDAH lacks cholesterol esterase activity when overexpressed in Hela cells (PubMed:27836991). LDAH is not the only cellular cholesterol esterase and its activity may be too low to detect under certain experimental conditions. Lacks lipolytic activity towards trioleoylglycerol, dioleoylglycerol or monooleoylglycerol when overexpressed in COS-7 cells or Hela cells (PubMed:23525007, PubMed:27836991)"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":326,"modifier":"EXACT"}},"description":"Lipid droplet-associated hydrolase","featureId":"PRO_0000300125"},{"type":"Active site","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"Nucleophile","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10037"}]},{"type":"Active site","location":{"start":{"value":272,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H6V9"}]},{"type":"Active site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"Charge relay system","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H6V9"}]},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":170,"modifier":"EXACT"}},"description":"in isoform 5 and isoform 4","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16141072"}],"featureId":"VSP_027796","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":237,"modifier":"EXACT"},"end":{"value":326,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_027797","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":326,"modifier":"EXACT"}},"description":"in isoform 3","featureId":"VSP_062355","alternativeSequence":{"originalSequence":"LKFYYGKTDGWCPVKYYEDMKKDFPEGNIYLCEKGIPHAFVLDFSQEMATIVAEWINNRPPRK","alternativeSequences":["VTMGTFWGS"]}},{"type":"Alternative sequence","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"in isoform 4","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16141072"}],"featureId":"VSP_027798","alternativeSequence":{"originalSequence":"LKFYYGK","alternativeSequences":["EGNSTLP"]}},{"type":"Alternative sequence","location":{"start":{"value":271,"modifier":"EXACT"},"end":{"value":326,"modifier":"EXACT"}},"description":"in isoform 4","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16141072"}],"featureId":"VSP_027799","alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"No effect on cellular triglyceride accumulation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"Reduces cholesterol efflux but no effect on cellular triglyceride accumulation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"Abolishes active hydrolase probe binding in serine hydrolase assays. Abrogates cholesterol esterase activity and reduces cholesterol efflux. No effect on lipid droplet localization.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24357060"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["C"]}},{"type":"Mutagenesis","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"No effect on cellular triglyceride accumulation.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28578400"}],"alternativeSequence":{"originalSequence":"H","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":154,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"in Ref. 2; AAH46986/AAH57311 and 3","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["P"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0551","category":"Cellular component","name":"Lipid droplet"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 4 AND 5)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Diencephalon","type":"TISSUE"},{"value":"Head","type":"TISSUE"},{"value":"Hypothalamus","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"FVB/N","type":"STRAIN"},{"value":"Brain","type":"TISSUE"},{"value":"Colon","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"19468303","citationType":"journal article","authors":["Church D.M.","Goodstadt L.","Hillier L.W.","Zody M.C.","Goldstein S.","She X.","Bult C.J.","Agarwala R.","Cherry J.L.","DiCuccio M.","Hlavina W.","Kapustin Y.","Meric P.","Maglott D.","Birtle Z.","Marques A.C.","Graves T.","Zhou S.","Teague B.","Potamousis K.","Churas C.","Place M.","Herschleb J.","Runnheim R.","Forrest D.","Amos-Landgraf J.","Schwartz D.C.","Cheng Z.","Lindblad-Toh K.","Eichler E.E.","Ponting C.P."],"citationCrossReferences":[{"database":"PubMed","id":"19468303"},{"database":"DOI","id":"10.1371/journal.pbio.1000112"}],"title":"Lineage-specific biology revealed by a finished genome assembly of the mouse.","publicationDate":"2009","journal":"PLoS Biol.","firstPage":"E1000112","lastPage":"E1000112","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 3)"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000000589"}],"value":"C57BL/6J","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000000589"}]},{"referenceNumber":4,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Brain","type":"TISSUE"},{"value":"Brown adipose tissue","type":"TISSUE"},{"value":"Heart","type":"TISSUE"},{"value":"Liver","type":"TISSUE"},{"value":"Lung","type":"TISSUE"},{"value":"Pancreas","type":"TISSUE"},{"value":"Spleen","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"23525007","citationType":"journal article","authors":["Thiel K.","Heier C.","Haberl V.","Thul P.J.","Oberer M.","Lass A.","Jackle H.","Beller M."],"citationCrossReferences":[{"database":"PubMed","id":"23525007"},{"database":"DOI","id":"10.1242/jcs.120493"}],"title":"The evolutionarily conserved protein CG9186 is associated with lipid droplets, required for their positioning and for fat storage.","publicationDate":"2013","journal":"J. Cell Sci.","firstPage":"2198","lastPage":"2212","volume":"126"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":6,"citation":{"id":"24357060","citationType":"journal article","authors":["Goo Y.H.","Son S.H.","Kreienberg P.B.","Paul A."],"citationCrossReferences":[{"database":"PubMed","id":"24357060"},{"database":"DOI","id":"10.1161/atvbaha.113.302448"}],"title":"Novel lipid droplet-associated serine hydrolase regulates macrophage cholesterol mobilization.","publicationDate":"2014","journal":"Arterioscler. Thromb. Vasc. Biol.","firstPage":"386","lastPage":"396","volume":"34"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","IDENTIFICATION BY MASS SPECTROMETRY","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","MUTAGENESIS OF SER-140"]},{"referenceNumber":7,"citation":{"id":"27836991","citationType":"journal article","authors":["Kory N.","Grond S.","Kamat S.S.","Li Z.","Krahmer N.","Chitraju C.","Zhou P.","Froehlich F.","Semova I.","Ejsing C.","Zechner R.","Cravatt B.F.","Farese R.V. Jr.","Walther T.C."],"citationCrossReferences":[{"database":"PubMed","id":"27836991"},{"database":"DOI","id":"10.1194/jlr.m072538"}],"title":"Mice lacking lipid droplet-associated hydrolase, a gene linked to human prostate cancer, have normal cholesterol ester metabolism.","publicationDate":"2017","journal":"J. Lipid Res.","firstPage":"226","lastPage":"235","volume":"58"},"referencePositions":["FUNCTION","TISSUE SPECIFICITY","DISRUPTION PHENOTYPE"]},{"referenceNumber":8,"citation":{"id":"28578400","citationType":"journal article","authors":["Goo Y.H.","Son S.H.","Paul A."],"citationCrossReferences":[{"database":"PubMed","id":"28578400"},{"database":"DOI","id":"10.1038/s41598-017-02963-y"}],"title":"Lipid Droplet-Associated Hydrolase Promotes Lipid Droplet Fusion and Enhances ATGL Degradation and Triglyceride Accumulation.","publicationDate":"2017","journal":"Sci. Rep.","firstPage":"2743","lastPage":"2743","volume":"7"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","MUTAGENESIS OF HIS-127; SER-140 AND HIS-301"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK076460","properties":[{"key":"ProteinId","value":"BAC36352.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK079099","properties":[{"key":"ProteinId","value":"BAC37539.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK162850","properties":[{"key":"ProteinId","value":"BAE37083.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC046986","properties":[{"key":"ProteinId","value":"AAH46986.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC057311","properties":[{"key":"ProteinId","value":"AAH57311.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS25798.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BVA5-1"},{"database":"CCDS","id":"CCDS49023.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BVA5-2"},{"database":"CCDS","id":"CCDS88307.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BVA5-5"},{"database":"RefSeq","id":"NP_001161239.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001167767.2"}]},{"database":"RefSeq","id":"NP_765989.3","properties":[{"key":"NucleotideSequenceId","value":"NM_172401.5"}]},{"database":"AlphaFoldDB","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"213072","properties":[{"key":"Interactions","value":"2"}]},{"database":"FunCoup","id":"Q8BVA5","properties":[{"key":"Number of interactors","value":"963"}]},{"database":"STRING","id":"10090.ENSMUSP00000042285","properties":[{"key":"Description","value":"-"}]},{"database":"ESTHER","id":"mouse-Ldah","properties":[{"key":"FamilyName","value":"LIDHydrolase"}]},{"database":"iPTMnet","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000042285","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"264918","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BVA5-1"},{"database":"ProteomicsDB","id":"264919","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BVA5-2"},{"database":"ProteomicsDB","id":"264920","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BVA5-3"},{"database":"ProteomicsDB","id":"264921","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BVA5-4"},{"database":"ProteomicsDB","id":"326427","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"332892","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"27316","properties":[{"key":"antibodies","value":"145 antibodies from 18 providers"}]},{"database":"DNASU","id":"68832","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"68832","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:68832","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc007mzg.2","properties":[{"key":"OrganismName","value":"mouse"}],"isoformId":"Q8BVA5-1"},{"database":"UCSC","id":"uc007mzk.2","properties":[{"key":"OrganismName","value":"mouse"}],"isoformId":"Q8BVA5-3"},{"database":"AGR","id":"MGI:1916082","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"60526","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1916082","properties":[{"key":"GeneName","value":"Ldah"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000037669","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3975","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"WVPVSYY","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"448051at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"68832","properties":[{"key":"hits","value":"0 hits in 76 CRISPR screens"}]},{"database":"PRO","id":"PR:Q8BVA5","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 12"}]},{"database":"RNAct","id":"Q8BVA5","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000037669","properties":[{"key":"ExpressionPatterns","value":"Expressed in otic placode and 254 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q8BVA5","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23525007"}]},{"database":"GO","id":"GO:0005811","properties":[{"key":"GoTerm","value":"C:lipid droplet"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"24357060"}]},{"database":"GO","id":"GO:0004771","properties":[{"key":"GoTerm","value":"F:sterol ester esterase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"24357060"}]},{"database":"GO","id":"GO:0042632","properties":[{"key":"GoTerm","value":"P:cholesterol homeostasis"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"24357060"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"28578400"}]},{"database":"GO","id":"GO:0090077","properties":[{"key":"GoTerm","value":"P:foam cell differentiation"},{"key":"GoEvidenceType","value":"TAS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000304","source":"PubMed","id":"24357060"}]},{"database":"GO","id":"GO:0035356","properties":[{"key":"GoTerm","value":"P:intracellular triglyceride homeostasis"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"28578400"}]},{"database":"GO","id":"GO:0160077","properties":[{"key":"GoTerm","value":"P:lipid droplet fusion"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"28578400"}]},{"database":"GO","id":"GO:0034389","properties":[{"key":"GoTerm","value":"P:lipid droplet organization"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0019915","properties":[{"key":"GoTerm","value":"P:lipid storage"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"FunFam","id":"3.40.50.1820:FF:000068","properties":[{"key":"EntryName","value":"Lipid droplet associated hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.1820","properties":[{"key":"EntryName","value":"alpha/beta hydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR029058","properties":[{"key":"EntryName","value":"AB_hydrolase_fold"}]},{"database":"InterPro","id":"IPR019363","properties":[{"key":"EntryName","value":"LDAH"}]},{"database":"PANTHER","id":"PTHR13390","properties":[{"key":"EntryName","value":"LIPASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR13390:SF0","properties":[{"key":"EntryName","value":"LIPID DROPLET-ASSOCIATED HYDROLASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10230","properties":[{"key":"EntryName","value":"LIDHydrolase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF53474","properties":[{"key":"EntryName","value":"alpha/beta-Hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00120","properties":[{"key":"EntryName","value":"LIPASE_SER"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MASEVEEQIPVREEFFLCGGVETKIIKCGPWTNLFEKQDVSKPKQLIFIIPGNPGYSAFYVPFAKALYTLMKSRFPVWIISHAGFSVTPKDKKVLAAPQEESNAQKIEDVYGLNGQIEHKIAFLRAHVPKDVKLILIGHSVGTYMTLHVMKRVLELPVAHAFLLFPTIERMSESPNGKFATPFLCQFRYLLYATSYLLFKPCPEVIKSFIIQKLMGQMNIKLELPLTDILQPFCLANAAYLGSQEMVQIVKRDDDIIKEFLPKLKFYYGKTDGWCPVKYYEDMKKDFPEGNIYLCEKGIPHAFVLDFSQEMATIVAEWINNRPPRK","length":326,"molWeight":37374,"crc64":"EE979E9FB9FA9C28","md5":"F21E5E57DFAD1F41A567EB10FDEC83E6"},"extraAttributes":{"countByCommentType":{"FUNCTION":4,"CATALYTIC ACTIVITY":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":5,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1,"CAUTION":1},"countByFeatureType":{"Chain":1,"Active site":3,"Alternative sequence":5,"Mutagenesis":4,"Sequence conflict":1},"uniParcId":"UPI00000EA934"}}