{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q7TSI1","secondaryAccessions":["Q3U3L2","Q8CHU5"],"uniProtkbId":"PKHM1_MOUSE","entryAudit":{"firstPublicDate":"2007-11-13","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-10-01","entryVersion":151,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Pleckstrin homology domain-containing family M member 1"},"shortNames":[{"value":"PH domain-containing family M member 1"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:2443207"}],"value":"Plekhm1"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25498145"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27777970"}],"value":"Acts as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Acts as a dual effector of RAB7A and ARL8B that simultaneously binds these GTPases, bringing about clustering and fusion of late endosomes and lysosomes. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Interaction with Arl8b is a crucial factor in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (PubMed:25498145). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:27777970). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7. May have a role in sialyl-lex-mediated transduction of apoptotic signals (By similarity). Involved in bone resorption (PubMed:27777970)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22073305"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27777970"}],"value":"Interacts (via N- and C-terminus) with RAB7A (GTP-bound form). Simultaneously interacts with RAB7A and ARL8B; bringing about clustering and fusion of late endosomes and lysosomes. Interacts (via RUN domain) with ARL8B (GTP-bound form); the interaction is required for PLEKHM1 localization to lysosomes and for ARL8B function in delivery and degradation of endocytic and autophagic cargo in lysosomes. PLEKHM1 and PLEKHM2 compete for interaction with ARL8B. Interacts with ARL8A; the interaction is weaker than with ARL8B. Interacts with VPS41, VPS11, VPS18, VPS33A and VPS39; indicative for an association with the HOPS complex; the interactions with, at least, VPS41, VPS11, VPS18 and VPS33A require ARL8B (By similarity). Interacts with GABARAP, GABARAPL, GABARAPL2, MAP1LC3A, MAP1LC3B and MAP1LC3C (By similarity). Interacts with PAFAH1B (PubMed:22073305). Interacts (via N- and C-terminus) with NDEL1 (PubMed:27777970). Interacts (via C-terminus) with MAP3K7 (PubMed:27777970). Interacts (via N- and C-terminus) with FAM98A (PubMed:27777970). Interacts (via C-terminus) with DEF8; this interaction is weak but increased in a RAB7A-dependent manner (PubMed:27777970). May interact with sialyl-lex-positive protein (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}],"value":"In case of infection colocalizes with Salmonella typhimurium sifA in proximity of Salmonella-containing vacuole (SCV)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}],"value":"Autolysosome membrane","id":"SL-0536"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}],"value":"Endosome membrane","id":"SL-0100"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}],"value":"Late endosome membrane","id":"SL-0151"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}],"value":"Lysosome membrane","id":"SL-0157"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}],"value":"The LIR (LC3-interacting region) motif mediates the interaction with ATG8 family proteins GABARAP, GABARAPL, GABARAPL2, and LC3A/B/C"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27777970"}],"value":"Osteoclast-specific conditional knockout mice show normal tooth eruption, developed normally and are fertile, but trabecular bone mass in long bones and vertebrae is increased (PubMed:27777970). Osteoclast differentiation and number are normal, but bone resorption is decreased (PubMed:27777970). Show mislocalization of osteoclast lysosomes at the perinuclear area, instead at the cell periphery, and decreased ruffled border formation (PubMed:27777970)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"value":"Sialyl-lex is a carcinoma associated antigen"}],"commentType":"MISCELLANEOUS"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1074,"modifier":"EXACT"}},"description":"Pleckstrin homology domain-containing family M member 1","featureId":"PRO_0000309336"},{"type":"Domain","location":{"start":{"value":40,"modifier":"EXACT"},"end":{"value":182,"modifier":"EXACT"}},"description":"RUN","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00178"}]},{"type":"Domain","location":{"start":{"value":551,"modifier":"EXACT"},"end":{"value":642,"modifier":"EXACT"}},"description":"PH 1","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00145"}]},{"type":"Domain","location":{"start":{"value":701,"modifier":"EXACT"},"end":{"value":795,"modifier":"EXACT"}},"description":"PH 2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00145"}]},{"type":"Zinc finger","location":{"start":{"value":1004,"modifier":"EXACT"},"end":{"value":1058,"modifier":"EXACT"}},"description":"Phorbol-ester/DAG-type","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00226"}]},{"type":"Region","location":{"start":{"value":214,"modifier":"EXACT"},"end":{"value":244,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":272,"modifier":"EXACT"},"end":{"value":336,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":382,"modifier":"EXACT"},"end":{"value":454,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":506,"modifier":"EXACT"},"end":{"value":526,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":661,"modifier":"EXACT"},"end":{"value":680,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":672,"modifier":"EXACT"},"end":{"value":1074,"modifier":"EXACT"}},"description":"Interaction with RAB7A","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}]},{"type":"Motif","location":{"start":{"value":649,"modifier":"EXACT"},"end":{"value":655,"modifier":"EXACT"}},"description":"LIR","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}]},{"type":"Compositional bias","location":{"start":{"value":313,"modifier":"EXACT"},"end":{"value":329,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":393,"modifier":"EXACT"},"end":{"value":404,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":511,"modifier":"EXACT"},"end":{"value":525,"modifier":"EXACT"}},"description":"Pro residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":218,"modifier":"EXACT"},"end":{"value":218,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":433,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y4G2"}]},{"type":"Modified residue","location":{"start":{"value":436,"modifier":"EXACT"},"end":{"value":436,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":491,"modifier":"EXACT"},"end":{"value":491,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Mutagenesis","location":{"start":{"value":714,"modifier":"EXACT"},"end":{"value":714,"modifier":"EXACT"}},"description":"No change in osteoclast formation and bone resorption.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27777970"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["C"]}},{"type":"Mutagenesis","location":{"start":{"value":949,"modifier":"EXACT"},"end":{"value":950,"modifier":"EXACT"}},"description":"Decreases interaction with RAB7A.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27777970"}],"alternativeSequence":{"originalSequence":"YL","alternativeSequences":["AA"]}},{"type":"Sequence conflict","location":{"start":{"value":742,"modifier":"EXACT"},"end":{"value":744,"modifier":"EXACT"}},"description":"in Ref. 3; AAH38943","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"DSH","alternativeSequences":["TRP"]}},{"type":"Sequence conflict","location":{"start":{"value":768,"modifier":"EXACT"},"end":{"value":768,"modifier":"EXACT"}},"description":"in Ref. 1; BAE32773","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0072","category":"Biological process","name":"Autophagy"},{"id":"KW-0968","category":"Cellular component","name":"Cytoplasmic vesicle"},{"id":"KW-0967","category":"Cellular component","name":"Endosome"},{"id":"KW-0458","category":"Cellular component","name":"Lysosome"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0653","category":"Biological process","name":"Protein transport"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0813","category":"Biological process","name":"Transport"},{"id":"KW-0862","category":"Ligand","name":"Zinc"},{"id":"KW-0863","category":"Domain","name":"Zinc-finger"}],"references":[{"referenceNumber":1,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"NOD","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"19468303","citationType":"journal article","authors":["Church D.M.","Goodstadt L.","Hillier L.W.","Zody M.C.","Goldstein S.","She X.","Bult C.J.","Agarwala R.","Cherry J.L.","DiCuccio M.","Hlavina W.","Kapustin Y.","Meric P.","Maglott D.","Birtle Z.","Marques A.C.","Graves T.","Zhou S.","Teague B.","Potamousis K.","Churas C.","Place M.","Herschleb J.","Runnheim R.","Forrest D.","Amos-Landgraf J.","Schwartz D.C.","Cheng Z.","Lindblad-Toh K.","Eichler E.E.","Ponting C.P."],"citationCrossReferences":[{"database":"PubMed","id":"19468303"},{"database":"DOI","id":"10.1371/journal.pbio.1000112"}],"title":"Lineage-specific biology revealed by a finished genome assembly of the mouse.","publicationDate":"2009","journal":"PLoS Biol.","firstPage":"E1000112","lastPage":"E1000112","volume":"7"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"FVB/N","type":"STRAIN"},{"value":"Brain","type":"TISSUE"},{"value":"Kidney","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-218; SER-436 AND SER-491","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Brown adipose tissue","type":"TISSUE"},{"value":"Spleen","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"22073305","citationType":"journal article","authors":["Ye S.","Fowler T.W.","Pavlos N.J.","Ng P.Y.","Liang K.","Feng Y.","Zheng M.","Kurten R.","Manolagas S.C.","Zhao H."],"citationCrossReferences":[{"database":"PubMed","id":"22073305"},{"database":"DOI","id":"10.1371/journal.pone.0027285"}],"title":"LIS1 regulates osteoclast formation and function through its interactions with dynein/dynactin and Plekhm1.","publicationDate":"2011","journal":"PLoS ONE","firstPage":"E27285","lastPage":"E27285","volume":"6"},"referencePositions":["INTERACTION WITH PAFAH1B1"]},{"referenceNumber":6,"citation":{"id":"25498145","citationType":"journal article","authors":["McEwan D.G.","Popovic D.","Gubas A.","Terawaki S.","Suzuki H.","Stadel D.","Coxon F.P.","Miranda de Stegmann D.","Bhogaraju S.","Maddi K.","Kirchof A.","Gatti E.","Helfrich M.H.","Wakatsuki S.","Behrends C.","Pierre P.","Dikic I."],"citationCrossReferences":[{"database":"PubMed","id":"25498145"},{"database":"DOI","id":"10.1016/j.molcel.2014.11.006"}],"title":"PLEKHM1 regulates autophagosome-lysosome fusion through HOPS complex and LC3/GABARAP proteins.","publicationDate":"2015","journal":"Mol. Cell","firstPage":"39","lastPage":"54","volume":"57"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION"]},{"referenceNumber":7,"citation":{"id":"27777970","citationType":"journal article","authors":["Fujiwara T.","Ye S.","Castro-Gomes T.","Winchell C.G.","Andrews N.W.","Voth D.E.","Varughese K.I.","Mackintosh S.G.","Feng Y.","Pavlos N.","Nakamura T.","Manolagas S.C.","Zhao H."],"citationCrossReferences":[{"database":"PubMed","id":"27777970"},{"database":"DOI","id":"10.1172/jci.insight.86330"}],"title":"PLEKHM1/DEF8/RAB7 complex regulates lysosome positioning and bone homeostasis.","publicationDate":"2016","journal":"JCI Insight","firstPage":"E86330","lastPage":"E86330","volume":"1"},"referencePositions":["FUNCTION","INTERACTION WITH DEF8; FAM98A; NDEL1 AND RAB7A","DISRUPTION PHENOTYPE","MUTAGENESIS OF ARG-714 AND 949-TYR-LEU-950"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK154703","properties":[{"key":"ProteinId","value":"BAE32773.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AL772325","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AL929552","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BC038943","properties":[{"key":"ProteinId","value":"AAH38943.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC053079","properties":[{"key":"ProteinId","value":"AAH53079.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS25518.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_898855.1","properties":[{"key":"NucleotideSequenceId","value":"NM_183034.2"}]},{"database":"AlphaFoldDB","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"237251","properties":[{"key":"Interactions","value":"1"}]},{"database":"FunCoup","id":"Q7TSI1","properties":[{"key":"Number of interactors","value":"3214"}]},{"database":"IntAct","id":"Q7TSI1","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"10090.ENSMUSP00000047327","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000047327","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"289911","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"30012","properties":[{"key":"antibodies","value":"144 antibodies from 28 providers"}]},{"database":"Ensembl","id":"ENSMUST00000041272.10","properties":[{"key":"ProteinId","value":"ENSMUSP00000047327.9"},{"key":"GeneId","value":"ENSMUSG00000034247.10"}]},{"database":"GeneID","id":"353047","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:353047","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc007lug.1","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:2443207","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"9842","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:2443207","properties":[{"key":"GeneName","value":"Plekhm1"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000034247","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG1829","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000155111","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_011318_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"IWRDYYC","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"62364at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"353047","properties":[{"key":"hits","value":"3 hits in 76 CRISPR screens"}]},{"database":"PRO","id":"PR:Q7TSI1","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 11"}]},{"database":"RNAct","id":"Q7TSI1","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000034247","properties":[{"key":"ExpressionPatterns","value":"Expressed in granulocyte and 191 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q7TSI1","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0044754","properties":[{"key":"GoTerm","value":"C:autolysosome"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0120281","properties":[{"key":"GoTerm","value":"C:autolysosome membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0010008","properties":[{"key":"GoTerm","value":"C:endosome membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0031902","properties":[{"key":"GoTerm","value":"C:late endosome membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005765","properties":[{"key":"GoTerm","value":"C:lysosomal membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005764","properties":[{"key":"GoTerm","value":"C:lysosome"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005730","properties":[{"key":"GoTerm","value":"C:nucleolus"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0061909","properties":[{"key":"GoTerm","value":"P:autophagosome-lysosome fusion"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:1902774","properties":[{"key":"GoTerm","value":"P:late endosome to lysosome transport"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0032418","properties":[{"key":"GoTerm","value":"P:lysosome localization"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27777970"}]},{"database":"GO","id":"GO:0045780","properties":[{"key":"GoTerm","value":"P:positive regulation of bone resorption"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27777970"}]},{"database":"GO","id":"GO:1900029","properties":[{"key":"GoTerm","value":"P:positive regulation of ruffle assembly"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"27777970"}]},{"database":"CDD","id":"cd13321","properties":[{"key":"EntryName","value":"PH_PLEKHM1"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd17679","properties":[{"key":"EntryName","value":"RUN_PLEKHM1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.58.900:FF:000013","properties":[{"key":"EntryName","value":"pleckstrin homology domain-containing family M member 1 isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.30.29.30:FF:000315","properties":[{"key":"EntryName","value":"pleckstrin homology domain-containing family M member 1 isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.58.900","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.29.30","properties":[{"key":"EntryName","value":"Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR051366","properties":[{"key":"EntryName","value":"DEF8"}]},{"database":"InterPro","id":"IPR011993","properties":[{"key":"EntryName","value":"PH-like_dom_sf"}]},{"database":"InterPro","id":"IPR001849","properties":[{"key":"EntryName","value":"PH_domain"}]},{"database":"InterPro","id":"IPR002219","properties":[{"key":"EntryName","value":"PKC_DAG/PE"}]},{"database":"InterPro","id":"IPR042827","properties":[{"key":"EntryName","value":"PLEKHM1_PH"}]},{"database":"InterPro","id":"IPR025258","properties":[{"key":"EntryName","value":"RH_dom"}]},{"database":"InterPro","id":"IPR004012","properties":[{"key":"EntryName","value":"Run_dom"}]},{"database":"InterPro","id":"IPR037213","properties":[{"key":"EntryName","value":"Run_dom_sf"}]},{"database":"InterPro","id":"IPR047326","properties":[{"key":"EntryName","value":"RUN_PLEKHM1"}]},{"database":"PANTHER","id":"PTHR12326","properties":[{"key":"EntryName","value":"PLECKSTRIN HOMOLOGY DOMAIN CONTAINING PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12326:SF5","properties":[{"key":"EntryName","value":"PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY M MEMBER 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13901","properties":[{"key":"EntryName","value":"RH_dom"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02759","properties":[{"key":"EntryName","value":"RUN"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00109","properties":[{"key":"EntryName","value":"C1"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM01175","properties":[{"key":"EntryName","value":"DUF4206"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00233","properties":[{"key":"EntryName","value":"PH"},{"key":"MatchStatus","value":"2"}]},{"database":"SMART","id":"SM00593","properties":[{"key":"EntryName","value":"RUN"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50729","properties":[{"key":"EntryName","value":"PH domain-like"},{"key":"MatchStatus","value":"2"}]},{"database":"SUPFAM","id":"SSF140741","properties":[{"key":"EntryName","value":"RUN domain-like"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50003","properties":[{"key":"EntryName","value":"PH_DOMAIN"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS50826","properties":[{"key":"EntryName","value":"RUN"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50081","properties":[{"key":"EntryName","value":"ZF_DAG_PE_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MLSVENGLDPRAAIQVIKKKLVGSVKALQKQHVSLDTVVTSEDGDANTMCSALEAVFIHGLHAKHIRAEAGGKRKKHTHQKALPQPVFWPLLKAITHRHIVSDLEHLVFINTDVGRCRAWLRLALNDGLMECYLKLLLQEPARLCEYYQPTALLRDAEEAEFLLSFLQGLTSLSFELSYKSAILNEWTLTPLSLSGLCPLSELDPLTTSGAELQRKESLDSISHSSGSEDIEVQHSGHKIRRNRKLTASSLSLDTASSSQLSCSLNSDSCLLQENGPKSPDHSEEPMSYDSDLGMANTDDPDRSLQEVLSEFSKAQVNSAPSSGPNQEPDTPMFQTPLSLHSLATSTHLHFEGSEELFPAHKSSGTSSGGHKHQLLPQETPDEKQLGTAQAGPAQSTSDQQPSSPVGGAAGQGSGPWKALEYGRVGPKLVVSSPTSPKGKSWISEDDFCRPPQEPALKSAAGLCTSPVQDTPESRAALHGPFSQGPRKSCSLGALDKACVPSQACGNAQPAPAPAPAPAPAPAPAPGVTQDHKNFCVVHRRQMGLSNPFRGLMKLGTVARRGAMGIWKEFFCELSPLEFRLYLSDEERTCVESCSLLRCEAVGPAHSDGRFELVFSGKKLALRASSQDEAEDWLDRVREALQKVRPQQEDEWVNIQYPDQAEDAPEAPPDSLPPYSTLLPEPAGAQGMQLDWTSAQVPEPDAIKESLLYLYADRTWVPYIFSLSLESLKCFRVRNNEKMLSDSHGVETIRDILPDTSLGGPAFFKIITAKAVLKLQAKNTEEATHWRDLVRKVLASYLESAEEAVTLGGSLDEKCQEVLKFATRENGFLLQYLVAIPTEKGLDSQGCFCAGCSRQIGFSFVRPKLCAFSGLYYCDFCHQDDASVIPARIIHNWDLTKRPVCRQALKFLAQIRAQPLINLQLVNASLYEHVERMHLIGRSREQLKLLGDYLGLCRSGALKELCKRLSHRNYLLESPHRFSVADLQQIAEGVYEGFLKALIEFASQHVYHCDLCTQRGFICQICHHQDIIFPFEFDTTVRCAECRTVFHQSCQAVVRKGCPRCARRRKYQEQNVVS","length":1074,"molWeight":118535,"crc64":"56EDA05514E4B9F9","md5":"0F24D3BC81D798960C5BBD6D6B973262"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DOMAIN":1,"DISRUPTION PHENOTYPE":1,"MISCELLANEOUS":1},"countByFeatureType":{"Chain":1,"Domain":3,"Zinc finger":1,"Region":6,"Motif":1,"Compositional bias":3,"Modified residue":4,"Mutagenesis":2,"Sequence conflict":2},"uniParcId":"UPI00001A3D23"}}