{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q58905","uniProtkbId":"TYW3_METJA","entryAudit":{"firstPublicDate":"2000-12-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1996-11-01","entryVersion":106,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","commonName":"Methanococcus jannaschii","taxonId":243232,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Methanomada group","Methanococci","Methanococcales","Methanocaldococcaceae","Methanocaldococcus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00266"}],"value":"tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00266"}],"value":"2.1.1.282"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00266"}],"value":"tRNA wyosine derivatives biosynthesis protein Taw3"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00266"}],"value":"taw3"},"orderedLocusNames":[{"value":"MJ1510"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00266"}],"value":"S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:36635"},{"database":"Rhea","id":"RHEA-COMP:10378"},{"database":"Rhea","id":"RHEA-COMP:10379"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:57856"},{"database":"ChEBI","id":"CHEBI:59789"},{"database":"ChEBI","id":"CHEBI:73543"},{"database":"ChEBI","id":"CHEBI:73550"}],"ecNumber":"2.1.1.282","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00266"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00266"}],"value":"Belongs to the TYW3 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase","featureId":"PRO_0000157093"}],"keywords":[{"id":"KW-0489","category":"Molecular function","name":"Methyltransferase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0949","category":"Ligand","name":"S-adenosyl-L-methionine"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0819","category":"Biological process","name":"tRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"8688087","citationType":"journal article","authors":["Bult C.J.","White O.","Olsen G.J.","Zhou L.","Fleischmann R.D.","Sutton G.G.","Blake J.A.","FitzGerald L.M.","Clayton R.A.","Gocayne J.D.","Kerlavage A.R.","Dougherty B.A.","Tomb J.-F.","Adams M.D.","Reich C.I.","Overbeek R.","Kirkness E.F.","Weinstock K.G.","Merrick J.M.","Glodek A.","Scott J.L.","Geoghagen N.S.M.","Weidman J.F.","Fuhrmann J.L.","Nguyen D.","Utterback T.R.","Kelley J.M.","Peterson J.D.","Sadow P.W.","Hanna M.C.","Cotton M.D.","Roberts K.M.","Hurst M.A.","Kaine B.P.","Borodovsky M.","Klenk H.-P.","Fraser C.M.","Smith H.O.","Woese C.R.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"8688087"},{"database":"DOI","id":"10.1126/science.273.5278.1058"}],"title":"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.","publicationDate":"1996","journal":"Science","firstPage":"1058","lastPage":"1073","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"20382657","citationType":"journal article","authors":["de Crecy-Lagard V.","Brochier-Armanet C.","Urbonavicius J.","Fernandez B.","Phillips G.","Lyons B.","Noma A.","Alvarez S.","Droogmans L.","Armengaud J.","Grosjean H."],"citationCrossReferences":[{"database":"PubMed","id":"20382657"},{"database":"DOI","id":"10.1093/molbev/msq096"}],"title":"Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea.","publicationDate":"2010","journal":"Mol. Biol. Evol.","firstPage":"2062","lastPage":"2077","volume":"27"},"referencePositions":["GENE NAME"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L77117","properties":[{"key":"ProteinId","value":"AAB99529.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"E64488","properties":[{"key":"EntryName","value":"E64488"}]},{"database":"RefSeq","id":"WP_010871033.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000909.1"}]},{"database":"AlphaFoldDB","id":"Q58905","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q58905","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q58905","properties":[{"key":"Number of interactors","value":"84"}]},{"database":"STRING","id":"243232.MJ_1510","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"243232-MJ_1510","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAB99529","properties":[{"key":"ProteinId","value":"AAB99529"},{"key":"GeneId","value":"MJ_1510"}]},{"database":"GeneID","id":"1452417","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mja:MJ_1510","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"arCOG04156","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_047426_2_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q58905","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"19299at2157","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q58905","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000805","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0008175","properties":[{"key":"GoTerm","value":"F:tRNA methyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0030488","properties":[{"key":"GoTerm","value":"P:tRNA methylation"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0031591","properties":[{"key":"GoTerm","value":"P:wybutosine biosynthetic process"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"FunFam","id":"3.30.1960.10:FF:000010","properties":[{"key":"EntryName","value":"tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.1960.10","properties":[{"key":"EntryName","value":"tRNA wybutosine-synthesizing-like"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00266","properties":[{"key":"EntryName","value":"TYW3_archaea"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR022908","properties":[{"key":"EntryName","value":"Taw3"}]},{"database":"InterPro","id":"IPR003827","properties":[{"key":"EntryName","value":"tRNA_yW-synthesising"}]},{"database":"InterPro","id":"IPR036602","properties":[{"key":"EntryName","value":"tRNA_yW-synthesising-like_sf"}]},{"database":"NCBIfam","id":"NF003265","properties":[{"key":"EntryName","value":"PRK04235.1-4"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF047731","properties":[{"key":"EntryName","value":"tRNAMtaseTaw3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR48418","properties":[{"key":"EntryName","value":"TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR48418:SF1","properties":[{"key":"EntryName","value":"TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF02676","properties":[{"key":"EntryName","value":"TYW3"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF111278","properties":[{"key":"EntryName","value":"SSo0622-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGFLEDKKRTLMNLELAIREGLVDEEIIPILNKINEIDNYYTTSSCIGRVGIMEIPKDKNPKLYSRWLGKWHHYASYDELFNALKNKKEGYIVFVMNSPILHIACKDIESAKKMLELAIHSGLKASSIKSISDKRVIVEILTTYKVDTPIGEDGEIFVDNNYLKFLLDYSNSKLKRAREILMRWANRLDELKK","length":193,"molWeight":22401,"crc64":"D8E149EC7B4A51C9","md5":"5F76A4920364C20785E2E7E258B30C66"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI000013ACF2"}}