{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q58499","uniProtkbId":"MFNB_METJA","entryAudit":{"firstPublicDate":"1998-07-15","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1996-11-01","entryVersion":120,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","commonName":"Methanococcus jannaschii","taxonId":243232,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Methanomada group","Methanococci","Methanococcales","Methanocaldococcaceae","Methanocaldococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000305"}],"value":"(5-formylfuran-3-yl)methyl phosphate synthase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"value":"4.2.3.153"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"25905665"}],"value":"4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24977328"}],"value":"4-HFC-P synthase"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000303","source":"PubMed","id":"24977328"}],"value":"mfnB"},"orderedLocusNames":[{"value":"MJ1099"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24977328"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"value":"Catalyzes the formation of 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) from two molecules of glyceraldehyde-3-P (GA-3-P)"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + phosphate + 2 H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:43536"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:43474"},{"database":"ChEBI","id":"CHEBI:59776"},{"database":"ChEBI","id":"CHEBI:83407"}],"ecNumber":"4.2.3.153","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"value":"kcat is 0.026 sec(-1)."}]}},"phDependence":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"value":"Optimum pH is 7.0."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24977328"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"value":"Cofactor biosynthesis; methanofuran biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25372812"}],"value":"Homohexamer. Trimer of dimers"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"}],"value":"Belongs to the MfnB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":235,"modifier":"EXACT"}},"description":"(5-formylfuran-3-yl)methyl phosphate synthase","featureId":"PRO_0000134865"},{"type":"Active site","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Schiff-base intermediate with substrate","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25905665"}]},{"type":"Active site","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00681"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"25905665"}]},{"type":"Mutagenesis","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":25,"modifier":"EXACT"}},"description":"Lack of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":27,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"Lack of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":85,"modifier":"EXACT"},"end":{"value":85,"modifier":"EXACT"}},"description":"Lack of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["R"]}},{"type":"Mutagenesis","location":{"start":{"value":151,"modifier":"EXACT"},"end":{"value":151,"modifier":"EXACT"}},"description":"Lack of activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"alternativeSequence":{"originalSequence":"D","alternativeSequences":["N"]}},{"type":"Mutagenesis","location":{"start":{"value":155,"modifier":"EXACT"},"end":{"value":155,"modifier":"EXACT"}},"description":"Almost no change in activity.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25905665"}],"alternativeSequence":{"originalSequence":"K","alternativeSequences":["R"]}},{"type":"Beta strand","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":5,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":19,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Beta strand","location":{"start":{"value":22,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":29,"modifier":"EXACT"},"end":{"value":31,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":48,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Beta strand","location":{"start":{"value":53,"modifier":"EXACT"},"end":{"value":62,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":66,"modifier":"EXACT"},"end":{"value":78,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Beta strand","location":{"start":{"value":82,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":94,"modifier":"EXACT"},"end":{"value":111,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Beta strand","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":122,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":125,"modifier":"EXACT"},"end":{"value":127,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":130,"modifier":"EXACT"},"end":{"value":132,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":133,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":136,"modifier":"EXACT"},"end":{"value":143,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Beta strand","location":{"start":{"value":146,"modifier":"EXACT"},"end":{"value":151,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":160,"modifier":"EXACT"},"end":{"value":163,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":178,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Beta strand","location":{"start":{"value":182,"modifier":"EXACT"},"end":{"value":185,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":191,"modifier":"EXACT"},"end":{"value":193,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":194,"modifier":"EXACT"},"end":{"value":199,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Beta strand","location":{"start":{"value":203,"modifier":"EXACT"},"end":{"value":208,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Helix","location":{"start":{"value":209,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]},{"type":"Beta strand","location":{"start":{"value":212,"modifier":"EXACT"},"end":{"value":214,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4RC1"}]},{"type":"Turn","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":219,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4RC1"}]},{"type":"Helix","location":{"start":{"value":224,"modifier":"EXACT"},"end":{"value":233,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4U9P"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0704","category":"Ligand","name":"Schiff base"}],"references":[{"referenceNumber":1,"citation":{"id":"8688087","citationType":"journal article","authors":["Bult C.J.","White O.","Olsen G.J.","Zhou L.","Fleischmann R.D.","Sutton G.G.","Blake J.A.","FitzGerald L.M.","Clayton R.A.","Gocayne J.D.","Kerlavage A.R.","Dougherty B.A.","Tomb J.-F.","Adams M.D.","Reich C.I.","Overbeek R.","Kirkness E.F.","Weinstock K.G.","Merrick J.M.","Glodek A.","Scott J.L.","Geoghagen N.S.M.","Weidman J.F.","Fuhrmann J.L.","Nguyen D.","Utterback T.R.","Kelley J.M.","Peterson J.D.","Sadow P.W.","Hanna M.C.","Cotton M.D.","Roberts K.M.","Hurst M.A.","Kaine B.P.","Borodovsky M.","Klenk H.-P.","Fraser C.M.","Smith H.O.","Woese C.R.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"8688087"},{"database":"DOI","id":"10.1126/science.273.5278.1058"}],"title":"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.","publicationDate":"1996","journal":"Science","firstPage":"1058","lastPage":"1073","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"24977328","citationType":"journal article","authors":["Miller D.","Wang Y.","Xu H.","Harich K.","White R.H."],"citationCrossReferences":[{"database":"PubMed","id":"24977328"},{"database":"DOI","id":"10.1021/bi500615p"}],"title":"Biosynthesis of the 5-(aminomethyl)-3-furanmethanol moiety of methanofuran.","publicationDate":"2014","journal":"Biochemistry","firstPage":"4635","lastPage":"4647","volume":"53"},"referencePositions":["FUNCTION","PATHWAY"]},{"referenceNumber":3,"citation":{"id":"25905665","citationType":"journal article","authors":["Wang Y.","Jones M.K.","Xu H.","Ray W.K.","White R.H."],"citationCrossReferences":[{"database":"PubMed","id":"25905665"},{"database":"DOI","id":"10.1021/acs.biochem.5b00176"}],"title":"Mechanism of the enzymatic synthesis of 4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate (4-HFC-P) from glyceraldehyde-3-phosphate catalyzed by 4-HFC-P synthase.","publicationDate":"2015","journal":"Biochemistry","firstPage":"2997","lastPage":"3008","volume":"54"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","BIOPHYSICOCHEMICAL PROPERTIES","REACTION MECHANISM","PATHWAY","ACTIVE SITE","MUTAGENESIS OF ASP-25; LYS-27; LYS-85; ASP-151 AND LYS-155"]},{"referenceNumber":4,"citation":{"id":"25372812","citationType":"journal article","authors":["Bobik T.A.","Morales E.J.","Shin A.","Cascio D.","Sawaya M.R.","Arbing M.","Yeates T.O.","Rasche M.E."],"citationCrossReferences":[{"database":"PubMed","id":"25372812"},{"database":"DOI","id":"10.1107/s2053230x1402130x"}],"title":"Structure of the methanofuran/methanopterin-biosynthetic enzyme MJ1099 from Methanocaldococcus jannaschii.","publicationDate":"2014","journal":"Acta Crystallogr. F","firstPage":"1472","lastPage":"1479","volume":"70"},"referencePositions":["X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS)","SUBUNIT"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L77117","properties":[{"key":"ProteinId","value":"AAB99102.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"B64437","properties":[{"key":"EntryName","value":"B64437"}]},{"database":"RefSeq","id":"WP_010870611.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000909.1"}]},{"database":"PDB","id":"4RC1","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"2.40 A"},{"key":"Chains","value":"A/B/C/D/E/F/G/H/I=1-235"}]},{"database":"PDB","id":"4U9P","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.70 A"},{"key":"Chains","value":"A/B/C=1-235"}]},{"database":"PDBsum","id":"4RC1","properties":[{"key":"Description","value":"-"}]},{"database":"PDBsum","id":"4U9P","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q58499","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q58499","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q58499","properties":[{"key":"Number of interactors","value":"110"}]},{"database":"STRING","id":"243232.MJ_1099","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"243232-MJ_1099","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"1451996","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAB99102","properties":[{"key":"ProteinId","value":"AAB99102"},{"key":"GeneId","value":"MJ_1099"}]},{"database":"GeneID","id":"1451996","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mja:MJ_1099","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"arCOG04482","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_068659_0_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q58499","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"81473at2157","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q58499","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"MetaCyc:MONOMER-18937","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.2.3.153","properties":[{"key":"OrganismId","value":"3260"}]},{"database":"UniPathway","id":"UPA00080","properties":[{"key":"RectionId","value":"-"}]},{"database":"EvolutionaryTrace","id":"Q58499","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000805","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0016830","properties":[{"key":"GoTerm","value":"F:carbon-carbon lyase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:2001120","properties":[{"key":"GoTerm","value":"P:methanofuran biosynthetic process"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00945","properties":[{"key":"EntryName","value":"Aldolase_Class_I"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00681","properties":[{"key":"EntryName","value":"MfnB"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007565","properties":[{"key":"EntryName","value":"4HFCP_synth"}]},{"database":"InterPro","id":"IPR035081","properties":[{"key":"EntryName","value":"4HFCP_synth_arc"}]},{"database":"InterPro","id":"IPR011060","properties":[{"key":"EntryName","value":"RibuloseP-bd_barrel"}]},{"database":"NCBIfam","id":"NF002573","properties":[{"key":"EntryName","value":"PRK02227.1-1"},{"key":"MatchStatus","value":"1"}]},{"database":"NCBIfam","id":"NF002575","properties":[{"key":"EntryName","value":"PRK02227.1-3"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04476","properties":[{"key":"EntryName","value":"4HFCP_synth"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF015957","properties":[{"key":"EntryName","value":"UCP015957"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51569","properties":[{"key":"EntryName","value":"Aldolase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF51366","properties":[{"key":"EntryName","value":"Ribulose-phoshate binding barrel"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MILLVSPIDVEEAKEAIAGGADIIDVKNPKEGSLGANFPWMIKAIREVTPKDLLVSATVGDVPYKPGTISLAAVGAAISGADYIKVGLYGVKNYYQAVELMKNVVRAVKDIDENKIVVAAGYADAYRVGAVEPLIVPKIARDAGCDVAMLDTAIKDGKTLFDFQSKEILAEFVDEAHSYGLKCALAGSIKKEHIPILKEIGTDIVGVRGAACKGGDRNNGRIDRELVKELKELCK","length":235,"molWeight":25099,"crc64":"9F425AC7C6096E85","md5":"D003911ACC320B7FD961A847A7929227"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":2,"Mutagenesis":5,"Beta strand":9,"Helix":15,"Turn":1},"uniParcId":"UPI0000139FE1"}}