{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q46824","secondaryAccessions":["Q2M9U5"],"uniProtkbId":"YGFX_ECOLI","entryAudit":{"firstPublicDate":"1998-07-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"1996-11-01","entryVersion":144,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Escherichia coli (strain K12)","taxonId":83333,"lineage":["Bacteria","Pseudomonadati","Pseudomonadota","Gammaproteobacteria","Enterobacterales","Enterobacteriaceae","Escherichia"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Inner membrane protein YgfX"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22239607"}],"value":"Toxin CptA"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22474332"}],"value":"ygfX"},"synonyms":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"22239607"}],"value":"cptA"}],"orderedLocusNames":[{"value":"b2896"},{"value":"JW2864"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22239607"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22474332"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"value":"A probable inner membrane protein. Has been shown not to be a toxin, no effects on growth are seen in LB or minimal medium up to 6 or 21 hours (respectively) after induction of expression (PubMed:23657679). Interacts with cytoskeletal proteins FtsZ and MreB; inhibits FtsZ GTP-dependent polymerization as well as MreB ATP-dependent polymerization (PubMed:22239607). Restores production of prodigiosin antibiotic (Pig) in Serratia strains with deletions of sdhE-ygfX; overexpression of this protein and CptB also restores Pig production to a slightly lesser extent in Serratia (PubMed:22474332)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22239607"}],"value":"Interacts with MreB and FtsZ; interaction with the latter requires FtsZ residues 33-49 (PubMed:22239607)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22239607"}],"value":"Cell inner membrane","id":"SL-0037"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22239607"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22239607"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"23657679"}],"value":"Was originally thought to be the toxic component of a type II toxin-antitoxin (TA) system; overexpression was shown to lead to growth arrest after 5 hours, with initial elongation of cells, followed by swelling (PubMed:22239607). The toxic effects were abrogated by coexpression with antitoxin CptB (now sdhE) (PubMed:22239607). However subsequent studies have been unable to show the toxic effect upon overexpression, nor is the Serratia ortholog a toxin (PubMed:23657679)"}],"commentType":"CAUTION"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"Inner membrane protein YgfX","featureId":"PRO_0000169359"},{"type":"Topological domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":11,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":12,"modifier":"EXACT"},"end":{"value":32,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":33,"modifier":"EXACT"},"end":{"value":37,"modifier":"EXACT"}},"description":"Periplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":38,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Topological domain","location":{"start":{"value":55,"modifier":"EXACT"},"end":{"value":135,"modifier":"EXACT"}},"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"Not required to inhibit FtsZ or MreB polymerization"}],"keywords":[{"id":"KW-0997","category":"Cellular component","name":"Cell inner membrane"},{"id":"KW-1003","category":"Cellular component","name":"Cell membrane"},{"id":"KW-0133","category":"Biological process","name":"Cell shape"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"9278503","citationType":"journal article","authors":["Blattner F.R.","Plunkett G. 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