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Acts by antagonizing RUBCN, thereby stimulating phosphatidylinositol 3-kinase activity of the PI3K/PI3KC3 complex (By similarity). Following anchorage to the autophagosomal SNARE STX17, promotes the recruitment of PI3K/PI3KC3 and HOPS complexes to the autophagosome to regulate the fusion specificity of autophagosomes with late endosomes/lysosomes (By similarity). Binds phosphoinositides phosphatidylinositol 3-phosphate (PtdIns(3)P), 4-phosphate (PtdIns(4)P) and 5-phosphate (PtdIns(5)P) (By similarity). In addition to its role in autophagy, acts as a regulator of lipid and glycogen homeostasis (PubMed:30704899). May act as a tumor suppressor (By similarity)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H714"}],"value":"Interacts with UVRAG; the interaction is direct and promotes association with the PI3K/PI3KC3 and HOPS complexes. Interacts with STX17"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H714"}],"value":"Associates with late autophagic structure. Recruitment to autophagosome membrane is promoted by autophagic stimuli"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H714"}],"value":"Cytoplasmic vesicle, autophagosome membrane","id":"SL-0022"},"topology":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H714"}],"value":"Peripheral membrane protein","id":"SL-9903"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H714"}],"value":"Phosphorylated by MTOR at Ser-144 under nutrient-rich conditions. Phosphorylation prevents acetylation by KAT5/TIP60 and impairs RUBCNL/PACER function and autophagosome maturation. Under autophagy induction, Phosphorylation by MTOR is repressed, enabling acetylation by KAT5/TIP60"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q9H714"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30704899"}],"value":"Acetylated by KAT5/TIP60 under autophagy induction, promoting autophagosome maturation and lipid metabolism (PubMed:30704899). Acetylation is prevented by phosphorylation by MTOR (By similarity). Lys-469 and Lys-559 constitute the key sites for tuning function in autophagy (By similarity)"}],"commentType":"PTM"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"30704899"}],"value":"Mice display an increase in the ratio of liver to body weight and significantly lower total ketone bodies in the serum and liver after 24 hours of fasting (PubMed:30704899). Conditional deletion in the liver results in impaired autophagy flux, glycogen and lipid accumulation and liver fibrosis (PubMed:30704899)"}],"commentType":"DISRUPTION PHENOTYPE"},{"commentType":"SEQUENCE CAUTION","sequenceCautionType":"Erroneous initiation","sequence":"BAE41789.1","evidences":[{"evidenceCode":"ECO:0000305"}]}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":648,"modifier":"EXACT"}},"description":"Protein associated with UVRAG as autophagy enhancer","featureId":"PRO_0000350567"},{"type":"Region","location":{"start":{"value":131,"modifier":"EXACT"},"end":{"value":173,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Region","location":{"start":{"value":183,"modifier":"EXACT"},"end":{"value":222,"modifier":"EXACT"}},"description":"Interaction with 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