{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q6NQK9","secondaryAccessions":["Q9XIE5"],"uniProtkbId":"CCB4_ARATH","entryAudit":{"firstPublicDate":"2015-06-24","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":118,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Protein COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B CCB4, chloroplastic"}},"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"18593701"}],"value":"CCB4"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"Araport","id":"AT1G59840"}],"value":"At1g59840"}],"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"AAD39336.1"}],"value":"F23H11.16"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18593701"}],"value":"Required for the biogenesis and accumulation of native cytochrome b6 in the thylakoid membrane. Controls the conversion of apocytochrome b6 to holocytochrome b6. Required for covalent binding of the c-type heme to cytochrome b6"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18593701"}],"value":"Plastid, chloroplast thylakoid membrane","id":"SL-0058"},"topology":{"evidences":[{"evidenceCode":"ECO:0000255"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18593701"}],"value":"Seedling lethal when grown on soil. On agar plates supplied with sucrose, seedlings grow very slowly with a chlorotic phenotype. Deficiency in the accumulation of the subunits of the cytochrome b6f complex and lack of covalent heme binding to cytochrome b6"}],"commentType":"DISRUPTION PHENOTYPE"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"AAD39336.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":33,"modifier":"EXACT"}},"description":"Chloroplast","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Chain","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"Protein COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B CCB4, chloroplastic","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000433264"},{"type":"Topological domain","location":{"start":{"value":34,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"Stromal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18593701"}]},{"type":"Transmembrane","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":92,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18593701"}]},{"type":"Transmembrane","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":124,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":297,"modifier":"EXACT"}},"description":"Stromal","evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"18593701"}]}],"keywords":[{"id":"KW-0150","category":"Cellular component","name":"Chloroplast"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0934","category":"Cellular component","name":"Plastid"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0793","category":"Cellular component","name":"Thylakoid"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14593172","citationType":"journal article","authors":["Yamada K.","Lim J.","Dale J.M.","Chen H.","Shinn P.","Palm C.J.","Southwick A.M.","Wu H.C.","Kim C.J.","Nguyen M.","Pham P.K.","Cheuk R.F.","Karlin-Newmann G.","Liu S.X.","Lam B.","Sakano H.","Wu T.","Yu G.","Miranda M.","Quach H.L.","Tripp M.","Chang C.H.","Lee J.M.","Toriumi M.J.","Chan M.M.","Tang C.C.","Onodera C.S.","Deng J.M.","Akiyama K.","Ansari Y.","Arakawa T.","Banh J.","Banno F.","Bowser L.","Brooks S.Y.","Carninci P.","Chao Q.","Choy N.","Enju A.","Goldsmith A.D.","Gurjal M.","Hansen N.F.","Hayashizaki Y.","Johnson-Hopson C.","Hsuan V.W.","Iida K.","Karnes M.","Khan S.","Koesema E.","Ishida J.","Jiang P.X.","Jones T.","Kawai J.","Kamiya A.","Meyers C.","Nakajima M.","Narusaka M.","Seki M.","Sakurai T.","Satou M.","Tamse R.","Vaysberg M.","Wallender E.K.","Wong C.","Yamamura Y.","Yuan S.","Shinozaki K.","Davis R.W.","Theologis A.","Ecker J.R."],"citationCrossReferences":[{"database":"PubMed","id":"14593172"},{"database":"DOI","id":"10.1126/science.1088305"}],"title":"Empirical analysis of transcriptional activity in the Arabidopsis genome.","publicationDate":"2003","journal":"Science","firstPage":"842","lastPage":"846","volume":"302"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-B9D7AITG0S7MM","citationType":"submission","authors":["Totoki Y.","Seki M.","Ishida J.","Nakajima M.","Enju A.","Kamiya A.","Narusaka M.","Shin-i T.","Nakagawa M.","Sakamoto N.","Oishi K.","Kohara Y.","Kobayashi M.","Toyoda A.","Sakaki Y.","Sakurai T.","Iida K.","Akiyama K.","Satou M.","Toyoda T.","Konagaya A.","Carninci P.","Kawai J.","Hayashizaki Y.","Shinozaki K."],"title":"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.","publicationDate":"MAR-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"18593701","citationType":"journal article","authors":["Lezhneva L.","Kuras R.","Ephritikhine G.","de Vitry C."],"citationCrossReferences":[{"database":"PubMed","id":"18593701"},{"database":"DOI","id":"10.1074/jbc.m803869200"}],"title":"A novel pathway of cytochrome c biogenesis is involved in the assembly of the cytochrome b6f complex in arabidopsis chloroplasts.","publicationDate":"2008","journal":"J. Biol. Chem.","firstPage":"24608","lastPage":"24616","volume":"283"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","TOPOLOGY","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC007258","properties":[{"key":"ProteinId","value":"AAD39336.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE33626.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE33627.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7031466.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BT010443","properties":[{"key":"ProteinId","value":"AAQ62444.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK175262","properties":[{"key":"ProteinId","value":"BAD43025.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK175368","properties":[{"key":"ProteinId","value":"BAD43131.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK221539","properties":[{"key":"ProteinId","value":"BAD94887.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"PIR","id":"E96622","properties":[{"key":"EntryName","value":"E96622"}]},{"database":"RefSeq","id":"NP_176193.2","properties":[{"key":"NucleotideSequenceId","value":"NM_104677.5"}]},{"database":"RefSeq","id":"NP_974051.1","properties":[{"key":"NucleotideSequenceId","value":"NM_202322.2"}]},{"database":"AlphaFoldDB","id":"Q6NQK9","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q6NQK9","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q6NQK9","properties":[{"key":"Number of interactors","value":"993"}]},{"database":"IntAct","id":"Q6NQK9","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"3702.Q6NQK9","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q6NQK9","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"222805","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"842278","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G59840","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G59840","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G59840","properties":[{"key":"GeneName","value":"CCB4"}]},{"database":"eggNOG","id":"ENOG502QSXP","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_082867_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q6NQK9","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"QGVICQP","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"PhylomeDB","id":"Q6NQK9","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q6NQK9","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"Q6NQK9","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0009507","properties":[{"key":"GoTerm","value":"C:chloroplast"},{"key":"GoEvidenceType","value":"IDA:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"18593701"}]},{"database":"GO","id":"GO:0009535","properties":[{"key":"GoTerm","value":"C:chloroplast thylakoid membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0010190","properties":[{"key":"GoTerm","value":"P:cytochrome b6f complex assembly"},{"key":"GoEvidenceType","value":"IMP:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"18593701"}]},{"database":"InterPro","id":"IPR021325","properties":[{"key":"EntryName","value":"CCB2/CCB4"}]},{"database":"InterPro","id":"IPR044705","properties":[{"key":"EntryName","value":"CCB4"}]},{"database":"PANTHER","id":"PTHR34943","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34943:SF2","properties":[{"key":"EntryName","value":"PROTEIN COFACTOR ASSEMBLY OF COMPLEX C SUBUNIT B CCB4, CHLOROPLASTIC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11152","properties":[{"key":"EntryName","value":"CCB2_CCB4"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MEARIILLRIQIPWSANRQFSHPPLDFPRFIRASSSSTSQKPKTYEGPKPRKNLVADFISKNDDLVRSLPIYVGGASLLAVLFNRTVSGIAPVADASSSQSRADLLALGLAVTNLLTGLVWLSIRPKSITPVNPKGVECKVVESDLPASMVSELLWAWESLKVATCCKSLVIVYNGICLIQIGMVAESPEDKKTVIVKTDKLMQGSVYRGVMKSKAQSYLANLSLYPGRSELPFLPANTQAVILQPLGDKGIAVIGGNTIRGFTSSDQAWISSIGEKLDATLGRYFVDSDEISRVTV","length":297,"molWeight":32222,"crc64":"E07455D51B19E5A7","md5":"D8050E6415156377928934C908AC4517"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Topological domain":3,"Transmembrane":2},"uniParcId":"UPI00001BD856"}}