{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q5DTW2","secondaryAccessions":["Q80VG7"],"uniProtkbId":"SMBT2_MOUSE","entryAudit":{"firstPublicDate":"2005-06-07","lastAnnotationUpdateDate":"2026-01-28","lastSequenceUpdateDate":"2005-06-07","entryVersion":109,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Scm-like with four MBT domains protein 2"}}},"genes":[{"geneName":{"value":"Sfmbt2"},"synonyms":[{"value":"Kiaa1617"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Transcriptional repressor of HOXB13 gene"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Interacts with YY1. Interacts with methylated histones H3K9me2 and H4K20me2 (By similarity)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Nucleus","id":"SL-0191"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q5DTW2-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q5DTW2-2"],"sequenceIds":["VSP_013858","VSP_013859"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18024232"}],"value":"Expressed in testis and, at much lower levels, in ovary"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18024232"}],"value":"Overall, expression is high in extraembryonic tissues, and low in somatic tissues. Expression starts between 8-cell and blastocyst stages. At 7.5 dpc, highly expressed in the ectoplacental cone and, at much lower levels, in the embryonic and extraembryonic ectoderm. At 14.5 dpc, highly expressed in placenta and, at much lower levels, in the yolk sac. Expression in other tissues, including brain, heart, liver, kidney, gut and genital ridges, is very low. Expressed preferentially from the paternal allele in blastocysts, in various tissues at 7.5 dpc, as well as in yolk sac and placenta at 11.5 and 14.5 dpc. Biallelically expressed in brain and other somatic tissues at 11.5 and 14.5 dpc"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"value":"Imprinted gene expressed from the paternal allele in blastocysts and in embryonic tissues early in development. Imprinting is lost in somatic tissues after 7.5 dpc"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Incomplete sequence"}],"commentType":"MISCELLANEOUS","molecule":"Isoform 2"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAD90456.1","note":"Extended N-terminus.","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":938,"modifier":"EXACT"}},"description":"Scm-like with four MBT domains protein 2","featureId":"PRO_0000071970"},{"type":"Repeat","location":{"start":{"value":43,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"MBT 1"},{"type":"Repeat","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"MBT 2"},{"type":"Repeat","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":371,"modifier":"EXACT"}},"description":"MBT 3"},{"type":"Repeat","location":{"start":{"value":379,"modifier":"EXACT"},"end":{"value":475,"modifier":"EXACT"}},"description":"MBT 4"},{"type":"Domain","location":{"start":{"value":868,"modifier":"EXACT"},"end":{"value":931,"modifier":"EXACT"}},"description":"SAM"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":742,"modifier":"EXACT"},"end":{"value":836,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":30,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":765,"modifier":"EXACT"},"end":{"value":777,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":809,"modifier":"EXACT"},"end":{"value":822,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Alternative sequence","location":{"start":{"value":661,"modifier":"EXACT"},"end":{"value":661,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12466296"}],"featureId":"VSP_013858","alternativeSequence":{"originalSequence":"Y","alternativeSequences":["YNKREKGSSLSPASELKDDSASHATKLQKAKIPQ"]}},{"type":"Alternative sequence","location":{"start":{"value":751,"modifier":"EXACT"},"end":{"value":751,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"12466296"}],"featureId":"VSP_013859","alternativeSequence":{"originalSequence":"E","alternativeSequences":["ESLPE"]}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0677","category":"Domain","name":"Repeat"},{"id":"KW-0678","category":"Molecular function","name":"Repressor"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-C3VEIMGK1G3LQ","citationType":"submission","authors":["Okazaki N.","Kikuno R.F.","Ohara R.","Inamoto S.","Nagase T.","Ohara O.","Koga H."],"title":"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.","publicationDate":"FEB-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"Fetal brain","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"12466296","citationType":"journal article","authors":["Tanaka T.S.","Kunath T.","Kimber W.L.","Jaradat S.A.","Stagg C.A.","Usuda M.","Yokota T.","Niwa H.","Rossant J.","Ko M.S."],"citationCrossReferences":[{"database":"PubMed","id":"12466296"},{"database":"DOI","id":"10.1101/gr.670002"}],"title":"Gene expression profiling of embryo-derived stem cells reveals candidate genes associated with pluripotency and lineage specificity.","publicationDate":"2002","journal":"Genome Res.","firstPage":"1921","lastPage":"1928","volume":"12"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 443-938 (ISOFORM 2)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Ectoplacental cone","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"18024232","citationType":"journal article","authors":["Kuzmin A.","Han Z.","Golding M.C.","Mann M.R.","Latham K.E.","Varmuza S."],"citationCrossReferences":[{"database":"PubMed","id":"18024232"},{"database":"DOI","id":"10.1016/j.modgep.2007.09.005"}],"title":"The PcG gene Sfmbt2 is paternally expressed in extraembryonic tissues.","publicationDate":"2008","journal":"Gene Expr. Patterns","firstPage":"107","lastPage":"116","volume":"8"},"referencePositions":["INTERACTION WITH YY1","TISSUE SPECIFICITY","DEVELOPMENTAL STAGE","IMPRINTING","MISCELLANEOUS"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK220408","properties":[{"key":"ProteinId","value":"BAD90456.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY217748","properties":[{"key":"ProteinId","value":"AAO61788.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS57152.1","properties":[{"key":"Description","value":"-"}],"isoformId":"Q5DTW2-2"},{"database":"AlphaFoldDB","id":"Q5DTW2","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q5DTW2","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q5DTW2","properties":[{"key":"Number of interactors","value":"1529"}]},{"database":"STRING","id":"10090.ENSMUSP00000040575","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q5DTW2","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q5DTW2","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000040575","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"257201","properties":[{"key":"Description","value":"-"}],"isoformId":"Q5DTW2-1"},{"database":"ProteomicsDB","id":"257202","properties":[{"key":"Description","value":"-"}],"isoformId":"Q5DTW2-2"},{"database":"AGR","id":"MGI:2447794","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:2447794","properties":[{"key":"GeneName","value":"Sfmbt2"}]},{"database":"eggNOG","id":"KOG3766","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"InParanoid","id":"Q5DTW2","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q5DTW2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Unplaced"}]},{"database":"RNAct","id":"Q5DTW2","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0016235","properties":[{"key":"GoTerm","value":"C:aggresome"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005829","properties":[{"key":"GoTerm","value":"C:cytosol"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0016604","properties":[{"key":"GoTerm","value":"C:nuclear body"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0016607","properties":[{"key":"GoTerm","value":"C:nuclear speck"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0003682","properties":[{"key":"GoTerm","value":"F:chromatin binding"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0042393","properties":[{"key":"GoTerm","value":"F:histone binding"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0003714","properties":[{"key":"GoTerm","value":"F:transcription corepressor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0045892","properties":[{"key":"GoTerm","value":"P:negative regulation of DNA-templated transcription"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0010629","properties":[{"key":"GoTerm","value":"P:negative regulation of gene expression"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"CDD","id":"cd20112","properties":[{"key":"EntryName","value":"MBT_SFMBT2_rpt1"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd20116","properties":[{"key":"EntryName","value":"MBT_SFMBT2_rpt3"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd20118","properties":[{"key":"EntryName","value":"MBT_SFMBT2_rpt4"},{"key":"MatchStatus","value":"1"}]},{"database":"CDD","id":"cd09581","properties":[{"key":"EntryName","value":"SAM_Scm-like-4MBT1_2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"2.30.30.140:FF:000072","properties":[{"key":"EntryName","value":"Scm like with four mbt domains 2"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.10.150.50:FF:000027","properties":[{"key":"EntryName","value":"scm-like with four MBT domains protein 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.30.30.140","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"4"}]},{"database":"Gene3D","id":"3.90.1150.190","properties":[{"key":"EntryName","value":"SLED domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.150.50","properties":[{"key":"EntryName","value":"Transcription Factor, Ets-1"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR004092","properties":[{"key":"EntryName","value":"Mbt"}]},{"database":"InterPro","id":"IPR047353","properties":[{"key":"EntryName","value":"MBT_SFMBT2_rpt1"}]},{"database":"InterPro","id":"IPR047354","properties":[{"key":"EntryName","value":"MBT_SFMBT2_rpt3"}]},{"database":"InterPro","id":"IPR047355","properties":[{"key":"EntryName","value":"MBT_SFMBT2_rpt4"}]},{"database":"InterPro","id":"IPR050548","properties":[{"key":"EntryName","value":"PcG_chromatin_remod_factors"}]},{"database":"InterPro","id":"IPR001660","properties":[{"key":"EntryName","value":"SAM"}]},{"database":"InterPro","id":"IPR013761","properties":[{"key":"EntryName","value":"SAM/pointed_sf"}]},{"database":"InterPro","id":"IPR037604","properties":[{"key":"EntryName","value":"Scm-like-4MBT1/2_SAM"}]},{"database":"InterPro","id":"IPR021987","properties":[{"key":"EntryName","value":"SLED"}]},{"database":"InterPro","id":"IPR038348","properties":[{"key":"EntryName","value":"SLED_sf"}]},{"database":"PANTHER","id":"PTHR12247","properties":[{"key":"EntryName","value":"POLYCOMB GROUP PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12247:SF62","properties":[{"key":"EntryName","value":"SCM-LIKE WITH FOUR MBT DOMAINS PROTEIN 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02820","properties":[{"key":"EntryName","value":"MBT"},{"key":"MatchStatus","value":"4"}]},{"database":"Pfam","id":"PF12140","properties":[{"key":"EntryName","value":"SLED"},{"key":"MatchStatus","value":"1"}]},{"database":"SMART","id":"SM00561","properties":[{"key":"EntryName","value":"MBT"},{"key":"MatchStatus","value":"4"}]},{"database":"SMART","id":"SM00454","properties":[{"key":"EntryName","value":"SAM"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47769","properties":[{"key":"EntryName","value":"SAM/Pointed domain"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF63748","properties":[{"key":"EntryName","value":"Tudor/PWWP/MBT"},{"key":"MatchStatus","value":"4"}]},{"database":"PROSITE","id":"PS51079","properties":[{"key":"EntryName","value":"MBT"},{"key":"MatchStatus","value":"4"}]}],"sequence":{"value":"MERYLPVSKKRNSSSSLEKITGSANGNGTLYSEEDTNLEENDFSWGDYLEETGTRAVPHVSFRHVEISIRSNFQPGMKLEVANKNNPDTYWVATIITTCGQLLLLRYCGYGEDRRADFWCDVIIADLHPVGWCTQNNKVLRPPDAIKDKYSDWTDFLIRELTGSRTAPANLLEGPLRGKGPIDLITVDSLIELQDSQNPFQYWIVSVTENVGGRLRLRYVGLEHTESYDRWLFYLDYRLRPIGWCQENKYRMDPPSDLYYLKLPFEWKCALEKALVLAAESPLPMEVFKDHADLQSHFFTVGMRLETLHISDPFHIYPASVTKVFNSKFFQVAIDDLRPEADKPTMLCHADSLGILPVQWCLKNGVNLAPPKGYSGQDFNWVDYHKQRQAEEAPHFCFKNAFSRGFSKNMKLEAVNPVNPGEVCVATVVSVKGRLLWLHLEGLETPMPDIIVDMDSMDIFPVGWCEANSYPLTTPYKASSKSKRKTVHFKMEKQLLSPVPIEKIPHELCLLPPQMDSPVGAINAKYCCPQLFVNHRCFSGPFLNKGRISELPQSVGPGMCVLVLKEILTLITNAAYKPGRVLRELQLVEDPEWNSQEEILKAKYGGKTYRAVVKIVRTADQVMNFCRQVCAKLECCPNLLSPVLISETCPENCSVHTKTRYTYYYGKRRRVIQSSLRVSNIETPPKSTRRRKRRKSVYVQKRRKSAIVVPAGVPAGVPAGVPEDIPAGIPEGIPASIPESIPEGIPESLPEAIPESIPKGSAQKTEQEKRETLDTARKKTGYHGPAYQTDTSAAQVPFARPRRAVTLRRNSEALKRPPVERARRVRTVPTTASSNNRVKGPLVRIVKPEDSSQSDEEKLILESNPLEWSVTDVVRFIKLTDCAPLARIFQEQDIDGQALLLLTLPTVQECMELKLGPAIKLCHQIERVKVAFYAQYAS","length":938,"molWeight":105981,"crc64":"7D5C7532DD678C33","md5":"5C002BB2B1542476B4017E76B3FCB549"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"TISSUE SPECIFICITY":1,"DEVELOPMENTAL STAGE":1,"MISCELLANEOUS":2,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Repeat":4,"Domain":1,"Region":2,"Compositional bias":3,"Alternative sequence":2},"uniParcId":"UPI000051AE4F"}}