{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q8BGR8","secondaryAccessions":["Q8CDW3","Q8K2G9"],"uniProtkbId":"GSKIP_MOUSE","entryAudit":{"firstPublicDate":"2003-09-09","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-03-01","entryVersion":127,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}],"value":"GSK3B-interacting protein"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}],"value":"GSKIP"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:1914037"}],"value":"Gskip"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26582204"}],"value":"A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets. The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively. Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B. During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression (By similarity). May play a role in cleft palate formation and is required for postnatal life through modulation of the activity of GSK3B during development (PubMed:26582204)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}],"value":"Forms a complex composed of PRKAR2A or PRKAR2B, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation of GSK3B leading to GSK3B inactivation; recruits DNM1L through GSK3B for PKA-mediated phosphorylation of DNM1L; promotes beta-catenin degradation through GSK3B-induced phosphorylation of beta-catenin; stabilizes beta-catenin and enhances Wnt-induced signaling through PKA-induced phosphorylation of beta-catenin. Interacts with GSK3B; induces GSK3B-mediated phosphorylation of GSKIP and inhibits GSK3B kinase activity"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}],"value":"Nucleus","id":"SL-0191"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q8BGR8-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q8BGR8-2"],"sequenceIds":["VSP_008199"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}],"value":"Phosphorylated by GSK3B"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26582204"}],"value":"Knockout Gskip mice die at birth. At 18.5 dpc, embryos are still alive but rapidly die within 5 to 30 minutes after casarean section. Embryos obtained at 18.5 dpc are cyanotic, suffer from respiratory distress, and fail to initiate breathing properly"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the GSKIP family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"GSK3B-interacting protein","featureId":"PRO_0000220952"},{"type":"Region","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"Required for PRKAR2A interaction; contributes to a protective effect against H(2)O(2)-induced apoptosis","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}]},{"type":"Region","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":139,"modifier":"EXACT"}},"description":"Interaction with GSK3B and acts as a GSK3B inhibitor","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}]},{"type":"Site","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":130,"modifier":"EXACT"}},"description":"Required for GSK3B interaction; contributes to a protective effect against H(2)O(2)-induced apoptosis","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9P0R6"}]},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_008199","alternativeSequence":{"originalSequence":"M","alternativeSequences":["MGARRM"]}},{"type":"Sequence conflict","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":6,"modifier":"EXACT"}},"description":"in Ref. 1; BAC26470","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"N","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Cerebellum","type":"TISSUE"},{"value":"Head","type":"TISSUE"},{"value":"Thymus","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)"],"referenceComments":[{"value":"Mammary tumor","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"26582204","citationType":"journal article","authors":["Deak V.A.","Skroblin P.","Dittmayer C.","Knobeloch K.P.","Bachmann S.","Klussmann E."],"citationCrossReferences":[{"database":"PubMed","id":"26582204"},{"database":"DOI","id":"10.1074/jbc.m115.701177"}],"title":"The A-kinase Anchoring Protein GSKIP Regulates GSK3beta Activity and Controls Palatal Shelf Fusion in Mice.","publicationDate":"2016","journal":"J. Biol. Chem.","firstPage":"681","lastPage":"690","volume":"291"},"referencePositions":["DISRUPTION PHENOTYPE","FUNCTION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK029484","properties":[{"key":"ProteinId","value":"BAC26470.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK042664","properties":[{"key":"ProteinId","value":"BAC31325.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK079775","properties":[{"key":"ProteinId","value":"BAC37748.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC031494","properties":[{"key":"ProteinId","value":"AAH31494.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS49161.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BGR8-2"},{"database":"RefSeq","id":"NP_848728.2","properties":[{"key":"NucleotideSequenceId","value":"NM_178613.3"}],"isoformId":"Q8BGR8-2"},{"database":"AlphaFoldDB","id":"Q8BGR8","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q8BGR8","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"211719","properties":[{"key":"Interactions","value":"9"}]},{"database":"FunCoup","id":"Q8BGR8","properties":[{"key":"Number of interactors","value":"3033"}]},{"database":"STRING","id":"10090.ENSMUSP00000057939","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000057939","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"271055","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BGR8-1"},{"database":"ProteomicsDB","id":"271056","properties":[{"key":"Description","value":"-"}],"isoformId":"Q8BGR8-2"},{"database":"Pumba","id":"Q8BGR8","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"27335","properties":[{"key":"antibodies","value":"83 antibodies from 17 providers"}]},{"database":"DNASU","id":"66787","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000051934.7","properties":[{"key":"ProteinId","value":"ENSMUSP00000057939.6"},{"key":"GeneId","value":"ENSMUSG00000044715.8"}],"isoformId":"Q8BGR8-2"},{"database":"GeneID","id":"66787","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:66787","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"MGI:1914037","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"51527","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1914037","properties":[{"key":"GeneName","value":"Gskip"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000044715","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3965","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000009517","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_143747_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q8BGR8","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"FAVTEMH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"5804279at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"66787","properties":[{"key":"hits","value":"5 hits in 76 CRISPR screens"}]},{"database":"PRO","id":"PR:Q8BGR8","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 12"}]},{"database":"RNAct","id":"Q8BGR8","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000044715","properties":[{"key":"ExpressionPatterns","value":"Expressed in placenta labyrinth and 222 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q8BGR8","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0048471","properties":[{"key":"GoTerm","value":"C:perinuclear region of cytoplasm"},{"key":"GoEvidenceType","value":"ISS:BHF-UCL"}]},{"database":"GO","id":"GO:0019207","properties":[{"key":"GoTerm","value":"F:kinase regulator activity"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0051018","properties":[{"key":"GoTerm","value":"F:protein kinase A binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0034237","properties":[{"key":"GoTerm","value":"F:protein kinase A regulatory subunit binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0019901","properties":[{"key":"GoTerm","value":"F:protein kinase binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0004860","properties":[{"key":"GoTerm","value":"F:protein kinase inhibitor activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0008631","properties":[{"key":"GoTerm","value":"P:intrinsic apoptotic signaling pathway in response to oxidative stress"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0006469","properties":[{"key":"GoTerm","value":"P:negative regulation of protein kinase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0090263","properties":[{"key":"GoTerm","value":"P:positive regulation of canonical Wnt signaling pathway"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0030111","properties":[{"key":"GoTerm","value":"P:regulation of Wnt signaling pathway"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"FunFam","id":"3.30.2280.10:FF:000003","properties":[{"key":"EntryName","value":"GSK3-beta interaction protein"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.2280.10","properties":[{"key":"EntryName","value":"Hypothetical protein (hspc210)"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR037395","properties":[{"key":"EntryName","value":"GSKIP"}]},{"database":"InterPro","id":"IPR007967","properties":[{"key":"EntryName","value":"GSKIP_dom"}]},{"database":"InterPro","id":"IPR023231","properties":[{"key":"EntryName","value":"GSKIP_dom_sf"}]},{"database":"PANTHER","id":"PTHR12490","properties":[{"key":"EntryName","value":"GSK3B-INTERACTING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12490:SF4","properties":[{"key":"EntryName","value":"GSK3B-INTERACTING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05303","properties":[{"key":"EntryName","value":"GSKIP_dom"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF103107","properties":[{"key":"EntryName","value":"Hypothetical protein c14orf129, hspc210"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"METDYNPVELSSMSGFEEGSELNGFEGADMKDMQLEAEAVVNDVLFAVNHMFVSKSMPCADDVAYINVETKERNRYCLELTEAGLRVVGYAFDQVEDHLQTPYHETVYSLLDTLSPAYREAFGNALLQRLEALKRDGQS","length":139,"molWeight":15642,"crc64":"78082F4AC59F7ECE","md5":"5F4A1B7ECF07B1EF67EDA438C3C1B5DE"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"PTM":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":2,"Site":1,"Alternative sequence":1,"Sequence conflict":1},"uniParcId":"UPI00000EB9C4"}}