{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q81RQ5","secondaryAccessions":["E9R8M9","Q6KTW1"],"uniProtkbId":"ASBE_BACAN","entryAudit":{"firstPublicDate":"2020-08-12","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-06-01","entryVersion":117,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Bacillus anthracis","taxonId":1392,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Bacillus","Bacillus cereus group"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Petrobactin synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22408253"}],"value":"2.3.2.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Petrobactin biosynthesis protein AsbE"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17189355"}],"value":"asbE"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAT31104.1"}],"value":"GBAA_1985"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17189355"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17346033"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22408253"}],"value":"Involved in the biosynthesis of petrobactin, a catecholate siderophore that functions in both iron acquisition and virulence (PubMed:17189355, PubMed:17346033, PubMed:22408253). Transfers the activated 3,4-dihydroxybenzoate (3,4-DHBA) moiety from 3,4-DHBA-loaded AsbD to different receipient molecules, including N-citryl-spermidine, N8,N'8-citryl-bis(spermidine) and N1-(3,4-dihydroxybenzoyl)-N8,N'8-citryl-bis(spermidine) (PubMed:22408253). Also catalyzes the transfer of the activated 3,4-DHBA moiety from 3,4-DHBA-loaded AsbD to spermidine to generate DHB-spermidine (DHB-SP) (PubMed:17346033)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N(8)-citryl-spermidine + 3,4-dihydroxybenzoyl-[aryl-carrier protein] = N(1)-(3,4-dihydroxybenzoyl)-N(8)-citryl-spermidine + holo-[aryl-carrier protein] + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:64028"},{"database":"Rhea","id":"RHEA-COMP:15903"},{"database":"Rhea","id":"RHEA-COMP:15941"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:144963"},{"database":"ChEBI","id":"CHEBI:149586"},{"database":"ChEBI","id":"CHEBI:149593"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22408253"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N(8),N'(8)-citryl-bis(spermidine) + 3,4-dihydroxybenzoyl-[aryl-carrier protein] = N(1)-(3,4-dihydroxybenzoyl)-N(8),N'(8)-citryl-bis(spermidine) + holo-[aryl-carrier protein] + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:64024"},{"database":"Rhea","id":"RHEA-COMP:15903"},{"database":"Rhea","id":"RHEA-COMP:15941"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:144963"},{"database":"ChEBI","id":"CHEBI:149592"},{"database":"ChEBI","id":"CHEBI:149594"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22408253"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"N(1)-(3,4-dihydroxybenzoyl)-N(8),N'(8)-citryl-bis(spermidine) + 3,4-dihydroxybenzoyl-[aryl-carrier protein] = petrobactin + holo-[aryl-carrier protein] + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:63820"},{"database":"Rhea","id":"RHEA-COMP:15903"},{"database":"Rhea","id":"RHEA-COMP:15941"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:64479"},{"database":"ChEBI","id":"CHEBI:142778"},{"database":"ChEBI","id":"CHEBI:144963"},{"database":"ChEBI","id":"CHEBI:149594"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22408253"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17189355"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17346033"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22408253"}],"value":"Siderophore biosynthesis; petrobactin biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17189355"}],"value":"The deletion mutant cannot produce petrobactin on iron-depleted medium. In vitro analysis show that mutants grow to a very limited extent as vegetative cells in iron-depleted medium but are not able to outgrow from spores under the same culture conditions"}],"commentType":"DISRUPTION PHENOTYPE"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":327,"modifier":"EXACT"}},"description":"Petrobactin synthase","featureId":"PRO_0000450625"}],"keywords":[{"id":"KW-0012","category":"Molecular function","name":"Acyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"18952800","citationType":"journal article","authors":["Ravel J.","Jiang L.","Stanley S.T.","Wilson M.R.","Decker R.S.","Read T.D.","Worsham P.","Keim P.S.","Salzberg S.L.","Fraser-Liggett C.M.","Rasko D.A."],"citationCrossReferences":[{"database":"PubMed","id":"18952800"},{"database":"DOI","id":"10.1128/jb.01347-08"}],"title":"The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.","publicationDate":"2009","journal":"J. 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