{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q6R3M4","secondaryAccessions":["Q641P1","Q6R3M3","Q9R1A6"],"uniProtkbId":"POLI_MOUSE","entryAudit":{"firstPublicDate":"2005-02-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":157,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"DNA polymerase iota"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"value":"2.7.7.7"}]},"alternativeNames":[{"fullName":{"value":"Rad30 homolog B"}}]},"genes":[{"geneName":{"value":"Poli"},"synonyms":[{"value":"Rad30b"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"value":"Error-prone DNA polymerase specifically involved in DNA repair (PubMed:15026325, PubMed:16357261). Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:15026325, PubMed:16357261). Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template (By similarity). Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine (By similarity). May play a role in hypermutation of immunoglobulin genes (By similarity). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:22508"},{"database":"Rhea","id":"RHEA-COMP:17339"},{"database":"Rhea","id":"RHEA-COMP:17340"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:61560"},{"database":"ChEBI","id":"CHEBI:173112"}],"ecNumber":"2.7.7.7","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}},{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"value":"Binds nucleotide much more tightly and catalyzes nucleotide insertion much more efficiently in the presence of Mg(2+) than in the presence of Mn(2+)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14657033"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"value":"Interacts with POLH (By similarity). Interacts with REV1 (PubMed:14657033). Interacts with ubiquitin (PubMed:16357261)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"value":"Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Nucleus","id":"SL-0191"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q6R3M4-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q6R3M4-2"],"sequenceIds":["VSP_012800"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10458907"}],"value":"Detected in testis, and at very low levels in spleen, lung and brain. Detected in round spermatids, but not in prophase spermatocytes"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"value":"The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"value":"Ubiquitin-binding motif 1 and ubiquitin-binding motif 2 regulate POLI protein monoubiquitination and localization to nuclear foci after UV-induced DNA damage"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"value":"Monoubiquitinated (PubMed:16357261). Protein monoubiquitination prevents POLI binding to ubiquitin via the ubiquitin-binding motif 1 and ubiquitin-binding motif 2 (PubMed:16357261)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the DNA polymerase type-Y family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":717,"modifier":"EXACT"}},"description":"DNA polymerase iota","featureId":"PRO_0000173989"},{"type":"Domain","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":243,"modifier":"EXACT"}},"description":"UmuC","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00216"}]},{"type":"Domain","location":{"start":{"value":489,"modifier":"EXACT"},"end":{"value":527,"modifier":"EXACT"}},"description":"UBM 1","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01409"}]},{"type":"Domain","location":{"start":{"value":677,"modifier":"EXACT"},"end":{"value":712,"modifier":"EXACT"}},"description":"UBM 2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01409"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":22,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":300,"modifier":"EXACT"},"end":{"value":307,"modifier":"EXACT"}},"description":"DNA-binding","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}]},{"type":"Region","location":{"start":{"value":343,"modifier":"EXACT"},"end":{"value":360,"modifier":"EXACT"}},"description":"DNA-binding","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}]},{"type":"Region","location":{"start":{"value":549,"modifier":"EXACT"},"end":{"value":589,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":603,"modifier":"EXACT"},"end":{"value":622,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":644,"modifier":"EXACT"},"end":{"value":687,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Motif","location":{"start":{"value":500,"modifier":"EXACT"},"end":{"value":517,"modifier":"EXACT"}},"description":"Ubiquitin-binding 1 (UBM1)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}]},{"type":"Motif","location":{"start":{"value":685,"modifier":"EXACT"},"end":{"value":702,"modifier":"EXACT"}},"description":"Ubiquitin-binding 2 (UBM2)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}]},{"type":"Compositional bias","location":{"start":{"value":575,"modifier":"EXACT"},"end":{"value":589,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":607,"modifier":"EXACT"},"end":{"value":622,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":652,"modifier":"EXACT"},"end":{"value":662,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":665,"modifier":"EXACT"},"end":{"value":679,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00216"}]},{"type":"Binding site","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":34,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61560"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"ligand":{"name":"a 2'-deoxyribonucleoside 5'-triphosphate","id":"ChEBI:CHEBI:61560"}},{"type":"Binding site","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":71,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61560"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"ligand":{"name":"a 2'-deoxyribonucleoside 5'-triphosphate","id":"ChEBI:CHEBI:61560"}},{"type":"Binding site","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9UNA4"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":43,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15489334"}],"featureId":"VSP_012800","alternativeSequence":{}},{"type":"Natural variant","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":4,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10458907"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"L","alternativeSequences":["S"]}},{"type":"Natural variant","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":7,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10458907"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}},{"type":"Natural variant","location":{"start":{"value":334,"modifier":"EXACT"},"end":{"value":334,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"Q","alternativeSequences":["K"]}},{"type":"Natural variant","location":{"start":{"value":378,"modifier":"EXACT"},"end":{"value":378,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Natural variant","location":{"start":{"value":518,"modifier":"EXACT"},"end":{"value":518,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"10458907"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15489334"}],"featureId":"","alternativeSequence":{"originalSequence":"Y","alternativeSequences":["S"]}},{"type":"Natural variant","location":{"start":{"value":524,"modifier":"EXACT"},"end":{"value":524,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"N","alternativeSequences":["Y"]}},{"type":"Natural variant","location":{"start":{"value":569,"modifier":"EXACT"},"end":{"value":569,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"A","alternativeSequences":["E"]}},{"type":"Natural variant","location":{"start":{"value":574,"modifier":"EXACT"},"end":{"value":574,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"E","alternativeSequences":["D"]}},{"type":"Natural variant","location":{"start":{"value":606,"modifier":"EXACT"},"end":{"value":606,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"Q","alternativeSequences":["R"]}},{"type":"Natural variant","location":{"start":{"value":643,"modifier":"EXACT"},"end":{"value":643,"modifier":"EXACT"}},"description":"in strain: BALB/c","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}],"featureId":"","alternativeSequence":{"originalSequence":"T","alternativeSequences":["I"]}},{"type":"Mutagenesis","location":{"start":{"value":496,"modifier":"EXACT"},"end":{"value":524,"modifier":"EXACT"}},"description":"Abolishes ubiquitin binding and localization to nuclear foci after UV-induced DNA damage.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":508,"modifier":"EXACT"},"end":{"value":508,"modifier":"EXACT"}},"description":"Impairs ubiquitin binding and post-translation modification via ubiquitination; when associated with A-509. Abolishes ubiquitin binding and localization to nuclear foci after UV-induced DNA damage but does not affect catalytic activity; when associated with A-508, A-693 and A-694.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":509,"modifier":"EXACT"},"end":{"value":509,"modifier":"EXACT"}},"description":"Impairs ubiquitin binding and post-translation modification via ubiquitination; when associated with A-508. Abolishes ubiquitin binding and localization to nuclear foci after UV-induced DNA damage but does not affect catalytic activity; when associated with A-509, A-693 and A-694.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"alternativeSequence":{"originalSequence":"P","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":681,"modifier":"EXACT"},"end":{"value":709,"modifier":"EXACT"}},"description":"Abolishes ubiquitin binding and localization to nuclear foci after UV-induced DNA damage.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":693,"modifier":"EXACT"},"end":{"value":693,"modifier":"EXACT"}},"description":"Impairs ubiquitin binding and post-translation modification via ubiquitination; when associated with A-694. Abolishes ubiquitin binding and localization to nuclear foci after UV-induced DNA damage but does not affect catalytic activity; when associated with A-508, A-509 and A-694.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":694,"modifier":"EXACT"},"end":{"value":694,"modifier":"EXACT"}},"description":"Impairs ubiquitin binding and post-translation modification via ubiquitination; when associated with A-693. Abolishes ubiquitin binding and localization to nuclear foci after UV-induced DNA damage but does not affect catalytic activity; when associated with A-508, A-509 and A-693.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16357261"}],"alternativeSequence":{"originalSequence":"P","alternativeSequences":["A"]}},{"type":"Sequence conflict","location":{"start":{"value":583,"modifier":"EXACT"},"end":{"value":583,"modifier":"EXACT"}},"description":"in Ref. 1; AAD50424","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}},{"type":"Helix","location":{"start":{"value":502,"modifier":"EXACT"},"end":{"value":504,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2KWV"}]},{"type":"Turn","location":{"start":{"value":505,"modifier":"EXACT"},"end":{"value":507,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2KWV"}]},{"type":"Helix","location":{"start":{"value":510,"modifier":"EXACT"},"end":{"value":516,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2KWV"}]},{"type":"Beta strand","location":{"start":{"value":682,"modifier":"EXACT"},"end":{"value":684,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"2KWU"}]},{"type":"Helix","location":{"start":{"value":687,"modifier":"EXACT"},"end":{"value":690,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3AI4"}]},{"type":"Helix","location":{"start":{"value":695,"modifier":"EXACT"},"end":{"value":700,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3AI4"}]},{"type":"Helix","location":{"start":{"value":703,"modifier":"EXACT"},"end":{"value":705,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3AI4"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"id":"KW-0235","category":"Biological process","name":"DNA replication"},{"id":"KW-0237","category":"Biological process","name":"DNA synthesis"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0239","category":"Molecular function","name":"DNA-directed DNA polymerase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0515","category":"Molecular function","name":"Mutator protein"},{"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0704","category":"Ligand","name":"Schiff base"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0832","category":"PTM","name":"Ubl conjugation"}],"references":[{"referenceNumber":1,"citation":{"id":"10458907","citationType":"journal article","authors":["McDonald J.P.","Rapic-Otrin V.","Epstein J.A.","Broughton B.C.","Wang X.","Lehmann A.R.","Wolgemuth D.J.","Woodgate R."],"citationCrossReferences":[{"database":"PubMed","id":"10458907"},{"database":"DOI","id":"10.1006/geno.1999.5906"}],"title":"Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta.","publicationDate":"1999","journal":"Genomics","firstPage":"20","lastPage":"30","volume":"60"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)","VARIANTS SER-4; ARG-7 AND SER-518","TISSUE SPECIFICITY"],"referenceComments":[{"value":"BALB/cJ","type":"STRAIN"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15026325","citationType":"journal article","authors":["Wang M.","Devereux T.R.","Vikis H.G.","McCulloch S.D.","Holliday W.","Anna C.","Wang Y.","Bebenek K.","Kunkel T.A.","Guan K.","You M."],"citationCrossReferences":[{"database":"PubMed","id":"15026325"},{"database":"DOI","id":"10.1158/0008-5472.can-03-3080"}],"title":"Pol iota is a candidate for the mouse pulmonary adenoma resistance 2 locus, a major modifier of chemically induced lung neoplasia.","publicationDate":"2004","journal":"Cancer Res.","firstPage":"1924","lastPage":"1931","volume":"64"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)","FUNCTION","CATALYTIC ACTIVITY","VARIANTS SER-4; ARG-7; LYS-334; ALA-378; SER-518; TYR-524; GLU-569; ASP-574; ARG-606 AND ILE-643","ALTERNATIVE SPLICING"],"referenceComments":[{"value":"A/J","type":"STRAIN"},{"value":"BALB/cJ","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)","VARIANT SER-518"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Embryo","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"14657033","citationType":"journal article","authors":["Guo C.","Fischhaber P.L.","Luk-Paszyc M.J.","Masuda Y.","Zhou J.","Kamiya K.","Kisker C.","Friedberg E.C."],"citationCrossReferences":[{"database":"PubMed","id":"14657033"},{"database":"DOI","id":"10.1093/emboj/cdg626"}],"title":"Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis.","publicationDate":"2003","journal":"EMBO J.","firstPage":"6621","lastPage":"6630","volume":"22"},"referencePositions":["INTERACTION WITH REV1"]},{"referenceNumber":5,"citation":{"id":"16357261","citationType":"journal article","authors":["Bienko M.","Green C.M.","Crosetto N.","Rudolf F.","Zapart G.","Coull B.","Kannouche P.","Wider G.","Peter M.","Lehmann A.R.","Hofmann K.","Dikic I."],"citationCrossReferences":[{"database":"PubMed","id":"16357261"},{"database":"DOI","id":"10.1126/science.1120615"}],"title":"Ubiquitin-binding domains in Y-family polymerases regulate translesion synthesis.","publicationDate":"2005","journal":"Science","firstPage":"1821","lastPage":"1824","volume":"310"},"referencePositions":["FUNCTION","INTERACTION WITH UBIQUITIN","SUBCELLULAR LOCATION","UBIQUITINATION","MOTIF","MUTAGENESIS OF LEU-508; PRO-509; LEU-693; PRO-694; 496-LEU--ASN-524 AND 681-PHE--GLY-709"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF151691","properties":[{"key":"ProteinId","value":"AAD50424.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY515316","properties":[{"key":"ProteinId","value":"AAS75834.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY515317","properties":[{"key":"ProteinId","value":"AAS75835.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC082278","properties":[{"key":"ProteinId","value":"AAH82278.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS50317.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q6R3M4-2"},{"database":"RefSeq","id":"NP_001129562.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001136090.3"}]},{"database":"RefSeq","id":"NP_001276445.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001289516.2"}]},{"database":"PDB","id":"2KWU","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=673-717"}]},{"database":"PDB","id":"2KWV","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=487-532"}]},{"database":"PDB","id":"3AI4","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.60 A"},{"key":"Chains","value":"A=668-717"}]},{"database":"PDBsum","id":"2KWU","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"2KWV","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3AI4","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"BMRB","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"204998","properties":[{"key":"Interactions","value":"6"}]},{"database":"FunCoup","id":"Q6R3M4","properties":[{"key":"Number of interactors","value":"1862"}]},{"database":"IntAct","id":"Q6R3M4","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"10090.ENSMUSP00000112563","properties":[{"key":"Description","value":"-"}]},{"database":"BindingDB","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"ChEMBL","id":"CHEMBL5159","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q6R3M4","properties":[{"key":"glycosylation","value":"2 sites"}]},{"database":"iPTMnet","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000112563","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"289945","properties":[{"key":"Description","value":"-"}],"isoformId":"Q6R3M4-1"},{"database":"ProteomicsDB","id":"289946","properties":[{"key":"Description","value":"-"}],"isoformId":"Q6R3M4-2"},{"database":"GeneID","id":"26447","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:26447","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"MGI:1347081","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"11201","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1347081","properties":[{"key":"GeneName","value":"Poli"}]},{"database":"eggNOG","id":"KOG2095","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"447129at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-MMU-5656121","properties":[{"key":"PathwayName","value":"Translesion synthesis by POLI"}]},{"database":"Reactome","id":"R-MMU-5656169","properties":[{"key":"PathwayName","value":"Termination of translesion DNA synthesis"}]},{"database":"BioGRID-ORCS","id":"26447","properties":[{"key":"hits","value":"1 hit in 113 CRISPR screens"}]},{"database":"EvolutionaryTrace","id":"Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q6R3M4","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Unplaced"}]},{"database":"RNAct","id":"Q6R3M4","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0036464","properties":[{"key":"GoTerm","value":"C:cytoplasmic ribonucleoprotein granule"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0016607","properties":[{"key":"GoTerm","value":"C:nuclear speck"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0003684","properties":[{"key":"GoTerm","value":"F:damaged DNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0034061","properties":[{"key":"GoTerm","value":"F:DNA polymerase activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15026325"}]},{"database":"GO","id":"GO:0003887","properties":[{"key":"GoTerm","value":"F:DNA-directed DNA polymerase activity"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"12925679"}]},{"database":"GO","id":"GO:0071494","properties":[{"key":"GoTerm","value":"P:cellular response to UV-C"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"17114294"}]},{"database":"GO","id":"GO:0006281","properties":[{"key":"GoTerm","value":"P:DNA repair"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0019985","properties":[{"key":"GoTerm","value":"P:translesion synthesis"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"17114294"}]},{"database":"CDD","id":"cd01703","properties":[{"key":"EntryName","value":"PolY_Pol_iota"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.1170.60:FF:000007","properties":[{"key":"EntryName","value":"DNA polymerase iota"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.1490.100:FF:000003","properties":[{"key":"EntryName","value":"Polymerase (DNA directed) iota"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.30.70.270:FF:000013","properties":[{"key":"EntryName","value":"Polymerase (DNA directed) iota"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.270","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1170.60","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"6.10.250.1630","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"2"}]},{"database":"Gene3D","id":"1.10.150.20","properties":[{"key":"EntryName","value":"5' to 3' exonuclease, C-terminal subdomain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1490.100","properties":[{"key":"EntryName","value":"DNA polymerase, Y-family, little finger domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR043502","properties":[{"key":"EntryName","value":"DNA/RNA_pol_sf"}]},{"database":"InterPro","id":"IPR036775","properties":[{"key":"EntryName","value":"DNA_pol_Y-fam_lit_finger_sf"}]},{"database":"InterPro","id":"IPR017961","properties":[{"key":"EntryName","value":"DNA_pol_Y-fam_little_finger"}]},{"database":"InterPro","id":"IPR025527","properties":[{"key":"EntryName","value":"HUWE1/Rev1_UBM"}]},{"database":"InterPro","id":"IPR053848","properties":[{"key":"EntryName","value":"IMS_HHH_1"}]},{"database":"InterPro","id":"IPR043128","properties":[{"key":"EntryName","value":"Rev_trsase/Diguanyl_cyclase"}]},{"database":"InterPro","id":"IPR001126","properties":[{"key":"EntryName","value":"UmuC"}]},{"database":"PANTHER","id":"PTHR46404","properties":[{"key":"EntryName","value":"DNA POLYMERASE IOTA"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR46404:SF1","properties":[{"key":"EntryName","value":"DNA POLYMERASE IOTA"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00817","properties":[{"key":"EntryName","value":"IMS"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11799","properties":[{"key":"EntryName","value":"IMS_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF21999","properties":[{"key":"EntryName","value":"IMS_HHH_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF14377","properties":[{"key":"EntryName","value":"UBM"},{"key":"MatchStatus","value":"2"}]},{"database":"PIRSF","id":"PIRSF036603","properties":[{"key":"EntryName","value":"DPol_eta"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56672","properties":[{"key":"EntryName","value":"DNA/RNA polymerases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF100879","properties":[{"key":"EntryName","value":"Lesion bypass DNA polymerase (Y-family), little finger domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS52064","properties":[{"key":"EntryName","value":"UBM"},{"key":"MatchStatus","value":"2"}]},{"database":"PROSITE","id":"PS50173","properties":[{"key":"EntryName","value":"UMUC"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEPLHAGAAGSSRAVCSQGPPTQISSSRVIVHVDLDCFYAQVEMISNPELKDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKRLQQLPSEEVPSVTVFGHVYNNQSVNLHNIMHRRLVVGSQIAAEMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIPGIGYKTAKRLEVLGINSVHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEVEAKAKIEELLSSLLTRVCQDGRKPHTVRLVIRRYSDKHCNRESRQCPIPSHVIQKLGTGNHDSMPPLIDILMKLFRNMVNVKMPFHLTLMSVCFCNLKALSSAKKGPMDCYLTSLSTPAYTDKRAFKVKDTHTEDSHKEKEANWDCLPSRRIESTGTGESPLDATCFPKEKDTSDLPLQALPEGVDQEVFKQLPADIQEEILYGKSRENLKGKGSLSCPLHASRGVLSFFSTKQMQASRLSPRDTALPSKRVSAASPCEPGTSGLSPGSTSHPSCGKDCSYYIDSQLKDEQTSQGPTESQGCQFSSTNPAVSGFHSFPNLQTEQLFSTHRTVDSHKQTATASHQGLESHQGLESRELDSAEEKLPFPPDIDPQVFYELPEEVQKELMAEWERAGAARPSAHR","length":717,"molWeight":79653,"crc64":"43E5D2D0ECB3AA85","md5":"F33181A3450F7618DAD0AA96D6F5DF9B"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"TISSUE SPECIFICITY":1,"DOMAIN":2,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Domain":3,"Region":6,"Motif":2,"Compositional bias":4,"Active site":1,"Binding site":5,"Alternative sequence":1,"Natural variant":10,"Mutagenesis":6,"Sequence conflict":1,"Helix":5,"Turn":1,"Beta strand":1},"uniParcId":"UPI0000351979"}}