{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q57920","uniProtkbId":"HJC_METJA","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1996-11-01","entryVersion":114,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","commonName":"Methanococcus jannaschii","taxonId":243232,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Methanomada group","Methanococci","Methanococcales","Methanocaldococcaceae","Methanocaldococcus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"Crossover junction endodeoxyribonuclease Hjc"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"Hjc"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"3.1.21.10"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"Holliday junction resolvase Hjc"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"hjc"},"orderedLocusNames":[{"value":"MJ0497"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).","ecNumber":"3.1.21.10","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}]}},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"Binds 1 Mg(2+) ion per subunit."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"value":"Belongs to the Holliday junction resolvase Hjc family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":133,"modifier":"EXACT"}},"description":"Crossover junction endodeoxyribonuclease Hjc","featureId":"PRO_0000106899"},{"type":"Active site","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}]},{"type":"Binding site","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":36,"modifier":"EXACT"},"end":{"value":36,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Binding site","location":{"start":{"value":49,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01490"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420"}},{"type":"Site","location":{"start":{"value":51,"modifier":"EXACT"},"end":{"value":51,"modifier":"EXACT"}},"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000255"}]}],"keywords":[{"id":"KW-0227","category":"Biological process","name":"DNA damage"},{"id":"KW-0233","category":"Biological process","name":"DNA recombination"},{"id":"KW-0234","category":"Biological process","name":"DNA repair"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-0255","category":"Molecular function","name":"Endonuclease"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0460","category":"Ligand","name":"Magnesium"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"8688087","citationType":"journal article","authors":["Bult C.J.","White O.","Olsen G.J.","Zhou L.","Fleischmann R.D.","Sutton G.G.","Blake J.A.","FitzGerald L.M.","Clayton R.A.","Gocayne J.D.","Kerlavage A.R.","Dougherty B.A.","Tomb J.-F.","Adams M.D.","Reich C.I.","Overbeek R.","Kirkness E.F.","Weinstock K.G.","Merrick J.M.","Glodek A.","Scott J.L.","Geoghagen N.S.M.","Weidman J.F.","Fuhrmann J.L.","Nguyen D.","Utterback T.R.","Kelley J.M.","Peterson J.D.","Sadow P.W.","Hanna M.C.","Cotton M.D.","Roberts K.M.","Hurst M.A.","Kaine B.P.","Borodovsky M.","Klenk H.-P.","Fraser C.M.","Smith H.O.","Woese C.R.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"8688087"},{"database":"DOI","id":"10.1126/science.273.5278.1058"}],"title":"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.","publicationDate":"1996","journal":"Science","firstPage":"1058","lastPage":"1073","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L77117","properties":[{"key":"ProteinId","value":"AAB98490.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"A64362","properties":[{"key":"EntryName","value":"A64362"}]},{"database":"RefSeq","id":"WP_010869998.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000909.1"}]},{"database":"AlphaFoldDB","id":"Q57920","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q57920","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"243232.MJ_0497","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"243232-MJ_0497","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAB98490","properties":[{"key":"ProteinId","value":"AAB98490"},{"key":"GeneId","value":"MJ_0497"}]},{"database":"GeneID","id":"1451359","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mja:MJ_0497","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"arCOG00919","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_139546_2_0_2","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q57920","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"34330at2157","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q57920","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000805","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0008821","properties":[{"key":"GoTerm","value":"F:crossover junction DNA endonuclease activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0000287","properties":[{"key":"GoTerm","value":"F:magnesium ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0003676","properties":[{"key":"GoTerm","value":"F:nucleic acid binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006310","properties":[{"key":"GoTerm","value":"P:DNA recombination"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0006281","properties":[{"key":"GoTerm","value":"P:DNA repair"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00523","properties":[{"key":"EntryName","value":"Holliday_junction_resolvase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.1350.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_01490","properties":[{"key":"EntryName","value":"HJ_Resolv_Hjc"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002732","properties":[{"key":"EntryName","value":"Hjc"}]},{"database":"InterPro","id":"IPR014428","properties":[{"key":"EntryName","value":"Hjc_arc"}]},{"database":"InterPro","id":"IPR011335","properties":[{"key":"EntryName","value":"Restrct_endonuc-II-like"}]},{"database":"InterPro","id":"IPR011856","properties":[{"key":"EntryName","value":"tRNA_endonuc-like_dom_sf"}]},{"database":"NCBIfam","id":"NF040854","properties":[{"key":"EntryName","value":"Hol_resolv_Hjc"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39651","properties":[{"key":"EntryName","value":"HOLLIDAY JUNCTION RESOLVASE HJC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR39651:SF1","properties":[{"key":"EntryName","value":"HOLLIDAY JUNCTION RESOLVASE HJC"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01870","properties":[{"key":"EntryName","value":"Hjc"},{"key":"MatchStatus","value":"1"}]},{"database":"PIRSF","id":"PIRSF004985","properties":[{"key":"EntryName","value":"Hlld_jn_rslvs_ar"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF52980","properties":[{"key":"EntryName","value":"Restriction endonuclease-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MRHKYRKGSSFERELKRLLEKEGFAVIRSAGSKGVDLIAGRKGEVLIFECKTSSKTKFYINKEDIEKLISFSEIFGGKPYLAIKFNGEMLFINPFLLSTNGKNYVIDERIKAIAIDFYEVIGRGKQLKIDDLI","length":133,"molWeight":15232,"crc64":"E49A8A128AF16506","md5":"4AD5204A3FFDB4044DB15FA4115F3EB9"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBUNIT":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1,"Binding site":3,"Site":1},"uniParcId":"UPI00001398B2"}}