{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q55688","uniProtkbId":"ALDEC_SYNY3","entryAudit":{"firstPublicDate":"2012-09-05","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1996-11-01","entryVersion":135,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)","taxonId":1111708,"lineage":["Bacteria","Bacillati","Cyanobacteriota","Cyanophyceae","Synechococcales","Merismopediaceae","Synechocystis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"value":"Aldehyde decarbonylase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"value":"AD"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"value":"4.1.99.5"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"value":"Fatty aldehyde decarbonylase"}}]},"genes":[{"orderedLocusNames":[{"value":"sll0208"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20671186"}],"value":"Catalyzes the decarbonylation of fatty aldehydes to alkanes. Requires the presence of ferredoxin, ferredoxin reductase and NADPH for in vitro decarbonylase activity (By similarity). Involved in the biosynthesis of alkanes, mainly heptadecane and pentadecane"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"a long-chain fatty aldehyde + 2 NADPH + O2 + H(+) = a long-chain alkane + formate + 2 NADP(+) + H2O","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21440"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:15740"},{"database":"ChEBI","id":"CHEBI:17176"},{"database":"ChEBI","id":"CHEBI:57783"},{"database":"ChEBI","id":"CHEBI:58349"},{"database":"ChEBI","id":"CHEBI:83563"}],"ecNumber":"4.1.99.5","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}]}},{"commentType":"COFACTOR","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"value":"Binds 2 metal cations per subunit. The catalytic dinuclear metal-binding site could be either a di-iron or a manganese-iron cofactor."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"20671186"}],"value":"Abolishes the presence of alkanes"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"value":"Belongs to the aldehyde decarbonylase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":231,"modifier":"EXACT"}},"description":"Aldehyde decarbonylase","featureId":"PRO_0000418905"},{"type":"Binding site","location":{"start":{"value":32,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"1"}},{"type":"Binding site","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"1"}},{"type":"Binding site","location":{"start":{"value":60,"modifier":"EXACT"},"end":{"value":60,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"2"}},{"type":"Binding site","location":{"start":{"value":63,"modifier":"EXACT"},"end":{"value":63,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"1"}},{"type":"Binding site","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":115,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"2"}},{"type":"Binding site","location":{"start":{"value":147,"modifier":"EXACT"},"end":{"value":147,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:24875"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_00931"}],"ligand":{"name":"Fe cation","id":"ChEBI:CHEBI:24875","label":"2"}},{"type":"Helix","location":{"start":{"value":15,"modifier":"EXACT"},"end":{"value":45,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":47,"modifier":"EXACT"},"end":{"value":49,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":50,"modifier":"EXACT"},"end":{"value":73,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":80,"modifier":"EXACT"},"end":{"value":99,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":103,"modifier":"EXACT"},"end":{"value":107,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":114,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":115,"modifier":"EXACT"},"end":{"value":125,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":128,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":146,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":150,"modifier":"EXACT"},"end":{"value":157,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":159,"modifier":"EXACT"},"end":{"value":190,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":195,"modifier":"EXACT"},"end":{"value":213,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Helix","location":{"start":{"value":217,"modifier":"EXACT"},"end":{"value":224,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]},{"type":"Turn","location":{"start":{"value":225,"modifier":"EXACT"},"end":{"value":227,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Z5S"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0408","category":"Ligand","name":"Iron"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0521","category":"Ligand","name":"NADP"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"8905231","citationType":"journal article","authors":["Kaneko T.","Sato S.","Kotani H.","Tanaka A.","Asamizu E.","Nakamura Y.","Miyajima N.","Hirosawa M.","Sugiura M.","Sasamoto S.","Kimura T.","Hosouchi T.","Matsuno A.","Muraki A.","Nakazaki N.","Naruo K.","Okumura S.","Shimpo S.","Takeuchi C.","Wada T.","Watanabe A.","Yamada M.","Yasuda M.","Tabata S."],"citationCrossReferences":[{"database":"PubMed","id":"8905231"},{"database":"DOI","id":"10.1093/dnares/3.3.109"}],"title":"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions.","publicationDate":"1996","journal":"DNA Res.","firstPage":"109","lastPage":"136","volume":"3"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 27184 / PCC 6803 / Kazusa","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"20671186","citationType":"journal article","authors":["Schirmer A.","Rude M.A.","Li X.","Popova E.","del Cardayre S.B."],"citationCrossReferences":[{"database":"PubMed","id":"20671186"},{"database":"DOI","id":"10.1126/science.1187936"}],"title":"Microbial biosynthesis of alkanes.","publicationDate":"2010","journal":"Science","firstPage":"559","lastPage":"562","volume":"329"},"referencePositions":["FUNCTION","DISRUPTION PHENOTYPE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"BA000022","properties":[{"key":"ProteinId","value":"BAA10217.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S76365","properties":[{"key":"EntryName","value":"S76365"}]},{"database":"PDB","id":"4Z5S","properties":[{"key":"Method","value":"X-ray"},{"key":"Resolution","value":"1.58 A"},{"key":"Chains","value":"A=1-231"}]},{"database":"PDBsum","id":"4Z5S","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q55688","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q55688","properties":[{"key":"Description","value":"-"}]},{"database":"IntAct","id":"Q55688","properties":[{"key":"Interactions","value":"7"}]},{"database":"STRING","id":"1148.gene:10499716","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"1148-gene:10499716","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"BAA10217","properties":[{"key":"ProteinId","value":"BAA10217"},{"key":"GeneId","value":"BAA10217"}]},{"database":"KEGG","id":"syn:sll0208","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1633","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"InParanoid","id":"Q55688","properties":[{"key":"Description","value":"-"}]},{"database":"BRENDA","id":"4.1.99.5","properties":[{"key":"OrganismId","value":"6192"}]},{"database":"Proteomes","id":"UP000001425","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0071771","properties":[{"key":"GoTerm","value":"F:aldehyde oxygenase (deformylating) activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"GO","id":"GO:0046914","properties":[{"key":"GoTerm","value":"F:transition metal ion binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-UniRule"}]},{"database":"CDD","id":"cd00657","properties":[{"key":"EntryName","value":"Ferritin_like"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.20.1260.10","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"HAMAP","id":"MF_00931","properties":[{"key":"EntryName","value":"Aldeh_decarbonylase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR022612","properties":[{"key":"EntryName","value":"Ald_deCOase"}]},{"database":"InterPro","id":"IPR012347","properties":[{"key":"EntryName","value":"Ferritin-like"}]},{"database":"InterPro","id":"IPR009078","properties":[{"key":"EntryName","value":"Ferritin-like_SF"}]},{"database":"NCBIfam","id":"TIGR04059","properties":[{"key":"EntryName","value":"Ald_deCOase"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF11266","properties":[{"key":"EntryName","value":"Ald_deCOase"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF47240","properties":[{"key":"EntryName","value":"Ferritin-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MPELAVRTEFDYSSEIYKDAYSRINAIVIEGEQEAYSNYLQMAELLPEDKEELTRLAKMENRHKKGFQACGNNLQVNPDMPYAQEFFAGLHGNFQHAFSEGKVVTCLLIQALIIEAFAIAAYNIYIPVADDFARKITEGVVKDEYTHLNYGEEWLKANFATAKEELEQANKENLPLVWKMLNQVQGDAKVLGMEKEALVEDFMISYGEALSNIGFSTREIMRMSSYGLAGV","length":231,"molWeight":26178,"crc64":"729215496B3D5BC9","md5":"CE9ABE03344D8E38D58CFB58ACA588B0"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Binding site":6,"Helix":13,"Turn":1},"uniParcId":"UPI00000C0F38"}}