{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q59646","uniProtkbId":"NORC_PSEAE","entryAudit":{"firstPublicDate":"1998-12-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-01-23","entryVersion":160,"sequenceVersion":3},"annotationScore":3.0,"organism":{"scientificName":"Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)","taxonId":208964,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Pseudomonadales","Pseudomonadaceae","Pseudomonas"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Nitric oxide reductase subunit C"}},"alternativeNames":[{"fullName":{"value":"NOR small subunit"}},{"fullName":{"value":"Nitric oxide reductase cytochrome c subunit"}}]},"genes":[{"geneName":{"value":"norC"},"orderedLocusNames":[{"value":"PA0523"}]}],"comments":[{"texts":[{"value":"Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification)"}],"commentType":"FUNCTION"},{"texts":[{"value":"Heterodimer of cytochromes b (large subunit) and c (small subunit)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"May be attached to the membrane by a signal-anchor"}]},"subcellularLocations":[{"location":{"value":"Cell membrane","id":"SL-0039"},"topology":{"value":"Single-pass membrane protein","id":"SL-9904"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"By nitric oxide"}],"commentType":"INDUCTION"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"Nitric oxide reductase subunit C","featureId":"PRO_0000108424"},{"type":"Transmembrane","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":29,"modifier":"EXACT"}},"description":"Helical; Signal-anchor","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Binding site","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":61,"modifier":"EXACT"}},"description":"covalent","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61717"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00433"}],"ligand":{"name":"heme c","id":"ChEBI:CHEBI:61717"}},{"type":"Binding site","location":{"start":{"value":64,"modifier":"EXACT"},"end":{"value":64,"modifier":"EXACT"}},"description":"covalent","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61717"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00433"}],"ligand":{"name":"heme c","id":"ChEBI:CHEBI:61717"}},{"type":"Binding site","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":65,"modifier":"EXACT"}},"description":"axial binding residue","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:61717"},{"database":"ChEBI","id":"CHEBI:18248"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00433"}],"ligand":{"name":"heme c","id":"ChEBI:CHEBI:61717"},"ligandPart":{"name":"Fe","id":"ChEBI:CHEBI:18248"}},{"type":"Helix","location":{"start":{"value":7,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Helix","location":{"start":{"value":42,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Helix","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":58,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Helix","location":{"start":{"value":61,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Beta strand","location":{"start":{"value":65,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3O0R"}]},{"type":"Beta strand","location":{"start":{"value":73,"modifier":"EXACT"},"end":{"value":75,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Helix","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Turn","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Helix","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":100,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Helix","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFC"}]},{"type":"Helix","location":{"start":{"value":119,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]},{"type":"Beta strand","location":{"start":{"value":141,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"3WFD"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0249","category":"Biological process","name":"Electron transport"},{"id":"KW-0349","category":"Ligand","name":"Heme"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0679","category":"Biological process","name":"Respiratory chain"},{"id":"KW-0735","category":"Domain","name":"Signal-anchor"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0813","category":"Biological process","name":"Transport"}],"references":[{"referenceNumber":1,"citation":{"id":"7711073","citationType":"journal article","authors":["Arai H.","Igarashi Y.","Kodama T."],"citationCrossReferences":[{"database":"PubMed","id":"7711073"},{"database":"DOI","id":"10.1016/0167-4781(95)00018-c"}],"title":"The structural genes for nitric oxide reductase from Pseudomonas aeruginosa.","publicationDate":"1995","journal":"Biochim. Biophys. Acta","firstPage":"279","lastPage":"284","volume":"1261"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"10984043","citationType":"journal article","authors":["Stover C.K.","Pham X.-Q.T.","Erwin A.L.","Mizoguchi S.D.","Warrener P.","Hickey M.J.","Brinkman F.S.L.","Hufnagle W.O.","Kowalik D.J.","Lagrou M.","Garber R.L.","Goltry L.","Tolentino E.","Westbrock-Wadman S.","Yuan Y.","Brody L.L.","Coulter S.N.","Folger K.R.","Kas A.","Larbig K.","Lim R.M.","Smith K.A.","Spencer D.H.","Wong G.K.-S.","Wu Z.","Paulsen I.T.","Reizer J.","Saier M.H. Jr.","Hancock R.E.W.","Lory S.","Olson M.V."],"citationCrossReferences":[{"database":"PubMed","id":"10984043"},{"database":"DOI","id":"10.1038/35023079"}],"title":"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.","publicationDate":"2000","journal":"Nature","firstPage":"959","lastPage":"964","volume":"406"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"D38133","properties":[{"key":"ProteinId","value":"BAA07329.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AE004091","properties":[{"key":"ProteinId","value":"AAG03912.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S53712","properties":[{"key":"EntryName","value":"S53712"}]},{"database":"RefSeq","id":"NP_249214.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002516.2"}]},{"database":"RefSeq","id":"WP_003113238.1","properties":[{"key":"NucleotideSequenceId","value":"NC_002516.2"}]},{"database":"PDB","id":"3O0R","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"C=1-146"}]},{"database":"PDB","id":"3WFB","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.70 A"},{"key":"Chains","value":"C=1-146"}]},{"database":"PDB","id":"3WFC","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.50 A"},{"key":"Chains","value":"C=1-146"}]},{"database":"PDB","id":"3WFD","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.30 A"},{"key":"Chains","value":"C=1-146"}]},{"database":"PDB","id":"3WFE","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.49 A"},{"key":"Chains","value":"C=1-146"}]},{"database":"PDB","id":"5GUW","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"3.20 A"},{"key":"Chains","value":"A/C=1-146"}]},{"database":"PDB","id":"5GUX","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"3.30 A"},{"key":"Chains","value":"C=1-146"}]},{"database":"PDBsum","id":"3O0R","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3WFB","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3WFC","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3WFD","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"3WFE","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5GUW","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"5GUX","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q59646","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q59646","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"208964.PA0523","properties":[{"key":"Description","value":"-"}]},{"database":"TCDB","id":"3.D.4.10.3","properties":[{"key":"FamilyName","value":"the proton-translocating cytochrome oxidase (cox) superfamily"}]},{"database":"PaxDb","id":"208964-PA0523","properties":[{"key":"Description","value":"-"}]},{"database":"ABCD","id":"Q59646","properties":[{"key":"antibodies","value":"1 sequenced antibody"}]},{"database":"DNASU","id":"882200","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"882200","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"pae:PA0523","properties":[{"key":"Description","value":"-"}]},{"database":"PATRIC","id":"fig|208964.12.peg.553","properties":[{"key":"GeneDesignation","value":"-"}]},{"database":"PseudoCAP","id":"PA0523","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_135564_0_0_6","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q59646","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9809720at2","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q59646","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"PAER208964:G1FZ6-528-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"1.7.2.5","properties":[{"key":"OrganismId","value":"5087"}]},{"database":"EvolutionaryTrace","id":"Q59646","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002438","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"AbasyAtlas","id":"Q59646","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0009055","properties":[{"key":"GoTerm","value":"F:electron transfer activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0020037","properties":[{"key":"GoTerm","value":"F:heme binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"Gene3D","id":"1.10.760.10","properties":[{"key":"EntryName","value":"Cytochrome c-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR009056","properties":[{"key":"EntryName","value":"Cyt_c-like_dom"}]},{"database":"InterPro","id":"IPR036909","properties":[{"key":"EntryName","value":"Cyt_c-like_dom_sf"}]},{"database":"InterPro","id":"IPR051811","properties":[{"key":"EntryName","value":"Cytochrome_c550/c551-like"}]},{"database":"PANTHER","id":"PTHR37823","properties":[{"key":"EntryName","value":"CYTOCHROME C-553-LIKE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR37823:SF1","properties":[{"key":"EntryName","value":"CYTOCHROME C-553-LIKE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00034","properties":[{"key":"EntryName","value":"Cytochrom_C"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF46626","properties":[{"key":"EntryName","value":"Cytochrome c"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51007","properties":[{"key":"EntryName","value":"CYTC"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MSETFTKGMARNIYFGGSVFFILLFLALTYHTEKTLPERTNEAAMSAAVVRGKLVWEQNNCVGCHTLLGEGAYFAPELGNVVGRRGGEEGFNTFLQAWMNIQPLNVPGRRAMPQFHLSEGQVDDLAEFLKWSSKIDTNQWPPNKEG","length":146,"molWeight":16344,"crc64":"1C0DB7E59431CC98","md5":"C27421396B85C7DDA1581CEE1AC83C98"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"INDUCTION":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Transmembrane":1,"Binding site":3,"Helix":8,"Beta strand":3,"Turn":1},"uniParcId":"UPI0000165EC1"}}