{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q58653","uniProtkbId":"COFD_METJA","entryAudit":{"firstPublicDate":"2004-01-16","lastAnnotationUpdateDate":"2025-06-18","lastSequenceUpdateDate":"1996-11-01","entryVersion":120,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","commonName":"Methanococcus jannaschii","taxonId":243232,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Methanomada group","Methanococci","Methanococcales","Methanocaldococcaceae","Methanocaldococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01257"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11888293"}],"value":"2-phospho-L-lactate transferase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01257"}],"value":"2.7.8.28"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11888293"}],"value":"LPPG:FO 2-phospho-L-lactate transferase"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"11888293"}],"value":"cofD"},"orderedLocusNames":[{"value":"MJ1256"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01257"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}],"value":"Catalyzes the transfer of the 2-phospholactate moiety from (2S)-lactyl-2-diphospho-5'-guanosine to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of oxidized coenzyme F420-0 and GMP"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = oxidized coenzyme F420-0 + GMP + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:63444"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:58115"},{"database":"ChEBI","id":"CHEBI:59435"},{"database":"ChEBI","id":"CHEBI:59904"},{"database":"ChEBI","id":"CHEBI:59907"}],"ecNumber":"2.7.8.28","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01257"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}],"value":"Inhibited by EDTA in vitro"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":1.4,"unit":"umol/min/mg","enzyme":"enzyme with LPPG as substrate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}]},{"velocity":0.1,"unit":"umol/min/mg","enzyme":"enzyme with LPPA as substrate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}]}],"michaelisConstants":[{"constant":32.0,"unit":"uM","substrate":"FO","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}]},{"constant":17.0,"unit":"uM","substrate":"LPPG","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}]},{"constant":515.0,"unit":"uM","substrate":"LPPA","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}]}]},"temperatureDependence":{"texts":[{"value":"Optimum temperature is about 37 degrees Celsius. Thermostable. Still fully active after heating at 80 degrees Celsius for 24 hours. Activity begins to decrease after heating at 98 degrees Celsius for 30 minutes. Inactive after heating at 110 degrees Celsius for 30 minutes."}]}},{"texts":[{"value":"Cofactor biosynthesis; coenzyme F420 biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CofD family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":311,"modifier":"EXACT"}},"description":"2-phospho-L-lactate transferase","featureId":"PRO_0000145772"},{"type":"Binding site","location":{"start":{"value":52,"modifier":"EXACT"},"end":{"value":52,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59904"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01257"}],"ligand":{"name":"7,8-didemethyl-8-hydroxy-5-deazariboflavin","id":"ChEBI:CHEBI:59904"}},{"type":"Binding site","location":{"start":{"value":91,"modifier":"EXACT"},"end":{"value":91,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:59904"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01257"}],"ligand":{"name":"7,8-didemethyl-8-hydroxy-5-deazariboflavin","id":"ChEBI:CHEBI:59904"}},{"type":"Mutagenesis","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"No change in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"11888293"}],"alternativeSequence":{"originalSequence":"S","alternativeSequences":["A"]}}],"keywords":[{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"}],"references":[{"referenceNumber":1,"citation":{"id":"8688087","citationType":"journal article","authors":["Bult C.J.","White O.","Olsen G.J.","Zhou L.","Fleischmann R.D.","Sutton G.G.","Blake J.A.","FitzGerald L.M.","Clayton R.A.","Gocayne J.D.","Kerlavage A.R.","Dougherty B.A.","Tomb J.-F.","Adams M.D.","Reich C.I.","Overbeek R.","Kirkness E.F.","Weinstock K.G.","Merrick J.M.","Glodek A.","Scott J.L.","Geoghagen N.S.M.","Weidman J.F.","Fuhrmann J.L.","Nguyen D.","Utterback T.R.","Kelley J.M.","Peterson J.D.","Sadow P.W.","Hanna M.C.","Cotton M.D.","Roberts K.M.","Hurst M.A.","Kaine B.P.","Borodovsky M.","Klenk H.-P.","Fraser C.M.","Smith H.O.","Woese C.R.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"8688087"},{"database":"DOI","id":"10.1126/science.273.5278.1058"}],"title":"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.","publicationDate":"1996","journal":"Science","firstPage":"1058","lastPage":"1073","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"11888293","citationType":"journal article","authors":["Graupner M.","Xu H.","White R.H."],"citationCrossReferences":[{"database":"PubMed","id":"11888293"},{"database":"DOI","id":"10.1021/bi011937v"}],"title":"Characterization of the 2-phospho-L-lactate transferase enzyme involved in coenzyme F(420) biosynthesis in Methanococcus jannaschii.","publicationDate":"2002","journal":"Biochemistry","firstPage":"3754","lastPage":"3761","volume":"41"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","COFACTOR","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT","MUTAGENESIS OF SER-211"],"referenceComments":[{"value":"ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L77117","properties":[{"key":"ProteinId","value":"AAB99260.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"G64456","properties":[{"key":"EntryName","value":"G64456"}]},{"database":"AlphaFoldDB","id":"Q58653","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q58653","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q58653","properties":[{"key":"Number of interactors","value":"110"}]},{"database":"STRING","id":"243232.MJ_1256","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"243232-MJ_1256","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAB99260","properties":[{"key":"ProteinId","value":"AAB99260"},{"key":"GeneId","value":"MJ_1256"}]},{"database":"KEGG","id":"mja:MJ_1256","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"arCOG04395","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_055795_1_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q58653","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"59563at2157","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q58653","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-12181","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"2.7.8.28","properties":[{"key":"OrganismId","value":"3260"}]},{"database":"SABIO-RK","id":"Q58653","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00071","properties":[{"key":"RectionId","value":"-"}]},{"database":"Proteomes","id":"UP000000805","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0043743","properties":[{"key":"GoTerm","value":"F:LPPG:FO 2-phospho-L-lactate transferase activity"},{"key":"GoEvidenceType","value":"IDA:MENGO"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"11888293"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0052645","properties":[{"key":"GoTerm","value":"P:F420-0 metabolic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd07186","properties":[{"key":"EntryName","value":"CofD_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.8.240","properties":[{"key":"EntryName","value":"CofD-like domain"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.50.10680","properties":[{"key":"EntryName","value":"CofD-like domains"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01257","properties":[{"key":"EntryName","value":"CofD"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002882","properties":[{"key":"EntryName","value":"CofD"}]},{"database":"InterPro","id":"IPR038136","properties":[{"key":"EntryName","value":"CofD-like_dom_sf"}]},{"database":"InterPro","id":"IPR010115","properties":[{"key":"EntryName","value":"FbiA/CofD"}]},{"database":"NCBIfam","id":"TIGR01819","properties":[{"key":"EntryName","value":"F420_cofD"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43007","properties":[{"key":"EntryName","value":"2-PHOSPHO-L-LACTATE TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43007:SF1","properties":[{"key":"EntryName","value":"2-PHOSPHO-L-LACTATE TRANSFERASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01933","properties":[{"key":"EntryName","value":"CofD"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF142338","properties":[{"key":"EntryName","value":"CofD-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MIFVITVLSGGTGTPKLLQGLKRVVNNEELAVIVNTGEDTWIGDLYLSPDVDTVLYTLADLINEETWYGVKEDTFYTHEQLKNLGFDEVLRIGDKDRALKMHKTYYLKRGHKLSEVVDMEKVALGIKAKVIPMTDDRVETKILAKVDGKVDLLKFHDFWVKRKGDVEVLDVIYENSLYAKPCEKAVEAIKNSDLVIIGPSNPITSIGPILSLNGIKELLKDKKVVVVSPIVGNSAVSGPAGKLMKAKGYDVSVKGIYEFYKDIVDVLVIDNVDKEIAKEIPCEVLITNTIMKTLDDKVRLAKNIIEFCGSL","length":311,"molWeight":34599,"crc64":"A2830E42C824F64F","md5":"89AD7D3E86B2587A22C1E9E4944DF508"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"ACTIVITY REGULATION":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":2,"Mutagenesis":1},"uniParcId":"UPI0000064FEB"}}