{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q834S9","uniProtkbId":"REOY_ENTFA","entryAudit":{"firstPublicDate":"2004-04-13","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-06-01","entryVersion":84,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Enterococcus faecalis (strain ATCC 700802 / V583)","taxonId":226185,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Lactobacillales","Enterococcaceae","Enterococcus"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8Y5Y2"}],"value":"Peptidoglycan synthesis regulatory protein ReoY"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8Y5Y2"}],"value":"Regulator of MurA(A) degradation ReoY"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"29038125"}],"value":"reoY"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12663927"}],"value":"EF_1554"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8Y5Y2"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29038125"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37688380"}],"value":"Involved in the PASTA kinase-mediated signaling pathway regulating peptidoglycan synthesis to maintain cell wall integrity (By similarity). Modulates peptidoglycan (PG) synthesis pathway committed-step enzyme MurAA (PubMed:37688380). Positively modulates ClpCP-dependent proteolysis of MurAA, thereby regulating PG synthesis and inhibiting intrinsic cephalosporin resistance (PubMed:37688380). May antagonize IreK kinase, perhaps acting in concert with TPR domain protein EF_1553, playing a downstream role in modulating cell wall integrity, antimicrobial resistance and colonization of the mammalian gastrointestinal tract (PubMed:29038125)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8Y5Y2"}],"value":"Interacts directly with IreB/ReoM"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"29038125"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29038125"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"29038125"}],"value":"Expression is not IreK- or IreP-dependent (at mRNA level) (PubMed:29038125). Expression is unaffected by lysozyme treatment (at mRNA level) (PubMed:29038125). Probably part of an operon with EF_1552 and EF_1553 (Probable)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29038125"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37688380"}],"value":"Increases MurAA protein levels, by comparison with the OG1RF strain (PubMed:37688380). Does not alter ceftriaxone resistance by comparison with wild-type OG1RF strain, but partially suppresses sensitivity to ceftriaxone in an ireK mutant background (PubMed:37688380). Does not alter cholate or lysozyme resistance by comparison with wild-type OG1RF strain, but suppresses sensitivity to cholate and lysozyme in an ireK mutant background (PubMed:29038125). Returns cell envelope integrity almost to wild-type, as measured by permeability to chlorophenol red beta-D-galactoside (CPRG), in an ireK mutant background in OG1RF strain (PubMed:29038125). Suppresses the mouse gastrointestinal tract colonization defect of the ireK mutant background in OG1RF strain (PubMed:29038125). Expression and phosphorylation of IreB unaffected in the ireK mutant background in OG1RF strain (PubMed:29038125)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00760"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the ReoY family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"Peptidoglycan synthesis regulatory protein ReoY","featureId":"PRO_0000216099"}],"keywords":[{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"12663927","citationType":"journal article","authors":["Paulsen I.T.","Banerjei L.","Myers G.S.A.","Nelson K.E.","Seshadri R.","Read T.D.","Fouts D.E.","Eisen J.A.","Gill S.R.","Heidelberg J.F.","Tettelin H.","Dodson R.J.","Umayam L.A.","Brinkac L.M.","Beanan M.J.","Daugherty S.C.","DeBoy R.T.","Durkin S.A.","Kolonay J.F.","Madupu R.","Nelson W.C.","Vamathevan J.J.","Tran B.","Upton J.","Hansen T.","Shetty J.","Khouri H.M.","Utterback T.R.","Radune D.","Ketchum K.A.","Dougherty B.A.","Fraser C.M."],"citationCrossReferences":[{"database":"PubMed","id":"12663927"},{"database":"DOI","id":"10.1126/science.1080613"}],"title":"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis.","publicationDate":"2003","journal":"Science","firstPage":"2071","lastPage":"2074","volume":"299"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 700802 / V583","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"29038125","citationType":"journal article","authors":["Banla I.L.","Kommineni S.","Hayward M.","Rodrigues M.","Palmer K.L.","Salzman N.H.","Kristich C.J."],"citationCrossReferences":[{"database":"PubMed","id":"29038125"},{"database":"DOI","id":"10.1128/iai.00381-17"}],"title":"Modulators of Enterococcus faecalis Cell Envelope Integrity and Antimicrobial Resistance Influence Stable Colonization of the Mammalian Gastrointestinal Tract.","publicationDate":"2018","journal":"Infect. Immun.","firstPage":"0","lastPage":"0","volume":"86"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","INDUCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29038125"}],"value":"OG1RF","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000305"}]},{"referenceNumber":3,"citation":{"id":"37688380","citationType":"journal article","authors":["Mascari C.A.","Little J.L.","Kristich C.J."],"citationCrossReferences":[{"database":"PubMed","id":"37688380"},{"database":"DOI","id":"10.1111/mmi.15150"}],"title":"PASTA-kinase-mediated signaling drives accumulation of the peptidoglycan synthesis protein MurAA to promote cephalosporin resistance in Enterococcus faecalis.","publicationDate":"2023","journal":"Mol. Microbiol.","firstPage":"811","lastPage":"829","volume":"120"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"],"referenceComments":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37688380"}],"value":"OG1","type":"STRAIN"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37688380"}],"value":"OG1RF","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AE016830","properties":[{"key":"ProteinId","value":"AAO81341.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_002382318.1","properties":[{"key":"NucleotideSequenceId","value":"NC_004668.1"}]},{"database":"AlphaFoldDB","id":"Q834S9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q834S9","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"226185.EF_1554","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"226185-EF_1554","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAO81341","properties":[{"key":"ProteinId","value":"AAO81341"},{"key":"GeneId","value":"EF_1554"}]},{"database":"KEGG","id":"efa:EF1554","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|226185.45.peg.1951","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"COG5582","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_126019_1_0_9","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000001415","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"Gene3D","id":"3.40.1530.30","properties":[{"key":"EntryName","value":"Uncharacterised family UPF0302, N-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"4.10.810.10","properties":[{"key":"EntryName","value":"Virus Scaffolding Protein, Chain A"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00760","properties":[{"key":"EntryName","value":"ReoY"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR014957","properties":[{"key":"EntryName","value":"IDEAL_dom"}]},{"database":"InterPro","id":"IPR011188","properties":[{"key":"EntryName","value":"ReoY-like"}]},{"database":"InterPro","id":"IPR014963","properties":[{"key":"EntryName","value":"ReoY-like_N"}]},{"database":"InterPro","id":"IPR038091","properties":[{"key":"EntryName","value":"ReoY-like_N_sf"}]},{"database":"InterPro","id":"IPR027393","properties":[{"key":"EntryName","value":"Virus_scaffolding_prot_C"}]},{"database":"NCBIfam","id":"NF002965","properties":[{"key":"EntryName","value":"PRK03636.1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08858","properties":[{"key":"EntryName","value":"IDEAL"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08864","properties":[{"key":"EntryName","value":"ReoY_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF007165","properties":[{"key":"EntryName","value":"UCP007165"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00914","properties":[{"key":"EntryName","value":"IDEAL"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MFVSLSEKKKFLTWLVNTAPFGRREVLWILNYLLTHDAILNNVHFVENVEKTDRGIRVVADGLGKEPLLLFIQAQEFTDPEQIFHEIRMNWRKALYLECVFPEAWQTSQYLSVLEDNPFAPWNEQVDQEVARAIDQYFKQEEQTQRMALLKAQIDDALETGNKEAFLELSDELNRLKQQ","length":179,"molWeight":21089,"crc64":"36E8E1AB4F254030","md5":"059E461996EFD0ACBE788CE003F278C4"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI000005C221"}}