{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q47150","uniProtkbId":"DINJ_ECOLI","entryAudit":{"firstPublicDate":"1997-11-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1997-11-01","entryVersion":151,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Antitoxin DinJ"}}},"genes":[{"geneName":{"value":"dinJ"},"orderedLocusNames":[{"value":"b0226"},{"value":"JW0216"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17263853"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24898247"}],"value":"Antitoxin component of a type II toxin-antitoxin (TA) system (PubMed:17263853). A labile antitoxin that counteracts the effect of cognate toxin YafQ (PubMed:17263853). YafQ and DinJ together bind their own promoter, and repress its expression (PubMed:24898247). There are 2 operators with imperfect inverted repeats (IR) in the dinJ promoter, YafQ-(DinJ)2-YafQ only binds to the first (most upstream) of them to repress transcription; binding to a single IR is sufficient for activity in vivo and in vitro (PubMed:24898247). DinJ alone is as potent a transcriptional repressor as the heterotetramer and also only needs to bind 1 IR to act (PubMed:24898247)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19707553"}],"value":"Cell death governed by the MazE-MazF and DinJ-YafQ TA systems seems to play a role in biofilm formation (PubMed:19707553)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17263853"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19210620"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24898247"}],"value":"Forms a heterotetramer with antitoxin DinJ, with 2 YafQ-DinJ dimers associated via the N-terminus of the DinJ antitoxins (YafQ-(DinJ)2-YafQ) (PubMed:17263853, PubMed:24898247). In this complex the toxin activity is inhibited"}],"commentType":"SUBUNIT"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19210620"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24898247"}],"value":"Not induced by the DNA damaging agent mitomycin C (PubMed:19210620). Transcription is autorepressed by DinJ or YafQ-(DinJ)2-YafQ via operator 1, repressed by LexA via operator 2 (PubMed:24898247)"}],"commentType":"INDUCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19210620"}],"value":"Probably degraded by the Lon and ClpPX proteases in vivo"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"19707553"}],"value":"Cells missing dinJ-yafQ have reduced biofilm formation"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the RelB/DinJ antitoxin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":86,"modifier":"EXACT"}},"description":"Antitoxin DinJ","featureId":"PRO_0000079903"},{"type":"Mutagenesis","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"About 50% loss of promoter repression, probably forms YafQ-DinQ dimers.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24898247"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"About 75% loss of promoter repression, probably forms YafQ-DinQ tetramers.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24898247"}],"alternativeSequence":{}},{"type":"Mutagenesis","location":{"start":{"value":10,"modifier":"EXACT"},"end":{"value":10,"modifier":"EXACT"}},"description":"Nearly complete loss of promoter repression, YafQ-(DinJ)2-YafQ no longer binds DNA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24898247"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Mutagenesis","location":{"start":{"value":35,"modifier":"EXACT"},"end":{"value":35,"modifier":"EXACT"}},"description":"About 90% loss of promoter repression, YafQ-(DinJ)2-YafQ no longer binds DNA.","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"24898247"}],"alternativeSequence":{"originalSequence":"R","alternativeSequences":["A"]}},{"type":"Beta strand","location":{"start":{"value":4,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Q2U"}]},{"type":"Helix","location":{"start":{"value":13,"modifier":"EXACT"},"end":{"value":24,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Q2U"}]},{"type":"Turn","location":{"start":{"value":25,"modifier":"EXACT"},"end":{"value":27,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Q2U"}]},{"type":"Helix","location":{"start":{"value":30,"modifier":"EXACT"},"end":{"value":44,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Q2U"}]},{"type":"Helix","location":{"start":{"value":56,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Q2U"}]},{"type":"Turn","location":{"start":{"value":68,"modifier":"EXACT"},"end":{"value":70,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Q2U"}]},{"type":"Beta strand","location":{"start":{"value":71,"modifier":"EXACT"},"end":{"value":76,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Q2U"}]},{"type":"Helix","location":{"start":{"value":77,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0007829","source":"PDB","id":"4Q2U"}]}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0238","category":"Molecular function","name":"DNA-binding"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0678","category":"Molecular function","name":"Repressor"},{"id":"KW-1277","category":"Biological process","name":"Toxin-antitoxin system"},{"id":"KW-0804","category":"Biological process","name":"Transcription"},{"id":"KW-0805","category":"Biological process","name":"Transcription regulation"}],"references":[{"referenceNumber":1,"citation":{"id":"7596361","citationType":"journal article","authors":["Ohmori H.","Hatada E.","Qiao Y.","Tsuji M.","Fukuda R."],"citationCrossReferences":[{"database":"PubMed","id":"7596361"},{"database":"DOI","id":"10.1016/0165-7992(95)90024-1"}],"title":"dinP, a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologues.","publicationDate":"1995","journal":"Mutat. 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