{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q400C8","secondaryAccessions":["Q8BVM5","Q8K3B9","Q9D121"],"uniProtkbId":"AMZ2_MOUSE","entryAudit":{"firstPublicDate":"2006-01-10","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2005-09-27","entryVersion":129,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"value":"Archaemetzincin-2"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8TXW1"}],"value":"3.4.-.-"}]},"alternativeNames":[{"fullName":{"value":"Archeobacterial metalloproteinase-like protein 2"}}]},"genes":[{"geneName":{"value":"Amz2"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8TXW1"}],"value":"Probable zinc metalloprotease"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Zn(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8TXW1"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29105"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8TXW1"}],"value":"Binds 2 Zn(2+) ions per subunit. One is catalytic, whereas the other seems to have a structural role."}]}},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q400C8-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q400C8-2"],"sequenceIds":["VSP_016984","VSP_016985"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17074343"}],"value":"Predominantly expressed in testis"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"17074343"}],"value":"Expression in testis begins at about postnatal day 10 (P10), with adult level of expression reached at P20"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the peptidase M54 family"}],"commentType":"SIMILARITY"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"AAH25087.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Frameshift","sequence":"BAB23148.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAC35928.1","evidences":[{"evidenceCode":"ECO:0000305"}]},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAC36674.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"Archaemetzincin-2","featureId":"PRO_0000159619"},{"type":"Active site","location":{"start":{"value":255,"modifier":"EXACT"},"end":{"value":255,"modifier":"EXACT"}},"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU10095"}]},{"type":"Binding site","location":{"start":{"value":254,"modifier":"EXACT"},"end":{"value":254,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":258,"modifier":"EXACT"},"end":{"value":258,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":264,"modifier":"EXACT"},"end":{"value":264,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"1","note":"catalytic"}},{"type":"Binding site","location":{"start":{"value":265,"modifier":"EXACT"},"end":{"value":265,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":270,"modifier":"EXACT"},"end":{"value":270,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":289,"modifier":"EXACT"},"end":{"value":289,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Binding site","location":{"start":{"value":292,"modifier":"EXACT"},"end":{"value":292,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29105"}],"evidences":[{"evidenceCode":"ECO:0000250"}],"ligand":{"name":"Zn(2+)","id":"ChEBI:CHEBI:29105","label":"2"}},{"type":"Alternative sequence","location":{"start":{"value":310,"modifier":"EXACT"},"end":{"value":321,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16141072"}],"featureId":"VSP_016984","alternativeSequence":{"originalSequence":"ALVKWIDDESCN","alternativeSequences":["MNEERLQQQLQI"]}},{"type":"Alternative sequence","location":{"start":{"value":322,"modifier":"EXACT"},"end":{"value":359,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"16141072"}],"featureId":"VSP_016985","alternativeSequence":{}}],"keywords":[{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0482","category":"Molecular function","name":"Metalloprotease"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0862","category":"Ligand","name":"Zinc"}],"references":[{"referenceNumber":1,"citation":{"id":"15972818","citationType":"journal article","authors":["Diaz-Perales A.","Quesada V.","Peinado J.R.","Ugalde A.P.","Alvarez J.","Suarez M.F.","Gomis-Rueth X.","Lopez-Otin C."],"citationCrossReferences":[{"database":"PubMed","id":"15972818"},{"database":"DOI","id":"10.1074/jbc.m504533200"}],"title":"Identification and characterization of human archaemetzincin-1 and - 2, two novel members of a family of metalloproteases widely distributed in Archaea.","publicationDate":"2005","journal":"J. Biol. Chem.","firstPage":"30367","lastPage":"30375","volume":"280"},"referencePositions":["RETRACTED PAPER"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"30808005","citationType":"journal article","authors":["Diaz-Perales A.","Quesada V.","Peinado J.R.","Ugalde A.P.","Alvarez J.","Suarez M.F.","Gomis-Rueth F.X.","Lopez-Otin C."],"citationCrossReferences":[{"database":"PubMed","id":"30808005"},{"database":"DOI","id":"10.1074/jbc.w118.007328"}],"publicationDate":"2019","journal":"J. Biol. Chem.","firstPage":"1434","lastPage":"1434","volume":"294"},"referencePositions":["RETRACTION NOTICE OF PUBMED:15972818"]},{"referenceNumber":3,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"},{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":4,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1)"],"referenceComments":[{"value":"129","type":"STRAIN"},{"value":"Mammary tumor","type":"TISSUE"}]},{"referenceNumber":5,"citation":{"id":"17074343","citationType":"journal article","authors":["Lin Y.H.","Lin Y.M.","Teng Y.N.","Hsieh T.Y.","Lin Y.S.","Kuo P.L."],"citationCrossReferences":[{"database":"PubMed","id":"17074343"},{"database":"DOI","id":"10.1016/j.fertnstert.2006.04.039"}],"title":"Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue.","publicationDate":"2006","journal":"Fertil. Steril.","firstPage":"1650","lastPage":"1658","volume":"86"},"referencePositions":["TISSUE SPECIFICITY","DEVELOPMENTAL STAGE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AJ879914","properties":[{"key":"ProteinId","value":"CAI53759.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK004062","properties":[{"key":"ProteinId","value":"BAB23148.1"},{"key":"Status","value":"ALT_FRAME"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK075748","properties":[{"key":"ProteinId","value":"BAC35928.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"AK077195","properties":[{"key":"ProteinId","value":"BAC36674.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC025087","properties":[{"key":"ProteinId","value":"AAH25087.1"},{"key":"Status","value":"ALT_INIT"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001239122.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001252193.1"}]},{"database":"RefSeq","id":"NP_079551.3","properties":[{"key":"NucleotideSequenceId","value":"NM_025275.4"}]},{"database":"AlphaFoldDB","id":"Q400C8","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q400C8","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"199513","properties":[{"key":"Interactions","value":"3"}]},{"database":"FunCoup","id":"Q400C8","properties":[{"key":"Number of interactors","value":"72"}]},{"database":"STRING","id":"10090.ENSMUSP00000159063","properties":[{"key":"Description","value":"-"}]},{"database":"MEROPS","id":"M54.002","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q400C8","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q400C8","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000159063","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"296352","properties":[{"key":"Description","value":"-"}],"isoformId":"Q400C8-1"},{"database":"ProteomicsDB","id":"296353","properties":[{"key":"Description","value":"-"}],"isoformId":"Q400C8-2"},{"database":"Pumba","id":"Q400C8","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"19248","properties":[{"key":"antibodies","value":"73 antibodies from 20 providers"}]},{"database":"DNASU","id":"13929","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000103061.3","properties":[{"key":"ProteinId","value":"ENSMUSP00000099350.3"},{"key":"GeneId","value":"ENSMUSG00000020610.18"}],"isoformId":"Q400C8-1"},{"database":"GeneID","id":"13929","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:13929","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"MGI:104837","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"51321","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:104837","properties":[{"key":"GeneName","value":"Amz2"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000020610","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502QVTZ","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00530000063996","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q400C8","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"CQWLSCV","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"2365600at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"13929","properties":[{"key":"hits","value":"2 hits in 78 CRISPR screens"}]},{"database":"PRO","id":"PR:Q400C8","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 11"}]},{"database":"RNAct","id":"Q400C8","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000020610","properties":[{"key":"ExpressionPatterns","value":"Expressed in seminiferous tubule of testis and 264 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q400C8","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005654","properties":[{"key":"GoTerm","value":"C:nucleoplasm"},{"key":"GoEvidenceType","value":"ISO:GO_Central"}]},{"database":"GO","id":"GO:0008237","properties":[{"key":"GoTerm","value":"F:metallopeptidase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006508","properties":[{"key":"GoTerm","value":"P:proteolysis"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"CDD","id":"cd11375","properties":[{"key":"EntryName","value":"Peptidase_M54"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.40.390.10","properties":[{"key":"EntryName","value":"Collagenase (Catalytic Domain)"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR052009","properties":[{"key":"EntryName","value":"Archaemetzincin"}]},{"database":"InterPro","id":"IPR024079","properties":[{"key":"EntryName","value":"MetalloPept_cat_dom_sf"}]},{"database":"InterPro","id":"IPR012962","properties":[{"key":"EntryName","value":"Pept_M54_archaemetzincn"}]},{"database":"PANTHER","id":"PTHR32205:SF5","properties":[{"key":"EntryName","value":"ARCHAEMETZINCIN-2"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR32205","properties":[{"key":"EntryName","value":"ARCHAEMETZINCIN-2-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF07998","properties":[{"key":"EntryName","value":"Peptidase_M54"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF55486","properties":[{"key":"EntryName","value":"Metalloproteases ('zincins'), catalytic domain"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS00142","properties":[{"key":"EntryName","value":"ZINC_PROTEASE"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MQVLRHSEHTLKTALLSKNPVLVSQYEKLDAGEQRLMNEAFQPRSNLFEPITVHSQSDWISSHPEAPQDFEQFFSDRYRKAPCPKKHIIYIQSIGSLGNTRVISEEYIKWLKGYCEAFFYGLKVKFLEPVSVSETKCSFRVNEHTQNLQIHTGHILAFLKKNKPEDAFCIVGITMIDLYPRDSWNFVFGQASLSSGVGIFSFARYGKDFYTSKYEGNVTSLQLTSPTDYSIFDNYYIPEITSVLLLRSCKTLTHEIGHILGLRHCQWLACLMQGSNHLEESDRRPLNVCPICLRKLQSAIGFNIVERYRALVKWIDDESCNESGATPKSSSEHAHLPKPVEAFKDWREWLMRCIALLEK","length":359,"molWeight":41341,"crc64":"909A226405DBDF4B","md5":"204D33182C6892F9DEFDD43218440E25"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"COFACTOR":1,"ALTERNATIVE PRODUCTS":2,"TISSUE SPECIFICITY":1,"DEVELOPMENTAL STAGE":1,"SIMILARITY":1,"SEQUENCE CAUTION":4},"countByFeatureType":{"Chain":1,"Active site":1,"Binding site":7,"Alternative sequence":2},"uniParcId":"UPI00001E690B"}}