{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q2YDE7","uniProtkbId":"ARCH_BOVIN","entryAudit":{"firstPublicDate":"2007-05-01","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2007-05-01","entryVersion":105,"sequenceVersion":2},"annotationScore":4.0,"organism":{"scientificName":"Bos taurus","commonName":"Bovine","taxonId":9913,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Laurasiatheria","Artiodactyla","Ruminantia","Pecora","Bovidae","Bovinae","Bos"]},"proteinExistence":"2: Evidence at transcript level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"tRNA-splicing ligase-activating factor archease"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"2.7.7.-"}]}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"ZBTB8OS"}}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"Activation factor of RTCB, the catalytic subunit of the tRNA-splicing ligase complex (tRNA-LC), which is involved in the enzyme-dependent maturation of intron-containing pre-tRNAs. Functions downstream of the tRNA-splicing endonuclease that removes introns, ligating the two generated halves via phosphodiester bond formation. Catalyzes the GTP-dependent activation of RTCB, both proteins forming a composite active site coordinating GTP and metal ions to enable the guanylylation of RTCB a prerequisite to undergo multiple catalytic cycles. Also required for the activation of RTCB in non-canonical, spliceosome-independent, cytoplasmic splicing of XBP1 mRNAs during the unfolded protein response (UPR). Upon endoplasmic reticulum (ER) stress, the endoribonuclease IRE1/ERN1 excises a short intron, generating free exon ends that are aligned by RNA-intrinsic, zipper-like stem-loop structures. These exon ends are then recognized and ligated by RTCB. This splicing event yields the active XBP1s transcription factor, which induces genes required to resolve protein folding defects in the endoplasmic reticulum"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"L-histidyl-[RNA ligase] + GTP = N(pros)-(5'-guanosyl-phosphono)-L-histidyl-[RNA ligase] + diphosphate","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:68084"},{"database":"Rhea","id":"RHEA-COMP:17425"},{"database":"Rhea","id":"RHEA-COMP:17427"},{"database":"ChEBI","id":"CHEBI:29979"},{"database":"ChEBI","id":"CHEBI:33019"},{"database":"ChEBI","id":"CHEBI:37565"},{"database":"ChEBI","id":"CHEBI:176906"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:68085"},"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}]}]},{"commentType":"COFACTOR","cofactors":[{"name":"Mn(2+)","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:29035"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"Binds 1 Mn(2+) ion per subunit. Three Mn(2+) ions are bound in the composite active site formed by RTCB and Archease. Two are exclusively coordinated by RTCB residues and the third one is solely coordinated by ZBTB8OS/Archease residues."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"Monomeric. Could also form homodimers. Associates, as a monomer, with the tRNA-splicing ligase complex composed of the catalytic subunit RTCB and the accessory proteins DDX1, C2orf49/Ashwin/ASW, FAM98B and RTRAF/CGI-99"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"Enriched in the cytoplasm"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"value":"Nucleus","id":"SL-0191"}}]},{"commentType":"ALTERNATIVE PRODUCTS","events":["Alternative splicing"],"isoforms":[{"name":{"value":"1"},"isoformIds":["Q2YDE7-1"],"isoformSequenceStatus":"Displayed"},{"name":{"value":"2"},"isoformIds":["Q2YDE7-2"],"sequenceIds":["VSP_024925"],"isoformSequenceStatus":"Described"}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the archease family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Initiator methionine","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":1,"modifier":"EXACT"}},"description":"Removed","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}]},{"type":"Chain","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"tRNA-splicing ligase-activating factor archease","featureId":"PRO_0000285949"},{"type":"Binding site","location":{"start":{"value":39,"modifier":"EXACT"},"end":{"value":39,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035"}},{"type":"Binding site","location":{"start":{"value":166,"modifier":"EXACT"},"end":{"value":166,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035"}},{"type":"Binding site","location":{"start":{"value":167,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:29035"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}],"ligand":{"name":"Mn(2+)","id":"ChEBI:CHEBI:29035"}},{"type":"Modified residue","location":{"start":{"value":2,"modifier":"EXACT"},"end":{"value":2,"modifier":"EXACT"}},"description":"N-acetylalanine","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q8IWT0"}]},{"type":"Alternative sequence","location":{"start":{"value":140,"modifier":"EXACT"},"end":{"value":167,"modifier":"EXACT"}},"description":"in isoform 2","evidences":[{"evidenceCode":"ECO:0000303","source":"Reference","id":"Ref.1"}],"featureId":"VSP_024925","alternativeSequence":{"originalSequence":"GTEVKAITYSAMQVYNEEKPEVFVIIDI","alternativeSequences":["MPAAAAAASRFSRVRLCVTPYTAAHQAPPSLGFSRELKSKQ"]}}],"keywords":[{"id":"KW-0007","category":"PTM","name":"Acetylation"},{"id":"KW-0025","category":"Coding sequence diversity","name":"Alternative splicing"},{"id":"KW-0106","category":"Ligand","name":"Calcium"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0464","category":"Ligand","name":"Manganese"},{"id":"KW-0479","category":"Ligand","name":"Metal-binding"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0808","category":"Molecular function","name":"Transferase"},{"id":"KW-0819","category":"Biological process","name":"tRNA processing"}],"references":[{"referenceNumber":1,"citation":{"id":"CI-BB3ISCU9UTDT0","citationType":"submission","authoringGroup":["NIH - Mammalian Gene Collection (MGC) project"],"publicationDate":"NOV-2005","submissionDatabase":"EMBL/GenBank/DDBJ databases"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)"],"referenceComments":[{"value":"Crossbred X Angus","type":"STRAIN"},{"value":"Liver","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"BC110257","properties":[{"key":"ProteinId","value":"AAI10258.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"EE251558","properties":[{"key":"ProteinId","value":"-"},{"key":"Status","value":"NOT_ANNOTATED_CDS"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"RefSeq","id":"NP_001069756.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001076288.2"}],"isoformId":"Q2YDE7-2"},{"database":"RefSeq","id":"XP_005203056.1","properties":[{"key":"NucleotideSequenceId","value":"XM_005202999.4"}],"isoformId":"Q2YDE7-1"},{"database":"AlphaFoldDB","id":"Q2YDE7","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q2YDE7","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q2YDE7","properties":[{"key":"Number of interactors","value":"1399"}]},{"database":"STRING","id":"9913.ENSBTAP00000038735","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9913-ENSBTAP00000038735","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSBTAT00000038929.6","properties":[{"key":"ProteinId","value":"ENSBTAP00000038735.3"},{"key":"GeneId","value":"ENSBTAG00000027159.7"}],"isoformId":"Q2YDE7-2"},{"database":"Ensembl","id":"ENSBTAT00000133992.2","properties":[{"key":"ProteinId","value":"ENSBTAP00000092829.1"},{"key":"GeneId","value":"ENSBTAG00000027159.7"}],"isoformId":"Q2YDE7-1"},{"database":"Ensembl","id":"ENSBTAT00085053011","properties":[{"key":"ProteinId","value":"ENSBTAP00085003076"},{"key":"GeneId","value":"ENSBTAG00085031175"}]},{"database":"Ensembl","id":"ENSBTAT00090025077","properties":[{"key":"ProteinId","value":"ENSBTAP00090012937"},{"key":"GeneId","value":"ENSBTAG00090012668"}]},{"database":"GeneID","id":"613795","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"bta:613795","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"339487","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSBTAG00000027159","properties":[{"key":"Description","value":"-"}]},{"database":"VGNC","id":"VGNC:53571","properties":[{"key":"GeneName","value":"ZBTB8OS"}]},{"database":"eggNOG","id":"KOG4528","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000003245","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_111362_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q2YDE7","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"AITYHKM","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"2190767at2759","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000009136","properties":[{"key":"Component","value":"Chromosome 2"}]},{"database":"Bgee","id":"ENSBTAG00000027159","properties":[{"key":"ExpressionPatterns","value":"Expressed in spermatocyte and 108 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q2YDE7","properties":[{"key":"ExpressionPatterns","value":"baseline"}]},{"database":"GO","id":"GO:0005737","properties":[{"key":"GoTerm","value":"C:cytoplasm"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0005634","properties":[{"key":"GoTerm","value":"C:nucleus"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0072669","properties":[{"key":"GoTerm","value":"C:tRNA-splicing ligase complex"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"GO","id":"GO:0044600","properties":[{"key":"GoTerm","value":"F:protein guanylyltransferase activity"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0170057","properties":[{"key":"GoTerm","value":"F:RNA ligase (GTP) activity"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0036498","properties":[{"key":"GoTerm","value":"P:IRE1-mediated unfolded protein response"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0070054","properties":[{"key":"GoTerm","value":"P:mRNA splicing, via endonucleolytic cleavage and ligation"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0000968","properties":[{"key":"GoTerm","value":"P:tRNA exon ligation"},{"key":"GoEvidenceType","value":"IEA:Ensembl"}]},{"database":"GO","id":"GO:0006388","properties":[{"key":"GoTerm","value":"P:tRNA splicing, via endonucleolytic cleavage and ligation"},{"key":"GoEvidenceType","value":"ISS:UniProtKB"}]},{"database":"FunFam","id":"3.55.10.10:FF:000001","properties":[{"key":"EntryName","value":"protein archease isoform X1"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"3.55.10.10","properties":[{"key":"EntryName","value":"Archease domain"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR002804","properties":[{"key":"EntryName","value":"Archease"}]},{"database":"InterPro","id":"IPR023572","properties":[{"key":"EntryName","value":"Archease_dom"}]},{"database":"InterPro","id":"IPR036820","properties":[{"key":"EntryName","value":"Archease_dom_sf"}]},{"database":"PANTHER","id":"PTHR12682","properties":[{"key":"EntryName","value":"ARCHEASE"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR12682:SF11","properties":[{"key":"EntryName","value":"PROTEIN ARCHEASE"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF01951","properties":[{"key":"EntryName","value":"Archease"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF69819","properties":[{"key":"EntryName","value":"MTH1598-like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MALEGEDVRDYNLTEEQKAIKAKYPPVSRKYEYLDHTADVQLHAWGDTLEEAFEQCAMAMFGYMTDTGTVEPLQTIEVETQGDDLQSLLFHFLDEWLYKFSADEFFIPREVKVLNIDQRNFKIRSIGWGEEFSLSKHPQGTEVKAITYSAMQVYNEEKPEVFVIIDI","length":167,"molWeight":19432,"crc64":"124DF476591C13E1","md5":"CEBBBC5B7F8736C01F505D368AE06EF4"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"COFACTOR":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"ALTERNATIVE PRODUCTS":2,"SIMILARITY":1},"countByFeatureType":{"Initiator methionine":1,"Chain":1,"Binding site":3,"Modified residue":1,"Alternative sequence":1},"uniParcId":"UPI00014F7ABC"}}