{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q57589","uniProtkbId":"Y125_METJA","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":104,"sequenceVersion":1},"annotationScore":2.0,"organism":{"scientificName":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","commonName":"Methanococcus jannaschii","taxonId":243232,"lineage":["Archaea","Methanobacteriati","Methanobacteriota","Methanomada group","Methanococci","Methanococcales","Methanocaldococcaceae","Methanocaldococcus"]},"proteinExistence":"3: Inferred from homology","proteinDescription":{"recommendedName":{"fullName":{"value":"Putative RNase MJ0125"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8ECH6"}],"value":"3.1.-.-"}]},"alternativeNames":[{"fullName":{"value":"Putative toxin MJ0125"}}]},"genes":[{"orderedLocusNames":[{"value":"MJ0125"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8ECH6"}],"value":"Probable toxic component of a putative type VII toxin-antitoxin (TA) system, probably an RNase. Probably neutralized by cognate antitoxin MJ0126. Neutralization may be due to AMPylation by MJ0126"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8ECH6"}],"value":"Homodimer, probably forms a complex with cognate antitoxin MJ0126"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0B0QJR1"}],"value":"Modified by cognate antitoxin MJ0126; probably at least 2 successive AMPylation events occur on Tyr-83"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the HepT RNase toxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":116,"modifier":"EXACT"}},"description":"Putative RNase MJ0125","featureId":"PRO_0000158259"},{"type":"Motif","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"RX(4)HXY motif","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0B0QJR1"}]},{"type":"Active site","location":{"start":{"value":76,"modifier":"EXACT"},"end":{"value":76,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0B0QJR1"}]},{"type":"Active site","location":{"start":{"value":81,"modifier":"EXACT"},"end":{"value":81,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0B0QJR1"}]},{"type":"Modified residue","location":{"start":{"value":83,"modifier":"EXACT"},"end":{"value":83,"modifier":"EXACT"}},"description":"O-di-AMP-tyrosine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A0A0B0QJR1"}]}],"keywords":[{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0540","category":"Molecular function","name":"Nuclease"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-1277","category":"Biological process","name":"Toxin-antitoxin system"}],"references":[{"referenceNumber":1,"citation":{"id":"8688087","citationType":"journal article","authors":["Bult C.J.","White O.","Olsen G.J.","Zhou L.","Fleischmann R.D.","Sutton G.G.","Blake J.A.","FitzGerald L.M.","Clayton R.A.","Gocayne J.D.","Kerlavage A.R.","Dougherty B.A.","Tomb J.-F.","Adams M.D.","Reich C.I.","Overbeek R.","Kirkness E.F.","Weinstock K.G.","Merrick J.M.","Glodek A.","Scott J.L.","Geoghagen N.S.M.","Weidman J.F.","Fuhrmann J.L.","Nguyen D.","Utterback T.R.","Kelley J.M.","Peterson J.D.","Sadow P.W.","Hanna M.C.","Cotton M.D.","Roberts K.M.","Hurst M.A.","Kaine B.P.","Borodovsky M.","Klenk H.-P.","Fraser C.M.","Smith H.O.","Woese C.R.","Venter J.C."],"citationCrossReferences":[{"database":"PubMed","id":"8688087"},{"database":"DOI","id":"10.1126/science.273.5278.1058"}],"title":"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.","publicationDate":"1996","journal":"Science","firstPage":"1058","lastPage":"1073","volume":"273"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"L77117","properties":[{"key":"ProteinId","value":"AAB98105.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"E64315","properties":[{"key":"EntryName","value":"E64315"}]},{"database":"RefSeq","id":"WP_010869618.1","properties":[{"key":"NucleotideSequenceId","value":"NC_000909.1"}]},{"database":"AlphaFoldDB","id":"Q57589","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q57589","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q57589","properties":[{"key":"Number of interactors","value":"3"}]},{"database":"STRING","id":"243232.MJ_0125","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"243232-MJ_0125","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"AAB98105","properties":[{"key":"ProteinId","value":"AAB98105"},{"key":"GeneId","value":"MJ_0125"}]},{"database":"GeneID","id":"1450967","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mja:MJ_0125","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"arCOG05024","properties":[{"key":"ToxonomicScope","value":"Archaea"}]},{"database":"HOGENOM","id":"CLU_142825_3_3_2","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q57589","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"318716at2157","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q57589","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000805","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0110001","properties":[{"key":"GoTerm","value":"C:toxin-antitoxin complex"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0004540","properties":[{"key":"GoTerm","value":"F:RNA nuclease activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"InterPro","id":"IPR008201","properties":[{"key":"EntryName","value":"HepT-like"}]},{"database":"PANTHER","id":"PTHR34139:SF1","properties":[{"key":"EntryName","value":"RNASE MJ1380-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR34139","properties":[{"key":"EntryName","value":"UPF0331 PROTEIN MJ0127"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01934","properties":[{"key":"EntryName","value":"HepT-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPKRDIKAFLYDILSYMDDIINFTKDMDYEEFINNKAIKYAVIRCLEVIGEAVKKIPKDIREKYPHIPFKELAGMRDKLIHQYFGVDYLTVWETAKYEIPEIKKEFEKIIKDLEEK","length":116,"molWeight":13970,"crc64":"7A77740A495160EF","md5":"81AB109725C3E3A45E6F5FF5234DC4D2"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"PTM":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Motif":1,"Active site":2,"Modified residue":1},"uniParcId":"UPI00001392F5"}}