{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q834B6","uniProtkbId":"LIAX_ENTFA","entryAudit":{"firstPublicDate":"2024-01-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-06-01","entryVersion":97,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Enterococcus faecalis (strain ATCC 700802 / V583)","taxonId":226185,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001415"}],"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Lactobacillales","Enterococcaceae","Enterococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Putative adhesin domain-containing protein LiaX"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Daptomycin-sensing surface protein LiaX"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31818937"}],"value":"liaX"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"31818937"},{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAO81526.1"}],"value":"EF_1753"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"Involved in cell membrane remodeling, perhaps acting by negative modulation of the liaFSR and liaXYZ gene clusters, thereby regulating content and localization of anionic phospholipids (PubMed:31818937). Binds to the antibiotic daptomycin (DAP) and to cationic antimicrobial peptides, such as human LL-37, perhaps functioning as a sensor that activates the cell envelope stress response (PubMed:31818937)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"Despite lack of predicted classical secretion signals, detected in the extracellular environment"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"Secreted, cell wall","id":"SL-0041"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"Cell membrane","id":"SL-0039"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"Secreted","id":"SL-0243"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"Probably part of a liaX-liaY-liaZ putative operon"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"The N-terminal region (1-277) is able to bind the antibiotic daptomycin (DAP) and the antimicrobial peptide human LL-37, under physiologically relevant concentrations"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"The C-terminal region (278-533) is involved in cell membrane remodeling"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"May undergo proteolytic cleavage, allowing release of the N-terminal region into the extracellular environment"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"Deletion in the OG1RF strain causes anionic phospholipids to be redistributed away from the division septum; during exponential growth, there are increases in phosphatidylglycerol (PG), decreases in cardiolipin, with no change in lysyl-PG (PubMed:31818937). Confers resistance to the antibiotic daptomycin (DAP) in the OG1RF strain (PubMed:31818937). Resistant to killing of the OG1RF strain by human cathelicidin LL-37 (PubMed:31818937). Deletion in the OG1RF strain increases virulence in nematode C.elegans infection model (PubMed:31818937)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}],"value":"LiaX and its N-terminal region are released into the extracellular environment in daptomycin (DAP) resistant strains, such as R712"}],"commentType":"MISCELLANEOUS"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":533,"modifier":"EXACT"}},"description":"Putative adhesin domain-containing protein LiaX","featureId":"PRO_0000459239"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":277,"modifier":"EXACT"}},"description":"Binds the antibiotic daptomycin (DAP) and the antimicrobial peptide human LL-37, under physiologically relevant concentrations. Protects the OG1RF and S613 strains from LL-37-mediated killing in a concentration-dependent manner","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}]},{"type":"Region","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":279,"modifier":"EXACT"},"end":{"value":526,"modifier":"EXACT"}},"description":"Putative adhesin region","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Region","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":526,"modifier":"EXACT"}},"description":"Involved in cell membrane remodeling","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31818937"}]},{"type":"Coiled coil","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":186,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Mutagenesis","location":{"start":{"value":152,"modifier":"EXACT"},"end":{"value":533,"modifier":"EXACT"}},"description":"Confers antibiotic daptomycin (DAP)-resistance in clinical isolate. 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