{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q80HV6","uniProtkbId":"PG141_VACCW","entryAudit":{"firstPublicDate":"2011-12-14","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-06-01","entryVersion":51,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Vaccinia virus (strain Western Reserve)","commonName":"VACV","synonyms":["Vaccinia virus (strain WR)"],"taxonId":10254,"lineage":["Viruses","Varidnaviria","Bamfordvirae","Nucleocytoviricota","Pokkesviricetes","Chitovirales","Poxviridae","Chordopoxvirinae","Orthopoxvirus","Vaccinia virus"]},"organismHosts":[{"scientificName":"Bos taurus","commonName":"Bovine","taxonId":9913}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Virion membrane protein OPG141"}}},"genes":[{"geneName":{"value":"OPG141"},"orderedLocusNames":[{"value":"VACWR134"}],"orfNames":[{"value":"A 14.5L"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10756020"}],"value":"Protein probably involved in counteracting host defense, since it enhances virulence in vivo"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10756020"}],"value":"Component of the mature virion (MV) membrane"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Virion membrane","id":"SL-0275"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10756020"}],"value":"Expressed in the late phase of the viral replicative cycle"}],"commentType":"INDUCTION"},{"texts":[{"value":"Not phosphorylated"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the orthopoxvirus OPG141 protein family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"Virion membrane protein OPG141","featureId":"PRO_0000414116"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"Intravirion","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":5,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"Virion surface","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":50,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"Intravirion","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0347","category":"Molecular function","name":"Helicase"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0426","category":"Developmental stage","name":"Late protein"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"},{"id":"KW-0806","category":"Biological process","name":"Transcription termination"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0261","category":"Cellular component","name":"Viral envelope protein"},{"id":"KW-0946","category":"Cellular component","name":"Virion"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-62F9VR5MBT7LF","citationType":"submission","authors":["Esposito J.J.","Frace A.M.","Sammons S.A.","Olsen-Rasmussen M.","Osborne J.","Wohlhueter R."],"title":"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb.","publicationDate":"FEB-2003","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"]},{"referenceNumber":2,"citation":{"id":"10756020","citationType":"journal article","authors":["Betakova T.","Wolffe E.J.","Moss B."],"citationCrossReferences":[{"database":"PubMed","id":"10756020"},{"database":"DOI","id":"10.1128/jvi.74.9.4085-4092.2000"}],"title":"The vaccinia virus A14.5L gene encodes a hydrophobic 53-amino-acid virion membrane protein that enhances virulence in mice and is conserved among vertebrate poxviruses.","publicationDate":"2000","journal":"J. Virol.","firstPage":"4085","lastPage":"4092","volume":"74"},"referencePositions":["FUNCTION","INDUCTION","SUBCELLULAR LOCATION","ABSENCE OF PHOSPHORYLATION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY243312","properties":[{"key":"ProteinId","value":"AAO89413.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"YP_233016.1","properties":[{"key":"NucleotideSequenceId","value":"NC_006998.1"}]},{"database":"AlphaFoldDB","id":"Q80HV6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q80HV6","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"3508886","properties":[{"key":"Interactions","value":"9"}]},{"database":"DNASU","id":"3707532","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"3707532","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"vg:3707532","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q80HV6","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000344","properties":[{"key":"Component","value":"Segment"}]},{"database":"GO","id":"GO:0019031","properties":[{"key":"GoTerm","value":"C:viral envelope"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0055036","properties":[{"key":"GoTerm","value":"C:virion membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0003677","properties":[{"key":"GoTerm","value":"F:DNA binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0004386","properties":[{"key":"GoTerm","value":"F:helicase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0016787","properties":[{"key":"GoTerm","value":"F:hydrolase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0006353","properties":[{"key":"GoTerm","value":"P:DNA-templated transcription termination"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"InterPro","id":"IPR009372","properties":[{"key":"EntryName","value":"Poxvirus_A14.5"}]},{"database":"Pfam","id":"PF06269","properties":[{"key":"EntryName","value":"DUF1029"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MISNYEPLLLLVITCCVLLFNFTISSKTKIDIIFAVQTIVFIWFIFHFVHSAI","length":53,"molWeight":6165,"crc64":"892885E0E0348A8E","md5":"CA2C8B69ACAFFA6EC7362389E4B42130"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Topological domain":3,"Transmembrane":2},"uniParcId":"UPI00000ED0BA"}}