{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q3UZD7","secondaryAccessions":["B2RW23"],"uniProtkbId":"GAR1B_MOUSE","entryAudit":{"firstPublicDate":"2007-11-13","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"2005-10-11","entryVersion":103,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:3032524"}],"value":"Golgi-associated RAB2 interactor protein 1B"}},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34714330"}],"value":"Golgi-associated RAB2 interactor-like 1"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34714330"}],"value":"GARI-L1"}]}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:3032524"}],"value":"Garin1b"},"synonyms":[{"value":"Fam137a"},{"evidences":[{"evidenceCode":"ECO:0000312","source":"MGI","id":"MGI:3032524"}],"value":"Fam71f1"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"34714330"}],"value":"Garil1"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34714330"}],"value":"RAB2B effector protein required for accurate acrosome formation and normal male fertility (PubMed:34714330). In complex with RAB2A/RAB2B, seems to suppress excessive vesicle trafficking during acrosome formation (PubMed:34714330)"}],"commentType":"FUNCTION"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34714330"}],"value":"Golgi apparatus","id":"SL-0132"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34714330"}],"value":"Expressed in testis (at protein level)"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34714330"}],"value":"Expressed from day 21, around when spermiogenesis occurs (PubMed:34714330). Expression dramatically increases at the mid-round spermatid stage (steps 4-6) (PubMed:34714330)"}],"commentType":"DEVELOPMENTAL STAGE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"34714330"}],"value":"Mutant males are sterile (PubMed:34714330). Spermatozoa from mutant mice showed abnormal head shapes with an abnormal swollen acrosome morphology and impaired acrosome reaction (PubMed:34714330)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the GARIN family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":341,"modifier":"EXACT"}},"description":"Golgi-associated RAB2 interactor protein 1B","featureId":"PRO_0000311687"}],"keywords":[{"id":"KW-0333","category":"Cellular component","name":"Golgi apparatus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"16141072","citationType":"journal article","authors":["Carninci P.","Kasukawa T.","Katayama S.","Gough J.","Frith M.C.","Maeda N.","Oyama R.","Ravasi T.","Lenhard B.","Wells C.","Kodzius R.","Shimokawa K.","Bajic V.B.","Brenner S.E.","Batalov S.","Forrest A.R.","Zavolan M.","Davis M.J.","Wilming L.G.","Aidinis V.","Allen J.E.","Ambesi-Impiombato A.","Apweiler R.","Aturaliya R.N.","Bailey T.L.","Bansal M.","Baxter L.","Beisel K.W.","Bersano T.","Bono H.","Chalk A.M.","Chiu K.P.","Choudhary V.","Christoffels A.","Clutterbuck D.R.","Crowe M.L.","Dalla E.","Dalrymple B.P.","de Bono B.","Della Gatta G.","di Bernardo D.","Down T.","Engstrom P.","Fagiolini M.","Faulkner G.","Fletcher C.F.","Fukushima T.","Furuno M.","Futaki S.","Gariboldi M.","Georgii-Hemming P.","Gingeras T.R.","Gojobori T.","Green R.E.","Gustincich S.","Harbers M.","Hayashi Y.","Hensch T.K.","Hirokawa N.","Hill D.","Huminiecki L.","Iacono M.","Ikeo K.","Iwama A.","Ishikawa T.","Jakt M.","Kanapin A.","Katoh M.","Kawasawa Y.","Kelso J.","Kitamura H.","Kitano H.","Kollias G.","Krishnan S.P.","Kruger A.","Kummerfeld S.K.","Kurochkin I.V.","Lareau L.F.","Lazarevic D.","Lipovich L.","Liu J.","Liuni S.","McWilliam S.","Madan Babu M.","Madera M.","Marchionni L.","Matsuda H.","Matsuzawa S.","Miki H.","Mignone F.","Miyake S.","Morris K.","Mottagui-Tabar S.","Mulder N.","Nakano N.","Nakauchi H.","Ng P.","Nilsson R.","Nishiguchi S.","Nishikawa S.","Nori F.","Ohara O.","Okazaki Y.","Orlando V.","Pang K.C.","Pavan W.J.","Pavesi G.","Pesole G.","Petrovsky N.","Piazza S.","Reed J.","Reid J.F.","Ring B.Z.","Ringwald M.","Rost B.","Ruan Y.","Salzberg S.L.","Sandelin A.","Schneider C.","Schoenbach C.","Sekiguchi K.","Semple C.A.","Seno S.","Sessa L.","Sheng Y.","Shibata Y.","Shimada H.","Shimada K.","Silva D.","Sinclair B.","Sperling S.","Stupka E.","Sugiura K.","Sultana R.","Takenaka Y.","Taki K.","Tammoja K.","Tan S.L.","Tang S.","Taylor M.S.","Tegner J.","Teichmann S.A.","Ueda H.R.","van Nimwegen E.","Verardo R.","Wei C.L.","Yagi K.","Yamanishi H.","Zabarovsky E.","Zhu S.","Zimmer A.","Hide W.","Bult C.","Grimmond S.M.","Teasdale R.D.","Liu E.T.","Brusic V.","Quackenbush J.","Wahlestedt C.","Mattick J.S.","Hume D.A.","Kai C.","Sasaki D.","Tomaru Y.","Fukuda S.","Kanamori-Katayama M.","Suzuki M.","Aoki J.","Arakawa T.","Iida J.","Imamura K.","Itoh M.","Kato T.","Kawaji H.","Kawagashira N.","Kawashima T.","Kojima M.","Kondo S.","Konno H.","Nakano K.","Ninomiya N.","Nishio T.","Okada M.","Plessy C.","Shibata K.","Shiraki T.","Suzuki S.","Tagami M.","Waki K.","Watahiki A.","Okamura-Oho Y.","Suzuki H.","Kawai J.","Hayashizaki Y."],"citationCrossReferences":[{"database":"PubMed","id":"16141072"},{"database":"DOI","id":"10.1126/science.1112014"}],"title":"The transcriptional landscape of the mammalian genome.","publicationDate":"2005","journal":"Science","firstPage":"1559","lastPage":"1563","volume":"309"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"C57BL/6J","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Testis","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"34714330","citationType":"journal article","authors":["Morohoshi A.","Miyata H.","Oyama Y.","Oura S.","Noda T.","Ikawa M."],"citationCrossReferences":[{"database":"PubMed","id":"34714330"},{"database":"DOI","id":"10.1242/dev.199644"}],"title":"FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice.","publicationDate":"2021","journal":"Development","firstPage":"0","lastPage":"0","volume":"148"},"referencePositions":["FUNCTION","SUBCELLULAR LOCATION","INTERACTION WITH RAB2A AND RAB2B","TISSUE SPECIFICITY","DISRUPTION PHENOTYPE","DEVELOPMENTAL STAGE"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK133908","properties":[{"key":"ProteinId","value":"BAE21920.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC147513","properties":[{"key":"ProteinId","value":"AAI47514.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS39451.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_001276592.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001289663.1"}]},{"database":"RefSeq","id":"NP_001276593.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001289664.1"}]},{"database":"RefSeq","id":"NP_001276594.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001289665.1"}]},{"database":"RefSeq","id":"NP_997141.2","properties":[{"key":"NucleotideSequenceId","value":"NM_207258.3"}]},{"database":"RefSeq","id":"XP_017177142.1","properties":[{"key":"NucleotideSequenceId","value":"XM_017321653.1"}]},{"database":"AlphaFoldDB","id":"Q3UZD7","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q3UZD7","properties":[{"key":"Number of interactors","value":"194"}]},{"database":"STRING","id":"10090.ENSMUSP00000126496","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q3UZD7","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000126496","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"271839","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"17801","properties":[{"key":"antibodies","value":"36 antibodies from 10 providers"}]},{"database":"DNASU","id":"330277","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000090487.12","properties":[{"key":"ProteinId","value":"ENSMUSP00000087973.6"},{"key":"GeneId","value":"ENSMUSG00000039742.16"}]},{"database":"GeneID","id":"330277","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:330277","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc009bdf.1","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:3032524","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"84691","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:3032524","properties":[{"key":"GeneName","value":"Garin1b"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000039742","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502S7XV","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00940000161477","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_069391_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q3UZD7","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"9826157at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"330277","properties":[{"key":"hits","value":"3 hits in 78 CRISPR screens"}]},{"database":"PRO","id":"PR:Q3UZD7","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 6"}]},{"database":"RNAct","id":"Q3UZD7","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000039742","properties":[{"key":"ExpressionPatterns","value":"Expressed in seminiferous tubule of testis and 18 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q3UZD7","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005794","properties":[{"key":"GoTerm","value":"C:Golgi apparatus"},{"key":"GoEvidenceType","value":"IDA:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"34714330"}]},{"database":"GO","id":"GO:0001675","properties":[{"key":"GoTerm","value":"P:acrosome assembly"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"34714330"}]},{"database":"GO","id":"GO:0007340","properties":[{"key":"GoTerm","value":"P:acrosome reaction"},{"key":"GoEvidenceType","value":"IMP:UniProtKB"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"34714330"}]},{"database":"InterPro","id":"IPR022168","properties":[{"key":"EntryName","value":"GARIL-like_Rab2B-bd"}]},{"database":"PANTHER","id":"PTHR22574","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR22574:SF13","properties":[{"key":"EntryName","value":"GOLGI-ASSOCIATED RAB2 INTERACTOR PROTEIN 1B"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF12480","properties":[{"key":"EntryName","value":"GARIL_Rab2_bd"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MMTSVPPRKSWWTSKKTVKVIRSYPTFPSLNAWEKFRGLLPVDGETNPGVGLGVEEGLLCQMVHSPEFNLFPNSVVFESNFVQVRRSRNWKEIYKASNTMALGVTSSVPCLPLPNILLMARVKWHQGQSQTWNRPSRAPSINLKSILPLKFVELQIWDDQERVLRLRTVTEKIYYLKLHPDHPETVFHFWIRLVQILHKGLSITTKDPTILVTHCLVPKSLCSPGGKTELVQKKSKGLQPSESLTHLMAQGESETLSQIFSDLHQQKQYRSEKMHINKTSSEKATPCEDSIPCTCDLNWRDAFMFGEWERENPSGPQPLSLLGTLAASSRPRLSLTGGNSI","length":341,"molWeight":38687,"crc64":"217E10A0E390EA81","md5":"6917703218E054543AB1A46A3682EB58"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBCELLULAR LOCATION":1,"TISSUE SPECIFICITY":1,"DEVELOPMENTAL STAGE":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI00005ABA13"}}