{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q59J78","secondaryAccessions":["Q14A56","Q80VX9"],"uniProtkbId":"NDUF2_MOUSE","entryAudit":{"firstPublicDate":"2005-06-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-04-26","entryVersion":126,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N183"}],"value":"Mimitin"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N183"}],"value":"Myc-induced mitochondrial protein"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N183"}],"value":"MMTN"}]},{"fullName":{"value":"NDUFA12-like protein"}}],"flag":"Precursor"},"genes":[{"geneName":{"value":"Ndufaf2"},"synonyms":[{"value":"Ndufa12l"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N183"}],"value":"Acts as a molecular chaperone for mitochondrial complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Is involved in the initial steps of cilia formation, including removal of CP110 from the mother centrioles, docking of membrane vesicles to the mother centrioles, and establishment of the transition zone"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N183"}],"value":"Interacts with ARMC9"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N183"}],"value":"Mitochondrion","id":"SL-0173"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the complex I NDUFA12 subunit family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"AAP33449.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Transit peptide","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":null,"modifier":"UNKNOWN"}},"description":"Mitochondrion","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Chain","location":{"start":{"value":null,"modifier":"UNKNOWN"},"end":{"value":168,"modifier":"EXACT"}},"description":"NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2","featureId":"PRO_0000020055"},{"type":"Region","location":{"start":{"value":112,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8N183"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0143","category":"Molecular function","name":"Chaperone"},{"id":"KW-0970","category":"Biological process","name":"Cilium biogenesis/degradation"},{"id":"KW-0496","category":"Cellular component","name":"Mitochondrion"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0809","category":"Domain","name":"Transit peptide"}],"references":[{"referenceNumber":1,"citation":{"id":"15774466","citationType":"journal article","authors":["Tsuneoka M.","Teye K.","Arima N.","Soejima M.","Otera H.","Ohashi K.","Koga Y.","Fujita H.","Shirouzu K.","Kimura H.","Koda Y."],"citationCrossReferences":[{"database":"PubMed","id":"15774466"},{"database":"DOI","id":"10.1074/jbc.m501231200"}],"title":"A novel Myc-target gene, mimitin, that is involved in cell proliferation of esophageal squamous cell carcinoma.","publicationDate":"2005","journal":"J. Biol. Chem.","firstPage":"19977","lastPage":"19985","volume":"280"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"]},{"referenceNumber":2,"citation":{"id":"CI-159DJB3TG1GNH","citationType":"submission","authors":["Li X.","Jin Y.","Yue W.","Yan W.","Liu Y."],"title":"A candidate gene related to development of cleft palate.","publicationDate":"MAR-2003","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Brain","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Brain","type":"TISSUE"},{"value":"Brown adipose tissue","type":"TISSUE"},{"value":"Heart","type":"TISSUE"},{"value":"Kidney","type":"TISSUE"},{"value":"Liver","type":"TISSUE"},{"value":"Lung","type":"TISSUE"},{"value":"Pancreas","type":"TISSUE"},{"value":"Spleen","type":"TISSUE"},{"value":"Testis","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB183434","properties":[{"key":"ProteinId","value":"BAD91206.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AY262012","properties":[{"key":"ProteinId","value":"AAP33449.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC116975","properties":[{"key":"ProteinId","value":"AAI16976.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC116977","properties":[{"key":"ProteinId","value":"AAI16978.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS49357.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_001120818.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001127346.2"}]},{"database":"RefSeq","id":"XP_036014098.1","properties":[{"key":"NucleotideSequenceId","value":"XM_036158205.1"}]},{"database":"PDB","id":"8CA5","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"3.90 A"},{"key":"Chains","value":"t=1-168"}]},{"database":"PDBsum","id":"8CA5","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-16518","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"217603","properties":[{"key":"Interactions","value":"3"}]},{"database":"FunCoup","id":"Q59J78","properties":[{"key":"Number of interactors","value":"1580"}]},{"database":"STRING","id":"10090.ENSMUSP00000130532","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q59J78","properties":[{"key":"glycosylation","value":"3 sites, 1 O-linked glycan (3 sites)"}]},{"database":"iPTMnet","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000130532","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"293644","properties":[{"key":"Description","value":"-"}]},{"database":"Pumba","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000163558.3","properties":[{"key":"ProteinId","value":"ENSMUSP00000130532.2"},{"key":"GeneId","value":"ENSMUSG00000068184.8"}]},{"database":"GeneID","id":"75597","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:75597","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc007ruu.2","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:1922847","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"91942","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:1922847","properties":[{"key":"GeneName","value":"Ndufaf2"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000068184","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502S21I","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000002743","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_138027_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"HKTWAGQ","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10255576at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"Reactome","id":"R-MMU-6799198","properties":[{"key":"PathwayName","value":"Complex I biogenesis"}]},{"database":"BioGRID-ORCS","id":"75597","properties":[{"key":"hits","value":"6 hits in 77 CRISPR screens"}]},{"database":"PRO","id":"PR:Q59J78","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 13"}]},{"database":"RNAct","id":"Q59J78","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000068184","properties":[{"key":"ExpressionPatterns","value":"Expressed in manus and 224 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q59J78","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0045271","properties":[{"key":"GoTerm","value":"C:respiratory chain complex I"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0044877","properties":[{"key":"GoTerm","value":"F:protein-containing complex binding"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0060271","properties":[{"key":"GoTerm","value":"P:cilium assembly"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0032981","properties":[{"key":"GoTerm","value":"P:mitochondrial respiratory chain complex I assembly"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"23702311"}]},{"database":"GO","id":"GO:0061179","properties":[{"key":"GoTerm","value":"P:negative regulation of insulin secretion involved in cellular response to glucose stimulus"},{"key":"GoEvidenceType","value":"IMP:MGI"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"22587331"}]},{"database":"InterPro","id":"IPR052618","properties":[{"key":"EntryName","value":"ComplexI_NDUFA12"}]},{"database":"InterPro","id":"IPR007763","properties":[{"key":"EntryName","value":"NDUFA12"}]},{"database":"PANTHER","id":"PTHR32470","properties":[{"key":"EntryName","value":"ADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32470:SF2","properties":[{"key":"EntryName","value":"NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX ASSEMBLY FACTOR 2"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF05071","properties":[{"key":"EntryName","value":"NDUFA12"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSWWSGVWRSVWSALSREVREHVGTDHLGNKYYYVAEYKNWRGQTIREKRIVEAANRKEVDYEAGDIPTEWEAWIRRTRKTPPTMEEILKNEKYREEIKIKSQDFYEKDKLGKETSEELLPSPTATQVKGHASAPYFGREEPSVAPTSTGKTFQPGSWTPEDGKRQSQ","length":168,"molWeight":19628,"crc64":"DB45BAF7DF4D7AD7","md5":"295DA47446A086C602F08D8EAE2B88FD"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Transit peptide":1,"Chain":1,"Region":1,"Compositional bias":1,"Modified residue":1},"uniParcId":"UPI0000021CB3"}}