{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q5MJS3","secondaryAccessions":["Q4FJP0","Q571A3","Q6PKA8","Q8JZP7"],"uniProtkbId":"FA20C_MOUSE","entryAudit":{"firstPublicDate":"2007-08-21","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-02-01","entryVersion":133,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Extracellular serine/threonine protein kinase FAM20C"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"value":"2.7.11.1"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17369251"}],"value":"Dentin matrix protein 4"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17369251"}],"value":"DMP-4"}]},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"Golgi-enriched fraction casein kinase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"GEF-CK"}]}],"flag":"Precursor"},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:2136853"}],"value":"Fam20c"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"17369251"}],"value":"Dmp4"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.2"}],"value":"Kiaa4081"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22732358"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25789606"}],"value":"Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth (PubMed:22732358, PubMed:22900076, PubMed:25789606). Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome (By similarity). Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity (By similarity). Phosphorylates ERO1A, enhancing its activity which is required to maintain endoplasmic reticulum redox homeostasis and for oxidative protein folding (By similarity). During endoplasmic reticulum stress, phosphorylates P4HB/PDIA1 which induces a functional switch, causing P4HB to change from an oxidoreductase to a molecular chaperone (By similarity). This is critical to maintain ER proteostasis and reduce cell death under ER stress (By similarity). Phosphorylation of P4HB also promotes its interaction with ERN1, leading to reduced activity of ERN1, a key sensor for the endoplasmic reticulum unfolded protein response (By similarity). Required for osteoblast differentiation and mineralization (By similarity). Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1 (PubMed:25789606). In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion (By similarity)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:17989"},{"database":"Rhea","id":"RHEA-COMP:9863"},{"database":"Rhea","id":"RHEA-COMP:11604"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:29999"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:83421"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.1","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}]}},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:46608"},{"database":"Rhea","id":"RHEA-COMP:11060"},{"database":"Rhea","id":"RHEA-COMP:11605"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:30013"},{"database":"ChEBI","id":"CHEBI:30616"},{"database":"ChEBI","id":"CHEBI:61977"},{"database":"ChEBI","id":"CHEBI:456216"}],"ecNumber":"2.7.11.1","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25789606"}],"value":"Serine/threonine protein kinase activity is increased upon interaction with FAM20A"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":0.7,"unit":"umol/min/mg","enzyme":"enzyme","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}]}],"michaelisConstants":[{"constant":1.5,"unit":"uM","substrate":"casein","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}]},{"constant":78.0,"unit":"uM","substrate":"ATP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"value":"kcat is 52 min(-1)."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25789606"}],"value":"Homodimer; disulfide-linked (By similarity). Interacts with FAM20A; probably forming a heterotetramer of 2 subunits of FAM20A and 2 subunits of FAM20C (PubMed:25789606). Interacts with COPII components SEC23A and SEC24A; transport of FAM20C from the endoplasmic reticulum to the Golgi is likely to be mediated by COPII vesicles (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"Resides in the Golgi apparatus membrane and is secreted following propeptide cleavage. Retained in the endoplasmic reticulum (ER) in response to ER stress where it phosphorylates P4HB"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"Golgi apparatus membrane","id":"SL-0134"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"Single-pass type II membrane protein","id":"SL-9906"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17369251"}],"value":"Secreted","id":"SL-0243"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"Endoplasmic reticulum","id":"SL-0095"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17369251"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"21549343"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29858230"}],"value":"In the mammary gland, expressed at higher levels in lactating mice than in virgin mice (at protein level) (PubMed:29858230). Highly expressed in the tooth. No expression in the dental pulp. At the secretory stage of amelogenesis, it is detected in the matrix of the enamel, in the ameloblasts, and within the cells adjoining the stratum intermedium (a tissue layer analogous to the stellate reticulum seen in the developing molar). Strong expression is observed in maturation stage ameloblasts and throughout the non-cornified layers of the gingival epithelium. Expressed at moderate levels in bone and at low levels in kidney, liver, brain and lung. Very low expression, if any, in spleen and skeletal muscle"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17369251"}],"value":"In the developing tooth, initial expression observed in odontoblasts at all stages of development. At later stages, restricted expression pattern in ameloblasts. Also observed in osteoblasts in the alveolar bone"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"N-glycosylation is required for folding"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"Autophosphorylated"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"value":"Propeptide cleavage by MBTPS1/S1P promotes FAM20C secretion and maximal kinase activity which is essential for efficient osteoblast differentiation and biomineralization"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22732358"}],"value":"Mice survive to adulthood and show biomineralization defects, suc as severe amelogenesis imperfecta (AI). In addition, mice are severely hypophosphatemic and develop notable lesions in both dentin and bone"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the FAM20 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAH04044.3","note":"Truncated N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"AAH04044.3","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAH25814.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAH25826.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAD90211.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"BAD90211.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Propeptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}],"featureId":"PRO_0000433884"},{"type":"Chain","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":579,"modifier":"EXACT"}},"description":"Extracellular serine/threonine protein kinase FAM20C","featureId":"PRO_0000297978"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Transmembrane","location":{"start":{"value":11,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"Helical; Signal-anchor for type II membrane protein","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":579,"modifier":"EXACT"}},"description":"Lumenal","evidences":[{"evidenceCode":"ECO:0000305"}]},{"type":"Region","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":79,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":349,"modifier":"EXACT"},"end":{"value":560,"modifier":"EXACT"}},"description":"Kinase domain","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}]},{"type":"Compositional bias","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":111,"modifier":"EXACT"},"end":{"value":120,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":128,"modifier":"EXACT"},"end":{"value":140,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":453,"modifier":"EXACT"},"end":{"value":453,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}]},{"type":"Binding site","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":280,"modifier":"EXACT"},"end":{"value":280,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":301,"modifier":"EXACT"},"end":{"value":301,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035"}},{"type":"Binding site","location":{"start":{"value":384,"modifier":"EXACT"},"end":{"value":387,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":458,"modifier":"EXACT"},"end":{"value":458,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":473,"modifier":"EXACT"},"end":{"value":473,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:30616"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}],"ligand":{"name":"ATP","id":"ChEBI:CHEBI:30616"}},{"type":"Binding site","location":{"start":{"value":473,"modifier":"EXACT"},"end":{"value":473,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035"}},{"type":"Site","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"Cleavage; by MBTPS1","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}]},{"type":"Glycosylation","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":""},{"type":"Disulfide bond","location":{"start":{"value":46,"modifier":"EXACT"},"end":{"value":46,"modifier":"EXACT"}},"description":"Interchain","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}]},{"type":"Disulfide bond","location":{"start":{"value":48,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"Interchain","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IXL6"}]},{"type":"Disulfide bond","location":{"start":{"value":357,"modifier":"EXACT"},"end":{"value":373,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}]},{"type":"Disulfide bond","location":{"start":{"value":362,"modifier":"EXACT"},"end":{"value":366,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}]},{"type":"Disulfide bond","location":{"start":{"value":421,"modifier":"EXACT"},"end":{"value":495,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}]},{"type":"Disulfide bond","location":{"start":{"value":496,"modifier":"EXACT"},"end":{"value":555,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9XTW2"}]},{"type":"Mutagenesis","location":{"start":{"value":241,"modifier":"EXACT"},"end":{"value":241,"modifier":"EXACT"}},"description":"Abrogates kinase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"alternativeSequence":{"originalSequence":"I","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":261,"modifier":"EXACT"},"end":{"value":261,"modifier":"EXACT"}},"description":"Abrogates kinase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":374,"modifier":"EXACT"},"end":{"value":374,"modifier":"EXACT"}},"description":"Abrogates kinase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"alternativeSequence":{"originalSequence":"G","alternativeSequences":["E","R"]}},{"type":"Mutagenesis","location":{"start":{"value":383,"modifier":"EXACT"},"end":{"value":383,"modifier":"EXACT"}},"description":"Mislocalization of the protein from Golgi apparatus to endoplasmic reticulum.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"alternativeSequence":{"originalSequence":"L","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":446,"modifier":"EXACT"},"end":{"value":446,"modifier":"EXACT"}},"description":"Mislocalization of the protein from Golgi apparatus to endoplasmic reticulum.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":453,"modifier":"EXACT"},"end":{"value":453,"modifier":"EXACT"}},"description":"Abrogates kinase activity without affecting subcellular location.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["G"]}},{"type":"Mutagenesis","location":{"start":{"value":473,"modifier":"EXACT"},"end":{"value":474,"modifier":"EXACT"}},"description":"Abrogates kinase activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"alternativeSequence":{"originalSequence":"DN","alternativeSequences":["GG"]}},{"type":"Mutagenesis","location":{"start":{"value":544,"modifier":"EXACT"},"end":{"value":544,"modifier":"EXACT"}},"description":"Mislocalization of the protein from Golgi apparatus to endoplasmic reticulum.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["W"]}},{"type":"Sequence conflict","location":{"start":{"value":333,"modifier":"EXACT"},"end":{"value":333,"modifier":"EXACT"}},"description":"in Ref. 4; CAJ18570","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["N"]}}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0091","category":"Biological process","name":"Biomineralization"},{"id":"KW-0106","category":"Ligand","name":"Calcium"},{"id":"KW-1015","category":"PTM","name":"Disulfide bond"},{"id":"KW-0256","category":"Cellular component","name":"Endoplasmic reticulum"},{"id":"KW-0325","category":"PTM","name":"Glycoprotein"},{"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"id":"KW-0418","category":"Molecular function","name":"Kinase"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0964","category":"Cellular component","name":"Secreted"},{"id":"KW-0723","category":"Molecular function","name":"Serine/threonine-protein kinase"},{"id":"KW-0735","category":"Domain","name":"Signal-anchor"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"17369251","citationType":"journal article","authors":["Hao J.","Narayanan K.","Muni T.","Ramachandran A.","George A."],"citationCrossReferences":[{"database":"PubMed","id":"17369251"},{"database":"DOI","id":"10.1074/jbc.m701547200"}],"title":"Dentin matrix protein 4, a novel secretory calcium-binding protein that modulates odontoblast differentiation.","publicationDate":"2007","journal":"J. Biol. Chem.","firstPage":"15357","lastPage":"15365","volume":"282"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","SUBCELLULAR LOCATION","TISSUE SPECIFICITY","DEVELOPMENTAL STAGE"]},{"referenceNumber":2,"citation":{"id":"CI-DMIEIPEJ7KGO7","citationType":"submission","authors":["Okazaki N.","Kikuno R.F.","Ohara R.","Inamoto S.","Seino S.","Nishimura M.","Nagase T.","Ohara O.","Koga H."],"title":"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.","publicationDate":"FEB-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Pancreatic islet","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"FVB/N","type":"STRAIN"},{"value":"Liver","type":"TISSUE"},{"value":"Mammary tumor","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"CI-9BO8P0B6TK7T1","citationType":"submission","authors":["Ebert L.","Muenstermann E.","Schatten R.","Henze S.","Bohn E.","Mollenhauer J.","Wiemann S.","Schick M.","Korn B."],"title":"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201).","publicationDate":"JUL-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 331-579"]},{"referenceNumber":5,"citation":{"id":"21549343","citationType":"journal article","authors":["O'Sullivan J.","Bitu C.C.","Daly S.B.","Urquhart J.E.","Barron M.J.","Bhaskar S.S.","Martelli-Junior H.","dos Santos Neto P.E.","Mansilla M.A.","Murray J.C.","Coletta R.D.","Black G.C.","Dixon M.J."],"citationCrossReferences":[{"database":"PubMed","id":"21549343"},{"database":"DOI","id":"10.1016/j.ajhg.2011.04.005"}],"title":"Whole-exome sequencing identifies FAM20A mutations as a cause of amelogenesis imperfecta and gingival hyperplasia syndrome.","publicationDate":"2011","journal":"Am. J. Hum. Genet.","firstPage":"616","lastPage":"620","volume":"88"},"referencePositions":["TISSUE SPECIFICITY"]},{"referenceNumber":6,"citation":{"id":"22900076","citationType":"journal article","authors":["Ishikawa H.O.","Xu A.","Ogura E.","Manning G.","Irvine K.D."],"citationCrossReferences":[{"database":"PubMed","id":"22900076"},{"database":"DOI","id":"10.1371/journal.pone.0042988"}],"title":"The Raine syndrome protein FAM20C is a Golgi kinase that phosphorylates bio-mineralization proteins.","publicationDate":"2012","journal":"PLoS ONE","firstPage":"E42988","lastPage":"E42988","volume":"7"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","SUBCELLULAR LOCATION","MUTAGENESIS OF ILE-241; GLY-261; GLY-374; LEU-383; ASP-446; ASP-453; 473-ASP-ASN-474 AND ARG-544"]},{"referenceNumber":7,"citation":{"id":"22732358","citationType":"journal article","authors":["Vogel P.","Hansen G.M.","Read R.W.","Vance R.B.","Thiel M.","Liu J.","Wronski T.J.","Smith D.D.","Jeter-Jones S.","Brommage R."],"citationCrossReferences":[{"database":"PubMed","id":"22732358"},{"database":"DOI","id":"10.1177/0300985812453177"}],"title":"Amelogenesis imperfecta and other biomineralization defects in Fam20a and Fam20c null mice.","publicationDate":"2012","journal":"Vet. Pathol.","firstPage":"998","lastPage":"1017","volume":"49"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"]},{"referenceNumber":8,"citation":{"id":"25789606","citationType":"journal article","authors":["Cui J.","Xiao J.","Tagliabracci V.S.","Wen J.","Rahdar M.","Dixon J.E."],"citationCrossReferences":[{"database":"PubMed","id":"25789606"},{"database":"DOI","id":"10.7554/elife.06120"}],"title":"A secretory kinase complex regulates extracellular protein phosphorylation.","publicationDate":"2015","journal":"Elife","firstPage":"0","lastPage":"0","volume":"4"},"referencePositions":["FUNCTION","INTERACTION WITH FAM20A","ACTIVITY REGULATION"]},{"referenceNumber":9,"citation":{"id":"29858230","citationType":"journal article","authors":["Zhang J.","Zhu Q.","Wang X.","Yu J.","Chen X.","Wang J.","Wang X.","Xiao J.","Wang C.C.","Wang L."],"citationCrossReferences":[{"database":"PubMed","id":"29858230"},{"database":"DOI","id":"10.15252/embj.201798699"}],"title":"Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1alpha.","publicationDate":"2018","journal":"EMBO J.","firstPage":"0","lastPage":"0","volume":"37"},"referencePositions":["TISSUE SPECIFICITY"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY778962","properties":[{"key":"ProteinId","value":"AAV91326.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK220286","properties":[{"key":"ProteinId","value":"BAD90211.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC004044","properties":[{"key":"ProteinId","value":"AAH04044.3"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC025814","properties":[{"key":"ProteinId","value":"AAH25814.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC025826","properties":[{"key":"ProteinId","value":"AAH25826.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"CT010363","properties":[{"key":"ProteinId","value":"CAJ18570.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS51681.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_085042.2","properties":[{"key":"NucleotideSequenceId","value":"NM_030565.6"}]},{"database":"AlphaFoldDB","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q5MJS3","properties":[{"key":"Number of interactors","value":"635"}]},{"database":"IntAct","id":"Q5MJS3","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"10090.ENSMUSP00000026972","properties":[{"key":"Description","value":"-"}]},{"database":"GlyCosmos","id":"Q5MJS3","properties":[{"key":"glycosylation","value":"1 site, No reported glycans"}]},{"database":"GlyGen","id":"Q5MJS3","properties":[{"key":"glycosylation","value":"2 sites, 2 N-linked glycans (2 sites)"}]},{"database":"iPTMnet","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000026972","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"271540","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"5456","properties":[{"key":"antibodies","value":"151 antibodies from 25 providers"}]},{"database":"DNASU","id":"80752","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000026972.8","properties":[{"key":"ProteinId","value":"ENSMUSP00000026972.8"},{"key":"GeneId","value":"ENSMUSG00000025854.16"}]},{"database":"GeneID","id":"80752","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:80752","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc009afv.2","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:2136853","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"56975","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:2136853","properties":[{"key":"GeneName","value":"Fam20c"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000025854","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG3829","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00950000182951","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_028926_2_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AAENQDW","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"8583677at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-MMU-381426","properties":[{"key":"PathwayName","value":"Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)"}]},{"database":"Reactome","id":"R-MMU-8957275","properties":[{"key":"PathwayName","value":"Post-translational protein phosphorylation"}]},{"database":"SABIO-RK","id":"Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"80752","properties":[{"key":"hits","value":"2 hits in 78 CRISPR screens"}]},{"database":"PRO","id":"PR:Q5MJS3","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 5"}]},{"database":"RNAct","id":"Q5MJS3","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000025854","properties":[{"key":"ExpressionPatterns","value":"Expressed in molar tooth and 241 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q5MJS3","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"21549343"}]},{"database":"GO","id":"GO:0005783","properties":[{"key":"GoTerm","value":"C:endoplasmic reticulum"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005576","properties":[{"key":"GoTerm","value":"C:extracellular region"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17369251"}]},{"database":"GO","id":"GO:0005794","properties":[{"key":"GoTerm","value":"C:Golgi apparatus"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22900076"}]},{"database":"GO","id":"GO:0000139","properties":[{"key":"GoTerm","value":"C:Golgi membrane"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005509","properties":[{"key":"GoTerm","value":"F:calcium ion binding"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17369251"}]},{"database":"GO","id":"GO:0030145","properties":[{"key":"GoTerm","value":"F:manganese ion binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0002020","properties":[{"key":"GoTerm","value":"F:protease binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0004672","properties":[{"key":"GoTerm","value":"F:protein kinase activity"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0106310","properties":[{"key":"GoTerm","value":"F:protein serine kinase activity"},{"key":"GoEvidenceType","value":"EXP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22900076"}]},{"database":"GO","id":"GO:0004674","properties":[{"key":"GoTerm","value":"F:protein serine/threonine kinase activity"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22900076"}]},{"database":"GO","id":"GO:0031214","properties":[{"key":"GoTerm","value":"P:biomineral tissue development"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0097187","properties":[{"key":"GoTerm","value":"P:dentinogenesis"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22732358"}]},{"database":"GO","id":"GO:0070166","properties":[{"key":"GoTerm","value":"P:enamel mineralization"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22732358"}]},{"database":"GO","id":"GO:0071895","properties":[{"key":"GoTerm","value":"P:odontoblast differentiation"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17369251"}]},{"database":"GO","id":"GO:0036179","properties":[{"key":"GoTerm","value":"P:osteoclast maturation"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22615579"}]},{"database":"GO","id":"GO:0030501","properties":[{"key":"GoTerm","value":"P:positive regulation of bone mineralization"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"17369251"},{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22615579"}]},{"database":"GO","id":"GO:0045669","properties":[{"key":"GoTerm","value":"P:positive regulation of osteoblast differentiation"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22615579"}]},{"database":"GO","id":"GO:0006468","properties":[{"key":"GoTerm","value":"P:protein phosphorylation"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"22900076"}]},{"database":"GO","id":"GO:0040036","properties":[{"key":"GoTerm","value":"P:regulation of fibroblast growth factor receptor signaling pathway"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22615579"}]},{"database":"GO","id":"GO:0051174","properties":[{"key":"GoTerm","value":"P:regulation of phosphorus metabolic process"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22615579"}]},{"database":"GO","id":"GO:0001501","properties":[{"key":"GoTerm","value":"P:skeletal system development"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22615579"},{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22732358"}]},{"database":"CDD","id":"cd10471","properties":[{"key":"EntryName","value":"FAM20C_C"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR024869","properties":[{"key":"EntryName","value":"FAM20"}]},{"database":"InterPro","id":"IPR009581","properties":[{"key":"EntryName","value":"FAM20_C"}]},{"database":"PANTHER","id":"PTHR12450","properties":[{"key":"EntryName","value":"DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12450:SF11","properties":[{"key":"EntryName","value":"EXTRACELLULAR SERINE_THREONINE PROTEIN KINASE FAM20C"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF06702","properties":[{"key":"EntryName","value":"Fam20C"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MKMILVRRFRVLILVVFLLACALHIAVDLLPKLDRRATRSSGEPGCSCAQPAAEAAGPGWAQARSRPGESAGGDAGWPNKHTLRILQDFSSDPASNLTSHSLEKLPSAAEPVDHAPRGQEPRSPPPRDPAHRPLLRDPGPRPRVPPPGPSGDGSLLAKLFEHPLYQGAVPPLTEDDVLFNVNSDIRFNPKAAENPDWPHEGAEGAEFLPTGEAAVNLYPNWLKFHIGINRYELYSRHNPAIDALLRDLGSQKITSVAMKSGGTQLKLIMTFQNYGQALFKPMKQTREQETPPDFFYFSDYERHNAEIAAFHLDRILDFRRVPPVAGRMINMTKEIRDVTRDKKLWRTFFVSPANNICFYGECSYYCSTEHALCGRPDQIEGSLAAFLPDLSLAKRKTWRNPWRRSYHKRKKAEWEVDPDYCEEVKQTPPYDSGHRILDIMDMTVFDFLMGNMDRHHYETFEKFGNETFIIHLDNGRGFGKYSHDELSILAPLHQCCRIRRSTYLRLQLLAKEEHKLSLLMAESLQHDKVAPVLYQLHLEALDRRLRIVLQAVRDCVEKDGLSSVVEDDLATEHRASTER","length":579,"molWeight":65766,"crc64":"9DE5F5496AC117FE","md5":"BA2D3B9739FC5BD96CB1D7347F6C5787"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":2,"COFACTOR":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DEVELOPMENTAL STAGE":1,"PTM":3,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1,"SEQUENCE CAUTION":6},"countByFeatureType":{"Propeptide":1,"Chain":1,"Topological domain":2,"Transmembrane":1,"Region":3,"Compositional bias":3,"Active site":1,"Binding site":8,"Site":1,"Glycosylation":1,"Disulfide bond":6,"Mutagenesis":8,"Sequence conflict":1},"uniParcId":"UPI00001F30AC"}}