{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q19614","uniProtkbId":"SLFL2_CAEEL","entryAudit":{"firstPublicDate":"2021-04-07","lastAnnotationUpdateDate":"2026-09-02","lastSequenceUpdateDate":"2003-10-01","entryVersion":155,"sequenceVersion":3},"annotationScore":5.0,"organism":{"scientificName":"Caenorhabditis elegans","taxonId":6239,"evidences":[{"evidenceCode":"ECO:0000312","source":"Proteomes","id":"UP000001940"}],"lineage":["Eukaryota","Metazoa","Ecdysozoa","Nematoda","Chromadorea","Rhabditida","Rhabditina","Rhabditomorpha","Rhabditoidea","Rhabditidae","Peloderinae","Caenorhabditis"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37758951"}],"value":"Schlafen-like protein 2"},"shortNames":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37758951"}],"value":"SLFL-2"},{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37758951"}],"value":"SLFN-like 2"}],"ecNumbers":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37758951"}],"value":"3.1.-.-"}]},"alternativeNames":[{"fullName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"F20A1.9"}],"value":"21U-RNA biogenesis fouled up protein 2"}},{"fullName":{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"PUCH complex member slfl-2"}},{"fullName":{"value":"Protein tofu-2"}}]},"genes":[{"geneName":{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"F20A1.9"}],"value":"tofu-2"},"orfNames":[{"evidences":[{"evidenceCode":"ECO:0000312","source":"WormBase","id":"F20A1.9"}],"value":"F20A1.9"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37758951"}],"value":"Component of the trimeric PUCH (precursor of 21U RNA 5'-end cleavage holoenzyme) complex, that acts as an endoribonuclease processing the 5'-end of precursor Piwi-interacting RNAs (piRNAs) (PubMed:37758951). The PUCH complex consists of tofu-1, tofu-2 and either slfl-3 or slfl-4, with tofu-2 exhibiting endoribonuclease activity (PubMed:37758951). PUCH-mediated processing strictly requires a 7-methyl-G cap (m7 G-cap) and an uracil at position three (U3) (PubMed:37758951). PUCH also exhibits a strict bias for piRNA precursors with an A or G at position 1 (PubMed:37758951). Mature piRNA production is enhanced by the interaction of PUCH with the PETISCO complex, which is stabilizing piRNA precursors and allows their processing by PUCH (PubMed:37758951)"}],"commentType":"FUNCTION"},{"commentType":"COFACTOR","cofactors":[{"name":"Mg(2+)","evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37758951"}],"cofactorCrossReference":{"database":"ChEBI","id":"CHEBI:18420"}}],"note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37758951"}],"value":"Can also use Mn(2+) or Ca(2+), but not Zn(2+)."}]}},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37758951"}],"value":"Inhibited by ethylenediaminetetraacetic acid (EDTA)"}],"commentType":"ACTIVITY REGULATION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37758951"}],"value":"Component of the trimeric PUCH (precursor of 21U RNA 5'-end cleavage holoenzyme) complex; consisting of tofu-1, tofu-2 and either slfl-3 or slfl-4 (PubMed:37758951). Within the complex, interacts (via N-terminus) with tofu-1 (via N-terminus); the interaction stabilizes tofu-2 and may form a functional nuclease (PubMed:37758951). Within the complex, interacts (via N-terminus) with slfl-3 (via N-terminus); the presence of tofu-1 is required for this interaction (PubMed:37758951)"}],"commentType":"SUBUNIT"},{"commentType":"SUBCELLULAR LOCATION","note":{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37758951"}],"value":"The localization to the mitochondrion is mediated by slfl-3"}]},"subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31216475"}],"value":"Cytoplasm","id":"SL-0086"}},{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"37758951"}],"value":"Mitochondrion","id":"SL-0173"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"31216475"}],"value":"Expressed in the germline"}],"commentType":"TISSUE SPECIFICITY"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37758951"}],"value":"In mammalian Schlafen proteins, two SLFN-folds come together to form the nuclease domain. In tofu-1 and tofu-2, only one SLFN-like fold can be identified, suggesting that the two proteins may interact via their SLFN-like folds to form a functional nuclease"}],"commentType":"DOMAIN"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the Schlafen family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":433,"modifier":"EXACT"}},"description":"Schlafen-like protein 2","featureId":"PRO_0000452468"},{"type":"Domain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":168,"modifier":"EXACT"}},"description":"B30.2/SPRY","evidences":[{"evidenceCode":"ECO:0000255","source":"PROSITE-ProRule","id":"PRU00548"}]},{"type":"Region","location":{"start":{"value":199,"modifier":"EXACT"},"end":{"value":400,"modifier":"EXACT"}},"description":"SLFN-like fold","evidences":[{"evidenceCode":"ECO:0000303","source":"PubMed","id":"37758951"}]},{"type":"Active site","location":{"start":{"value":211,"modifier":"EXACT"},"end":{"value":211,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q5U311"}]},{"type":"Active site","location":{"start":{"value":216,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"","evidences":[{"evidenceCode":"ECO:0000250","source":"UniProtKB","id":"Q5U311"}]},{"type":"Mutagenesis","location":{"start":{"value":216,"modifier":"EXACT"},"end":{"value":216,"modifier":"EXACT"}},"description":"Loss of piRNA precursor processing. 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