{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q6LX54","uniProtkbId":"HCGC_METMP","entryAudit":{"firstPublicDate":"2026-06-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-07-05","entryVersion":77,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Methanococcus maripaludis (strain DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL)","taxonId":267377,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Methanomada group","Methanococci","Methanococcales","Methanococcaceae","Methanococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"6-carboxymethyl-5-methyl-4-hydroxypyridin-2-ol 3-C-methyltransferase HcgC"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27391308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28294213"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35286742"}],"value":"2.1.1.400"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27391308"}],"value":"SAM-dependent pyridinol methyltransferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27391308"}],"value":"hcgC"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CAF31054.1"}],"value":"MMP1498"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27391308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28294213"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35286742"}],"value":"SAM-dependent methyltransferase involved in the biosynthesis of the iron-guanylylpyridinol (FeGP) cofactor used by [Fe]-hydrogenase (PubMed:27391308, PubMed:28294213, PubMed:35286742). It catalyzes the transfer of a methyl group from S-adenosylmethionine to 6-carboxymethyl-5-methyl-4-hydroxy-2-pyridinol to form 6-carboxymethyl-3,5-dimethyl-4-hydroxy-2-pyridinol (PubMed:27391308, PubMed:28294213, PubMed:28682478, PubMed:35286742). It is the product of one of the 7 hmd co-occurring genes (hcg), hcgA-G, which cluster with the [Fe]-hydrogenase-encoding gene hmd and are required for the biosynthesis of the FeGP cofactor (PubMed:27391308)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"6-carboxymethyl-5-methyl-4-hydroxypyridin-2-ol + S-adenosyl-L-methionine = 6-carboxymethyl-3,5-dimethyl-4-hydroxypyridin-2-ol + S-adenosyl-L-homocysteine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:85483"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:234380"},{"database":"ChEBI","id":"CHEBI:234381"}],"ecNumber":"2.1.1.400","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27391308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28294213"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35286742"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:85484"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27391308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28294213"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35286742"}]}]},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":7.3,"unit":"uM","substrate":"6-carboxymethyl-5-methyl-4-hydroxypyridin-2-ol","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27391308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}],"value":"kcat is 2.5 min(-1) (PubMed:27391308). kcat is 2.4 min(-1) (PubMed:28682478)."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27391308"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28294213"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"35286742"}],"value":"Cofactor biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}],"value":"Homotetramer (PubMed:28682478). Dimer of dimers (PubMed:28682478)"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27391308"}],"value":"The mutant lacking this gene does not show [Fe]-hydrogenase activity"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the HcgC family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"6-carboxymethyl-5-methyl-4-hydroxypyridin-2-ol 3-C-methyltransferase HcgC","featureId":"PRO_0000463912"},{"type":"Binding site","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57856"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"S-adenosyl-L-homocysteine","id":"ChEBI:CHEBI:57856"}},{"type":"Binding site","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57856"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"S-adenosyl-L-homocysteine","id":"ChEBI:CHEBI:57856"}},{"type":"Binding site","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57856"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"S-adenosyl-L-homocysteine","id":"ChEBI:CHEBI:57856"}},{"type":"Binding site","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":53,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57856"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"S-adenosyl-L-homocysteine","id":"ChEBI:CHEBI:57856"}},{"type":"Binding site","location":{"start":{"value":72,"modifier":"EXACT"},"end":{"value":72,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57856"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"S-adenosyl-L-homocysteine","id":"ChEBI:CHEBI:57856"}},{"type":"Binding site","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":112,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57856"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"S-adenosyl-L-homocysteine","id":"ChEBI:CHEBI:57856"}},{"type":"Binding site","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:234380"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"6-carboxymethyl-5-methyl-4-hydroxypyridin-2-ol","id":"ChEBI:CHEBI:234380"}},{"type":"Binding site","location":{"start":{"value":134,"modifier":"EXACT"},"end":{"value":134,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57856"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"S-adenosyl-L-homocysteine","id":"ChEBI:CHEBI:57856"}},{"type":"Binding site","location":{"start":{"value":177,"modifier":"EXACT"},"end":{"value":177,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:57856"}],"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"S-adenosyl-L-homocysteine","id":"ChEBI:CHEBI:57856"}},{"type":"Binding site","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:234380"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}],"ligand":{"name":"6-carboxymethyl-5-methyl-4-hydroxypyridin-2-ol","id":"ChEBI:CHEBI:234380"}},{"type":"Mutagenesis","location":{"start":{"value":6,"modifier":"EXACT"},"end":{"value":6,"modifier":"EXACT"}},"description":"100-fold decrease in catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["V"]}},{"type":"Mutagenesis","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"7-fold decrease in catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}],"alternativeSequence":{"originalSequence":"Y","alternativeSequences":["F"]}},{"type":"Mutagenesis","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"2-fold decrease in catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":179,"modifier":"EXACT"},"end":{"value":179,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["V"]}},{"type":"Mutagenesis","location":{"start":{"value":209,"modifier":"EXACT"},"end":{"value":209,"modifier":"EXACT"}},"description":"Loss of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}],"alternativeSequence":{"originalSequence":"E","alternativeSequences":["Q"]}},{"type":"Mutagenesis","location":{"start":{"value":233,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"35-fold decrease in catalytic efficiency.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"28682478"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"15466049","citationType":"journal article","authors":["Hendrickson E.L.","Kaul R.","Zhou Y.","Bovee D.","Chapman P.","Chung J.","Conway de Macario E.","Dodsworth J.A.","Gillett W.","Graham D.E.","Hackett M.","Haydock A.K.","Kang A.","Land M.L.","Levy R.","Lie T.J.","Major T.A.","Moore B.C.","Porat I.","Palmeiri A.","Rouse G.","Saenphimmachak C.","Soell D.","Van Dien S.","Wang T.","Whitman W.B.","Xia Q.","Zhang Y.","Larimer F.W.","Olson M.V.","Leigh J.A."],"citationCrossReferences":[{"database":"PubMed","id":"15466049"},{"database":"DOI","id":"10.1128/jb.186.20.6956-6969.2004"}],"title":"Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis.","publicationDate":"2004","journal":"J. Bacteriol.","firstPage":"6956","lastPage":"6969","volume":"186"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"CAF31054.1"}]},{"referenceNumber":2,"citation":{"id":"27391308","citationType":"journal article","authors":["Fujishiro T.","Bai L.","Xu T.","Xie X.","Schick M.","Kahnt J.","Rother M.","Hu X.","Ermler U.","Shima S."],"citationCrossReferences":[{"database":"PubMed","id":"27391308"},{"database":"DOI","id":"10.1002/anie.201604352"}],"title":"Identification of HcgC as a SAM-Dependent Pyridinol Methyltransferase in [Fe]-Hydrogenase Cofactor Biosynthesis.","publicationDate":"2016","journal":"Angew. Chem. Int. Ed.","firstPage":"9648","lastPage":"9651","volume":"55"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","PATHWAY","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"28294213","citationType":"journal article","authors":["Bai L.","Fujishiro T.","Huang G.","Koch J.","Takabayashi A.","Yokono M.","Tanaka A.","Xu T.","Hu X.","Ermler U.","Shima S."],"citationCrossReferences":[{"database":"PubMed","id":"28294213"},{"database":"DOI","id":"10.1039/c6fd00209a"}],"title":"Towards artificial methanogenesis: biosynthesis of the [Fe]-hydrogenase cofactor and characterization of the semi-synthetic hydrogenase.","publicationDate":"2017","journal":"Faraday Discuss.","firstPage":"37","lastPage":"58","volume":"198"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","PATHWAY"],"referenceComments":[{"value":"DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"35286742","citationType":"journal article","authors":["Schaupp S.","Arriaza-Gallardo F.J.","Pan H.J.","Kahnt J.","Angelidou G.","Paczia N.","Costa K.","Hu X.","Shima S."],"citationCrossReferences":[{"database":"PubMed","id":"35286742"},{"database":"DOI","id":"10.1002/anie.202200994"}],"title":"In Vitro Biosynthesis of the [Fe]-Hydrogenase Cofactor Verifies the Proposed Biosynthetic Precursors.","publicationDate":"2022","journal":"Angew. Chem. Int. Ed.","firstPage":"e202200994","lastPage":"e202200994","volume":"61"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","PATHWAY"]},{"referenceNumber":5,"citation":{"id":"28682478","citationType":"journal article","authors":["Bai L.","Wagner T.","Xu T.","Hu X.","Ermler U.","Shima S."],"citationCrossReferences":[{"database":"PubMed","id":"28682478"},{"database":"DOI","id":"10.1002/anie.201705605"}],"title":"A Water-Bridged H-Bonding Network Contributes to the Catalysis of the SAM-Dependent C-Methyltransferase HcgC.","publicationDate":"2017","journal":"Angew. Chem. Int. Ed.","firstPage":"10806","lastPage":"10809","volume":"56"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH 6-CARBOXYMETHYL-5-METHYL-4-HYDROXYPYRIDIN-2-OL AND S-ADENOSYL-L-HOMOCYSTEINE","FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT","MUTAGENESIS OF THR-6; TYR-51; SER-175; THR-179; GLU-209 AND SER-233"],"evidences":[{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4H"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4J"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4M"},{"evidenceCode":"ECO:0007744","source":"PDB","id":"5O4N"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"BX950229","properties":[{"key":"ProteinId","value":"CAF31054.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_011171442.1","properties":[{"key":"NucleotideSequenceId","value":"NC_005791.1"}]},{"database":"PDB","id":"5O4H","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.75 A"},{"key":"Chains","value":"A/B/C/D=1-260"}]},{"database":"PDB","id":"5O4J","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.70 A"},{"key":"Chains","value":"A/B/C/D=1-260"}]},{"database":"PDB","id":"5O4M","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.10 A"},{"key":"Chains","value":"A/B/C/D=1-260"}]},{"database":"PDB","id":"5O4N","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.05 A"},{"key":"Chains","value":"A/B/C/D=1-260"}]},{"database":"PDBsum","id":"5O4H","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5O4J","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5O4M","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5O4N","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q6LX54","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q6LX54","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"267377.MMP1498","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"CAF31054","properties":[{"key":"ProteinId","value":"CAF31054"},{"key":"GeneId","value":"MMP1498"}]},{"database":"GeneID","id":"2762530","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmp:MMP1498","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|267377.15.peg.1535","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"eggNOG","id":"arCOG04864","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_081185_0_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"114526at2157","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.1.1.400","properties":[{"key":"OrganismId","value":"3262"}]},{"database":"Proteomes","id":"UP000000590","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"InterPro","id":"IPR009573","properties":[{"key":"EntryName","value":"HcgC"}]},{"database":"Pfam","id":"PF06690","properties":[{"key":"EntryName","value":"HcgC"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF019265","properties":[{"key":"EntryName","value":"DUF1188"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MNYGITESVKTTRSKIKIKDIVSDVVEKKANAIKYFLEGEEFKQAIVFGAYLSGSYIAYSLLKDCEEVIIVDIQPHLKDILFNDGIKFMDLNKLQLELRNGTSINPDLVIDLTGIGGVSPDLISKFNPKVLIVEDPKGNHDKGISKIDNTDKRLCVGAKKGVLKTYRSSKFSKTSGTMTLVVDIIMDSCREINELDSVLYTIPNLKYFEGTVFHEKNVKKFLTELNMSAITVSSIDHVEYELEEILSKNISRVDSFVKEF","length":260,"molWeight":29262,"crc64":"B5402702A6B0CD0A","md5":"D1A2B52D23A3B5EB0DC7000BA58F721D"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":10,"Mutagenesis":6},"uniParcId":"UPI0000242A8A"}}