{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q56WM6","secondaryAccessions":["Q8GWY8","Q9C6V5"],"uniProtkbId":"ROGFE_ARATH","entryAudit":{"firstPublicDate":"2013-10-16","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-05-10","entryVersion":111,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Arabidopsis thaliana","commonName":"Mouse-ear cress","taxonId":3702,"lineage":["Eukaryota","Viridiplantae","Streptophyta","Embryophyta","Tracheophyta","Spermatophyta","Magnoliopsida","eudicotyledons","Gunneridae","Pentapetalae","rosids","malvids","Brassicales","Brassicaceae","Camelineae","Arabidopsis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Rop guanine nucleotide exchange factor 14"},"shortNames":[{"value":"AtRopGEF14"}]},"alternativeNames":[{"fullName":{"value":"Rho of plants guanine nucleotide exchange factor 14"}}]},"genes":[{"geneName":{"value":"ROPGEF14"},"orderedLocusNames":[{"value":"At1g31650"}],"orfNames":[{"value":"F27M3.15"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"Guanine-nucleotide exchange factor (GEF) that acts as an activator of Rop (Rho of plants) GTPases by promoting the exchange of GDP for GTP"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16415208"}],"value":"Interacts with ARAC11/ROP1"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"value":"Localizes to the apical region of the pollen tube plasma membrane"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16415208"}],"value":"Cell membrane","id":"SL-0039"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16415208"}],"value":"Expressed in pollen grains"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250"}],"value":"The PRONE (plant-specific Rop nucleotide exchanger) domain is responsible for the GEF activity"}],"commentType":"DOMAIN"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous gene model prediction","sequence":"AAG60161.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":576,"modifier":"EXACT"}},"description":"Rop guanine nucleotide exchange factor 14","featureId":"PRO_0000423899"},{"type":"Domain","location":{"start":{"value":127,"modifier":"EXACT"},"end":{"value":500,"modifier":"EXACT"}},"description":"PRONE","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00663"}]},{"type":"Region","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":300,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":491,"modifier":"EXACT"},"end":{"value":520,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":273,"modifier":"EXACT"},"end":{"value":283,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":435,"modifier":"EXACT"},"end":{"value":435,"modifier":"EXACT"}},"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19245862"}]},{"type":"Sequence conflict","location":{"start":{"value":260,"modifier":"EXACT"},"end":{"value":260,"modifier":"EXACT"}},"description":"in Ref. 4; BAC43154","evidences":[{"evidenceCode":"ECO:0000305"}],"alternativeSequence":{"originalSequence":"T","alternativeSequences":["R"]}}],"keywords":[{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0344","category":"Molecular function","name":"Guanine-nucleotide releasing factor"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"11130712","citationType":"journal article","authors":["Theologis A.","Ecker J.R.","Palm C.J.","Federspiel N.A.","Kaul S.","White O.","Alonso J.","Altafi H.","Araujo R.","Bowman C.L.","Brooks S.Y.","Buehler E.","Chan A.","Chao Q.","Chen H.","Cheuk R.F.","Chin C.W.","Chung M.K.","Conn L.","Conway A.B.","Conway A.R.","Creasy T.H.","Dewar K.","Dunn P.","Etgu P.","Feldblyum T.V.","Feng J.-D.","Fong B.","Fujii C.Y.","Gill J.E.","Goldsmith A.D.","Haas B.","Hansen N.F.","Hughes B.","Huizar L.","Hunter J.L.","Jenkins J.","Johnson-Hopson C.","Khan S.","Khaykin E.","Kim C.J.","Koo H.L.","Kremenetskaia I.","Kurtz D.B.","Kwan A.","Lam B.","Langin-Hooper S.","Lee A.","Lee J.M.","Lenz C.A.","Li J.H.","Li Y.-P.","Lin X.","Liu S.X.","Liu Z.A.","Luros J.S.","Maiti R.","Marziali A.","Militscher J.","Miranda M.","Nguyen M.","Nierman W.C.","Osborne B.I.","Pai G.","Peterson J.","Pham P.K.","Rizzo M.","Rooney T.","Rowley D.","Sakano H.","Salzberg S.L.","Schwartz J.R.","Shinn P.","Southwick A.M.","Sun H.","Tallon L.J.","Tambunga G.","Toriumi M.J.","Town C.D.","Utterback T.","Van Aken S.","Vaysberg M.","Vysotskaia V.S.","Walker M.","Wu D.","Yu G.","Fraser C.M.","Venter J.C.","Davis R.W."],"citationCrossReferences":[{"database":"PubMed","id":"11130712"},{"database":"DOI","id":"10.1038/35048500"}],"title":"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.","publicationDate":"2000","journal":"Nature","firstPage":"816","lastPage":"820","volume":"408"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"27862469","citationType":"journal article","authors":["Cheng C.Y.","Krishnakumar V.","Chan A.P.","Thibaud-Nissen F.","Schobel S.","Town C.D."],"citationCrossReferences":[{"database":"PubMed","id":"27862469"},{"database":"DOI","id":"10.1111/tpj.13415"}],"title":"Araport11: a complete reannotation of the Arabidopsis thaliana reference genome.","publicationDate":"2017","journal":"Plant J.","firstPage":"789","lastPage":"804","volume":"89"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"CI-CI4AM1G71UC0S","citationType":"submission","authoringGroup":["The Arabidopsis Information Resource (TAIR)"],"title":"Community-consensus reference genome of Arabidopsis thaliana Col-0.","publicationDate":"FEB-2026","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"11910074","citationType":"journal article","authors":["Seki M.","Narusaka M.","Kamiya A.","Ishida J.","Satou M.","Sakurai T.","Nakajima M.","Enju A.","Akiyama K.","Oono Y.","Muramatsu M.","Hayashizaki Y.","Kawai J.","Carninci P.","Itoh M.","Ishii Y.","Arakawa T.","Shibata K.","Shinagawa A.","Shinozaki K."],"citationCrossReferences":[{"database":"PubMed","id":"11910074"},{"database":"DOI","id":"10.1126/science.1071006"}],"title":"Functional annotation of a full-length Arabidopsis cDNA collection.","publicationDate":"2002","journal":"Science","firstPage":"141","lastPage":"145","volume":"296"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":5,"citation":{"id":"CI-B9D7AITG0S7MM","citationType":"submission","authors":["Totoki Y.","Seki M.","Ishida J.","Nakajima M.","Enju A.","Kamiya A.","Narusaka M.","Shin-i T.","Nakagawa M.","Sakamoto N.","Oishi K.","Kohara Y.","Kobayashi M.","Toyoda A.","Sakaki Y.","Sakurai T.","Iida K.","Akiyama K.","Satou M.","Toyoda T.","Konagaya A.","Carninci P.","Kawai J.","Hayashizaki Y.","Shinozaki K."],"title":"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.","publicationDate":"MAR-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]},{"referenceNumber":6,"citation":{"id":"15980860","citationType":"journal article","authors":["Berken A.","Thomas C.","Wittinghofer A."],"citationCrossReferences":[{"database":"PubMed","id":"15980860"},{"database":"DOI","id":"10.1038/nature03883"}],"title":"A new family of RhoGEFs activates the Rop molecular switch in plants.","publicationDate":"2005","journal":"Nature","firstPage":"1176","lastPage":"1180","volume":"436"},"referencePositions":["GENE FAMILY"]},{"referenceNumber":7,"citation":{"id":"16415208","citationType":"journal article","authors":["Gu Y.","Li S.","Lord E.M.","Yang Z."],"citationCrossReferences":[{"database":"PubMed","id":"16415208"},{"database":"DOI","id":"10.1105/tpc.105.036434"}],"title":"Members of a novel class of Arabidopsis Rho guanine nucleotide exchange factors control Rho GTPase-dependent polar growth.","publicationDate":"2006","journal":"Plant Cell","firstPage":"366","lastPage":"381","volume":"18"},"referencePositions":["INTERACTION WITH ARAC11/ROP1","SUBCELLULAR LOCATION","TISSUE SPECIFICITY"]},{"referenceNumber":8,"citation":{"id":"19245862","citationType":"journal article","authors":["Jones A.M.E.","MacLean D.","Studholme D.J.","Serna-Sanz A.","Andreasson E.","Rathjen J.P.","Peck S.C."],"citationCrossReferences":[{"database":"PubMed","id":"19245862"},{"database":"DOI","id":"10.1016/j.jprot.2009.02.004"}],"title":"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana.","publicationDate":"2009","journal":"J. Proteomics","firstPage":"439","lastPage":"451","volume":"72"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-435","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"cv. Columbia","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AC074360","properties":[{"key":"ProteinId","value":"AAG60161.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"CP002684","properties":[{"key":"ProteinId","value":"AEE31380.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"OZ408683","properties":[{"key":"ProteinId","value":"CAO7029343.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"AK118553","properties":[{"key":"ProteinId","value":"BAC43154.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK222010","properties":[{"key":"ProteinId","value":"BAD94650.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001320846.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001332971.1"}]},{"database":"RefSeq","id":"NP_174446.2","properties":[{"key":"NucleotideSequenceId","value":"NM_102900.5"}]},{"database":"AlphaFoldDB","id":"Q56WM6","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q56WM6","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"25287","properties":[{"key":"Interactions","value":"2"}]},{"database":"DIP","id":"DIP-59398N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q56WM6","properties":[{"key":"Number of interactors","value":"1136"}]},{"database":"IntAct","id":"Q56WM6","properties":[{"key":"Interactions","value":"1"}]},{"database":"STRING","id":"3702.Q56WM6","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q56WM6","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"3702-Q56WM6","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"228075","properties":[{"key":"Description","value":"-"}]},{"database":"GeneID","id":"840052","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"ath:AT1G31650","properties":[{"key":"Description","value":"-"}]},{"database":"Araport","id":"AT1G31650","properties":[{"key":"Description","value":"-"}]},{"database":"TAIR","id":"AT1G31650","properties":[{"key":"GeneName","value":"ROPGEF14"}]},{"database":"eggNOG","id":"ENOG502QPJS","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_019073_3_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q56WM6","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q56WM6","properties":[{"key":"Description","value":"-"}]},{"database":"PRO","id":"PR:Q56WM6","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000006548","properties":[{"key":"Component","value":"Chromosome 1"}]},{"database":"ExpressionAtlas","id":"Q56WM6","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005739","properties":[{"key":"GoTerm","value":"C:mitochondrion"},{"key":"GoEvidenceType","value":"ISM:TAIR"}]},{"database":"GO","id":"GO:0005886","properties":[{"key":"GoTerm","value":"C:plasma membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005085","properties":[{"key":"GoTerm","value":"F:guanyl-nucleotide exchange factor activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0080092","properties":[{"key":"GoTerm","value":"P:regulation of pollen tube growth"},{"key":"GoEvidenceType","value":"IGI:TAIR"}],"evidences":[{"evidenceCode":"ECO:0000316","source":"PubMed","id":"23024212"}]},{"database":"FunFam","id":"1.20.58.2010:FF:000001","properties":[{"key":"EntryName","value":"Rop guanine nucleotide exchange factor 14"},{"key":"MatchStatus","value":"1"}]},{"database":"FunFam","id":"1.20.58.2010:FF:000003","properties":[{"key":"EntryName","value":"Rop guanine nucleotide exchange factor 14"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.58.2010","properties":[{"key":"EntryName","value":"PRONE domain, subdomain 1"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR005512","properties":[{"key":"EntryName","value":"PRONE_dom"}]},{"database":"InterPro","id":"IPR038937","properties":[{"key":"EntryName","value":"RopGEF"}]},{"database":"PANTHER","id":"PTHR33101","properties":[{"key":"EntryName","value":"ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR33101:SF2","properties":[{"key":"EntryName","value":"ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 14"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF03759","properties":[{"key":"EntryName","value":"PRONE"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS51334","properties":[{"key":"EntryName","value":"PRONE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MMLMRRRLACCSREREISIDFDEHEQDKMITYYGLETCIINNQSYEEESGTSRGDGCLTDSLDDDAFSSCSSSKDASSSFSSKWLPMKNDEHSCDGLNLSGRSQHFDAKEKKKQGYGSSQHFDAKEKPGYVYCHLDVEAMKEKFSKLLLGEDVTGGCKGVQVALALSNAVTHLATSIFGELWKLEPLCEEKKQKWRREMDWLLSPTNYMIELVPSKQNDANGRSLEIMTPKARADIHMNLPALQKLDSMLIETLDSMVNTEFWYSEIGSRAEGKNKSTSESKRWWLPSPQVPKPGLSNSGRKKLLDKGKVVYQVFKATKAINENILLEMPVPIVIKEAIPKSGKNSLGDELYKMLAVESATVDEIFISLNLGTEHAALETVNKLESAMFAWKERITEQGSNGKSPVRASWSFAKDPLSEIGRNESLLNRAEALRTQIKSKHPNLPHSFLDATKIQYDKDIGHAVLEAYSRTLANLAFRILSRMGEILKEDSLSNPNSPAPPSCFPSSRDPYRTPERPLLSSRVRHSLTDDMNKADGTETGLDFLFADAKASNSVNTTPSRSSRLWCLSKVPSDTSP","length":576,"molWeight":64434,"crc64":"7DC1C9FC147CC375","md5":"FCC7BF54F5DAF69A08FDF3AEB8830F63"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DOMAIN":1,"SEQUENCE CAUTION":1},"countByFeatureType":{"Chain":1,"Domain":1,"Region":2,"Compositional bias":1,"Modified residue":1,"Sequence conflict":1},"uniParcId":"UPI0000196F70"}}