{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q2G2M8","uniProtkbId":"NTDP_STAA8","entryAudit":{"firstPublicDate":"2006-09-05","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2006-03-21","entryVersion":96,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Staphylococcus aureus (strain NCTC 8325 / PS 47)","taxonId":93061,"lineage":["Bacteria","Bacillati","Bacillota","Bacilli","Caryophanales","Staphylococcaceae","Staphylococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"},{"evidenceCode":"ECO:0000305"}],"value":"Nucleoside triphosphate/diphosphate phosphatase"},"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q7A4T2"},{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}],"value":"3.6.1.15"},{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}],"value":"3.6.1.6"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27422825"}],"value":"Nucleoside diphosphatase"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"27422825"}],"value":"NDPase"}]}]},"genes":[{"orderedLocusNames":[{"value":"SAOUHSC_02004"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q7A4T2"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}],"value":"Has nucleoside phosphatase activity towards nucleoside triphosphates and nucleoside diphosphates (By similarity). 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is highest with Mn(2+), followed by Co(2+) (PubMed:27422825). Has low activity with Mg(2+) at high magnesium concentrations (PubMed:27422825). The enzyme probably uses Mg(2+) under physiological conditions (By similarity). Cannot use Cu(2+), Ni(2+), Zn(2+) or Ca(2+) ions (PubMed:27422825)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}],"value":"Hydrolysis of UDP and GDP is inhibited by high concentrations of the substrate (PubMed:27422825). GDP hydrolysis is inhibited by GDP (betaS) and GTP (gammaS), the non-hydrolyzable analogs of GDP and GTP (PubMed:27422825)"}],"commentType":"ACTIVITY REGULATION"},{"commentType":"BIOPHYSICOCHEMICAL PROPERTIES","kineticParameters":{"maximumVelocities":[{"velocity":1.15,"unit":"umol/min/mg","enzyme":"enzyme with ADP as substrate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]},{"velocity":0.985,"unit":"umol/min/mg","enzyme":"enzyme with GDP as substrate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]},{"velocity":2.11,"unit":"umol/min/mg","enzyme":"enzyme with CDP as substrate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]},{"velocity":3.84,"unit":"umol/min/mg","enzyme":"enzyme with UDP as substrate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]},{"velocity":1.88,"unit":"umol/min/mg","enzyme":"enzyme with TDP as substrate","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]}],"michaelisConstants":[{"constant":96.3,"unit":"uM","substrate":"ADP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]},{"constant":102.0,"unit":"uM","substrate":"GDP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]},{"constant":303.0,"unit":"uM","substrate":"CDP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]},{"constant":221.0,"unit":"uM","substrate":"UDP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]},{"constant":203.0,"unit":"uM","substrate":"TDP","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}]}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}],"value":"kcat is 24.9 min(-1) with ADP as substrate. kcat is 21.4 min(-1) with GDP as substrate. kcat is 45.8 min(-1) with CDP as substrate. kcat is 83.3 min(-1) with UDP as substrate. kcat is 40.8 min(-1) with TDP as substrate."}]}}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"}],"value":"Deletion mutant exhibits drastically decreased virulence in a silkworm infection model (PubMed:27422825). It has decreased hemolysin production, nuclease production and colony-spreading ability compared with the parent strain (PubMed:27422825). Deletion of the gene alters the expression of various genes, including the virulence regulatory genes agr, hla, sarZ and sarX, as well as metabolic genes involved in nucleotide metabolism, glycolysis and fermentation pathways (PubMed:27422825)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"27422825"},{"evidenceCode":"ECO:0000305"}],"value":"Activity with nucleoside triphosphates was not detected by Imae et al (PubMed:27422825). However, a later study from Wang et al. in S.aureus strain N315 (AC Q7A4T2) shows that Ntdp can also hydrolyze ATP and GTP (Probable). Wang et al. show that Ntdp is dose-dependent in the ATP hydrolysis process and that the ATP hydrolysis activity is difficult to detect under low protein concentration, probably explaining why the Imae group did not have detected this activity (Probable)"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"},{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Ntdp family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":180,"modifier":"EXACT"}},"description":"Nucleoside triphosphate/diphosphate phosphatase","featureId":"PRO_0000248105"},{"type":"Active site","location":{"start":{"value":26,"modifier":"EXACT"},"end":{"value":26,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}]},{"type":"Binding site","location":{"start":{"value":90,"modifier":"EXACT"},"end":{"value":90,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":106,"modifier":"EXACT"},"end":{"value":106,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":108,"modifier":"EXACT"},"end":{"value":108,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"1"}},{"type":"Binding site","location":{"start":{"value":110,"modifier":"EXACT"},"end":{"value":110,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Binding site","location":{"start":{"value":126,"modifier":"EXACT"},"end":{"value":126,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:18420"}],"evidences":[{"evidenceCode":"ECO:0000255","source":"HAMAP-Rule","id":"MF_01568"}],"ligand":{"name":"Mg(2+)","id":"ChEBI:CHEBI:18420","label":"2"}},{"type":"Mutagenesis","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"Loss of UDP hydrolysis activity. 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