{"entryType":"UniProtKB reviewed (Swiss-Prot)","primaryAccession":"Q12066","secondaryAccessions":["D6VRQ9"],"uniProtkbId":"NUR1_YEAST","entryAudit":{"firstPublicDate":"2006-01-10","lastAnnotationUpdateDate":"2026-06-10","lastSequenceUpdateDate":"1996-11-01","entryVersion":164,"sequenceVersion":1},"annotationScore":4.0,"organism":{"scientificName":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","commonName":"Baker's yeast","taxonId":559292,"lineage":["Eukaryota","Fungi","Dikarya","Ascomycota","Saccharomycotina","Saccharomycetes","Saccharomycetales","Saccharomycetaceae","Saccharomyces"]},"proteinExistence":"1: Evidence at protein level","proteinDescription":{"recommendedName":{"fullName":{"value":"Nuclear rim protein 1"}}},"genes":[{"geneName":{"value":"NUR1"},"orderedLocusNames":[{"value":"YDL089W"}],"orfNames":[{"value":"D2416"}]}],"comments":[{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18997772"}],"value":"Member of a perinuclear network that controls recombination at multiple loci to maintain genome stability. Required for rDNA repeat stability"}],"commentType":"FUNCTION"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"15023545"},{"evidenceCode":"ECO:0000269","source":"PubMed","id":"18997772"}],"value":"Interacts with CSM1"}],"commentType":"SUBUNIT"},{"commentType":"INTERACTION","interactions":[{"interactantOne":{"uniProtKBAccession":"Q12066","intActId":"EBI-35877"},"interactantTwo":{"uniProtKBAccession":"Q03707","geneName":"SRC1","intActId":"EBI-18064"},"numberOfExperiments":2,"organismDiffer":false}]},{"commentType":"SUBCELLULAR LOCATION","subcellularLocations":[{"location":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562095"}],"value":"Nucleus membrane","id":"SL-0182"},"topology":{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562095"}],"value":"Multi-pass membrane protein","id":"SL-9909"}}]},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000269","source":"PubMed","id":"14562106"}],"value":"Present with 606 molecules/cell in log phase SD medium"}],"commentType":"MISCELLANEOUS"},{"texts":[{"evidences":[{"evidenceCode":"ECO:0000305"}],"value":"Belongs to the NUR1 family"}],"commentType":"SIMILARITY"}],"features":[{"type":"Chain","location":{"start":{"value":1,"modifier":"EXACT"},"end":{"value":484,"modifier":"EXACT"}},"description":"Nuclear rim protein 1","featureId":"PRO_0000202586"},{"type":"Transmembrane","location":{"start":{"value":145,"modifier":"EXACT"},"end":{"value":165,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Transmembrane","location":{"start":{"value":252,"modifier":"EXACT"},"end":{"value":272,"modifier":"EXACT"}},"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]},{"type":"Region","location":{"start":{"value":416,"modifier":"EXACT"},"end":{"value":457,"modifier":"EXACT"}},"description":"Disordered","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Compositional bias","location":{"start":{"value":430,"modifier":"EXACT"},"end":{"value":440,"modifier":"EXACT"}},"description":"Polar residues","evidences":[{"evidenceCode":"ECO:0000256","source":"SAM","id":"MobiDB-lite"}]},{"type":"Modified residue","location":{"start":{"value":3,"modifier":"EXACT"},"end":{"value":3,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"18407956"}]},{"type":"Modified residue","location":{"start":{"value":417,"modifier":"EXACT"},"end":{"value":417,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]},{"type":"Modified residue","location":{"start":{"value":474,"modifier":"EXACT"},"end":{"value":474,"modifier":"EXACT"}},"description":"Phosphoserine","evidences":[{"evidenceCode":"ECO:0007744","source":"PubMed","id":"19779198"}]}],"keywords":[{"id":"KW-0472","category":"Cellular component","name":"Membrane"},{"id":"KW-0539","category":"Cellular component","name":"Nucleus"},{"id":"KW-0597","category":"PTM","name":"Phosphoprotein"},{"id":"KW-1185","category":"Technical term","name":"Reference proteome"},{"id":"KW-0812","category":"Domain","name":"Transmembrane"},{"id":"KW-1133","category":"Domain","name":"Transmembrane helix"}],"references":[{"referenceNumber":1,"citation":{"id":"8923743","citationType":"journal article","authors":["Boskovic J.","Soler-Mira A.","Garcia-Cantalejo J.M.","Ballesta J.P.G.","Jimenez A.","Remacha M.A."],"citationCrossReferences":[{"database":"PubMed","id":"8923743"},{"database":"DOI","id":"10.1002/(sici)1097-0061(199610)12:13%3c1377::aid-yea35%3e3.0.co;2-r"}],"title":"The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames.","publicationDate":"1996","journal":"Yeast","firstPage":"1377","lastPage":"1384","volume":"12"},"referencePositions":["NUCLEOTIDE SEQUENCE [GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 96604 / S288c / FY1679","type":"STRAIN"}]},{"referenceNumber":2,"citation":{"id":"9169867","citationType":"journal article","authors":["Jacq C.","Alt-Moerbe J.","Andre B.","Arnold W.","Bahr A.","Ballesta J.P.G.","Bargues M.","Baron L.","Becker A.","Biteau N.","Bloecker H.","Blugeon C.","Boskovic J.","Brandt P.","Brueckner M.","Buitrago M.J.","Coster F.","Delaveau T.","del Rey F.","Dujon B.","Eide L.G.","Garcia-Cantalejo J.M.","Goffeau A.","Gomez-Peris A.","Granotier C.","Hanemann V.","Hankeln T.","Hoheisel J.D.","Jaeger W.","Jimenez A.","Jonniaux J.-L.","Kraemer C.","Kuester H.","Laamanen P.","Legros Y.","Louis E.J.","Moeller-Rieker S.","Monnet A.","Moro M.","Mueller-Auer S.","Nussbaumer B.","Paricio N.","Paulin L.","Perea J.","Perez-Alonso M.","Perez-Ortin J.E.","Pohl T.M.","Prydz H.","Purnelle B.","Rasmussen S.W.","Remacha M.A.","Revuelta J.L.","Rieger M.","Salom D.","Saluz H.P.","Saiz J.E.","Saren A.-M.","Schaefer M.","Scharfe M.","Schmidt E.R.","Schneider C.","Scholler P.","Schwarz S.","Soler-Mira A.","Urrestarazu L.A.","Verhasselt P.","Vissers S.","Voet M.","Volckaert G.","Wagner G.","Wambutt R.","Wedler E.","Wedler H.","Woelfl S.","Harris D.E.","Bowman S.","Brown D.","Churcher C.M.","Connor R.","Dedman K.","Gentles S.","Hamlin N.","Hunt S.","Jones L.","McDonald S.","Murphy L.D.","Niblett D.","Odell C.","Oliver K.","Rajandream M.A.","Richards C.","Shore L.","Walsh S.V.","Barrell B.G.","Dietrich F.S.","Mulligan J.T.","Allen E.","Araujo R.","Aviles E.","Berno A.","Carpenter J.","Chen E.","Cherry J.M.","Chung E.","Duncan M.","Hunicke-Smith S.","Hyman R.W.","Komp C.","Lashkari D.","Lew H.","Lin D.","Mosedale D.","Nakahara K.","Namath A.","Oefner P.","Oh C.","Petel F.X.","Roberts D.","Schramm S.","Schroeder M.","Shogren T.","Shroff N.","Winant A.","Yelton M.A.","Botstein D.","Davis R.W.","Johnston M.","Andrews S.","Brinkman R.","Cooper J.","Ding H.","Du Z.","Favello A.","Fulton L.","Gattung S.","Greco T.","Hallsworth K.","Hawkins J.","Hillier L.W.","Jier M.","Johnson D.","Johnston L.","Kirsten J.","Kucaba T.","Langston Y.","Latreille P.","Le T.","Mardis E.","Menezes S.","Miller N.","Nhan M.","Pauley A.","Peluso D.","Rifkin L.","Riles L.","Taich A.","Trevaskis E.","Vignati D.","Wilcox L.","Wohldman P.","Vaudin M.","Wilson R.","Waterston R.","Albermann K.","Hani J.","Heumann K.","Kleine K.","Mewes H.-W.","Zollner A.","Zaccaria P."],"citationCrossReferences":[{"database":"PubMed","id":"9169867"},{"database":"DOI","id":"10.1038/387s075"}],"title":"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.","publicationDate":"1997","journal":"Nature","firstPage":"75","lastPage":"78","volume":"387"},"referencePositions":["NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":3,"citation":{"id":"24374639","citationType":"journal article","authors":["Engel S.R.","Dietrich F.S.","Fisk D.G.","Binkley G.","Balakrishnan R.","Costanzo M.C.","Dwight S.S.","Hitz B.C.","Karra K.","Nash R.S.","Weng S.","Wong E.D.","Lloyd P.","Skrzypek M.S.","Miyasato S.R.","Simison M.","Cherry J.M."],"citationCrossReferences":[{"database":"PubMed","id":"24374639"},{"database":"DOI","id":"10.1534/g3.113.008995"}],"title":"The reference genome sequence of Saccharomyces cerevisiae: Then and now.","publicationDate":"2014","journal":"G3 (Bethesda)","firstPage":"389","lastPage":"398","volume":"4"},"referencePositions":["GENOME REANNOTATION"],"referenceComments":[{"value":"ATCC 204508 / S288c","type":"STRAIN"}]},{"referenceNumber":4,"citation":{"id":"14562095","citationType":"journal article","authors":["Huh W.-K.","Falvo J.V.","Gerke L.C.","Carroll A.S.","Howson R.W.","Weissman J.S.","O'Shea E.K."],"citationCrossReferences":[{"database":"PubMed","id":"14562095"},{"database":"DOI","id":"10.1038/nature02026"}],"title":"Global analysis of protein localization in budding yeast.","publicationDate":"2003","journal":"Nature","firstPage":"686","lastPage":"691","volume":"425"},"referencePositions":["SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":5,"citation":{"id":"14562106","citationType":"journal article","authors":["Ghaemmaghami S.","Huh W.-K.","Bower K.","Howson R.W.","Belle A.","Dephoure N.","O'Shea E.K.","Weissman J.S."],"citationCrossReferences":[{"database":"PubMed","id":"14562106"},{"database":"DOI","id":"10.1038/nature02046"}],"title":"Global analysis of protein expression in yeast.","publicationDate":"2003","journal":"Nature","firstPage":"737","lastPage":"741","volume":"425"},"referencePositions":["LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]"]},{"referenceNumber":6,"citation":{"id":"15023545","citationType":"journal article","authors":["Wysocka M.","Rytka J.","Kurlandzka A."],"citationCrossReferences":[{"database":"PubMed","id":"15023545"},{"database":"DOI","id":"10.1016/j.yexcr.2003.12.008"}],"title":"Saccharomyces cerevisiae CSM1 gene encoding a protein influencing chromosome segregation in meiosis I interacts with elements of the DNA replication complex.","publicationDate":"2004","journal":"Exp. Cell Res.","firstPage":"592","lastPage":"602","volume":"294"},"referencePositions":["INTERACTION WITH CSM1"]},{"referenceNumber":7,"citation":{"id":"17330950","citationType":"journal article","authors":["Li X.","Gerber S.A.","Rudner A.D.","Beausoleil S.A.","Haas W.","Villen J.","Elias J.E.","Gygi S.P."],"citationCrossReferences":[{"database":"PubMed","id":"17330950"},{"database":"DOI","id":"10.1021/pr060559j"}],"title":"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.","publicationDate":"2007","journal":"J. Proteome Res.","firstPage":"1190","lastPage":"1197","volume":"6"},"referencePositions":["IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"],"referenceComments":[{"value":"ADR376","type":"STRAIN"}]},{"referenceNumber":8,"citation":{"id":"18407956","citationType":"journal article","authors":["Albuquerque C.P.","Smolka M.B.","Payne S.H.","Bafna V.","Eng J.","Zhou H."],"citationCrossReferences":[{"database":"PubMed","id":"18407956"},{"database":"DOI","id":"10.1074/mcp.m700468-mcp200"}],"title":"A multidimensional chromatography technology for in-depth phosphoproteome analysis.","publicationDate":"2008","journal":"Mol. Cell. Proteomics","firstPage":"1389","lastPage":"1396","volume":"7"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]},{"referenceNumber":9,"citation":{"id":"18997772","citationType":"journal article","authors":["Mekhail K.","Seebacher J.","Gygi S.P.","Moazed D."],"citationCrossReferences":[{"database":"PubMed","id":"18997772"},{"database":"DOI","id":"10.1038/nature07460"}],"title":"Role for perinuclear chromosome tethering in maintenance of genome stability.","publicationDate":"2008","journal":"Nature","firstPage":"667","lastPage":"670","volume":"456"},"referencePositions":["FUNCTION","INTERACTION WITH CSM1"]},{"referenceNumber":10,"citation":{"id":"19779198","citationType":"journal article","authors":["Holt L.J.","Tuch B.B.","Villen J.","Johnson A.D.","Gygi S.P.","Morgan D.O."],"citationCrossReferences":[{"database":"PubMed","id":"19779198"},{"database":"DOI","id":"10.1126/science.1172867"}],"title":"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.","publicationDate":"2009","journal":"Science","firstPage":"1682","lastPage":"1686","volume":"325"},"referencePositions":["PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417 AND SER-474","IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]"]}],"uniProtKBCrossReferences":[{"database":"EMBL","id":"X95644","properties":[{"key":"ProteinId","value":"CAA64922.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"Z74137","properties":[{"key":"ProteinId","value":"CAA98655.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"EMBL","id":"BK006938","properties":[{"key":"ProteinId","value":"DAA11769.1"},{"key":"Status","value":"-"},{"key":"MoleculeType","value":"Genomic_DNA"}]},{"database":"PIR","id":"S67625","properties":[{"key":"EntryName","value":"S67625"}]},{"database":"RefSeq","id":"NP_010194.1","properties":[{"key":"NucleotideSequenceId","value":"NM_001180148.1"}]},{"database":"AlphaFoldDB","id":"Q12066","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID","id":"31971","properties":[{"key":"Interactions","value":"69"}]},{"database":"DIP","id":"DIP-1799N","properties":[{"key":"Description","value":"-"}]},{"database":"FunCoup","id":"Q12066","properties":[{"key":"Number of interactors","value":"51"}]},{"database":"IntAct","id":"Q12066","properties":[{"key":"Interactions","value":"10"}]},{"database":"MINT","id":"Q12066","properties":[{"key":"Description","value":"-"}]},{"database":"STRING","id":"4932.YDL089W","properties":[{"key":"Description","value":"-"}]},{"database":"iPTMnet","id":"Q12066","properties":[{"key":"Description","value":"-"}]},{"database":"PaxDb","id":"4932-YDL089W","properties":[{"key":"Description","value":"-"}]},{"database":"PeptideAtlas","id":"Q12066","properties":[{"key":"Description","value":"-"}]},{"database":"EnsemblFungi","id":"YDL089W_mRNA","properties":[{"key":"ProteinId","value":"YDL089W"},{"key":"GeneId","value":"YDL089W"}]},{"database":"GeneID","id":"851469","properties":[{"key":"Description","value":"-"}]},{"database":"KEGG","id":"sce:YDL089W","properties":[{"key":"Description","value":"-"}]},{"database":"AGR","id":"SGD:S000002247","properties":[{"key":"Description","value":"-"}]},{"database":"SGD","id":"S000002247","properties":[{"key":"GeneName","value":"NUR1"}]},{"database":"VEuPathDB","id":"FungiDB:YDL089W","properties":[{"key":"Description","value":"-"}]},{"database":"eggNOG","id":"ENOG502S7S0","properties":[{"key":"ToxonomicScope","value":"Eukaryota"}]},{"database":"HOGENOM","id":"CLU_033252_1_0_1","properties":[{"key":"Description","value":"-"}]},{"database":"InParanoid","id":"Q12066","properties":[{"key":"Description","value":"-"}]},{"database":"OMA","id":"TRSHIFQ","properties":[{"key":"Fingerprint","value":"-"}]},{"database":"OrthoDB","id":"3363151at2759","properties":[{"key":"Description","value":"-"}]},{"database":"BioCyc","id":"YEAST:G3O-29497-MONOMER","properties":[{"key":"Description","value":"-"}]},{"database":"BioGRID-ORCS","id":"851469","properties":[{"key":"hits","value":"0 hits in 10 CRISPR screens"}]},{"database":"PRO","id":"PR:Q12066","properties":[{"key":"Description","value":"-"}]},{"database":"Proteomes","id":"UP000002311","properties":[{"key":"Component","value":"Chromosome IV"}]},{"database":"RNAct","id":"Q12066","properties":[{"key":"moleculeType","value":"protein"}]},{"database":"GO","id":"GO:0031965","properties":[{"key":"GoTerm","value":"C:nuclear membrane"},{"key":"GoEvidenceType","value":"IEA:UniProtKB-SubCell"}]},{"database":"GO","id":"GO:0034399","properties":[{"key":"GoTerm","value":"C:nuclear periphery"},{"key":"GoEvidenceType","value":"HDA:SGD"}],"evidences":[{"evidenceCode":"ECO:0007005","source":"PubMed","id":"14562095"},{"evidenceCode":"ECO:0007005","source":"PubMed","id":"26928762"}]},{"database":"GO","id":"GO:0043007","properties":[{"key":"GoTerm","value":"P:maintenance of rDNA"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"18997772"}]},{"database":"GO","id":"GO:0007096","properties":[{"key":"GoTerm","value":"P:regulation of exit from mitosis"},{"key":"GoEvidenceType","value":"IMP:SGD"}],"evidences":[{"evidenceCode":"ECO:0000315","source":"PubMed","id":"25569132"}]},{"database":"InterPro","id":"IPR018819","properties":[{"key":"EntryName","value":"Nur1/Mug154"}]},{"database":"PANTHER","id":"PTHR28293","properties":[{"key":"EntryName","value":"NUCLEAR RIM PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"PANTHER","id":"PTHR28293:SF1","properties":[{"key":"EntryName","value":"NUCLEAR RIM PROTEIN 1"},{"key":"MatchStatus","value":"1"}]},{"database":"Pfam","id":"PF10332","properties":[{"key":"EntryName","value":"DUF2418"},{"key":"MatchStatus","value":"1"}]}],"sequence":{"value":"MGSNDLINEAYDDSEVVGEERESKSAWMKRWYQLLTSPLDLQLVINEKLEMINWDAYAKSLAKPLGNFLTILFFIIRLLQDNLIKPNYYKLNVKSGAFDLSKSNKLKEFDYLWEISSSFQNNNQFYAFQSWYFVTLRFLNNLFRFTIFILLSLNLYVSCKFMFGYFKTYNLFHLKKEFNSPNLTKHNLKDLSKEYYEDIYKQSLWSMLKHFFRGSRDDGPHVNQNEDEIFFQLRKWIPTNFMINLFVSFSPTAIVFLSFSDVSFTSAIAIVFHQYILDYIITKRFQRSVDDDLILSSAALQEYEDKHIMARINQCSNIDTLSSAMGTRSKTPRIFTTHSLCGEEIREVYNYEKREFEALPKMTESVPGSRETRIKDYGGISQVSDHQSHPIGFHYSPRMSPYYRDKVLDNNLAQSSSNENLEKGGAYLPNQDQNRPSKSLSPLRKTPLSARQKRFEGSEFNVLNKNDINSILRSPKKKKNYHKR","length":484,"molWeight":56887,"crc64":"3785895EA2887E89","md5":"7B6694871B7409B252E631B511152861"},"extraAttributes":{"countByCommentType":{"FUNCTION":1,"SUBUNIT":1,"INTERACTION":1,"SUBCELLULAR LOCATION":1,"MISCELLANEOUS":1,"SIMILARITY":1},"countByFeatureType":{"Chain":1,"Transmembrane":2,"Region":1,"Compositional bias":1,"Modified residue":3},"uniParcId":"UPI000006B713"}}