{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q6PDM4","uniProtkbId":"IHO1_MOUSE","entryAudit":{"firstPublicDate":"2007-09-11","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2010-11-30","entryVersion":110,"sequenceVersion":3},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27723721"}],"value":"Interactor of HORMAD1 protein 1"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:3612242"}],"value":"Coiled-coil domain-containing protein 36"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27723721"},{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:3612242"}],"value":"Iho1"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:3612242"}],"value":"Ccdc36"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27723721"}],"value":"Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination (PubMed:27723721). Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis (PubMed:27723721). Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance (PubMed:27723721)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27723721"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30569039"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31000436"}],"value":"Part of the MCD recombinosome complex, at least composed of IHO1, REC114 and MEI4 (PubMed:27723721, PubMed:30569039). Interacts with REC114 (PubMed:27723721). Interacts with MEI4 (PubMed:30569039). Interacts with HORMAD1 (PubMed:27723721). Interacts with ANKRD31 (PubMed:31000436)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27723721"}],"value":"Specifically localizes to unsynapsed chromosomal regions during meiosis (PubMed:27723721). Appears on chromatin during preleptotene, when pre-meiotic replication occurs and axes have not yet developed (PubMed:27723721). Remains associated with unsynapsed axesn zygotene, when axes elongate and synapsis begins but disappears from synapsed axes (PubMed:27723721). Localization diverges in the two sexes beyond zygotene (PubMed:27723721). In spermatocytes, synapsis between the largely non-homologous X and Y chromosomes is mostly constrained to their short pseudoautosomal regions (PARs) (PubMed:27723721). At the zygotene-to-pachytene transition, high levels remain on unsynapsed sex chromosome axes but also on PARs (PubMed:27723721). However, by early pachytene disappears from both the synapsed and unsynapsed regions of sex chromosomes (PubMed:27723721). Reaccumulates on unsynapsed axes of sex chromosomes in mid-late pachytene and on desynapsing autosome axes in diplotene, but disappears from chromatin concomitant with axis disassembly after diplotene (PubMed:27723721). In oocytes, disappears from axes after all chromosomes synapsed and is undetectable on chromatin beyond zygotene (PubMed:27723721). Ovarian levels also dramatically decrease as oocytes progress to late prophase (PubMed:27723721). HORMAD1 is required for robust accumulation on chromatin and unsynapsed axes, suggesting that HORMAD1 recruits IHO1 to unsynapsed axes (PubMed:27723721)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27723721"}],"value":"Chromosome","id":"SL-0468"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27723721"}],"value":"Detected in spermatocytes and testis (at protein level) (PubMed:27723721)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"27723721"}],"value":"Meiosis-specific"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27723721"}],"value":"Mice develop normally without obvious somatic defects but males and females are sterile due to defects in homologous synapsis (PubMed:27723721). Oocytes are depleted in six-week-old females and spermatocytes in males undergo apoptosis at a stage equivalent to wild-type mid-pachytene (PubMed:27723721). Complete synapsis is never observed and incomplete synaptonemal complexes are detected in meiocytes equivalent to late-zygotene and pachytene (PubMed:27723721)"}],"commentType":"DISRUPTION PHENOTYPE"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAH58624.1","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":574,"modifier":"EXACT"}},"description":"Interactor of HORMAD1 protein 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