{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q1D3H0","uniProtkbId":"BACN_MYXXD","entryAudit":{"firstPublicDate":"2024-05-29","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2006-07-11","entryVersion":94,"sequenceVersion":1},"annotationScore":3.0,"organism":{"scientificName":"Myxococcus xanthus (strain DK1622)","taxonId":246197,"lineage":["Bacteria","Pseudomonadati","Myxococcota","Myxococcia","Myxococcales","Cystobacterineae","Myxococcaceae","Myxococcus"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29180656"}],"value":"Bactofilin BacN"}}},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"29180656"}],"value":"bacN"},"orderedLocusNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ABF92206.1"}],"value":"MXAN_4637"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29180656"}],"value":"A non-essential component of the chromosome segregation machinery (PubMed:29180656). Positions the ParA-ParB-parS chromosome segregation machinery within the cell; BacP seems to be the most important bactofilin in this process (PubMed:29180656). Forms a heteropolymeric, subpolar scaffold in the cell; BacP probably forms the core, BacO contributes to position and integrity while BacN does not seem to contribute to assembly (PubMed:29180656)"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29180656"}],"value":"Interacts with BacO and BacP, the 3 proteins colocalize as an extended structure (PubMed:29180656)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29180656"}],"value":"Forms subpolar patches 1-2 um in length; 1 mature patch and one nascent patch in new cells. As cells elongate the nascent patch grows and a new one assembles at midcell, which splits upon division (PubMed:29180656). The patches colocalize with ParB (PubMed:29180656)"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29180656"}],"value":"Cytoplasm, cytoskeleton","id":"SL-0090"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"29180656"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"32738827"}],"value":"BacP has nearly wild-type localization, only minor changes in ParA or ParB-parS location, no changes for PadC (PubMed:29180656). In a triple bacpN-bacO-bacP deletion ParA, ParB-parS and PadC are mislocalized, nucleoids are more compact, DNA origin regions are mislocalized, DNA content increases (PubMed:29180656, PubMed:32738827). The triple bacNOP deletion is synthetically lethal with smc or double scpAB deletions (PubMed:32738827)"}],"commentType":"DISRUPTION PHENOTYPE"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305","source":"PubMed","id":"29180656"}],"value":"Belongs to the bactofilin family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":113,"modifier":"EXACT"}},"description":"Bactofilin BacN","featureId":"PRO_0000460327"}],"keywords":[{"id":"KW-0159","category":"Biological process","name":"Chromosome partition"},{"id":"KW-0963","category":"Cellular component","name":"Cytoplasm"},{"id":"KW-0206","category":"Cellular component","name":"Cytoskeleton"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"}],"references":[{"referenceNumber":1,"citation":{"id":"17015832","citationType":"journal article","authors":["Goldman B.S.","Nierman W.C.","Kaiser D.","Slater S.C.","Durkin A.S.","Eisen J.A.","Ronning C.M.","Barbazuk W.B.","Blanchard M.","Field C.","Halling C.","Hinkle G.","Iartchuk O.","Kim H.S.","Mackenzie C.","Madupu R.","Miller N.","Shvartsbeyn A.","Sullivan S.A.","Vaudin M.","Wiegand R.","Kaplan H.B."],"citationCrossReferences":[{"database":"PubMed","id":"17015832"},{"database":"DOI","id":"10.1073/pnas.0607335103"}],"title":"Evolution of sensory complexity recorded in a myxobacterial genome.","publicationDate":"2006","journal":"Proc. Natl. Acad. Sci. U.S.A.","firstPage":"15200","lastPage":"15205","volume":"103"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"DK1622","type":"STRAIN"}],"evidences":[{"evidenceCode":"ECO:0000312","source":"EMBL","id":"ABF92206.1"}]},{"referenceNumber":2,"citation":{"id":"29180656","citationType":"journal article","authors":["Lin L.","Osorio Valeriano M.","Harms A.","Soegaard-Andersen L.","Thanbichler M."],"citationCrossReferences":[{"database":"PubMed","id":"29180656"},{"database":"DOI","id":"10.1038/s41467-017-02015-z"}],"title":"Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus.","publicationDate":"2017","journal":"Nat. Commun.","firstPage":"1817","lastPage":"1817","volume":"8"},"referencePositions":["FUNCTION","SUBUNIT","SUBCELLULAR LOCATION","DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"DK1622","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"32738827","citationType":"journal article","authors":["Anand D.","Schumacher D.","Soegaard-Andersen L."],"citationCrossReferences":[{"database":"PubMed","id":"32738827"},{"database":"DOI","id":"10.1111/mmi.14583"}],"title":"SMC and the bactofilin/PadC scaffold have distinct yet redundant functions in chromosome segregation and organization in Myxococcus xanthus.","publicationDate":"2020","journal":"Mol. Microbiol.","firstPage":"839","lastPage":"856","volume":"114"},"referencePositions":["DISRUPTION PHENOTYPE"],"referenceComments":[{"value":"DK1622","type":"STRAIN"}]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"CP000113","properties":[{"key":"ProteinId","value":"ABF92206.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"RefSeq","id":"WP_002636699.1","properties":[{"key":"NucleotideSequenceId","value":"NC_008095.1"}]},{"database":"AlphaFoldDB","id":"Q1D3H0","properties":[{"key":"Description","value":"-"}]},{"database":"SMR","id":"Q1D3H0","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"246197.MXAN_4637","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblBacteria","id":"ABF92206","properties":[{"key":"ProteinId","value":"ABF92206"},{"key":"GeneId","value":"MXAN_4637"}]},{"database":"GeneID","id":"41361937","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"mxa:MXAN_4637","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"COG1664","properties":[{"key":"ToxonomicScope","value":"Bacteria"}]},{"database":"HOGENOM","id":"CLU_072799_4_3_7","properties":[{"key":"Description","value":"-"}]},{"database":"OrthoDB","id":"119922at2","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002402","properties":[{"key":"Component","value":"Chromosome"}]},{"database":"GO","id":"GO:0005856","properties":[{"key":"GoTerm","value":"C:cytoskeleton"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"Gene3D","id":"2.160.20.120","properties":[{"key":"EntryName","value":"-"},{"key":"MatchStatus","value":"1"}]},{"database":"InterPro","id":"IPR007607","properties":[{"key":"EntryName","value":"BacA/B"}]},{"database":"PANTHER","id":"PTHR35024","properties":[{"key":"EntryName","value":"HYPOTHETICAL CYTOSOLIC PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR35024:SF4","properties":[{"key":"EntryName","value":"POLYMER-FORMING CYTOSKELETAL PROTEIN"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF04519","properties":[{"key":"EntryName","value":"Bactofilin"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MATGETGIIGKGIVIKGNLTGGGDLVIEGRVEGQIALKNHLTIESTGKVQADIRAEELTINGEASGNIDASSRVAINASAKVAGDIKAPRVVIEDGAVFNGSIEMDVRLPDDI","length":113,"molWeight":11595,"crc64":"7DB12DD9F7DC388D","md5":"021D1E3EDFF59AA39D26B996E3521908"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"SUBCELLULAR LOCATION":1,"DISRUPTION PHENOTYPE":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1},"uniParcId":"UPI0000006582"}}