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Contributes to intrinsic fosfomycin resistance in P.putida (PubMed:23831760)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"1,6-anhydro-N-acetyl-beta-muramate + ATP + H2O = N-acetyl-D-muramate 6-phosphate + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:24952"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:58690"},{"database":"ChEBI","id":"CHEBI:58722"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.1.170","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01270"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01270"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23831760"}],"value":"Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01270"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23831760"}],"value":"Cell wall biogenesis; peptidoglycan recycling"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23831760"}],"value":"Cells lacking this gene accumulate anhMurNAc and are fosfomycin sensitive, in contrast to wild-type P.putida, which is resistant to fosfomycin"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01270"}],"value":"Belongs to the anhydro-N-acetylmuramic acid kinase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":363,"modifier":"EXACT"}},"description":"Anhydro-N-acetylmuramic acid kinase","featureId":"PRO_0000250031"},{"type":"Binding site","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":17,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01270"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}}],"keywords":[{"id":"KW-0046","category":"Biological process","name":"Antibiotic resistance"},{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0119","category":"Biological process","name":"Carbohydrate metabolism"},{"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"12534463","citationType":"journal article","authors":["Nelson K.E.","Weinel C.","Paulsen I.T.","Dodson R.J.","Hilbert H.","Martins dos Santos V.A.P.","Fouts D.E.","Gill S.R.","Pop M.","Holmes M.","Brinkac L.M.","Beanan M.J.","DeBoy R.T.","Daugherty S.C.","Kolonay J.F.","Madupu R.","Nelson W.C.","White O.","Peterson J.D.","Khouri H.M.","Hance I.","Chris Lee P.","Holtzapple E.K.","Scanlan D.","Tran K.","Moazzez A.","Utterback T.R.","Rizzo M.","Lee K.","Kosack D.","Moestl D.","Wedler H.","Lauber J.","Stjepandic D.","Hoheisel J.","Straetz M.","Heim S.","Kiewitz C.","Eisen J.A.","Timmis K.N.","Duesterhoeft A.","Tuemmler B.","Fraser C.M."],"citationCrossReferences":[{"database":"PubMed","id":"12534463"},{"database":"DOI","id":"10.1046/j.1462-2920.2002.00366.x"}],"title":"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.","publicationDate":"2002","journal":"Environ. 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