{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q4H4E3","uniProtkbId":"BTRV_NIACI","entryAudit":{"firstPublicDate":"2013-03-06","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-08-30","entryVersion":26,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Niallia circulans","commonName":"Bacillus circulans","taxonId":1397,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Bacillaceae","Niallia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] monooxygenase BtrO"},"ecNumbers":[{"value":"1.14.14.13"}]},"alternativeNames":[{"fullName":{"value":"Butirosin biosynthesis protein V"}}]},"genes":[{"geneName":{"value":"btrV"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15975512"}],"value":"NAD(P)H:FMN oxidoreductase component of a two-component system involved in the biosynthesis of the side chain of the aminoglycoside antibiotics in the biosynthetic pathway of butirosin. Together with BtrO, mediates hydroxylation of gamma-L-Glu-GABA-S-BtrI"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"4-(gamma-L-glutamylamino)butanoyl-[BtrI ACP] + FMNH2 + O2 = 4-(gamma-L-glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI ACP] + FMN + H2O + H(+)","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:53960"},{"database":"Rhea","id":"RHEA-COMP:13743"},{"database":"Rhea","id":"RHEA-COMP:13745"},{"database":"ChEBI","id":"CHEBI:15377"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15379"},{"database":"ChEBI","id":"CHEBI:57618"},{"database":"ChEBI","id":"CHEBI:58210"},{"database":"ChEBI","id":"CHEBI:137998"},{"database":"ChEBI","id":"CHEBI:137999"}],"ecNumber":"1.14.14.13","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15975512"}]}},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"michaelisConstants":[{"constant":6.86,"unit":"uM","substrate":"NADH in the presence of FMN","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15975512"}]},{"constant":7.25,"unit":"uM","substrate":"NADH in the presence of FAD","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15975512"}]}],"note":{"texts":[{"value":"kcat is 1243.13 min(-1) with NADH as substrate in the presence of FMN. kcat is 887.48 min(-1) with NADH as substrate in the presence of FAD."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15975512"}],"value":"Antibiotic biosynthesis; butirosin biosynthesis"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15975512"}],"value":"Homotetramer"}],"commentType":"SUBUNIT"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":82,"modifier":"EXACT"}},"description":"4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] monooxygenase BtrO","featureId":"PRO_0000421728"}],"keywords":[{"id":"KW-0045","category":"Biological process","name":"Antibiotic biosynthesis"},{"id":"KW-0285","category":"Ligand","name":"Flavoprotein"},{"id":"KW-0288","category":"Ligand","name":"FMN"},{"id":"KW-0560","category":"Molecular function","name":"Oxidoreductase"}],"references":[{"referenceNumber":1,"citation":{"id":"16156513","citationType":"journal article","authors":["Kudo F.","Numakura M.","Tamegai H.","Yamamoto H.","Eguchi T.","Kakinuma K."],"citationCrossReferences":[{"database":"PubMed","id":"16156513"},{"database":"DOI","id":"10.1038/ja.2005.47"}],"title":"Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073.","publicationDate":"2005","journal":"J. Antibiot.","firstPage":"373","lastPage":"379","volume":"58"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 21557 / NCIMB 12336 / BU-1709-YQW-B6","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"15975512","citationType":"journal article","authors":["Li Y.","Llewellyn N.M.","Giri R.","Huang F.","Spencer J.B."],"citationCrossReferences":[{"database":"PubMed","id":"15975512"},{"database":"DOI","id":"10.1016/j.chembiol.2005.04.010"}],"title":"Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway.","publicationDate":"2005","journal":"Chem. Biol.","firstPage":"665","lastPage":"675","volume":"12"},"referencePositions":["FUNCTION","CATALYTIC ACTIVITY","PATHWAY","BIOPHYSICOCHEMICAL PROPERTIES","SUBUNIT"],"referenceComments":[{"value":"ATCC 21557 / NCIMB 12336 / BU-1709-YQW-B6","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AB097196","properties":[{"key":"ProteinId","value":"BAE07078.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"AlphaFoldDB","id":"Q4H4E3","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q4H4E3","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00964","properties":[{"key":"RectionId","value":"-"}]},{"database":"GO","id":"GO:0016712","properties":[{"key":"GoTerm","value":"F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"},{"key":"GoEvidenceType","value":"TAS:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000304","source":"PubMed","id":"15975512"}]},{"database":"GO","id":"GO:0017000","properties":[{"key":"GoTerm","value":"P:antibiotic biosynthetic process"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15975512"}]},{"database":"GO","id":"GO:0051289","properties":[{"key":"GoTerm","value":"P:protein homotetramerization"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"15975512"}]},{"database":"InterPro","id":"IPR046135","properties":[{"key":"EntryName","value":"DUF6137"}]},{"database":"Pfam","id":"PF19634","properties":[{"key":"EntryName","value":"DUF6137"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDNKERNTLYQVVYAISGVTGEDGDELVETFKQGPMEVDKRDFFEIVQRVESLFDCTLDMNLEGPYLIHADEIVTKITKLNV","length":82,"molWeight":9416,"crc64":"6456EA01819A7269","md5":"7465C257ACFC599B78551878514C08E3"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"BIOPHYSICOCHEMICAL PROPERTIES":1,"PATHWAY":1,"SUBUNIT":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI0000552E3C"}}