{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q6DRC3","secondaryAccessions":["Q7T371"],"uniProtkbId":"CF298_DANRE","entryAudit":{"firstPublicDate":"2009-01-20","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2004-08-16","entryVersion":93,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Danio rerio","commonName":"Zebrafish","synonyms":["Brachydanio rerio"],"taxonId":7955,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Actinopterygii","Neopterygii","Teleostei","Ostariophysi","Cypriniformes","Danionidae","Danioninae","Danio"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cilia- and flagella-associated protein 298"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26904945"}],"value":"Protein kurly"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P57076"}],"value":"cfap298"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"26904945"}],"value":"kur"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24094744"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26904945"}],"value":"Plays a role in motile cilium function, possibly by acting on outer dynein arm assembly (PubMed:24094744, PubMed:26904945). Seems to be important for initiation rather than maintenance of cilium motility (PubMed:26904945). Required for correct positioning of cilia at the apical cell surface, suggesting an additional role in the planar cell polarity (PCP) pathway (PubMed:26904945). May suppress canonical Wnt signaling activity (PubMed:26904945)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26904945"}],"value":"Interacts with dnaaf1/swt. Interacts with lrrc6/sea. Interacts with dvl (via DEP and PDZ domains)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26904945"}],"value":"Appears in cytoplasmic puncta, compatible with a centrosomal localization"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26904945"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A1L8HCK2"}],"value":"Cytoplasm, cytoskeleton, cilium basal body","id":"SL-0087"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24094744"}],"value":"Strongly expressed in ciliated tissues of the embryonic trunk, including the pronephric ducts and spinal canal"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26904945"}],"value":"Detected at the 1-cell embryo stage, probably due to inheritance of maternal transcripts. At the 50% ebiboly stage, detected in dorsal forerunner cells. At the 3-somite stage, found in Kupffer's vesicle. At the 8-somite stage, expressed in the pronephros, the floorplate of the neural tube, and otic vesicles. Expression in the pronephros continues to the 24-somite stage"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24094744"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26904945"}],"value":"Larval lethal (PubMed:26904945). Animals show strong ciliopathy phenotypes, including pronephric cysts, axis curvature, left-right asymmetry defects and hydrocephalus (PubMed:26904945). Kidney tubules are dilated and polarity of cilia in the epithelium is disorganized (PubMed:26904945). Cilia length and number appear normal, but outer dynein arms are missing and cilia are paralyzed (PubMed:26904945). Morpholino knockdown of the protein results in similar phenotypes (PubMed:24094744, PubMed:26904945)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CFAP298 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":290,"modifier":"EXACT"}},"description":"Cilia- and flagella-associated protein 298","featureId":"PRO_0000360153"},{"type":"Mutagenesis","location":{"start":{"value":44,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"In tm304; temperature sensitive allele with strongest phenotypes at 32 degrees Celsius and milder phenotypes at 25 degrees Celsius. Body curvature defects and kidney cysts are seen in larval stages. Left-right asymmetry is abnormal with reversed or random positioning of heart, liver and pancreas. Males are infertile. Cilium length and number are unaffected. Initiation of cilium motility is impaired, and cilium outer dynein arms are absent. Cilium polarity is disorganized.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"26904945"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["N"]}},{"type":"Sequence conflict","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"in Ref. 2; AAH53230","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["S"]}}],"keywords":[{"id":"KW-0966","category":"Cellular component","name":"Cell projection"},{"id":"KW-0969","category":"Cellular component","name":"Cilium"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0206","category":"Cellular component","name":"Cytoskeleton"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"15256591","citationType":"journal article","authors":["Amsterdam A.","Nissen R.M.","Sun Z.","Swindell E.C.","Farrington S.","Hopkins N."],"citationCrossReferences":[{"database":"PubMed","id":"15256591"},{"database":"DOI","id":"10.1073/pnas.0403929101"}],"title":"Identification of 315 genes essential for early zebrafish development.","publicationDate":"2004","journal":"Proc. 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