{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q283N4","uniProtkbId":"URAD_MOUSE","entryAudit":{"firstPublicDate":"2008-01-15","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2006-04-04","entryVersion":118,"sequenceVersion":1},"annotationScore":5.0,"organism":{"scientificName":"Mus musculus","commonName":"Mouse","taxonId":10090,"lineage":["Eukaryota","Metazoa","Chordata","Craniata","Vertebrata","Euteleostomi","Mammalia","Eutheria","Euarchontoglires","Glires","Rodentia","Myomorpha","Muroidea","Muridae","Murinae","Mus","Mus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"},"shortNames":[{"value":"OHCU decarboxylase"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16462750"}],"value":"4.1.1.97"}]},"alternativeNames":[{"fullName":{"value":"Parahox neighbor"}},{"fullName":{"value":"Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase"}}]},"genes":[{"geneName":{"value":"Urad"},"synonyms":[{"value":"Prhoxnb"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16462750"}],"value":"Catalyzes the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin, a step in the uric acid degradation pathway following urate oxidation"}],"commentType":"FUNCTION"},{"commentType":"CATALYTIC ACTIVITY","reaction":{"name":"5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+) = (S)-allantoin + CO2","reactionCrossReferences":[{"database":"Rhea","id":"RHEA:26301"},{"database":"ChEBI","id":"CHEBI:15378"},{"database":"ChEBI","id":"CHEBI:15678"},{"database":"ChEBI","id":"CHEBI:16526"},{"database":"ChEBI","id":"CHEBI:58639"}],"ecNumber":"4.1.1.97","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16462750"}]},"physiologicalReactions":[{"directionType":"left-to-right","reactionCrossReference":{"database":"Rhea","id":"RHEA:26302"},"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"16462750"}]}]},{"texts":[{"value":"Purine metabolism; urate degradation; (S)-allantoin from urate: step 3/3"}],"commentType":"PATHWAY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"value":"Homodimer"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Peroxisome","id":"SL-0204"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the OHCU decarboxylase family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":178,"modifier":"EXACT"}},"description":"2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase","featureId":"PRO_0000315241"},{"type":"Active site","location":{"start":{"value":67,"modifier":"EXACT"},"end":{"value":67,"modifier":"EXACT"}},"description":"Proton donor","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}]},{"type":"Binding site","location":{"start":{"value":84,"modifier":"EXACT"},"end":{"value":84,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58639"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"ligand":{"name":"5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate","id":"ChEBI:CHEBI:58639"}},{"type":"Binding site","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15678"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"ligand":{"name":"(S)-allantoin","id":"ChEBI:CHEBI:15678"}},{"type":"Binding site","location":{"start":{"value":87,"modifier":"EXACT"},"end":{"value":87,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58639"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"ligand":{"name":"5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate","id":"ChEBI:CHEBI:58639"}},{"type":"Binding site","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15678"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"ligand":{"name":"(S)-allantoin","id":"ChEBI:CHEBI:15678"}},{"type":"Binding site","location":{"start":{"value":88,"modifier":"EXACT"},"end":{"value":88,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58639"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"ligand":{"name":"5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate","id":"ChEBI:CHEBI:58639"}},{"type":"Binding site","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":121,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15678"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"ligand":{"name":"(S)-allantoin","id":"ChEBI:CHEBI:15678"}},{"type":"Binding site","location":{"start":{"value":121,"modifier":"EXACT"},"end":{"value":121,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:58639"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"ligand":{"name":"5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate","id":"ChEBI:CHEBI:58639"}},{"type":"Binding site","location":{"start":{"value":123,"modifier":"EXACT"},"end":{"value":123,"modifier":"EXACT"}},"description":"","featureCrossReferences":[{"database":"ChEBI","id":"CHEBI:15678"}],"evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"A1L259"}],"ligand":{"name":"(S)-allantoin","id":"ChEBI:CHEBI:15678"}}],"keywords":[{"id":"KW-0210","category":"Molecular function","name":"Decarboxylase"},{"id":"KW-0456","category":"Molecular function","name":"Lyase"},{"id":"KW-0576","category":"Cellular component","name":"Peroxisome"},{"id":"KW-0659","category":"Biological process","name":"Purine metabolism"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"16462750","citationType":"journal article","authors":["Ramazzina I.","Folli C.","Secchi A.","Berni R.","Percudani R."],"citationCrossReferences":[{"database":"PubMed","id":"16462750"},{"database":"DOI","id":"10.1038/nchembio768"}],"title":"Completing the uric acid degradation pathway through phylogenetic comparison of whole genomes.","publicationDate":"2006","journal":"Nat. Chem. Biol.","firstPage":"144","lastPage":"148","volume":"2"},"referencePositions":["NUCLEOTIDE SEQUENCE [MRNA]","FUNCTION","CATALYTIC ACTIVITY"]},{"referenceNumber":2,"citation":{"id":"15489334","citationType":"journal article","authoringGroup":["The MGC Project Team"],"citationCrossReferences":[{"database":"PubMed","id":"15489334"},{"database":"DOI","id":"10.1101/gr.2596504"}],"title":"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).","publicationDate":"2004","journal":"Genome Res.","firstPage":"2121","lastPage":"2127","volume":"14"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]"]},{"referenceNumber":3,"citation":{"id":"21183079","citationType":"journal article","authors":["Huttlin E.L.","Jedrychowski M.P.","Elias J.E.","Goswami T.","Rad R.","Beausoleil S.A.","Villen J.","Haas W.","Sowa M.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"21183079"},{"database":"DOI","id":"10.1016/j.cell.2010.12.001"}],"title":"A tissue-specific atlas of mouse protein phosphorylation and expression.","publicationDate":"2010","journal":"Cell","firstPage":"1174","lastPage":"1189","volume":"143"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"Liver","type":"TISSUE"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"DQ225767","properties":[{"key":"ProteinId","value":"ABB46374.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"EMBL","id":"BC152959","properties":[{"key":"ProteinId","value":"AAI52960.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"mRNA"}]},{"database":"CCDS","id":"CCDS39399.1","properties":[{"key":"Description","value":"-"}]},{"database":"RefSeq","id":"NP_001034767.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001039678.2"}]},{"database":"AlphaFoldDB","id":"Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q283N4","properties":[{"key":"Number of interactors","value":"148"}]},{"database":"STRING","id":"10090.ENSMUSP00000098000","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"PhosphoSitePlus","id":"Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"SwissPalm","id":"Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"jPOST","id":"Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"10090-ENSMUSP00000098000","properties":[{"key":"Description","value":"-"}]},{"database":"ProteomicsDB","id":"298255","properties":[{"key":"Description","value":"-"}]},{"database":"DNASU","id":"231903","properties":[{"key":"Description","value":"-"}]},{"database":"Ensembl","id":"ENSMUST00000100433.5","properties":[{"key":"ProteinId","value":"ENSMUSP00000098000.4"},{"key":"GeneId","value":"ENSMUSG00000075543.5"}]},{"database":"GeneID","id":"231903","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mmu:231903","properties":[{"key":"Description","value":"-"}]},{"database":"UCSC","id":"uc009aoa.2","properties":[{"key":"OrganismName","value":"mouse"}]},{"database":"AGR","id":"MGI:3647519","properties":[{"key":"Description","value":"-"}]},{"database":"CTD","id":"646625","properties":[{"key":"Description","value":"-"}]},{"database":"MGI","id":"MGI:3647519","properties":[{"key":"GeneName","value":"Urad"}]},{"database":"VEuPathDB","id":"HostDB:ENSMUSG00000075543","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"KOG0848","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"GeneTree","id":"ENSGT00390000002395","properties":[{"key":"Description","value":"-"}]},{"database":"HOGENOM","id":"CLU_092522_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"RCQNERA","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"9970124at2759","properties":[{"key":"Description","value":"-"}]},{"database":"PhylomeDB","id":"Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"UniPathway","id":"UPA00394","properties":[{"key":"RectionId","value":"UER00652"}]},{"database":"BioGRID-ORCS","id":"231903","properties":[{"key":"hits","value":"3 hits in 76 CRISPR screens"}]},{"database":"PRO","id":"PR:Q283N4","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000000589","properties":[{"key":"Component","value":"Chromosome 5"}]},{"database":"RNAct","id":"Q283N4","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"Bgee","id":"ENSMUSG00000075543","properties":[{"key":"ExpressionPatterns","value":"Expressed in embryonic cell in blastocyst and 14 other cell types or tissues"}]},{"database":"ExpressionAtlas","id":"Q283N4","properties":[{"key":"ExpressionPatterns","value":"baseline and differential"}]},{"database":"GO","id":"GO:0005777","properties":[{"key":"GoTerm","value":"C:peroxisome"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0051997","properties":[{"key":"GoTerm","value":"F:2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0016831","properties":[{"key":"GoTerm","value":"F:carboxy-lyase activity"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0006154","properties":[{"key":"GoTerm","value":"P:adenosine catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0000255","properties":[{"key":"GoTerm","value":"P:allantoin metabolic process"},{"key":"GoEvidenceType","value":"IEA:InterPro"}]},{"database":"GO","id":"GO:0006196","properties":[{"key":"GoTerm","value":"P:AMP catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0006157","properties":[{"key":"GoTerm","value":"P:deoxyadenosine catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0006161","properties":[{"key":"GoTerm","value":"P:deoxyguanosine catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0006149","properties":[{"key":"GoTerm","value":"P:deoxyinosine catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0046055","properties":[{"key":"GoTerm","value":"P:dGMP catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0046038","properties":[{"key":"GoTerm","value":"P:GMP catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0006147","properties":[{"key":"GoTerm","value":"P:guanine catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0009114","properties":[{"key":"GoTerm","value":"P:hypoxanthine catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0006204","properties":[{"key":"GoTerm","value":"P:IMP catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0006148","properties":[{"key":"GoTerm","value":"P:inosine catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0019628","properties":[{"key":"GoTerm","value":"P:urate catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"GO","id":"GO:0009115","properties":[{"key":"GoTerm","value":"P:xanthine catabolic process"},{"key":"GoEvidenceType","value":"IDA:MGI"}],"evidences":[{"evidenceCode":"ECO:0000314","source":"PubMed","id":"16462750"}]},{"database":"FunFam","id":"1.10.3330.10:FF:000001","properties":[{"key":"EntryName","value":"2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"},{"key":"MatchStatus","value":"1"}]},{"database":"Gene3D","id":"1.10.3330.10","properties":[{"key":"EntryName","value":"Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR018020","properties":[{"key":"EntryName","value":"OHCU_decarboxylase"}]},{"database":"InterPro","id":"IPR017580","properties":[{"key":"EntryName","value":"OHCU_decarboxylase-1"}]},{"database":"InterPro","id":"IPR036778","properties":[{"key":"EntryName","value":"OHCU_decarboxylase_sf"}]},{"database":"NCBIfam","id":"TIGR03164","properties":[{"key":"EntryName","value":"UHCUDC"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43466","properties":[{"key":"EntryName","value":"2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR43466:SF1","properties":[{"key":"EntryName","value":"2-OXO-4-HYDROXY-4-CARBOXY-5-UREIDOIMIDAZOLINE DECARBOXYLASE-RELATED"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF09349","properties":[{"key":"EntryName","value":"OHCU_decarbox"},{"key":"MatchStatus","value":"1"}]},{"database":"SUPFAM","id":"SSF158694","properties":[{"key":"EntryName","value":"UraD-Like"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MDMVKVNSMDFGEFVDVFGNIVEKCPLIAAAVWSQRPFSGLEDLENHFFAFIDALPRSGQEGILRCHPDLAGRDLQQGTLTAESQREQSQAGLTSLDTDDRLRLQQLNAQYRERFGFPFVLAARLSDRATVPRELARRLQCQPESELRTALGEVKKISHLRLTDLLGAHSHSARVELP","length":178,"molWeight":20017,"crc64":"FDA1941E8BA89293","md5":"DFE8AD6780CFC76F0E7578CD9C986295"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"CATALYTIC ACTIVITY":1,"PATHWAY":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Active site":1,"Binding site":8},"uniParcId":"UPI00001C366C"}}