{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q2M329","secondaryAccessions":["Q8N2I7"],"uniProtkbId":"CF184_HUMAN","entryAudit":{"firstPublicDate":"2006-05-02","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2006-05-02","entryVersion":125,"sequenceVersion":2},"annotationScore":3.0,"organism":{"scientificName":"Homo sapiens","commonName":"Human","taxonId":9606,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Primates","Haplorrhini","Catarrhini","Hominidae","Homo"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Cilia- and flagella-associated protein 184"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"HGNC","id":"HGNC:26900"}],"value":"CFAP184"},"synonyms":[{"value":"CCDC96"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I7M6D6"}],"value":"In complex with CFAP263, acts as a regulator of ciliary beating that connects radial spoke 3 (RS3) to the inner dynein arm (IDA) and the nexin-dynein regulatory complex (N-DRC). The complex is positioned parallel to N-DRC and forms a connection between the arch at the base of RS3, the IDA tail and N-DRC"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I7M6D6"}],"value":"Forms a complex with CFAP263; the interaction is required for functional activity in cilia"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q2M329","intActId":"EBI-12382974"},"interactantTwo":{"uniProtKBAccession":"Q9UBB9","geneName":"TFIP11","intActId":"EBI-1105213"},"numberOfExperiments":6,"organismDiffer":false},{"interactantOne":{"uniProtKBAccession":"Q2M329","intActId":"EBI-12382974"},"interactantTwo":{"uniProtKBAccession":"P09493-10","geneName":"TPM1","intActId":"EBI-12123928"},"numberOfExperiments":3,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I7M6D6"}],"value":"Localizes at cilium but not at the ciliary tips"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"I7M6D6"}],"value":"Cell projection, cilium","id":"SL-0066"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"25074808"}],"value":"Cytoplasm, cytoskeleton, microtubule organizing center, centrosome","id":"SL-0048"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the CFAP184 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":555,"modifier":"EXACT"}},"description":"Cilia- and flagella-associated protein 184","featureId":"PRO_0000234096"},{"type":"Region","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Coiled coil","location":{"start":{"value":305,"modifier":"EXACT"},"end":{"value":441,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Coiled coil","location":{"start":{"value":505,"modifier":"EXACT"},"end":{"value":531,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Compositional bias","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":12,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":41,"modifier":"EXACT"},"end":{"value":54,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":95,"modifier":"EXACT"},"end":{"value":105,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":117,"modifier":"EXACT"}},"description":"Low complexity","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":118,"modifier":"EXACT"},"end":{"value":131,"modifier":"EXACT"}},"description":"Acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":174,"modifier":"EXACT"},"end":{"value":202,"modifier":"EXACT"}},"description":"Basic and acidic residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Natural variant","location":{"start":{"value":96,"modifier":"EXACT"},"end":{"value":96,"modifier":"EXACT"}},"description":"in dbSNP:rs871134","featureCrossReferences":[{"database":"dbSNP","id":"rs871134"}],"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15489334"}],"featureId":"VAR_026162","alternativeSequence":{"originalSequence":"E","alternativeSequences":["K"]}}],"keywords":[{"id":"KW-0002","category":"Technical term","name":"3D-structure"},{"id":"KW-0966","category":"Cellular component","name":"Cell projection"},{"id":"KW-0175","category":"Domain","name":"Coiled coil"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0206","category":"Cellular component","name":"Cytoskeleton"},{"id":"KW-1267","category":"Technical term","name":"Proteomics identification"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"14702039","citationType":"journal article","authors":["Ota T.","Suzuki Y.","Nishikawa T.","Otsuki T.","Sugiyama T.","Irie R.","Wakamatsu A.","Hayashi K.","Sato H.","Nagai K.","Kimura K.","Makita H.","Sekine M.","Obayashi M.","Nishi T.","Shibahara T.","Tanaka T.","Ishii S.","Yamamoto J.","Saito K.","Kawai Y.","Isono Y.","Nakamura Y.","Nagahari K.","Murakami K.","Yasuda T.","Iwayanagi T.","Wagatsuma M.","Shiratori A.","Sudo H.","Hosoiri T.","Kaku Y.","Kodaira H.","Kondo H.","Sugawara M.","Takahashi M.","Kanda K.","Yokoi T.","Furuya T.","Kikkawa E.","Omura Y.","Abe K.","Kamihara K.","Katsuta N.","Sato K.","Tanikawa M.","Yamazaki M.","Ninomiya K.","Ishibashi T.","Yamashita H.","Murakawa K.","Fujimori K.","Tanai H.","Kimata M.","Watanabe M.","Hiraoka S.","Chiba Y.","Ishida S.","Ono Y.","Takiguchi S.","Watanabe S.","Yosida M.","Hotuta T.","Kusano J.","Kanehori K.","Takahashi-Fujii A.","Hara H.","Tanase T.-O.","Nomura Y.","Togiya S.","Komai F.","Hara R.","Takeuchi K.","Arita M.","Imose N.","Musashino K.","Yuuki H.","Oshima A.","Sasaki N.","Aotsuka S.","Yoshikawa Y.","Matsunawa H.","Ichihara T.","Shiohata N.","Sano S.","Moriya S.","Momiyama H.","Satoh N.","Takami S.","Terashima Y.","Suzuki O.","Nakagawa S.","Senoh A.","Mizoguchi H.","Goto Y.","Shimizu F.","Wakebe H.","Hishigaki H.","Watanabe T.","Sugiyama A.","Takemoto M.","Kawakami B.","Yamazaki M.","Watanabe K.","Kumagai A.","Itakura S.","Fukuzumi Y.","Fujimori Y.","Komiyama M.","Tashiro H.","Tanigami A.","Fujiwara T.","Ono T.","Yamada K.","Fujii Y.","Ozaki K.","Hirao M.","Ohmori Y.","Kawabata A.","Hikiji T.","Kobatake N.","Inagaki H.","Ikema Y.","Okamoto S.","Okitani R.","Kawakami T.","Noguchi S.","Itoh T.","Shigeta K.","Senba T.","Matsumura K.","Nakajima Y.","Mizuno T.","Morinaga M.","Sasaki M.","Togashi T.","Oyama M.","Hata H.","Watanabe M.","Komatsu T.","Mizushima-Sugano J.","Satoh T.","Shirai Y.","Takahashi Y.","Nakagawa K.","Okumura K.","Nagase T.","Nomura N.","Kikuchi H.","Masuho Y.","Yamashita R.","Nakai K.","Yada T.","Nakamura Y.","Ohara O.","Isogai T.","Sugano S."],"citationCrossReferences":[{"database":"PubMed","id":"14702039"},{"database":"DOI","id":"10.1038/ng1285"}],"title":"Complete sequencing and characterization of 21,243 full-length human cDNAs.","publicationDate":"2004","journal":"Nat. Genet.","firstPage":"40","lastPage":"45","volume":"36"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"],"referenceComments":[{"value":"Ovary","type":"TISSUE"}]},{"referenceNumber":2,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]","VARIANT LYS-96"],"referenceComments":[{"value":"Brain","type":"TISSUE"}]},{"referenceNumber":3,"citation":{"id":"25074808","citationType":"journal article","authors":["Firat-Karalar E.N.","Sante J.","Elliott S.","Stearns T."],"citationCrossReferences":[{"database":"PubMed","id":"25074808"},{"database":"DOI","id":"10.1242/jcs.157008"}],"title":"Proteomic analysis of mammalian sperm cells identifies new components of the centrosome.","publicationDate":"2014","journal":"J. Cell Sci.","firstPage":"4128","lastPage":"4133","volume":"127"},"referencePositions":["SUBCELLULAR LOCATION"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"AK075056","properties":[{"key":"ProteinId","value":"BAC11373.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC105055","properties":[{"key":"ProteinId","value":"AAI05056.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC112274","properties":[{"key":"ProteinId","value":"AAI12275.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS3395.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_699207.1","properties":[{"key":"NucleotideSequenceId","value":"NM_153376.3"}]},{"database":"PDB","id":"8J07","properties":[{"key":"Method","value":"EM"},{"key":"Resolution","value":"4.10 A"},{"key":"Chains","value":"i1=1-555"}]},{"database":"PDBsum","id":"8J07","properties":[{"key":"Description","value":"-"}]},{"database":"AlphaFoldDB","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"EMDB","id":"EMD-35888","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"129209","properties":[{"key":"Interactions","value":"165"}]},{"database":"FunCoup","id":"Q2M329","properties":[{"key":"Number of interactors","value":"115"}]},{"database":"IntAct","id":"Q2M329","properties":[{"key":"Interactions","value":"167"}]},{"database":"STRING","id":"9606.ENSP00000309285","properties":[{"key":"Description","value":"-"}]},{"database":"CarbonylDB","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"GlyGen","id":"Q2M329","properties":[{"key":"glycosylation","value":"1 site, 1 O-linked glycan (1 site)"}]},{"database":"iPTMnet","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"BioMuta","id":"CCDC96","properties":[{"key":"Description","value":"-"}]},{"database":"DMDM","id":"94707508","properties":[{"key":"Description","value":"-"}]},{"database":"MassIVE","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"9606-ENSP00000309285","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"61364","properties":[{"key":"Description","value":"-"}]},{"database":"Antibodypedia","id":"55414","properties":[{"key":"antibodies","value":"59 antibodies from 15 providers"}]},{"database":"DNASU","id":"257236","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENST00000310085.6","properties":[{"key":"ProteinId","value":"ENSP00000309285.4"},{"key":"GeneId","value":"ENSG00000173013.6"}]},{"database":"GeneID","id":"257236","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"hsa:257236","properties":[{"key":"Description","value":"-"}]},{"database":"MANE-Select","id":"ENST00000310085.6","properties":[{"key":"ProteinId","value":"ENSP00000309285.4"},{"key":"RefSeqNucleotideId","value":"NM_153376.3"},{"key":"RefSeqProteinId","value":"NP_699207.1"}]},{"database":"UCSC","id":"uc003gjv.4","properties":[{"key":"OrganismName","value":"human"}]},{"database":"AGR","id":"HGNC:26900","properties":[{"key":"Description","value":"-"}]},{"database":"ClinPGx","id":"PA144596463","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"257236","properties":[{"key":"Description","value":"-"}]},{"database":"DisGeNET","id":"257236","properties":[{"key":"Description","value":"-"}]},{"database":"GeneCards","id":"CFAP184","properties":[{"key":"Description","value":"-"}]},{"database":"HGNC","id":"HGNC:26900","properties":[{"key":"GeneName","value":"CFAP184"}]},{"database":"HPA","id":"ENSG00000173013","properties":[{"key":"ExpressionPatterns","value":"Tissue enriched (testis)"}]},{"database":"MIM","id":"619347","properties":[{"key":"Type","value":"gene"}]},{"database":"OpenTargets","id":"ENSG00000173013","properties":[{"key":"Description","value":"-"}]},{"database":"VEuPathDB","id":"HostDB:ENSG00000173013","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502QS75","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00950000183337","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_026534_0_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"RLQQKCG","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"10254794at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PAN-GO","id":"Q2M329","properties":[{"key":"Number of GO annotations","value":"3 GO annotations based on evolutionary models"}]},{"database":"PhylomeDB","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"PathwayCommons","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"SignaLink","id":"Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"Agora","id":"ENSG00000173013","properties":[{"key":"NominatedTarget","value":"-"}]},{"database":"BioGRID-ORCS","id":"257236","properties":[{"key":"hits","value":"21 hits in 1153 CRISPR screens"}]},{"database":"GenomeRNAi","id":"257236","properties":[{"key":"Description","value":"-"}]},{"database":"Pharos","id":"Q2M329","properties":[{"key":"DevelopmentLevel","value":"Tdark"}]},{"database":"PRO","id":"PR:Q2M329","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000005640","properties":[{"key":"Component","value":"Chromosome 4"}]},{"database":"RNAct","id":"Q2M329","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSG00000173013","properties":[{"key":"ExpressionPatterns","value":"Expressed in left testis and 107 other cell types or tissues"}]},{"database":"GO","id":"GO:0005930","properties":[{"key":"GoTerm","value":"C:axoneme"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0005813","properties":[{"key":"GoTerm","value":"C:centrosome"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0036064","properties":[{"key":"GoTerm","value":"C:ciliary basal body"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"GO","id":"GO:0060271","properties":[{"key":"GoTerm","value":"P:cilium assembly"},{"key":"GoEvidenceType","value":"IBA:GO_Central"}]},{"database":"InterPro","id":"IPR051885","properties":[{"key":"EntryName","value":"CC_CF"}]},{"database":"InterPro","id":"IPR025254","properties":[{"key":"EntryName","value":"CCDC113/CCDC96_CC"}]},{"database":"PANTHER","id":"PTHR15654","properties":[{"key":"EntryName","value":"COILED-COIL DOMAIN-CONTAINING PROTEIN 113-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR15654:SF1","properties":[{"key":"EntryName","value":"COILED-COIL DOMAIN-CONTAINING PROTEIN 96"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF13870","properties":[{"key":"EntryName","value":"CCDC113_CCDC96_CC"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDVSSEHTKDPGGEGGDGESLAARPSKIKASSGPPTSPEPGELESEPEEEEEEQAASQGGTAADEQAEAPKGLTAAEAAGEEGPGEPGRPAEPQPEPEEPAEVGAEEPAQPEPGAGPEELEAEAGAEELEQAAEGKEVRFQASLPLTRIDEEEAAAAPEAETERVEGEEEDKEETQRDGAESKERDGEGRPAKSQEEGKRLYGRDEFEDLEWSEEVQKLQEQQLRSDLLDQYRSLLVERNRSQRYNLYLQHKIFEALRRKKGLEAAEVADRGAEAEAPEKEQAYLRHLGMLEELKKQQADDLQWYHQELGQLKRQCQEKLTRVEKEWRRFQALKKQVVMQAMGSCRMRGGRQAALREVEQIQALEDKKEKEMSAVRLENIQLKQSLVHFETRMRTQEDLTQGLLLIDFEQLKIENQTFNEKIEERNEELLKLRSKVTNSVQVITHVKEKLHFMDMENACKKTQLAEIEAQAALGRDILTKTKQAREGLRTDNIRLNQKCGLLGKDSLLRDLEEKVDKTELLHRRLESLKRHHASLTLSCRGVRQKIREAKAFLPS","length":555,"molWeight":62711,"crc64":"4C3E430F16287E97","md5":"16E109C2C10035DE21AC93F9C0175030"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":2,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Region":1,"Coiled coil":2,"Compositional bias":6,"Natural variant":1},"uniParcId":"UPI00000705C9"}}