{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q69ZS7","secondaryAccessions":["Q91Z32","Q9CVT2","Q9CZ95","Q9WTY5"],"uniProtkbId":"HBS1L_MOUSE","entryAudit":{"firstPublicDate":"2005-05-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-05-10","entryVersion":171,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"HBS1-like protein"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P32769"}],"value":"3.6.5.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"33899734"}],"value":"Hbs1l"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9872408"}],"value":"Hbs1"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"15368895"}],"value":"Kiaa1038"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}],"value":"GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"GTP + H2O = GDP + phosphate + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:19669"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:58189"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P32769"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:19670"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P32769"}]}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}],"value":"Component of the Pelota-HBS1L complex, also named Dom34-Hbs1 complex, composed of PELO and HBS1L. Interacts with the SKI complex"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q69ZS7","intActId":"EBI-16114976"},"interactantTwo":{"uniProtKBAccession":"Q80X73","geneName":"Pelo","intActId":"EBI-16114899"},"numberOfExperiments":2,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}],"value":"Cytoplasm","id":"SL-0086"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q69ZS7-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q69ZS7-2"],"sequenceIds":["VSP_013626"],"isoformSequenceStatus":"Described"},{"name":{"value":"3"},"isoformIds":["Q69ZS7-3"],"sequenceIds":["VSP_013627","VSP_013628"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"9872408"}],"value":"Detected in embryos"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"33899734"}],"value":"Embryonic lethality; embryos fail to develop after 8.5 dpc (PubMed:33899734). Conditional deletion in the in the developing cerebellum and midbrain leads to severe development defects in the cerebellum characterized by the absence of multiple cell types (PubMed:33899734). In contrast, conditional deletion in the adult cerebellum and midbrain does not cause defects in neuron survival (PubMed:33899734). Defects are probably caused by ribosome pausing, due to inability to rescue stalled ribosomes (PubMed:33899734)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01059"}],"value":"Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAD32369.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":682,"modifier":"EXACT"}},"description":"HBS1-like protein","featureId":"PRO_0000091492"},{"type":"Domain","location":{"start":{"value":256,"modifier":"EXACT"},"end":{"value":480,"modifier":"EXACT"}},"description":"tr-type G","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":239,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"G1","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":321,"modifier":"EXACT"},"end":{"value":325,"modifier":"EXACT"}},"description":"G2","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":342,"modifier":"EXACT"},"end":{"value":345,"modifier":"EXACT"}},"description":"G3","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":404,"modifier":"EXACT"},"end":{"value":407,"modifier":"EXACT"}},"description":"G4","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Region","location":{"start":{"value":443,"modifier":"EXACT"},"end":{"value":445,"modifier":"EXACT"}},"description":"G5","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU01059"}]},{"type":"Compositional bias","location":{"start":{"value":142,"modifier":"EXACT"},"end":{"value":154,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":220,"modifier":"EXACT"},"end":{"value":231,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Binding site","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":404,"modifier":"EXACT"},"end":{"value":407,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Binding site","location":{"start":{"value":443,"modifier":"EXACT"},"end":{"value":445,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:37565"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}],"ligand":{"name":"GTP","id":"ChEBI:CHEBI:37565"}},{"type":"Modified residue","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}]},{"type":"Modified residue","location":{"start":{"value":113,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}]},{"type":"Modified residue","location":{"start":{"value":148,"modifier":"EXACT"},"end":{"value":148,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":150,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"21183079"}]},{"type":"Modified residue","location":{"start":{"value":229,"modifier":"EXACT"},"end":{"value":229,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9Y450"}]},{"type":"Modified residue","location":{"start":{"value":620,"modifier":"EXACT"},"end":{"value":620,"modifier":"EXACT"}},"description":"N6-acetyllysine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"23806337"}]},{"type":"Alternative sequence","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"9872408"}],"featureId":"VSP_013626","alternativeSequence":{}},{"type":"Alternative sequence","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16141072"}],"featureId":"VSP_013627","alternativeSequence":{"originalSequence":"KSVISRSSQSESEIVPKVAKMTVSGKK","alternativeSequences":["VLFSSFGVSPQNVHHSYLQSENHLDSS"]}},{"type":"Alternative sequence","location":{"start":{"value":168,"modifier":"EXACT"},"end":{"value":682,"modifier":"EXACT"}},"description":"in isoform 3","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16141072"}],"featureId":"VSP_013628","alternativeSequence":{}},{"type":"Sequence conflict","location":{"start":{"value":201,"modifier":"EXACT"},"end":{"value":201,"modifier":"EXACT"}},"description":"in Ref. 1; AAD23351","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"A","alternativeSequences":["R"]}},{"type":"Sequence conflict","location":{"start":{"value":251,"modifier":"EXACT"},"end":{"value":252,"modifier":"EXACT"}},"description":"in Ref. 1; AAD23351","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"KR","alternativeSequences":["NAV"]}},{"type":"Sequence conflict","location":{"start":{"value":568,"modifier":"EXACT"},"end":{"value":568,"modifier":"EXACT"}},"description":"in Ref. 3; AAH10251","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["A"]}},{"type":"Helix","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":57,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UFZ"}]},{"type":"Helix","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":68,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UFZ"}]},{"type":"Helix","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UFZ"}]},{"type":"Helix","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":89,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UFZ"}]},{"type":"Helix","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":103,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UFZ"}]},{"type":"Helix","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"1UFZ"}]},{"type":"Sequence conflict","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"},"sequence":"Q69ZS7-3"},"description":"in Ref. 3; BAB24679","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"F","alternativeSequences":["L"]}}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0251","category":"Molecular function","name":"Elongation factor"},{"id":"KW-0342","category":"Ligand","name":"GTP-binding"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0648","category":"Biological process","name":"Protein biosynthesis"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0810","category":"Biological process","name":"Translation regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"9872408","citationType":"journal article","authors":["Wallrapp C.","Verrier S.-B.","Zhouravleva G.","Philippe H.","Philippe M.","Gress T.M.","Jean-Jean O."],"citationCrossReferences":[{"database":"PubMed","id":"9872408"},{"database":"DOI","id":"10.1016/s0014-5793(98)01492-6"}],"title":"The product of the mammalian orthologue of the Saccharomyces cerevisiae HBS1 gene is phylogenetically related to eukaryotic release factor 3 (eRF3) but does not carry eRF3-like activity.","publicationDate":"1998","journal":"FEBS Lett.","firstPage":"387","lastPage":"392","volume":"440"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)","TISSUE SPECIFICITY"],"referenceComments":[{"value":"NIH Swiss","type":"STRAIN"},{"value":"Embryo","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15368895","citationType":"journal article","authors":["Okazaki N.","Kikuno R.","Ohara R.","Inamoto S.","Koseki H.","Hiraoka S.","Saga Y.","Seino S.","Nishimura M.","Kaisho T.","Hoshino K.","Kitamura H.","Nagase T.","Ohara O.","Koga H."],"citationCrossReferences":[{"database":"PubMed","id":"15368895"},{"database":"DOI","id":"10.1093/dnares/11.3.205"}],"title":"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.","publicationDate":"2004","journal":"DNA Res.","firstPage":"205","lastPage":"218","volume":"11"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"Pancreatic islet","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Embryo","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"FVB/N","type":"STRAIN"},{"value":"Mammary gland","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-148 AND SER-150","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Brain","type":"TISSUE"},{"value":"Brown adipose tissue","type":"TISSUE"},{"value":"Heart","type":"TISSUE"},{"value":"Liver","type":"TISSUE"},{"value":"Lung","type":"TISSUE"},{"value":"Pancreas","type":"TISSUE"},{"value":"Spleen","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":6,"citation":{"id":"23806337","citationType":"journal article","authors":["Park J.","Chen Y.","Tishkoff D.X.","Peng C.","Tan M.","Dai L.","Xie Z.","Zhang Y.","Zwaans B.M.","Skinner M.E.","Lombard D.B.","Zhao Y."],"citationCrossReferences":[{"database":"PubMed","id":"23806337"},{"database":"DOI","id":"10.1016/j.molcel.2013.06.001"}],"title":"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.","publicationDate":"2013","journal":"Mol. Cell","firstPage":"919","lastPage":"930","volume":"50"},"referencePositions":["ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-620","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Embryonic fibroblast","type":"TISSUE"}]},{"referenceNumber":7,"citation":{"id":"33899734","citationType":"journal article","authors":["Terrey M.","Adamson S.I.","Chuang J.H.","Ackerman S.L."],"citationCrossReferences":[{"database":"PubMed","id":"33899734"},{"database":"DOI","id":"10.7554/elife.66904"}],"title":"Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology.","publicationDate":"2021","journal":"Elife","firstPage":"0","lastPage":"0","volume":"10"},"referencePositions":["DISRUPTION PHENOTYPE"]},{"referenceNumber":8,"citation":{"id":"CI-8E6JNSL6H7VAS","citationType":"submission","authoringGroup":["RIKEN structural genomics initiative (RSGI)"],"title":"Solution structure of HBS1-like domain in hypothetical protein BAB28515.","publicationDate":"AUG-2004","submissionDatabase":"PDB data bank"},"referencePositions":["STRUCTURE BY NMR OF 51-120"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AF087672","properties":[{"key":"ProteinId","value":"AAD23351.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK173091","properties":[{"key":"ProteinId","value":"BAD32369.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK006626","properties":[{"key":"ProteinId","value":"BAB24679.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK012856","properties":[{"key":"ProteinId","value":"BAB28515.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC010251","properties":[{"key":"ProteinId","value":"AAH10251.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS35862.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q69ZS7-1"},{"database":"RefSeq","id":"NP_062676.2","properties":[{"key":"NucleotideSequenceId","value":"NM_019702.3"}],"isoformId":"Q69ZS7-1"},{"database":"PDB","id":"1UFZ","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=51-120"}]},{"database":"PDB","id":"9WPR","properties":[{"key":"Method","value":"NMR"},{"key":"Resolution","value":"-"},{"key":"Chains","value":"A=51-120"}]},{"database":"PDBsum","id":"1UFZ","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"9WPR","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"207968","properties":[{"key":"Interactions","value":"6"}]},{"database":"DIP","id":"DIP-61684N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q69ZS7","properties":[{"key":"Number of interactors","value":"1615"}]},{"database":"IntAct","id":"Q69ZS7","properties":[{"key":"Interactions","value":"2"}]},{"database":"MINT","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"10090.ENSMUSP00000151689","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q69ZS7","properties":[{"key":"glycosylation","value":"3 sites, 1 N-linked glycan (1 site), 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000151689","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"270946","properties":[{"key":"Description","value":"-"}],"isoformId":"Q69ZS7-1"},{"database":"ProteomicsDB","id":"270947","properties":[{"key":"Description","value":"-"}],"isoformId":"Q69ZS7-2"},{"database":"ProteomicsDB","id":"270948","properties":[{"key":"Description","value":"-"}],"isoformId":"Q69ZS7-3"},{"database":"Pumba","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"32972","properties":[{"key":"antibodies","value":"395 antibodies from 27 providers"}]},{"database":"Ensembl","id":"ENSMUST00000219915.2","properties":[{"key":"ProteinId","value":"ENSMUSP00000151689.2"},{"key":"GeneId","value":"ENSMUSG00000019977.17"}],"isoformId":"Q69ZS7-1"},{"database":"GeneID","id":"56422","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:56422","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc007eom.1","properties":[{"key":"OrganismName","value":"mouse"}],"isoformId":"Q69ZS7-3"},{"database":"UCSC","id":"uc007eoo.1","properties":[{"key":"OrganismName","value":"mouse"}],"isoformId":"Q69ZS7-1"},{"database":"AGR","id":"MGI:1891704","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"10767","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1891704","properties":[{"key":"GeneName","value":"Hbs1l"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000019977","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0458","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000156274","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_007265_3_6_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"VVQITCH","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"342024at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-MMU-429958","properties":[{"key":"PathwayName","value":"mRNA decay by 3' to 5' exoribonuclease"}]},{"database":"Reactome","id":"R-MMU-9954714","properties":[{"key":"PathwayName","value":"PELO:HBS1L and ABCE1 dissociate a ribosome on a non-stop mRNA"}]},{"database":"BioGRID-ORCS","id":"56422","properties":[{"key":"hits","value":"19 hits in 76 CRISPR screens"}]},{"database":"EvolutionaryTrace","id":"Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q69ZS7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 10"}]},{"database":"RNAct","id":"Q69ZS7","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000019977","properties":[{"key":"ExpressionPatterns","value":"Expressed in spermatid and 266 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q69ZS7","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0022626","properties":[{"key":"GoTerm","value":"C:cytosolic ribosome"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:1990533","properties":[{"key":"GoTerm","value":"C:Dom34-Hbs1 complex"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005525","properties":[{"key":"GoTerm","value":"F:GTP binding"},{"key":"GoEvidenceType","value":"ISS:MGI"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"PubMed","id":"9872408"}]},{"database":"GO","id":"GO:0003924","properties":[{"key":"GoTerm","value":"F:GTPase activity"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0070966","properties":[{"key":"GoTerm","value":"P:nuclear-transcribed mRNA catabolic process, no-go decay"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0072344","properties":[{"key":"GoTerm","value":"P:rescue of stalled cytosolic ribosome"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0032790","properties":[{"key":"GoTerm","value":"P:ribosome disassembly"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0006412","properties":[{"key":"GoTerm","value":"P:translation"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"CDD","id":"cd01883","properties":[{"key":"EntryName","value":"EF1_alpha"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd16267","properties":[{"key":"EntryName","value":"HBS1-like_II"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd04093","properties":[{"key":"EntryName","value":"HBS1_C_III"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.8.10:FF:000039","properties":[{"key":"EntryName","value":"HBS1-like translational GTPase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.30.10:FF:000035","properties":[{"key":"EntryName","value":"HBS1-like translational GTPase"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.40.30.10:FF:000020","properties":[{"key":"EntryName","value":"Translation elongation factor EF-1"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"3.40.50.300:FF:000204","properties":[{"key":"EntryName","value":"Translation elongation factor Tu"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.8.10","properties":[{"key":"EntryName","value":"DNA helicase RuvA subunit, C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.300","properties":[{"key":"EntryName","value":"P-loop containing nucleotide triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.30.10","properties":[{"key":"EntryName","value":"Translation factors"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR004161","properties":[{"key":"EntryName","value":"EFTu-like_2"}]},{"database":"InterPro","id":"IPR054696","properties":[{"key":"EntryName","value":"GTP-eEF1A_C"}]},{"database":"InterPro","id":"IPR015033","properties":[{"key":"EntryName","value":"HBS1-like_N"}]},{"database":"InterPro","id":"IPR037189","properties":[{"key":"EntryName","value":"HBS1-like_N_sf"}]},{"database":"InterPro","id":"IPR027417","properties":[{"key":"EntryName","value":"P-loop_NTPase"}]},{"database":"InterPro","id":"IPR000795","properties":[{"key":"EntryName","value":"T_Tr_GTP-bd_dom"}]},{"database":"InterPro","id":"IPR050100","properties":[{"key":"EntryName","value":"TRAFAC_GTPase_members"}]},{"database":"InterPro","id":"IPR009000","properties":[{"key":"EntryName","value":"Transl_B-barrel_sf"}]},{"database":"InterPro","id":"IPR009001","properties":[{"key":"EntryName","value":"Transl_elong_EF1A/Init_IF2_C"}]},{"database":"PANTHER","id":"PTHR23115","properties":[{"key":"EntryName","value":"TRANSLATION FACTOR"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF22594","properties":[{"key":"EntryName","value":"GTP-eEF1A_C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00009","properties":[{"key":"EntryName","value":"GTP_EFTU"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03144","properties":[{"key":"EntryName","value":"GTP_EFTU_D2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF08938","properties":[{"key":"EntryName","value":"HBS1_N"},{"key":"MatchStatus","value":"1"}]},{"database":"PRINTS","id":"PR00315","properties":[{"key":"EntryName","value":"ELONGATNFCT"}]},{"database":"SUPFAM","id":"SSF50465","properties":[{"key":"EntryName","value":"EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF109732","properties":[{"key":"EntryName","value":"HBS1-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52540","properties":[{"key":"EntryName","value":"P-loop containing nucleoside triphosphate hydrolases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50447","properties":[{"key":"EntryName","value":"Translation proteins"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51722","properties":[{"key":"EntryName","value":"G_TR_2"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MARHRNVRGYNYDEDFEDDDLYGQSVEDDYCISPSTAAQFIYSRRDNPEEEYGYEDLRESSNSLLNHQLSEIDQARLYSCLDHMREVLGDAVPDDILTEAILKHKFDVQKALSVVLEQDGVQPWKEKSERAVCAGQPSKGKSVISRSSQSESEIVPKVAKMTVSGKKQTMGFEVPGLTSEENGLSVRAPHKGPPGDDVSVASPNIPETGTPKSALPPPSLQTSEELGSTPTPVRKSGKLRQQIDVKAELEKRQGGKQLLNLVVIGHVDAGKSTLMGHMLYLLGNVNKRTMHKYEQESKKAGKASFAYAWVLDETGEERERGVTMDVGMTKFETTTKVITLMDAPGHKDFIPNMITGAAQADVAVLVVDASRGEFEAGFETGGQTREHGLLVRSLGVTQLAVAVNKMDQVNWQQERFQEITGKLGHFLKQAGFKESDVAFIPTSGLSGENLTARSQSSDLTTWYKGMCLLEQIDSFKPPQRSIDKPFRLCVSDVFKDQGSGFCVTGKIEAGYIQTGDRLLAMPPNETCTAKGITLHDEPVDWAAAGDHVNLTLVGMDIIKINVGCIFCGPKEPIKACTRFRARILVFNIEVPITKGFPVLLHYQTVSEPAVIKRLISVLNKSTGEVTKKKPKLLTKGQNALVELQTQRPVALELYKDFKELGRFMLRYGGSTVAAGVVTEIKE","length":682,"molWeight":75100,"crc64":"53ADD45EC0872968","md5":"C721AB1760DA2BECB463D321A0D579C8"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":3,"TISSUE SPECIFICITY":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":7,"Compositional bias":3,"Binding site":3,"Modified residue":6,"Alternative sequence":3,"Sequence conflict":4,"Helix":6},"uniParcId":"UPI00001E4A7C"}}