{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q3ZN06","uniProtkbId":"NS1AB_HASV4","entryAudit":{"firstPublicDate":"2008-04-08","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2008-04-08","entryVersion":86,"sequenceVersion":2},"annotationScore":5.0,"organism":{"scientificName":"Human astrovirus-4","commonName":"HAstV-4","taxonId":35300,"lineage":["Viruses","Riboviria","Orthornavirae","Pisuviricota","Stelpaviricetes","Stellavirales","Astroviridae","Mamastrovirus","Mamastrovirus hominis","Mamastrovirus 1"]},"organismHosts":[{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606}],"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Non-structural polyprotein 1AB"}},"contains":[{"recommendedName":{"fullName":{"value":"Protein p19"}}},{"recommendedName":{"fullName":{"value":"Transmembrane protein 1A"}}},{"recommendedName":{"fullName":{"value":"Serine protease p27"},"shortNames":[{"value":"p27"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C6K4"}],"value":"3.4.21.-"}]}},{"recommendedName":{"fullName":{"value":"Viral genome-linked protein"}},"alternativeNames":[{"fullName":{"value":"VPg"}}]},{"recommendedName":{"fullName":{"value":"Protein p20"}}},{"recommendedName":{"fullName":{"value":"RNA-directed RNA polymerase p57"},"shortNames":[{"value":"p57"}],"ecNumbers":[{"value":"2.7.7.48"}]}}]},"genes":[{"geneName":{"value":"ORF1"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C6K4"}],"value":"Responsible for the cleavage of the polyprotein into functional products"}],"commentType":"FUNCTION","molecule":"Serine protease p27"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22787221"},{"evidenceCode":"ECO:0000305","source":"PubMed","id":"22787221"}],"value":"Covalently attached to the 5' extremity of the genomic and subgenomic RNAs (PubMed:22787221). It may serve as a primer for the replicase (Probable)"}],"commentType":"FUNCTION","molecule":"Viral genome-linked protein"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:21248"},{"database":"Rhea","id":"RHEA-COMP:14527"},{"database":"Rhea","id":"RHEA-COMP:17342"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:61557"},{"database":"ChEBI","id":"CHEBI:140395"}],"ecNumber":"2.7.7.48","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00539"}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C6K4"}],"value":"Monomer"}],"commentType":"SUBUNIT","molecule":"Serine protease p27"},{"commentType":"SUBCELLULAR LOCATION","molecule":"Transmembrane protein 1A","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Host membrane","id":"SL-0380"},"topology":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Ribosomal frameshifting"],"isoforms":[{"name":{"value":"nsp1ab"},"isoformIds":["Q3ZN06-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"nsp1a"},"isoformIds":["Q3ZN07-1"],"isoformSequenceStatus":"External"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C6K4"}],"value":"Cleaved by the viral and host proteases (By similarity). The protease is probably autocatalytically cleaved (By similarity)"}],"commentType":"PTM"},{"texts":[{"value":"Generated by a ribosomal frameshift at position 900"}],"commentType":"MISCELLANEOUS","molecule":"Isoform nsp1ab"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the astroviridae polyprotein 1AB family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1415,"modifier":"EXACT"}},"description":"Non-structural polyprotein 1AB","featureId":"PRO_0000327277"},{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":175,"modifier":"EXACT"}},"description":"Protein p19","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000327278"},{"type":"Chain","location":{"start":{"value":176,"modifier":"EXACT"},"end":{"value":419,"modifier":"EXACT"}},"description":"Transmembrane protein 1A","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000327279"},{"type":"Chain","location":{"start":{"value":420,"modifier":"EXACT"},"end":{"value":664,"modifier":"EXACT"}},"description":"Serine protease p27","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000327280"},{"type":"Chain","location":{"start":{"value":665,"modifier":"EXACT"},"end":{"value":755,"modifier":"EXACT"}},"description":"Viral genome-linked protein","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000419587"},{"type":"Chain","location":{"start":{"value":756,"modifier":"EXACT"},"end":{"value":912,"modifier":"EXACT"}},"description":"Protein p20","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000327281"},{"type":"Chain","location":{"start":{"value":913,"modifier":"EXACT"},"end":{"value":1415,"modifier":"EXACT"}},"description":"RNA-directed RNA polymerase p57","evidences":[{"evidenceCode":"ECO:0000255"}],"featureId":"PRO_0000327282"},{"type":"Transmembrane","location":{"start":{"value":154,"modifier":"EXACT"},"end":{"value":174,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":239,"modifier":"EXACT"},"end":{"value":259,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":286,"modifier":"EXACT"},"end":{"value":306,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":313,"modifier":"EXACT"},"end":{"value":333,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":344,"modifier":"EXACT"},"end":{"value":364,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Domain","location":{"start":{"value":1160,"modifier":"EXACT"},"end":{"value":1286,"modifier":"EXACT"}},"description":"RdRp catalytic","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00539"}]},{"type":"Region","location":{"start":{"value":753,"modifier":"EXACT"},"end":{"value":813,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":104,"modifier":"EXACT"},"end":{"value":142,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Coiled coil","location":{"start":{"value":587,"modifier":"EXACT"},"end":{"value":616,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Compositional bias","location":{"start":{"value":783,"modifier":"EXACT"},"end":{"value":795,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":800,"modifier":"EXACT"},"end":{"value":813,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Active site","location":{"start":{"value":461,"modifier":"EXACT"},"end":{"value":461,"modifier":"EXACT"}},"description":"Charge relay system; for serine protease activity","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":489,"modifier":"EXACT"},"end":{"value":489,"modifier":"EXACT"}},"description":"Charge relay system; for serine protease activity","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Active site","location":{"start":{"value":551,"modifier":"EXACT"},"end":{"value":551,"modifier":"EXACT"}},"description":"Charge relay system; for serine protease activity","evidences":[{"evidenceCode":"ECO:0000250"}]},{"type":"Site","location":{"start":{"value":175,"modifier":"EXACT"},"end":{"value":176,"modifier":"EXACT"}},"description":"Cleavage","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Site","location":{"start":{"value":419,"modifier":"EXACT"},"end":{"value":420,"modifier":"EXACT"}},"description":"Cleavage","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Site","location":{"start":{"value":664,"modifier":"EXACT"},"end":{"value":665,"modifier":"EXACT"}},"description":"Cleavage","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"P0C6K4"}]},{"type":"Site","location":{"start":{"value":755,"modifier":"EXACT"},"end":{"value":756,"modifier":"EXACT"}},"description":"Cleavage","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Site","location":{"start":{"value":912,"modifier":"EXACT"},"end":{"value":913,"modifier":"EXACT"}},"description":"Cleavage","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Modified residue","location":{"start":{"value":693,"modifier":"EXACT"},"end":{"value":693,"modifier":"EXACT"}},"description":"O-(5'-phospho-RNA)-tyrosine","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"22787221"}]}],"keywords":[{"id":"KW-0067","category":"Ligand","name":"ATP-binding"},{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0191","category":"PTM","name":"Covalent protein-RNA linkage"},{"id":"KW-0903","category":"Technical term","name":"Direct protein sequencing"},{"id":"KW-1043","category":"Cellular component","name":"Host membrane"},{"id":"KW-0378","category":"Molecular function","name":"Hydrolase"},{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0547","category":"Ligand","name":"Nucleotide-binding"},{"id":"KW-0548","category":"Molecular function","name":"Nucleotidyltransferase"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-0645","category":"Molecular function","name":"Protease"},{"id":"KW-0688","category":"Coding sequence diversity","name":"Ribosomal frameshifting"},{"id":"KW-0696","category":"Molecular function","name":"RNA-directed RNA polymerase"},{"id":"KW-0720","category":"Molecular function","name":"Serine protease"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"},{"id":"KW-0693","category":"Biological process","name":"Viral RNA replication"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-130PKUAVLF754","citationType":"submission","authors":["Barthel J.","Rethwilm A.","Rohayem J."],"title":"Molecular characterization of human astrovirus type 4.","publicationDate":"AUG-2004","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC RNA]"],"referenceComments":[{"value":"Isolate Dresden","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"22787221","citationType":"journal article","authors":["Fuentes C.","Bosch A.","Pinto R.M.","Guix S."],"citationCrossReferences":[{"database":"PubMed","id":"22787221"},{"database":"DOI","id":"10.1128/jvi.00797-12"}],"title":"Identification of human astrovirus genome-linked protein (VPg) essential for viral infectivity.","publicationDate":"2012","journal":"J. Virol.","firstPage":"10070","lastPage":"10078","volume":"86"},"referencePositions":["PROTEIN SEQUENCE OF 699-713","IDENTIFICATION (VIRAL GENOME-LINKED PROTEIN)","FUNCTION (VIRAL GENOME-LINKED PROTEIN)","MUTAGENESIS OF TYR-693; TYR-720; TYR-723; TYR-728; TYR-729 AND TYR-747","COVALENT RNA LINKAGE AT TYR-693"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AY720891","properties":[{"key":"ProteinId","value":"AAW51878.1"},{"key":"Status","value":"ALT_SEQ"},{"key":"MoleculeType","value":"Genomic_RNA"}]},{"database":"SMR","id":"Q3ZN06","properties":[{"key":"Description","value":"-"}]},{"database":"GO","id":"GO:0033644","properties":[{"key":"GoTerm","value":"C:host cell membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0005524","properties":[{"key":"GoTerm","value":"F:ATP binding"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0003723","properties":[{"key":"GoTerm","value":"F:RNA binding"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0003968","properties":[{"key":"GoTerm","value":"F:RNA-directed RNA polymerase activity"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0004252","properties":[{"key":"GoTerm","value":"F:serine-type endopeptidase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0070008","properties":[{"key":"GoTerm","value":"F:serine-type exopeptidase activity"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006351","properties":[{"key":"GoTerm","value":"P:DNA-templated transcription"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006508","properties":[{"key":"GoTerm","value":"P:proteolysis"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"GO","id":"GO:0039694","properties":[{"key":"GoTerm","value":"P:viral RNA genome replication"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0075523","properties":[{"key":"GoTerm","value":"P:viral translational frameshifting"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-KW"}]},{"database":"CDD","id":"cd23172","properties":[{"key":"EntryName","value":"ps-ssRNAv_Astroviridae_RdRp"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.30.70.270","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"2.40.10.10","properties":[{"key":"EntryName","value":"Trypsin-like serine proteases"},{"key":"MatchStatus","value":"2"}]},{"database":"InterPro","id":"IPR045835","properties":[{"key":"EntryName","value":"Astro_1A"}]},{"database":"InterPro","id":"IPR045833","properties":[{"key":"EntryName","value":"Astro_p19"}]},{"database":"InterPro","id":"IPR045836","properties":[{"key":"EntryName","value":"Astro_VPg"}]},{"database":"InterPro","id":"IPR043502","properties":[{"key":"EntryName","value":"DNA/RNA_pol_sf"}]},{"database":"InterPro","id":"IPR022068","properties":[{"key":"EntryName","value":"Mamastrovirus_p20"}]},{"database":"InterPro","id":"IPR009003","properties":[{"key":"EntryName","value":"Peptidase_S1_PA"}]},{"database":"InterPro","id":"IPR043128","properties":[{"key":"EntryName","value":"Rev_trsase/Diguanyl_cyclase"}]},{"database":"InterPro","id":"IPR001205","properties":[{"key":"EntryName","value":"RNA-dir_pol_C"}]},{"database":"InterPro","id":"IPR007094","properties":[{"key":"EntryName","value":"RNA-dir_pol_PSvirus"}]},{"database":"Pfam","id":"PF19415","properties":[{"key":"EntryName","value":"Astro_1A"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF19414","properties":[{"key":"EntryName","value":"Astro_p19"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF19416","properties":[{"key":"EntryName","value":"Astro_VPg"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12285","properties":[{"key":"EntryName","value":"Astrovir_pp_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF00680","properties":[{"key":"EntryName","value":"RdRP_1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13365","properties":[{"key":"EntryName","value":"Trypsin_2"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF56672","properties":[{"key":"EntryName","value":"DNA/RNA polymerases"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF50494","properties":[{"key":"EntryName","value":"Trypsin-like serine proteases"},{"key":"MatchStatus","value":"1"}]},{"database":"PROSITE","id":"PS50507","properties":[{"key":"EntryName","value":"RDRP_SSRNA_POS"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MAHGEPYYSSKPDKDFNFGSTMARRQMTPTMVAKLPNFVRNSPQAYDWIVRGLIFPTTGKTYFQRVVVITGGLEDGTYGSFVFDGREWVEIYPIEHLNLMSSLKLIHKANALQERLRLSQEEKATLALDVQFLQHENVRLKELIPKPEPRKIQMKWIIVGAVLTFLSLIPGGYAQSQINNTIFTDMIAACKYSTETLTENLDLRIKLALANITISDKLDAVRQILNFAFVPRAHWLRTVFYYIHYYEMWNIFMFVLAIGTVMRSARPGTDLITLATSHLSGFRMAVLPTIPFHTTMTLWVMNTLMVCYYFDNLLAITLAILAPILGIIFLCFMEDSNYVSQIRGLIATAVLIAGGHACLTLTGTTTSLFVVILTCRFIRMATVFIGTRFEIRDANGKVVATVPTRIKNVAFDFFQKLKQSGVRVGVNEFVVIKPGALCVIDTPEGKGTGFFSGNDIVTAAHAVGNNTFVNVCYEGLMYEAKVRYMPEKDIAFITCPGDLHPTARLKLSKNPDYSCVTVMAYVNEDLVVSTAAAMVHGNTLSYAVRTQDGMSGAPVCDKYGRVLAVHQTNTGYTGGAVIIDPTDFHPVKAPSRVELLKEEIERLKAQLNSAAENPATAVTQQPVVTLEQKSVSDSDVVDLVRTAMEREMKVLRDEINGILAPFLQKKKGKTKHGRGRVRRNLRKGVKLLTEEEYRELLEKGLDRETFLDLIDRIIGERSGYPDYDDEDYYDEDDDGWGVVGDDVEFDYTEVINFDQAKPTPAPRTVKPKTCPEPEAETQPLDLSQKKEKQLEHEQQVVKSTKPQKNEPQPYSQTYGKAPIWESYDFDWDEDDAKFILPAPHRLTKADEIVLGSKIVKLRTIIETAIKTQNYSALPEAVFELDKAAYEAGLEGFLQRVKSKKGSKKLQRAPEDQGAQNYHSLDAWKSLLEPPRERRCVPANFPLLGHLPINRPIFDDKKPRDDLLGLLPEPTWHAFEEYGPTTWGPQAFIKSFDKFFYAEPIDFFSEYPQLCAFADWATYREFRYLEDTRVIHITATEKNTDSTPAYPKMNYFDTEENYLEAHGWAPYIREFTRVFKGDKPEVLWYLFLKKEIIKEEKIRNSDIRQIVCADPIYTRIGACLEAHQNALMKQHTDTSVGQCGWSPMEGGFKKTMQRLVNKGNKHFIEFDWTRYDGTIPPALFKHIKEIRWNFINKDQREKYKHVHEWYVDNLLNRHVLLPSGEVTLQTRGNPSGQFSTTMDNNMVNFWSQAFEFAYFNGPDKDLWKTYDTVVYGDDRLSTTPSVPDDYEERVINMYRDIFGMWVKPGKVICRDSIVGLSFCGFTVNENLEPVPTSPEKLMASLLKPYKILPDLESLHGKLLCYQLLAAFMAEDHPFKVYVEHCLSRTAKQLRDSGLPARLTEEQLHRIWRGGPKKCDG","length":1415,"molWeight":161278,"crc64":"64A20504EF1736A5","md5":"1253213837F486576FF3CAD80436C397"},"extraAttributes":{"countByCommentType":{"FUNCTION":2,"CATALYTIC ACTIVITY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"PTM":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":7,"Transmembrane":5,"Domain":1,"Region":1,"Coiled coil":2,"Compositional bias":2,"Active site":3,"Site":5,"Modified residue":1},"uniParcId":"UPI000170BA50"}}